Sphingobium yanoikuyae cbs w-4 and use thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- BIOLOGY INST OF HEBEI ACAD OF SCI
- Filing Date
- 2026-03-26
- Publication Date
- 2026-06-19
Smart Images
Figure CN122235003A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of environmental microbiology technology, specifically relating to a sphingomyelin bacterium CBSW-4 and its applications. Background Technology
[0002] With the rapid development of industrialization and intensive agriculture, soil environmental problems are becoming increasingly severe, especially heavy metal pollution, soil salinization, soil-borne diseases, and soil fertility decline due to excessive use of chemical fertilizers, which seriously restrict the green and sustainable development of agriculture. Traditional soil remediation and improvement methods, such as physicochemical remediation, while effective, are often costly, prone to damaging soil structure, and may even introduce secondary pollution. Therefore, seeking an environmentally friendly, cost-effective, and efficient green remediation technology has become a current research hotspot.
[0003] Microbial remediation, a technology utilizing microorganisms with specific functions for soil improvement and ecological restoration, has shown great application potential due to its advantages such as low cost, environmental friendliness, and ability to restore soil ecological functions. Ideal soil functional microorganisms need to possess multiple excellent characteristics, such as: the ability to survive and colonize in heavy metal-stressed environments; the ability to improve soil nutrients by secreting organic acids and enzymes to convert insoluble silicon, phosphorus, and potassium in the soil into bioavailable forms that can be absorbed by plants, reducing fertilizer use and improving soil fertility. Furthermore, their tolerance to acids, alkalis, and salts determines their adaptability to complex soil environments; and if they can antagonize soil-borne pathogens, they can synergistically promote plant health.
[0004] Currently, although numerous single-function heavy metal-tolerant bacteria, biocontrol bacteria, or phosphorus and potassium-solubilizing bacteria have been reported, existing strains lack sufficient synergistic multifunctionality, making it difficult to simultaneously meet diverse needs such as heavy metal tolerance, nutrient activation, stress resistance, and disease resistance. Furthermore, their adaptability to complex environments is limited. In particular, there is a lack of efficient microbial germplasm resources for complex degraded soils where heavy metal pollution and soil impoverishment coexist (such as tailings areas and farmland surrounding mining areas). Therefore, the exploration and development of multifunctional microbial strains is of great significance for the development of new biological products and the sustainable improvement and remediation of barrier soils. Summary of the Invention
[0005] The purpose of this invention is to provide a *Sphingomonas* CBSW-4 strain that is both tolerant to heavy metals and highly efficient at releasing silicon, solubilizing phosphorus, and dissolving potassium. When applied to heavy metal-contaminated soil, it can effectively alleviate heavy metal stress on plants, promote plant growth, and improve soil quality.
[0006] The present invention adopts the following technical solution: A type of sphingomyelin ( Sphingobacteriumsp.) CBSW-4 was deposited at the China General Microbiological Culture Collection Center on May 27, 2025, with accession number CGMCC No. 34683, located in Beijing, China.
[0007] Furthermore, the *Sphingosporobacter CBSW-4* not only has the ability to tolerate the heavy metal cadmium, but also the ability to tolerate the heavy metals lead, copper, chromium, cobalt, nickel, zinc and manganese.
[0008] Furthermore, the *Sphingosporobacter CBSW-4* possesses the ability to release silicon, dissolve phosphorus, and solubilize potassium.
[0009] Furthermore, the *Sphingomonas CBSW-4* can grow well at pH 5-9, NaCl concentration 0-4%, and temperature 15-40℃.
[0010] Furthermore, the *Sphingosporobacter CBSW-4* can antagonize *Verticillium wilt* of cotton and *Sclerotium solani* of corn.
[0011] Furthermore, the *Sphingomyelinus* CBSW-4 can promote the growth of wheat under cadmium stress and improve soil fertility.
[0012] Application of the above-mentioned Sphingomyelin Bacillus CBSW-4 in promoting plant growth and improving soil quality.
[0013] Application of the above-mentioned Sphingomyelin Bacillus CBSW-4 in the ecological restoration of heavy metal contaminated soil, tailings soil, acidic soil or alkaline soil.
[0014] Application of the above-mentioned Sphingomyces CBSW-4 in the preparation of bio-organic fertilizer, soil conditioner and artificial substrate.
