Polyaniline and polyacrylic acid modified silk screen carbon electrode adiponectin detection method
By constructing a polyaniline-polyacrylic acid composite modification layer and an antibody immobilization mechanism on a carbon mesh electrode, the contradiction between conductivity and antibody immobilization ability was resolved, enabling high-sensitivity and high-reproducibility adiponectin detection in complex serum environments.
Patent Information
- Application Number
- CN202610423183.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-01
- Publication Date
- 2026-06-26
AI Technical Summary
In existing technologies, the modified interface of the carbon mesh electrode is difficult to simultaneously achieve both conductivity and antibody stability in the electrochemical detection of adiponectin in human serum, resulting in insufficient detection sensitivity and repeatability, especially in complex serum environments.
A polyaniline and polyacrylic acid-modified carbon mesh electrode was used to form a polyaniline-polyacrylic acid composite modification layer through electrochemical activation. This layer constructed a conductive transport network and provided antibody immobilization sites. The antibody immobilization was activated by N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The non-specific sites were blocked by bovine serum albumin, thereby reducing non-specific adsorption.
This improved the signal response, sensitivity, and repeatability of adiponectin detection, reduced background noise and serum interference, and achieved highly sensitive and repeatable electrochemical detection.