A method and system for hierarchical stable preservation of quinoa germplasm resources and a special packaging unit thereof

Abstract

This invention belongs to the field of germplasm resource preservation technology and discloses a method, supporting system, and dedicated packaging unit for the graded and stable preservation of quinoa germplasm resources. Addressing industry pain points such as large genotypic differences in quinoa germplasm, susceptibility to moisture absorption and mold growth, rapid viability decay, and incomplete traceability chains, this invention constructs a three-in-one technical system of "method-system-packaging," with core components including full-indicator grading upon entry into the storage facility, three-dimensional personalized preservation path decision-making, low-damage segmented drying and homogenization, high-barrier precise packaging, closed-loop management and stratified sampling within the storage facility, and tamper-proof traceability through an event ledger. This invention achieves precise, standardized, and traceable preservation of quinoa germplasm, significantly reducing the risks of viability decay, mold growth, and pests during long-term seed storage. It is suitable for long-term preservation, off-site backup, and shared utilization of quinoa germplasm resources.

Description

Technical Field

[0001] This invention belongs to the field of agricultural biotechnology and germplasm resource preservation, specifically involving a graded and stable preservation method for seeds of quinoa (Chenopodium quinoa) and its closely related materials, a supporting modular preservation system and a special packaging unit. It is particularly suitable for the standardized, large-scale, and traceable preservation of quinoa germplasm resource banks, and belongs to the core technical field of agricultural germplasm resource protection and utilization. Background Technology

[0002] Quinoa, as a distinctive coarse grain crop characterized by high protein, complete nutrition, and strong stress resistance, is of great significance for variety improvement, industrial upgrading, and food security through the protection and preservation of its germplasm resources. Current methods for preserving crop germplasm resources generally follow the "Technical Specifications for the Management of Crop Germplasm Resource Banks" (NY / T 1604-2008) and the "Procedures for Seed Testing of Crops" (GB / T 3543.1~3543.7-1995), employing common low-temperature and low-humidity preservation methods. However, due to its unique biological characteristics, quinoa germplasm exhibits significant adaptability deficiencies under existing technological systems. The core challenges are as follows:

[0003] 1. Genotypic differences lead to processing difficulties: The grain size, thousand-grain weight and exocarp structure of seeds of different quinoa genotypes vary significantly. Common drying processes are prone to uneven drying speed, poor consistency of moisture content within batches, high embryo damage rate, and accelerated viability decline during long-term storage.

[0004] 2. High risk of mold and pests: Quinoa seeds have loose outer pericarps and their surface easily absorbs moisture. When general packaging is not sufficiently barrier or the seal is not tight, they are very easy to become damp. In addition, some insect eggs can still survive in normal low-temperature environments, which can lead to pests and cause the seed quality to be ruined.

[0005] 3. Limited and untargeted preservation approaches: The lack of personalized preservation approaches based on germplasm quality, scarcity, and preservation duration, and the "one-size-fits-all" approach, can easily lead to insufficient preservation of core and scarce germplasm and waste of conventional germplasm preservation resources.

[0006] 4. Incomplete traceability system: Information such as test data, process parameters, and abnormal handling is recorded in a scattered manner and is not integrated and linked. The traceability chain is incomplete and it is difficult to meet the requirements of reliable auditing and sharing of germplasm resources.

[0007] 5. Low level of standardization in operation: There is a lack of quinoa-specific technical specifications in the pretreatment, drying, and packaging processes, resulting in large human error and poor batch-to-batch consistency.

[0008] Currently, there is no comprehensive three-in-one graded and stable preservation technology system for quinoa germplasm biological characteristics that integrates "method-system-packaging". There is an urgent need to develop a dedicated preservation technology that is adapted to the specific characteristics of quinoa, can improve preservation stability, reduce risks, and achieve full-process traceability, so as to fill the technological gap in the standardized preservation of quinoa germplasm resources. Summary of the Invention

[0009] 1. Technical problems to be solved

[0010] To address the core problems in existing quinoa germplasm resource preservation technologies, such as poor drying uniformity, high risk of mold and pests, single preservation path, incomplete traceability chain, and low standardization, this invention provides a graded and stable preservation method for quinoa germplasm resources. It also includes a modular preservation system and dedicated packaging units, forming a closed-loop technology to achieve precise, standardized, and traceable preservation of quinoa germplasm resources. This improves long-term preservation stability, reduces the risk of viability decay, mold, and pests, ensures reliable auditing and full-process traceability of the preservation process, and provides technical support for the large-scale management of quinoa germplasm resource banks.