[0015] The beneficial effects of this invention are as follows: (1) The Sphingomonas CBSW-4 provided by the present invention has the characteristics of being resistant to heavy metals cadmium, lead, copper, chromium, cobalt, nickel, zinc and manganese. It can be efficiently applied to the ecological restoration of soils contaminated by heavy metals and soils in tailings areas, and significantly reduce the environmental risks of heavy metals.
[0016] (2) The sphingomyelin Bacillus CBSW-4 of the present invention can release silicon, solubilize phosphorus and potassium in soil, improve soil nutrient supply, and tolerate pH 5~9, NaCl 0~4% and 15~40℃ environment. It has good repair and improvement effects on acidic, alkaline and different salinity soils.
[0017] (3) The Sphingomonas CBSW-4 of the present invention can effectively promote plant growth and improve soil quality. It can be used to prepare bio-organic fertilizer, soil conditioner and artificial substrate, and has the dual value of ecological restoration and agricultural production increase.
[0018] (4) This invention can provide excellent strain resources for the development of functional microorganisms that release silicon, solubilize phosphorus and potassium, as well as for the research and development of new bio-organic fertilizers, soil conditioners, artificial substrates, etc., and has practical economic and social benefits and broad application prospects. Attached Figure Description
[0019] Figure 1 Phylogenetic tree of Sphingosine monoclonal antibody CBSW-4.
[0020] Figure 2 For different Cd 2+ Growth curves of strain CBSW-4 at different concentrations.
[0021] Figure 3 This is a diagram showing the dynamic changes in silicon release during the culture of Sphingosine monophosphate Bacillus CBSW-4.
[0022] Figure 4 The effect of pH on the growth of strain CBSW-4.
[0023] Figure 5 The effect of NaCl on the growth of strain CBSW-4.
[0024] Figure 6 The effect of temperature on the growth of strain CBSW-4. Detailed Implementation
[0025] The present invention will be further described below with reference to the embodiments and accompanying drawings. The scope of protection of the present invention is not limited to the embodiments, and any modifications made by those skilled in the art within the scope defined by the claims also fall within the scope of protection of the present invention.
[0026] Example 1: Isolation, identification and preservation of Sphingosporobacter CBSW-4 The isolated samples were obtained from the rhizosphere soil of plants in an iron tailings mining area in Tangshan. Sampling followed a random and uniform sampling principle, and fresh samples were collected using sterile bags. After mixing the collected soil samples, 10 g was weighed and added to an Erlenmeyer flask containing 90 mL of sterile water. The flask was then placed in a shaker at 180 r / min for 15 min to prepare 10 g of the rhizosphere soil. -1 A soil solution of a certain concentration was then diluted sequentially to a concentration gradient of 10. -3 10 -4 10 -5Soil solutions of varying concentrations were prepared. 100 μL of each solution was evenly spread onto LB agar containing 10 mg / L cadmium ions and incubated at 30°C for 48 h. Well-grown colonies were selected, and purified strains were obtained through repeated streaking. These were then progressively inoculated onto LB agar containing 50, 100, 150, and 200 mg / L cadmium ions. Strains with good cadmium tolerance were selected, and streaking was repeated 2-3 times until pure cultures were obtained. These pure cultures were then transferred to LB slant agar and stored at 4°C for later use.
[0027] Culture medium formulation: (1) LB medium: 10 g tryptone, 5 g yeast extract, 10 g NaCl, 1000 mL water, pH 7.2~7.5 (15~20 g agar needs to be added to solid medium).
[0028] (2) Cadmium-resistant LB liquid medium: Based on the above LB medium, add cadmium chloride (analytical grade) according to the cadmium ion content.
[0029] The isolated cadmium-resistant strain was streaked onto LB solid medium and incubated at 30°C for 48 h. Morphological characteristics such as colony shape and color were observed. The colonies were light yellow, with a smooth surface, irregular edges, opaque, a raised center, and flattened edges, and were easily picked up. Observation under optical and scanning electron microscopes revealed that the bacteria were short rods with clear outlines, existing independently, uniformly plump, with a smooth surface, no surface granular polymers or flagella, and no obvious unevenness at the edges.
