A method for preparing a shape-customizable acoustic wave-assisted 3D printing high-density cell patch and application

Abstract

This invention relates to the field of biomaterials technology, providing a method and application for the preparation of customizable, acoustically assisted 3D-printed high-density cell patches. The method combines a customized acoustic platform, a signal generator, a power amplifier, and a 3D bioprinter. The sine wave generated by the signal generator is amplified by the power amplifier and converted into an acoustic signal by an interdigital transducer on the acoustic platform. This acoustic signal generates mechanical energy in the bio-ink through a substrate with piezoelectric effects, causing hair follicle-related stem cells to aggregate at the center of the printing platform, thereby bioprinting a high-density cell patch. By changing the bio-ink containing different cell types and altering the printing parameters and shape design, high-density cell patches in the form of discs, grids, or vascular structures can be prepared. The high-density cell patch prepared by this invention mimics the high density of cells in the human body and has a customizable shape, effectively promoting hair follicle regeneration and hair growth.

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