[0011] 2 Technical Solution

[0012] The core innovation of this invention lies in "tiered decision-making, precise processing, closed-loop control, and ledger traceability," coupled with a customized system and packaging, forming a three-in-one quinoa germplasm resource tiered preservation technology system encompassing "method-system-packaging." The core technical route is a six-step progressive closed-loop process, as detailed below:

[0013] Step S1: Full-Indicator Testing and Grading for Storage Quinoa seeds to be stored first undergo manual and mechanical cleaning to remove large impurities and shriveled seeds. Then, a full-indicator test is performed on six core indicators: moisture content (MC), germination rate (G), germination rate after accelerated aging (AA-G), impurity rate, moldy seed rate, insect egg / live insect detection, and contamination rate / abnormal seed rate. Based on the storage quality grading thresholds in Table 1, the seeds are graded into three levels: A, B, and C. Level A represents high-quality, rare core germplasm; Level B represents conventionally used germplasm; and Level C represents germplasm awaiting treatment. A MaterialID (unique germplasm material identifier) ​​and BatchID (storage batch number) are generated to achieve unique identification of the germplasm.

[0014] Table 1. Quality grading thresholds for quinoa germplasm resources entering the database (A / B / C)

[0015]

[0016] Note: The values ​​in parentheses are the basic thresholds, which can be used when there are no special requirements; for scarce / ultra-small samples, non-destructive testing methods can be used, and the alternative methods and reasons must be recorded; Grade C seeds are not necessarily eliminated, and can be re-graded after being cleaned, dried, purified and other treatments to meet the standards.

[0017] Step S2: Personalized Preservation Path Decision. A three-dimensional path decision model based on "grade + duration + scarcity" is established. According to seed grade results, target preservation duration, germplasm scarcity, and regeneration availability, at least one preservation path from P1 (medium-term), P2 (long-term), and P3 (backup / ultra-long-term) is determined from the preservation path parameter table in Table 2. Core path decision rules: For Grade A core scarce germplasm, the P2 (long-term) + P3 (backup) dual path is preferred; for Grade B conventional germplasm, P1 (medium-term) is preferred, and it can be upgraded to P2 as needed; for Grade C germplasm, after re-cleaning, re-drying, and other treatments to meet standards, the corresponding path is selected based on actual quality. Path identifiers are generated to provide a basis for subsequent temperature zone bank selection and sampling frequency setting.

[0018] Table 2. Parameters for the preservation pathway of quinoa germplasm resources (P1 / P2 / P3)

[0019]

[0020] Note: Humidity control prioritizes dew point index to avoid relative humidity errors caused by temperature changes; the sampling cycle for core rare / abnormal germplasm is shortened by 50%, while the cycle for routine germplasm without abnormalities can be extended by 50%; cross-repository migration requires consistent LocationID hierarchical coding for easy traceability; LN2 gas phase is stored in liquid nitrogen gas phase.

[0021] Step S3: Pretreatment and Low-Damage Drying Homogenization. This step is the core pretreatment process for quinoa germplasm preservation, consisting of two parts: pretreatment and segmented drying. It addresses the issues of poor drying uniformity and embryo damage in quinoa.

[0022] 1. Physical pretreatment: Insect / mildew control treatment is carried out by freezing at -20℃ for 4 hours or low-power microwave irradiation. The treatment method is non-destructive and ensures that the seed germination rate decreases by ≤3% after treatment, thereby reducing the risk of mold and pests from the source.

[0023] 2. Segmented Drying for Homogenization: A three-stage drying process is adopted, consisting of "pre-drying - sealed buffering and settling - final drying". Pre-drying (30℃, 2h) removes free moisture from the seed surface, sealed buffering and settling (25℃, RH40%, 4h) eliminates the internal moisture content gradient of the seed, and final drying (35℃, 3h) precisely controls the moisture content to the target range corresponding to the storage path. This gradient heating process is suitable for the characteristics of quinoa seeds with thin seed coats and loose pericarps, avoiding seed coat cracking and embryo damage.

[0024] 3. Baseline determination: After drying, the moisture content and germination rate of the seeds are retested to form the storage baseline MC0 (moisture content) / G0 (germination rate). The moisture content deviation of seeds within the batch is required to be ≤0.5%.

[0025] Step S4: High-Barrier Packaging and Sealing Inspection. This step is the core process for preventing moisture regain in quinoa germplasm preservation, achieving triple protection through "dedicated packaging + precise sealing + dual quality inspection":

[0026] 1. Packaging Selection: Based on the storage path and seed quality, select the corresponding standardized special low moisture permeability multi-layer barrier packaging unit (small sample / medium sample / large sample), with core indicators conforming to the specifications in Table 3;

[0027] 2. Precise packaging: The seeds are packed into the packaging unit and vacuum-sealed (vacuum degree ≥ -0.09MPa) or sealed with nitrogen / argon inert gas (residual oxygen ≤ 2%). The inert gas environment can further inhibit the survival of insect eggs and the reproduction of microorganisms.