[0030] The strain CBSW-4 is Gram-negative and does not produce spores; it is oxidase-positive, does not produce indole, does not hydrolyze proteins or gelatin, is negative for VP test, and positive for nitrate reduction; it can oxidize various sugars to produce acid under aerobic conditions, such as d-arabinose, cellobiose, fructose, galactose, glucose, lactose, maltose, sucrose, and xylose; it cannot ferment to produce acid under anaerobic or facultative anaerobic conditions.
[0031] Total DNA was extracted from CBSW-4 strain using a bacterial genomic DNA extraction kit. Using this DNA as a template, universal 16S rDNA primers were used for amplification: 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'). Agarose gel electrophoresis revealed a band of approximately 1500 bp. The PCR amplification products were sent to Shanghai Sangon Biotech Co., Ltd. for sequencing.
[0032] The sequencing results were submitted to NCBI for homology analysis with existing 16S rDNA sequences in the database. 16S rDNA gene sequences of closely related strains were selected from Genebank, and a phylogenetic tree was constructed using MEGA 7.0. The results showed a close association with *Sphingosine monocytogenes* (…). Sphingobacterium The sequence identity percentage of sp.) IAE112 reached 99.80%. The comparison sequences used for phylogenetic tree construction were selected from the comparison results, and a phylogenetic tree was constructed using MEGA 7.0 software (see...). Figure 1 Based on the morphological and physiological-biochemical characteristics of the strain, strain CBSW-4 was identified as *Sphingomonas*. Sphingobacterium sp.).
[0033] Strain CBSW-4 was deposited on May 27, 2025, at the China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, with accession number CGMCCNo. 34683.
[0034] Example 2: Effects of different cadmium concentrations on the growth of strain CBSW-4
[0035] like Figure 2 As shown, with Cd 2+ With increasing cadmium concentration, the growth of strain CBSW-4 was significantly inhibited, and the growth period was gradually prolonged. The rapid growth phase (T2) of the 50 mg / L cadmium ion treatment group was delayed by 48 h compared to the T1 group without cadmium ion treatment, and the OD (October Resultant) was observed after 60 h of growth. 600 The values were similar and showed the same trend, all initially increasing, reaching a peak at 60 h, and then slowly decreasing. The 100 mg / L cadmium ion treatment group T3, however, only began entering the logarithmic growth phase after 60 h, and the OD value after growth stabilized... 600 The values were also lower than those of the T1 and T2 treatment groups. The T4 treatment group, with a cadmium concentration of 150 mg / L, only began to grow slowly after 156 h, and the OD value was lower. 600 The value gradually increased. However, in treatments with high concentrations of cadmium ions (200 mg / L and 250 mg / L), the growth of the strain was significantly inhibited, and the growth curves for these two treatments were flat, with OD values gradually increasing. 600 The values were all below 0.5, indicating no complete logarithmic growth or a steady slowdown in growth. Bacterial growth curves can reflect the growth response of bacteria under specific environments, providing information on bacterial growth, metabolism, and environmental adaptability. Furthermore, by analyzing the growth curves of strains under different cadmium concentrations in culture media, we can also explore the growth response of the strains under cadmium stress.
[0036] Example 3: Tolerance of strain CBSW-4 to copper, lead, and chromium
[0037] Lead acetate (Pb) was separated using ultrapure water.2+ Copper sulfate (Cu) 2+ ), potassium dichromate (Cr 6+ ), cobalt chloride (Co) 2+ ), zinc sulfate (Zn 2+ Nickel sulfate (Ni) 2+ ) and manganese chloride (Mn 2+ The metal ion solutions were prepared at a concentration of approximately 100 mM and used as stock solutions. In subsequent experiments, specific amounts of each metal ion stock solution were added to LB medium to produce Pb concentrations of 5 mg / L, 15 mg / L, 30 mg / L, 50 mg / L, 100 mg / L, 200 mg / L, 300 mg / L, 400 mg / L, 600 mg / L, 800 mg / L, and 1000 mg / L, respectively. 2+ Cu 2+ Cr 6+ Co 2+ Zn 2+ Ni 2+ and Mn 2+ The strain CBSW-4 was sterilized at 121℃, plated, and inoculated onto different culture media. Its tolerance to heavy metal ions was determined based on the growth of the strain.
