A method for determining the rate of penetration of exogenous additives in tobacco leaves

Abstract

This invention relates to the field of tobacco processing technology, specifically a method for determining the penetration rate of exogenous additives in tobacco leaves, comprising the following steps: 1) tobacco leaf pretreatment; 2) sample loading of tobacco leaves and exogenous additives: tobacco leaves and exogenous additives are placed in two thin-film mixing tanks, one for experimental groups and one for control groups; 3) application of exogenous additives; 4) heat detection: a calorimeter is used to detect and record the heat changes throughout the entire process, including the application of the exogenous additives to the tobacco leaves, their penetration into the tobacco leaves, and their reaction with reactants in the lower tank after passing through the tobacco leaves; 5) calculation of the penetration rate. The advantages of this invention are: by accurately detecting the heat changes of exogenous additives during the penetration process in tobacco leaves, the penetration rate of tobacco leaves can be characterized in real time and dynamically. It is applicable to the detection of the penetration rate of different types of exogenous additives in tobacco leaves, has a wide range of applications, and is simple and efficient.

Description

Technical Field

[0001] This invention relates to the field of tobacco processing technology, and more specifically to a method for determining the penetration rate of exogenous additives in tobacco leaves. Background Technology

[0002] In tobacco processing, exogenous additives, such as enzymes and flavorings, are often added to improve the quality of tobacco leaves. Commonly used enzymes include cellulase, pectinase, protease, and amylase. These enzymes penetrate the tobacco leaves and react. Cellulase and pectinase are mainly used to break down cellulose and pectin in tobacco leaves, improving their physical properties, making them softer, and enhancing the processing performance of cigarettes. Protease is mainly used to degrade proteins in tobacco leaves, reducing bitterness and improving the taste. Amylase can break down starch and polysaccharides into simple sugars, enhancing the sweetness of the tobacco leaves. Through enzymatic catalysis, the chemical composition of tobacco leaves can be altered, improving their sensory quality and reducing the formation of certain harmful substances (such as nitrosamines and unburned polycyclic aromatic hydrocarbons) during tobacco processing. Commonly used flavorings include naturally extracted flavorings (such as essential oils and extracts), artificially synthesized flavorings (such as phenylethyl alcohol, ionone, and methyl aminobenzoate), tobacco flavorings, as well as latent flavorings and microcapsule flavorings. These flavorings permeate the tobacco leaves, modifying, supplementing, and highlighting the original tobacco aroma and flavor of high-quality tobacco leaves, and also helping to form and highlight the style characteristics of cigarettes.

[0003] Currently, the application and reaction control of exogenous tobacco additives in the cigarette industry are achieved through feeding machines, flavoring machines, and storage cabinets after flavoring application. Since there is no method to measure the penetration rate of exogenous additives in tobacco leaves, the selection of the reaction time of treated tobacco leaves is mainly based on the sensory quality analysis of tobacco leaves or general technical standards, such as: the storage time of leaves after adding additives should be at least 2 hours (see the "Cigarette Process Specifications" issued by the State Tobacco Monopoly Administration in 2003, pages 18-19). This makes it impossible to accurately control the penetration reaction process of exogenous additives in cigarette production. Too long a storage reaction time increases energy consumption, while too short a time will affect the application effect.

[0004] Patent CN111189943A discloses a "method for detecting the glycerol permeation rate in tobacco sheets." The method involves excessively coating the upper surface of the tobacco sheet with glycerol, exposing the lower surface to a closed tank with inlet and outlet ports. The inlet port of the closed tank is connected to a test purge gas, and the outlet port is directly connected to containers containing low-temperature methanol solutions via pipelines. A fixed purge interval is maintained, and gas chromatography is used to detect the glycerol content in each methanol solution. Finally, the permeation rate of glycerol in the sample is calculated. However, this method is only suitable for determining the glycerol permeation rate in tobacco sheets. Because there are many types of exogenous additives in tobacco, different additives, such as biological enzyme preparations and tobacco flavoring raw materials, have different boiling points and volatility, making the above method of determining glycerol permeation rate through purge gas and glycerol content unsuitable. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of the existing technology by providing a method for determining the penetration rate of exogenous additives in tobacco leaves. This method can measure the penetration rate of different exogenous additives in tobacco leaves in real time and dynamically. The method has a wide range of applications and is simple and efficient.