[0028] 3. Dual quality inspection: The sealed packages are subjected to heat seal strength test and leakage test in sequence. The test standards are implemented according to Table 3. The unqualified products are immediately reworked and resealed. The qualified products generate PackageID (unique number of each package) and are bound to the unique identification carrier of the packaging unit.

[0029] Table 3. Quality Inspection Technical Specifications for Quinoa Germplasm Packaging Materials and Sealing

[0030]

[0031] Note: The packaging material is an agricultural-grade environmentally friendly barrier material, odorless and does not react chemically with quinoa seeds; sealing strength and leakage testing are critical control points (KCPs) and require 100% testing; vacuum sealing has no residual oxygen requirements.

[0032] Step S5: In-Storage Closed-Loop Control and Risk-Stratified Sampling Inspection. This step is the core link in the in-store risk control of quinoa germplasm preservation, realizing full-process risk management through "closed-loop environmental control + stratified sampling inspection + CAPA closed-loop disposal".

[0033] 1. Storage: Store the qualified seeds in the corresponding temperature zone warehouse and record the LocationID (hospital address hierarchical code). The temperature zone warehouse performs closed-loop precise control of temperature, humidity / dew point. The fluctuation range is implemented according to Table 2. Dew point is used as the humidity control indicator first.

[0034] 2. Risk-stratified sampling: Based on the basic cycle in Table 2, a risk-level-based stratified sampling strategy is adopted (risk level is determined by storage years, grading results, and historical anomaly records). Sampling is conducted on packaging sealing and seed moisture content (MC). t ), germination rate (G) t ), calculate the change in moisture content ΔMC=MC t -MC0, the decrease in germination rate ΔG=G0-G t Increase the sampling rate for Grade A / P3 pathway germplasm, and appropriately reduce the sampling rate for Grade B / P1 pathway germplasm with no abnormal records.

[0035] 3. Abnormal CAPA Closed-Loop Handling: If the sampling results trigger the risk threshold in Table 4, immediately implement the CAPA (Corrective and Preventive Action) closed-loop process: When the warning threshold is triggered, implement isolation and retesting + shorten the sampling cycle by 50% + increase the monitoring frequency; when the serious threshold is triggered, implement full batch isolation + cause analysis + re-drying / repackaging / redirection, etc. + full batch re-inspection + release isolation for qualified products / scrap for unqualified products.

[0036] Table 4. Risk Thresholds for Quinoa Germplasm Preservation and CAPA Closed-Loop Disposal Table

[0037]

[0038] Note: Early warning indicates "corrective and preventative measures," requiring only enhanced monitoring; severe cases indicate "mandatory measures," necessitating the activation of the entire CAPA closed-loop process; the proportion of batch non-compliance can be appropriately adjusted based on the scarcity of germplasm.

[0039] Step S6: Event Ledger-Based Trusted Traceability. This step is the core data link in quinoa germplasm preservation. It constructs an append-only, tamper-proof event ledger record structure to achieve full-process auditability and traceability.

[0040] 1. Ledger Structure: It includes nine core information categories: identity identifier, classification decision, warehousing baseline, process parameters, quality inspection results, sampling inspection records, abnormal closure, audit fields, and anti-tampering fields. The list of core fields is shown in Table 5.

[0041] 2. Immutability Mechanism: Key information in all operational steps is appended and written in chronological order, and cannot be deleted or modified; adjacent event records are linked through chained hash verification, and key events (hierarchical, encapsulated, CAPA handling) are subject to digital signature verification (bound to the operator's account).

[0042] 3. Multi-terminal sharing: The ledger data supports querying on multiple terminals such as computers, tablets, and mobile phones, and realizes cross-database data synchronization, which facilitates the sharing and management of quinoa germplasm resources and meets the requirements of credible auditing.

[0043] Table 5. List of Event Ledger Record Fields (Traceability and Tamper-Proof Audit)

[0044]

[0045] Note: All event records are appended in chronological order and cannot be deleted or modified; PrevHash is the CurrHash of the previous event, realizing chain association; core events must be accompanied by a Signature (operator's digital signature).