[0038] The results are shown in Table 1. Strains CBSW-4 were able to thrive in a solution containing 300 mg / L copper ions (Cu). 2+ 400 mg / L lead ions (Pb) 2+ 15 mg / L chromium ions (Cr) 6+ 50 mg / L Cobalt 2+ 800 mg / L zinc ions (Zn) 2+ 100 mg / L nickel ions Ni 2+ and 600 mg / L manganese ions Mn 2+ Normal growth in the culture medium indicates that strain CBSW-4 is resistant to Pb. 2+ Cu 2+ Cr 6+ Co 2+ Zn 2+ Ni 2+ and Mn 2+ It also exhibits a certain degree of tolerance, indirectly indicating that strain CBSW-4 can be used for the remediation of soils contaminated with these metal ions.
[0039] Table 1. Tolerance of strain CBSW-4 to heavy metal ions .
[0040] Example 4: Dynamic changes in silica release during the culture of Sphingomonas CBSW-4 Leaching and desilication medium: glucose 10 g, KH2PO4 0.2 g, MgSO4·7H2O 0.2 g, NaCl 0.2 g, CaCl2·2H2O 0.2 g, potassium feldspar 1.0 g, CaCO3 5 g, water 1000 mL, pH 7.0~7.2.
[0041] Seed culture of strain CBSW-4 was prepared using LB liquid medium, and the bacterial concentration was adjusted to 1×10⁻⁶. 9 The seed culture was inoculated into the leaching and desilication medium at a concentration of CFU / mL (2% v / v), with a volume of 50 mL (100 mL in a shake flask). The medium was incubated at 30℃ and 120 r / min on a shaker for 9 days. Uninoculated leaching and desilication medium served as a blank control. Each treatment was repeated three times. Samples were taken every 24 h, centrifuged at 8000 r / min for 5 min, and the supernatant was filtered through a 0.22 μm filter. The available silicon content in the fermentation broth was determined using the NYT1121.15-2006 silicomolybdenum blue colorimetric method to determine the dynamic silicon release capacity of the silicon-releasing strain.
[0042] The results show (see) Figure 3 As the culture time increased, the content of available silicon in the supernatant gradually increased, then leveled off and stopped increasing. It reached its maximum value of 35.38 mg / L after 7 days of culture, which was 2.69 times that of the uninoculated control. This indicates that strain CBSW-4 can significantly improve the conversion rate of silicon-containing minerals and promote the release of available silicon.
[0043] Example 5: Determination of Phosphate-Solubilizing and Potassium-Solubilizing Abilities of Sphingosine Bacillus CBSW-4
[0044] NBRIP phosphate-solubilizing medium: glucose 10 g, Ca3(PO4)2 5 g, (NH4)2SO4 0.5 g, MgSO4·7H2O 0.25 g, KCl 0.2 g, MgCl2·6H2O 5 g, agar 15 g, 0.4% bromophenol blue 6 mL, water 1000 mL, pH 7.0~7.2.
[0045] Potassium-solubilizing medium: 10 g sucrose, 1 g Na2HPO4, 0.5 g (NH4)2SO4, 1 g MgSO4·7H2O, 0.2 g yeast extract, 0.1 g NaCl, 0.1 g CaCO3, 0.005 g FeCl3, 5 g potassium feldspar, 1000 mL water, pH 7.0~7.2.
[0046] Seed culture of strain CBSW-4 was prepared and inoculated onto NBRIP phosphate-solubilizing medium and potassium-solubilizing medium, respectively. After culturing in a constant temperature incubator at 30℃ for 2 days, its growth and the presence of a lysis zone were observed.
[0047] The calculation method is as follows: Solubility = Diameter of the solubility zone D / Diameter of the colony d.
[0048] The results showed that CBSW-4 colonies exhibited distinct transparent zones around their periphery in both NBRIP phosphate-solubilizing and potassium-solubilizing media, with D / d values of 1.35 and 1.56, respectively, indicating that they possess certain phosphate-solubilizing and potassium-solubilizing abilities.