[0006] The objective of this invention is achieved through the following technical solution:

[0007] A method for determining the penetration rate of exogenous tobacco additives in tobacco leaves specifically includes the following steps:

[0008] (1) Pretreatment of tobacco leaves: First, the tobacco leaves used to determine the permeation rate are balanced to ensure that the moisture content in the tobacco leaves is uniform; the balanced tobacco leaf samples are made into tobacco sheets with a diameter d of 8.27-11.00 mm, and the thickness h of the samples is measured.

[0009] (2) Sample loading of tobacco leaves and exogenous additives: tobacco leaves and exogenous additives were placed in two film mixing tanks, one for the experimental group and one for the control group. Both film mixing tanks included an upper tank, a middle tank and a lower tank.

[0010] In the experimental group, the upper tank of the thin-film mixing tank contains an exogenous additive solution (concentration C), the lower tank contains a substance that can undergo an exothermic chemical reaction with the exogenous additive, and the middle tank is an empty cavity without any substance. The upper and middle tanks are separated by a diaphragm to prevent the exogenous additive in the upper tank from entering the middle tank. The middle and lower tanks are separated by pretreated tobacco sheets.

[0011] The control group membrane mixing tank was the same as the experimental group except that the solvent added to the upper tank was the same as the solvent used to prepare the exogenous additive solution.

[0012] (3) Application of exogenous additives: The experimental group and the control group film mixing tanks are placed into the inner tank of the calorimeter. After the temperature of the film mixing tank set by the calorimeter reaches the set temperature, the membrane between the upper tank and the middle tank of the experimental group and the control group film mixing tank is punctured at the same time, so that the exogenous additives enter the middle tank and mix with the tobacco.

[0013] (4) Heat detection: A calorimeter is used to detect and record the heat changes throughout the entire process of the external additives being applied to the tobacco sheet, penetrating into the tobacco sheet, and reacting with the reactants in the lower tank after passing through the tobacco sheet;

[0014] (5) Calculation of permeation rate: Based on the time t(t) of the entire process of the exogenous additive permeating the tobacco leaf recorded by the calorimeter, and the diameter d and thickness h of the tobacco leaf, the permeation rate v of the exogenous additive in the tobacco leaf is calculated. The specific calculation formula is as follows:

[0015]

[0016] In this invention, the formula for the penetration rate of exogenous additives in tobacco leaves refers to the calculation method disclosed in invention patent CN111189943A. The difference is that this invention measures the penetration rate using the concentration of exogenous additives.

[0017] In step (1), the tobacco leaves are placed in a constant temperature and humidity environment to equilibrate moisture; the equilibrated tobacco leaf samples are then cut into tobacco sheets with a diameter d of 8.27-11.00 mm using a punch; the thickness h of the samples is measured using a thickness gauge. The constant temperature and humidity environment can be a dynamic moisture adsorption-desorption instrument, a constant temperature and humidity chamber, or a constant temperature and humidity room. The punch can be a simple manual tobacco leaf punch. The thickness gauge can be a portable electronic tobacco leaf thickness measuring instrument.

[0018] The tobacco leaf sample is a sheet-like material, which can be roasted tobacco leaves or tobacco flakes. When it is roasted tobacco leaves, it can be placed on a petri dish and soaked with keratinase to dissolve the cuticle layer of the tobacco leaf epidermis to accelerate penetration.

[0019] The specific structure of the thin-film mixing tank in step (2) is as follows: it includes a base and a sleeve fitted on the base. From bottom to top, the sleeve contains a test smoke sheet holder, a test smoke sheet fixing ring, an intermediate seat, a diaphragm fixing ring, and a diaphragm fixing seat. A test smoke sheet is sandwiched between the test smoke sheet holder and the test smoke sheet fixing ring, and a diaphragm is sandwiched between the diaphragm fixing ring and the diaphragm fixing seat. The test smoke sheet and the diaphragm divide the mixing tank into a lower tank, a middle tank, and an upper tank. The test membrane holder and the test membrane fixing ring are placed on the base, the intermediate seat is directly pressed against the test membrane fixing ring, and the diaphragm fixing ring and the diaphragm fixing seat are supported by the intermediate seat.