[0046] 3.3.3 Beneficial Effects

[0047] This invention, targeting the biological characteristics of quinoa germplasm resources, designs a three-in-one graded and stable preservation technology system integrating "method-system-packaging," which has the following six significant advantages compared to existing general preservation technologies:

[0048] 1. Three-dimensional path decision-making with strong adaptability: By designing personalized preservation paths through a three-dimensional model of "grading + age + scarcity", it solves the pain points of resource waste or insufficient preservation of core germplasm caused by the "one-size-fits-all" preservation of existing technologies. It enables quinoa germplasm of different qualities and scarcity to be matched with the optimal preservation strategy, improving the long-term preservation success rate by ≥30%.

[0049] 2. Low-damage treatment with good viability retention: The process of gradient heating and segmented drying + closed buffering and static setting effectively eliminates moisture content gradients, with an intra-batch moisture content deviation of ≤0.5%, which is suitable for the thin and easily damaged characteristics of quinoa seeds and significantly reduces seed embryo damage; the non-destructive physical pretreatment ensures that the germination rate decreases by ≤3% while killing insects and preventing mold, thus slowing down the rate of viability decay during long-term storage.

[0050] 3. Triple protective packaging to prevent moisture and pests: Special low moisture permeability multi-layer barrier packaging + vacuum / inert gas sealing + dual quality inspection of heat seal strength / leakage test, to achieve precise control of packaging moisture and oxygen permeability, significantly reducing the risk of seed moisture and mold, and the inert gas environment further inhibits the survival of insect eggs, solving the problem of pests in quinoa storage from the source;

[0051] 4. Closed-loop risk management and high preservation stability: Closed-loop precise control of temperature, humidity and dew point in the storage room + stratified sampling based on risk level + CAPA closed-loop treatment realizes full-process risk monitoring of the preservation process. Abnormal situations can be quickly identified and dealt with in a timely manner, reducing the abnormal scrap rate of germplasm preservation to below 5%.

[0052] 5. Ledger-based traceability, trustworthy and auditable: The event ledger enables the appending and writing of data throughout the entire process and ensures its immutability. The chain hash + digital signature verification method guarantees the integrity of the traceability chain, which solves the problems of scattered and easily tampered records in existing technologies, reduces the management risks of mislabeling and mixing, and meets the requirements of trustworthy auditing and shared utilization of germplasm resources.

[0053] 6. A three-in-one system with a high degree of standardization: The supporting modular preservation system enables data interconnection and automated operation at each stage. The dedicated packaging unit is of standardized specifications, compatible with automated packaging equipment, reducing human error and facilitating the large-scale and standardized management of the quinoa germplasm resource bank. It has good industrialization and promotion value.

[0054] 3.4 Terminology and Judgment Standards

[0055] 1. Baseline for entry into storage: The moisture content (MC0), germination rate / vigor (G0) and other index values ​​obtained from the first verification after the sample has completed pretreatment and packaging are used as the comparison benchmark for subsequent monitoring in storage. The deviation of seed moisture content within a batch is required to be ≤0.5%.

[0056] 2. ΔG (germination rate decrease): defined as ΔG = G0 - G t Where G0 is the baseline germination rate upon entry into the warehouse, G t The germination rate for a specific sampling inspection is expressed as a percentage decrease, used to evaluate the degree of seed vigor decline.

[0057] 3. ΔMC (Moisture Content Change): Defined as ΔMC = MC t -MC0, where MC0 is the baseline moisture content at the time of entry into the reservoir, and MC t The moisture content is from a random sampling inspection, used to assess the risk of seed re-moistening.

[0058] 4. Warning Threshold / Severity Threshold: The warning threshold indicates a slight abnormality in the seed storage status, requiring immediate isolation and retesting, as well as increased monitoring frequency; the severity threshold indicates a serious abnormality in the seed storage status, requiring immediate entry into the CAPA closed-loop process for mandatory handling.

[0059] 5. Risk stratified sampling: The sampling ratio and frequency are dynamically adjusted according to seed grade (A / B / C), storage path (P1 / P2 / P3), historical abnormal records and germplasm scarcity. The sampling ratio of core scarce germplasm is increased, while the sampling ratio of conventional germplasm without abnormal records is appropriately reduced.

[0060] 6. Packaging Batch: A packaged collection formed from the same quinoa germplasm material, the same process parameters, the same packaging material batch number, and the same operating shift, serving as the basic unit for sampling inspection and traceability.

[0061] 7. CAPA Process: A closed-loop process for corrective and preventive actions, including five core steps: isolation, cause analysis, implementation of action, re-verification, and release from isolation / disposal. All operations must be recorded in the event ledger to achieve closed-loop management.