[0049] Example 6: Stress resistance of Sphingomyelin Bacillus CBSW-4
[0050] The stress resistance of strain CBSW-4 was investigated based on its growth under different pH, NaCl concentrations, and temperatures. Specifically, the pH range was set to 3–10, NaCl concentration to 0–8%, and temperature to 15–40℃. Seed culture of strain CBSW-4 was inoculated into 30 mL LB liquid shake flasks at a 2% (v / v) inoculation rate. The flasks were then cultured on a shaker at 30℃ and 180 r / min for 2 days. The OD of the fermentation broth was then measured. 600 As an indicator for detecting bacterial growth.
[0051] The results showed that the growth of strain CBSW-4 was less affected by pH, and it could grow well in the pH range of 5–9 (see [link to results]). Figure 4 When pH < 5 or pH > 9, the growth of the strain is significantly inhibited. Strain CBSW-4 grows well at pH 5–9, indicating strong acid and alkali tolerance and environmental adaptability, making it suitable for acidic or alkaline soils and offering significant advantages in the development of soil conditioners. Furthermore, since most soils in iron ore tailings areas are alkaline, this strain has excellent application advantages in the ecological reclamation of tailings area soils.
[0052] Strain CBSW-4 grows well in the range of 0–4% NaCl (see [reference needed]). Figure 5 When the NaCl concentration was 6%, the growth of the strain was strongly inhibited. Bacteria can only grow within a certain salt concentration range. When the concentration is high, the osmotic pressure in the fermentation broth exceeds the osmotic pressure inside the bacteria, causing cell shrinkage due to water loss, thus inhibiting the physiological and biochemical reactions within the microorganisms and suppressing their growth and reproduction. This strain, CBSW-4, can grow well in the range of 0-4% NaCl, indicating that it has good salt tolerance and can be maintained unaffected during the remediation of saline-alkali soils.
[0053] The results showed that strain CBSW-4 grew well in the temperature range of 15℃ to 37℃, exhibiting good wide temperature adaptability (see...). Figure 6When the temperature is below 15℃ or above 40℃, the number of strains decreases sharply, indicating that the growth of the strain is strongly inhibited. A suitable temperature is essential for bacterial fermentation; the optimal culture temperature for strain CBSW-4 is 30℃.
[0054] In summary, this indicates that strain CBSW-4 possesses strong acid and alkali resistance, salt tolerance, and a wide temperature range, demonstrating its strong tolerance to adverse conditions.
[0055] Example 7 Antagonism of strain CBSW-4 against plant pathogens The antagonistic effect of strain CBSW-4 against plant pathogens was determined using the plate confrontation method. Pathogen fragments were inoculated at the center of PDA plates, and strain CBSW-4 was inoculated at equal intervals on both sides of the fragments. The plates were incubated at 25°C for 48 h, with three replicates. The absence of normal pathogen growth near strain CBSW-4 indicated an antagonistic effect. The results (see Table 2) showed that strain CBSW-4 had a significant antagonistic effect against Verticillium wilt of cotton and small leaf spot of corn.
[0056] Table 2. Antagonistic activity of strain CBSW-4 against some pathogenic fungi. .
[0057] The antifungal activity of CBSW-4 against *Verticillium wilt* and *Sclerotinia serratifolia* was determined using the growth rate method. CBSW-4 was cultured in LB liquid medium for 2 days, centrifuged, and the supernatant was filtered through a 0.22 μm sterile filter. A certain amount of the sterile filtrate was added to PDA medium at 40–45℃, mixed well, and poured into 9 cm petri dishes. A blank control was used without the addition of sterile filtrate. After the medium solidified, a bacterial block of the plant pathogen was inoculated in the center of each plate, with three replicates, and incubated at 28℃. The colony diameter was measured using the cross-crossing method, and the inhibition rate was calculated. The results showed that the inhibition rate of CBSW-4 against both *Verticillium wilt* and *Sclerotinia serratifolia* was not less than 52%.
[0058] Inhibition rate (%) = (Coronavirus colony diameter of control bacteria - Coronavirus colony diameter of treated bacteria) / (Coronavirus colony diameter of control bacteria - Coronavirus block diameter) × 100.
[0059] Example 8: Effects of Sphingomyelin-Bacillus CBSW-4 on wheat growth and soil fertility under cadmium stress
[0060] Seeds used in the test: Jimai 958, purchased from a seed company in Hebei Province. Mature, plump, and uniformly sized healthy seeds were selected, and the seed surface was disinfected with 0.1% potassium permanganate solution for 30 minutes. The seeds were then rinsed three times with distilled water and air-dried for later use.
[0061] Table 3. Main chemical components of iron tailings sand samples .
[0062] Strain strain CBSW-4 was inoculated into LB liquid medium and cultured at 30℃ and 180 r / min in a shaker until the logarithmic growth phase. The number of bacterial cells per milliliter of liquid medium was determined by plate count. Sterilized wheat seeds were soaked in warm water for 12 h and then sown in soil containing 10 mg / kg cadmium ions (a mixture of 30% garden soil, 40% iron tailings sand, and 30% vermiculite; the iron tailings sand was sourced from an iron tailings dam in Chengde, and its main components are shown in Table 3). Three treatment groups were set up: a control group (CK) with added water, a control group (CK) with 1×10⁻⁶ bacterial cells, and a control group (CK) with added water. 7 T1 group with CFU / mL bacterial culture and 1×10 8 The T2 group (CFU / mL) was used, with 20 replicates for each treatment group. The soil was kept moist daily. After the wheat seedlings had grown for 40 days, the biomass of root length and seedling length, as well as the contents of available nitrogen, available silicon, available phosphorus, and available potassium in the soil were measured.
[0063] Table 4. Effects of strain CBSW-4 on wheat growth under cadmium stress .
[0064] The results showed (see Table 4) that treatment with CBSW-4 bacterial solution had a good effect on alleviating wheat stress under 10 mg / kg cadmium ion stress. Adding 1×10⁻⁶ CBSW-4 bacterial solution to the soil... 7 In group T1, with CFU / mL bacterial suspension, seedling length increased by 7.84% and root length increased by 4.03% compared to the control (CK), but the differences were not statistically significant. However, the soil supplemented with 1×10⁻⁶ CFU / mL bacterial suspension showed no significant difference. 8 In the T2 group with CFU / mL bacterial solution, the seedling length and root length were significantly increased compared with the pure water CK group, increasing by 20.25% and 18.60% respectively, indicating that the addition of CBSW-4 bacterial solution can alleviate the stress of heavy metal cadmium on wheat to a certain extent and promote wheat growth.
[0065] Table 5. Effects of Sphingomyelin-Bacillus CBSW-4 on soil fertility (unit: %) .
[0066] Soil nutrient content is crucial for plant growth and development. Table 5 shows that application of CBSW-4 bacterial solution significantly increased the contents of available nitrogen, available silicon, available phosphorus, and available potassium in the soil. Compared with the control, treatment T2 was superior to treatment T1, increasing these contents by 17.55%, 23.72%, 31.70%, and 20.83%, respectively. This further demonstrates that application of CBSW-4 bacterial solution can effectively improve soil fertility.
[0067] The present invention has been described in detail with reference to the above embodiments. It should be noted that the above embodiments are merely illustrative examples. Without departing from the spirit and essence of the present invention, those skilled in the art can devise various alternatives and improvements to the present invention, all of which should be understood to be within the scope of protection of the present invention.
Claims
1. A type of Sphingosine monocytogenes ( Sphingobacterium sp.) CBSW-4, characterized in that, Its accession number is CGMCCNo.34683.
2. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, This strain is tolerant to the heavy metal cadmium.
3. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, This strain is resistant to heavy metals such as lead, copper, chromium, cobalt, nickel, zinc, and manganese.
4. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, This strain has the ability to release silicon, dissolve phosphorus, and solubilize potassium.
5. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, It can grow well at pH 5-9, NaCl concentration 0-4%, and temperature 15-40℃.
6. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, It can antagonize cotton wilt fungus and corn leaf blight fungus.
7. The *Sphingomonas CBSW-4* according to claim 1, characterized in that, It can promote the growth of wheat under cadmium stress and improve soil fertility.
8. The application of the *Sphingomyelin-Bacillus CBSW-4* as described in claim 1 in promoting plant growth and improving soil quality.
9. The application of Sphingomyelin Bacillus CBSW-4 as described in claim 1 in the ecological restoration of heavy metal contaminated soil, tailings soil, acidic soil or alkaline soil.
10. The application of Sphingomyelin Bacillus CBSW-4 as described in claim 1 in the preparation of bio-organic fertilizers, soil conditioners, and artificial substrates.