[0062] 8. Chained Hash Verification: The algorithm uses the hash value (CurrHash) of the previous event as the pre-hash value (PrevHash) of the next event, so that all events are linked in a chain. If the data of any event is tampered with, all subsequent hash values ​​will become invalid, thus achieving data immutability. 3.5 Description of the attached figures

[0064] Figure 1: System Modular Technical Architecture Diagram. The system adopts a four-layer modular interconnected architecture, divided into a perception layer, processing layer, control layer, and traceability layer from top to bottom. The core functional modules include an inbound inspection module, a pre-processing module, a drying homogenization module, a packaging quality inspection module, a temperature zone warehouse control module, a risk monitoring module, and a reliable traceability module. Each module achieves automatic data uploading and sharing through a data interaction platform, eliminating information silos.

[0065] Figure 2: Structure diagram of the dedicated packaging unit. The packaging unit is an integrated structure with low moisture permeability and multi-layer barrier, without splicing gaps, and is suitable for vacuum / inert gas encapsulation; the environmental status indicator component is a visual design, which can quickly determine whether the internal environment of the packaging is abnormal; three standardized specifications are suitable for quinoa germplasm with different scarcity and storage quantities, meeting the diverse preservation needs of the resource bank.

[0066] Figure 3: Core Technology Roadmap. The technology roadmap is a six-step progressive closed-loop process. Key data from each step is uploaded to the event ledger in real time. If an anomaly is found during sampling, the CAPA closed-loop handling is immediately triggered, and the handling result is fed back to the event ledger, forming a complete technical closed loop of "operation-recording-monitoring-handling-re-recording". This ensures that the storage process is risk-free and fully traceable.

[0067] Figure 4: Three-dimensional path decision model diagram of "Grading + Duration + Scarcity". Taking germplasm grading, target preservation duration, and germplasm scarcity / reproducibility as three-dimensional inputs, the decision model outputs three preservation paths, P1 / P2 / P3, to achieve personalized preservation strategy matching for different germplasms and avoid resource waste or insufficient preservation.

[0068] Figure 5: CAPA Closed-Loop Handling Flowchart. The entire anomaly handling process is detailed, from threshold triggering, batch isolation, cause analysis, handling implementation to verification, forming a complete closed loop. All operations are recorded in the event ledger and digitally signed to ensure the handling process is traceable and auditable.

[0069] Figure 6: Chain Hash Verification Logic Diagram of the Event Ledger. This diagram illustrates the chain hash structure of the event ledger, where each event block contains the hash value of the previous block, forming an immutable chain connection. Digital signatures are added to critical events to further ensure data authenticity and operational traceability. 3.6 Detailed Implementation

[0071] The technical solution of the present invention will be described in detail below with reference to specific embodiments. The embodiments are only used to explain the present invention and are not intended to limit the scope of protection of the present invention.

[0072] Example 1: Long-term + backup dual-path preservation of Grade A quinoa core germplasm (P2+P3)

[0073] 1. Entry Inspection and Grading: Take the core rare quinoa germplasm QX-2026-015 (non-renewable), clean and test: moisture content 5.8%, germination rate 96%, germination rate after accelerated aging 88%, impurity rate 0.1%, moldy grain rate 0%, no insect eggs / live insects, and mixed rate 0.05%. According to Table 1, it is judged as Grade A, and MaterialID=QX-2026-015 and BatchID=QX-2026-015-B01 are generated.

[0074] 2. Path decision: The target preservation period is ≥30 years. As core rare and non-renewable germplasm, according to the three-dimensional decision model, a dual preservation path is determined: P2 (long-term, -20±3℃) + P3 (backup, -80℃), with each path divided into 50 medium samples.

[0075] 3. Pretreatment and Drying Homogenization: Physical insect control was performed by freezing at -20℃ for 4 hours, which reduced the germination rate by 1%. Subsequently, segmented drying was carried out: pre-drying (30℃, 2 hours) → sealed buffering (25℃, RH 40%, 4 hours) → final drying (35℃, 3 hours). After drying, the moisture content was retested at 5.6%, the germination rate at 96%, and the batch moisture content deviation was 0.3%, forming the baseline for storage: MC0=5.6%, G0=96%.

[0076] 4. High-barrier packaging and quality inspection: Select a sample-grade low-moisture-permeable multi-layer barrier packaging unit, fill it with seeds, and then perform nitrogen-purified packaging (residual oxygen 0.8%). The heat seal strength test is 22N / 15mm, the leakage test is qualified, and PackageID=PK-015-0001~0100 is generated and bound to the RFID tag.

[0077] 5. Storage and Control: Store 50 samples in a -20℃ temperature zone (LocationID=PY-01-F037-L03-P12~61) and 50 samples in a -80℃ backup zone (LocationID=BF-02-F010-L05-P01~50). The dew points in the storage zones should be controlled at -22℃ and -32℃ respectively, with a temperature fluctuation of ±2℃.

[0078] 6. Sampling Inspection and Traceability: Following the P2 / P3 path requirements, P2 involves sampling inspections of sealing performance every 12 months, moisture content every 24 months, and vitality every 36 months; P3 involves sampling inspections of sealing performance every 12 months, moisture content every 24 months, and vitality every 60 months. All sampling inspection data is appended to the event ledger in chronological order, adjacent records are linked through chained hash verification, and key operations are digitally signed to achieve full-process traceability.

[0079] Example 2: Mid-term preservation of Grade B quinoa germplasm (P1)

[0080] 1. Inspection and grading upon entry into the warehouse: Take the conventional quinoa germplasm LM-2026-028 (easy to regenerate and frequently used), and after cleaning, test the following: moisture content 7.5%, germination rate 85%, germination rate after accelerated aging 68%, impurity rate 0.8%, moldy grain rate 0.2%, no insect eggs / live insects, and mixed rate 0.3%. According to Table 1, it is judged as Grade B, and a corresponding identification label is generated.

[0081] 2. Path decision: With a target preservation period of 5 years, easy regeneration, and frequent use, the P1 medium-term preservation path (5±2℃) is determined.

[0082] 3. Pretreatment and drying homogenization: Low-power microwave irradiation is used for mildew prevention treatment, and the moisture content is controlled at 7.0% after segmented drying to form the baseline for warehousing;

[0083] 4. High-barrier packaging and quality inspection: Select a large sample single-layer high-barrier packaging unit, vacuum seal it, test the sealing strength to be 18N / 15mm, pass the leakage test, generate a PackageID and bind a QR code;

[0084] 5. In-store control and sampling inspection: After entering the storage area, the RH inside the storage area is controlled at 28% at 5℃. According to the P1 path requirements, the sealing and moisture content are sampled every 12 months, and the viability is sampled every 24 months. If there are no abnormalities, the monitoring is carried out at the regular frequency, and the data is recorded in the event ledger in real time to facilitate the rapid retrieval and utilization of germplasm resources.

[0085] Example 3: CAPA Closed-Loop Processing for P2 Storage Path Moisture Reversion Early Warning

[0086] Taking the P2 pathway quinoa germplasm QX-2026-015 from Example 1 as an example, routine sampling was conducted in the 12th month after its storage. The specific CAPA closed-loop processing procedure is as follows:

[0087] 1. Warning Triggered: Moisture content MC of PackageID=PK-015-0001 is sampled. t =6.3%, ΔMC=+0.7%, triggering the reabsorption warning threshold in Table 4 (ΔMC≥0.5%), the system immediately issues a warning, and staff isolate the batch of 50 samples to the isolation warehouse, and the warning information is recorded in the event ledger in real time;

[0088] 2. Expanded Inspection and Investigation: A full expanded inspection was conducted on 50 packaging units from the same packaging batch. Three units were found to have failed the leakage test, triggering the critical threshold (batch non-compliance ≥ 5%). The system automatically generated CAPA_ID=CAPA-2027-003 and started the CAPA closed-loop process.

[0089] 3. Treatment Implementation: The 50 isolated germplasm samples were re-dried (30℃, 1h) to reduce the moisture content to 5.7%; new batch packaging materials were used, and nitrogen purging was performed again for resealing. All 50 packaging units underwent heat seal strength and leakage tests, and all passed (heat seal strength 21N / 15mm, residual oxygen 0.9%).

[0090] 4. Re-verification: The moisture content of 50 germplasm samples was found to be 5.7%, and the leakage test was passed. The CAPA treatment was deemed effective. The re-verification results were recorded in the event log and digitally signed.

[0091] 5. Release from quarantine and subsequent monitoring: The batch is returned to the original -20℃ temperature zone warehouse to release from quarantine. The system automatically shortens the sampling cycle of the batch by 50% (moisture content is sampled every 6 months). All handling procedures are added to the event ledger in chronological order to form a complete and auditable chain of evidence.

[0092] Example 4: Application of dedicated packaging units in rare small-sample germplasm

[0093] For ultra-scarce quinoa germplasm samples (less than 20g, non-renewable), a special low-moisture-permeable, multi-layer barrier packaging unit for small samples was selected, with the following specific applications:

[0094] 1. Packaging Selection: Sample-sized packaging units with a double-barrier structure, moisture permeability (WVTR) = 0.2 g / m³. 2 • 24h, Oxygen permeability OTR = 0.3cc / m 2 • 24h·atm, the bag opening has an automated heat-sealing structure with a heat-sealing strength ≥20N / 15mm;

[0095] 2. Identification and Indication: Equipped with a unique RFID identification carrier (uniquely bound to PackageID) to support long-distance data reading; built-in irreversible humidity indicator (changes from blue to pink when humidity > 6%) and traceable temperature tag (records duration and number of overheating incidents) for rapid visual detection of the environment inside the packaging;

[0096] 3. Packaging and storage: Nitrogen-purified packaging (0.7% residual oxygen) was used. After packaging, the seeds were stored in the P3 backup storage path (-80℃). After 12 months of storage, random sampling was conducted. There was no leakage in the packaging, no discoloration of the humidity indicator, no over-temperature record on the temperature label, the seed moisture content was 5.5% (ΔMC=-0.1%), the viability was maintained at 95%, and the storage condition was good.

Claims

1. A method for graded and stable preservation of quinoa germplasm resources, characterized in that, The steps are as follows: (1) Full index testing and grading upon entry into storage: Full index testing of quinoa seeds to be stored was carried out, including moisture content, germination rate, germination rate after accelerated aging, impurity rate, moldy grain rate, detection of insect eggs / live insects, and contamination rate. Based on the test results, the seeds were graded into three levels: A, B, and C. Among them, level A is high-quality core rare germplasm, level B is conventionally used germplasm, and level C is germplasm to be treated. (2) Personalized preservation path decision: Based on the seed grading results, target preservation period, germplasm scarcity, and regeneration availability, a three-dimensional path decision model of "grading + period + scarcity" was constructed to determine at least one of the medium-term P1, long-term P2, and backup / ultra-long-term P3 preservation paths. Among them, P1 is preservation in the 4-10℃ temperature range, P2 is preservation in the -18℃ and below temperature range, and P3 is preservation in the -80℃ and below temperature range. The decision rule is: level A core rare germplasm preferentially selects the P2+P3 dual path, level B Grade 1 conventional germplasm selection P1 path, Grade C seeds are matched with the corresponding path after treatment to meet the standard; (3) Pretreatment and low-damage drying homogenization: First, the seeds are subjected to non-destructive physical pest control / mildew prevention pretreatment to ensure that the germination rate of the seeds decreases by ≤3% after treatment; then, the segmented drying process of "pre-drying-sealed buffering and static-final drying" is implemented to eliminate the internal moisture content gradient of the seeds, so that the moisture content of the seeds reaches the target range of the corresponding storage path, forming the storage baseline MC0 / G0, and the moisture content deviation within the batch is ≤0.5%; (4) High barrier packaging and full inspection of sealing: The seeds are put into a special low moisture permeability multi-layer barrier packaging unit, vacuum sealed or inert gas replacement packaging, and after completion, the heat seal strength test is carried out on the packaging + Leakage test double quality inspection, and can only enter the next stage after passing the test; (5) Closed-loop control and risk-stratified sampling inspection in the warehouse: Packed qualified seeds are put into the corresponding temperature zone warehouse, and the temperature, humidity and dew point in the warehouse are controlled in a closed loop; a stratified sampling strategy based on storage years, grade results and historical abnormal records is adopted, and the packaging sealing, seed moisture content and seed vigor are sampled according to the preset cycle, and the moisture content change ΔMC and the germination rate decrease ΔG are calculated; if the sampling results trigger the warning / serious threshold, the CAPA corrective and preventive measures closed-loop process is immediately executed. The process includes at least isolation, re-drying, repackaging, path transfer or scrapping; (6) Event ledger-style trusted traceability: each packaging unit is configured with a unique identifier, and the key parameters, operation events, test results and abnormal handling records of steps (1)-(5) are added to the immutable event ledger in chronological order. Adjacent records are linked by chain hash verification, and key events are verified by digital signature, so as to achieve full auditability and traceability.

2. The method according to claim 1, characterized in that, In step (1), grade C seeds can be re-tested and graded after re-cleaning, re-drying, and purification; rare / ultra-small germplasm samples should be tested using non-destructive methods, and alternative methods and reasons should be recorded in the event ledger.

3. The method according to claim 1, characterized in that, In step (3), the pretreatment is either freezing at -20℃ for 4 hours or low-power microwave irradiation; the closed buffering is carried out in a constant temperature and humidity environment of 25℃ and RH40%, and the settling time is dynamically adjusted according to the quinoa seed particle size and thousand-grain weight, with a basic time of 2-6 hours.

4. The method according to claim 1, characterized in that, The segmented drying process in step (3) is as follows: pre-drying at 30℃ for 2 hours to remove free moisture from the seed surface; sealing and buffering for 4 hours to eliminate the moisture content gradient inside the seed; and finally drying at 35℃ for 3 hours to precisely control the moisture content to the target range.

5. The method according to claim 1, characterized in that, In step (4), the moisture permeability (WVTR) of the dedicated packaging unit is ≤1.0 g / m³. 2 • 24h, Oxygen permeability (OTR) ≤ 1.0cc / m 2 • 24h·atm; Residual oxygen in the packaging after inert gas replacement ≤2%, heat seal strength ≥15N / 15mm; Vacuum sealing does not have residual oxygen requirements.

6. The method according to claim 5, characterized in that, In step (4), the moisture permeability (WVTR) of the dedicated packaging unit is preferably ≤0.2 g / m³. 2 • 24h, Oxygen permeability OTR preferably ≤0.5cc / m 2 • 24h atm; after inert gas replacement, the residual oxygen in the packaging is preferably ≤1%, and the heat seal strength is preferably ≥20N / 15mm.

7. The method according to claim 1, characterized in that, In step (5), the humidity control of the temperature zone warehouse prioritizes the dew point index, and the dew point fluctuation of different storage paths is ≤±2℃; when the warning threshold is triggered, the warehouse is isolated for retesting and the sampling cycle is shortened by 50%; when the severe threshold is triggered, the CAPA full-process disposal is initiated immediately; the CAPA disposal results need to be verified by re-inspection, and the isolation can be lifted and the warehouse can be returned after the re-inspection is qualified. Non-conforming products are scrapped or transferred to a storage path in accordance with regulations, and the full process record is digitally signed and included in the event ledger.

8. The method according to claim 1, characterized in that, In step (6), the event ledger should include at least germplasm identification, grading path information, entry baseline indicators, process / packaging parameters, quality inspection / sampling results, abnormal handling records, and operator / time stamp. The ledger data supports multi-terminal query and cross-warehouse synchronization.

9. A quinoa germplasm resource conservation system for implementing the method of any one of claims 1-8, characterized in that, It adopts a modular and interconnected architecture, including an inbound inspection module, a pre-processing module, a drying and homogenization module, a packaging quality inspection module, a temperature zone warehouse control module, a risk monitoring module, and a reliable traceability module; Each module enables automatic data interaction and uploading. The traceability module generates an event ledger bound to the unique identifier of the packaging unit, and performs tamper-proof control through append writing, chain hash verification, and / or digital signature verification. The system uses the three-level grading results of quinoa germplasm (A / B / C) as the core basis, and matches the corresponding storage path, drying process, packaging standard, and sampling strategy, forming a unified inventive concept with the method described in claim 1.

10. The system according to claim 9, characterized in that, The inbound inspection module is equipped with multi-indicator integrated testing equipment, supporting simultaneous testing of all indicators and automatic data uploading; the temperature zone control module is equipped with a temperature, humidity / dew point closed-loop control system, supporting real-time early warning of anomalies and visualization of historical data; the risk monitoring module has a built-in stratified sampling strategy and CAPA process triggering mechanism to realize automated risk identification, handling reminders, and closed-loop recording.

11. A dedicated packaging unit for quinoa germplasm resources for implementing the method of any one of claims 1-8, characterized in that, To adapt to the low moisture permeability multilayer barrier integrated structure of vacuum / inert gas encapsulation, it includes a low moisture permeability multilayer barrier bag, a unique identification carrier, and an environmental status indicator component; the bag meets the barrier performance threshold of claim 5 and is compatible with automated heat sealing equipment; the unique identification carrier is uniquely bound to the event ledger PackageID; The status indicator component can provide real-time feedback on the temperature and humidity status inside the packaging, facilitating rapid visual inspection; the packaging unit matches the hierarchical storage path described in claim 1, forming a unified inventive concept.

12. The packaging unit according to claim 11, characterized in that, The low-permeability, multi-layer barrier bag is made of agricultural-grade environmentally friendly material and comes in three standardized sizes: small sample (≤50g), medium sample (50-500g), and large sample (>500g), to suit quinoa germplasm of different scarcity and storage requirements. The bag opening has a heat-sealing structure with a heat-sealing strength of ≥20N / 15mm.

13. The packaging unit according to claim 11, characterized in that, The unique identification carrier is a QR code, RFID, or NFC tag; the environmental status indicator component includes an irreversible humidity indicator that changes color when humidity > 6%, and / or a traceable temperature tag that records the duration and number of times the temperature exceeds the limit.

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