[0020] The exogenous additives added to the upper pool in step (2) can be biological enzyme preparations such as pectinase and cellulase, tobacco flavoring liquids such as glycerol and citric acid; the corresponding exothermic reaction substances can be substances rich in pectin such as pectin powder and fruit juice, substances rich in cellulose such as plant cell wall extract and vegetable juice, alkaline substances and other substances that can react with the flavoring liquid to release heat.

[0021] The substance that can undergo an exothermic chemical reaction with the external additives, as mentioned in step (2), can be either solid or liquid.

[0022] The diaphragm is a thin film that carries exogenous additives and does not react with them; the thin film can be a Teflon film.

[0023] The calorimeter mentioned can be a C80 micro calorimeter.

[0024] In this invention, the measurement method and principle of the time t (the time for the exogenous additive to permeate the tobacco leaf during the entire process recorded by the calorimeter) are as follows: The exogenous additive solution will generate heat fluctuations at the moment of contact with the tobacco leaf, and a leading edge will appear on the heat flow diagram. After the exogenous additive solution permeates the tobacco leaf, it will undergo an exothermic reaction with the substances in the lower pool, and a trailing edge will appear on the heat flow diagram. The time between the leading edge and the trailing edge is the time taken for the exogenous additive solution to permeate the tobacco leaf.

[0025] The working principle and process of this invention are as follows: The thin-film mixing cell is enclosed by a calorimeter containing a three-dimensional sensor. This sensor consists of a cylindrical detector array composed of numerous thermocouples, surrounding the thin-film mixing cells of both the experimental and control groups to detect the heat flow of the sample in all directions, exhibiting high sensitivity and accuracy. In the experimental group, the upper chamber of the thin-film mixing cell is filled with an exogenous additive solution, while treated tobacco leaves are placed between the middle and lower chambers. A substance that reacts with the exogenous additive and releases heat is added to the lower chamber. In the control group, the upper chamber of the thin-film mixing cell contains the same amount of exogenous additive solvent as the experimental group, while the other parts of the mixing cell are identical to those in the experimental group. This filling method ensures that the two groups of thin-film mixing cells maintain the same physical quantities, changing only the type of exogenous additive, thereby measuring the change in relative heat during the permeation of the exogenous additive. After the membrane mixing tank is placed in the calorimeter and the temperature stabilizes, the diaphragm is punctured, allowing the exogenous additive solution to enter the middle tank and mix with the tobacco leaves. Heat fluctuations occur during the instant the exogenous additive contacts the tobacco leaves, during its penetration into the layered structure of the tobacco leaves, and during its reaction with the substances in the lower tank. The heat fluctuation at the instant the exogenous additive solution contacts the tobacco leaves will appear as a leading edge on the heat flow graph. After the exogenous additive solution has penetrated the tobacco leaves, it undergoes an exothermic reaction with the substances in the lower tank, resulting in a trailing edge on the heat flow graph. The time between the leading and trailing edges represents the time it takes for the exogenous additive solution to penetrate the tobacco leaves. By recording the reaction between the exogenous additive and the tobacco leaves using the calorimeter, a heat flow curve of the entire process of exogenous additive penetration into the tobacco leaves can be obtained. From the curve, the penetration time of the exogenous additive can be obtained, and thus the penetration rate can be calculated.

[0026] The advantages of this invention are: by accurately detecting the heat change of exogenous additives during the infiltration process of tobacco leaves, the infiltration rate of tobacco leaves can be characterized in real time and dynamically. It is applicable to the detection of the infiltration rate of different types of exogenous additives in tobacco leaves, has a wide range of applications, and is simple and efficient. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of a thin-film mixing tank structure;

[0028] Figure 2 This is a schematic cross-sectional view of the front of the thin-film mixing tank;

[0029] Figure 3 The test results are for Specific Implementation Example 1;

[0030] Figure 4 The test results are for specific embodiment 2;

[0031] Figure 5 The test results are for specific embodiment 3;

[0032] In the diagram: 1. Sleeve; 2. Diaphragm fixing seat; 3. Diaphragm fixing ring; 4. Intermediate seat; 5. Test tobacco sheet fixing ring; 6. Test tobacco sheet fixing seat; 7. Base; 8. Diaphragm; 9. Test tobacco sheet; 10. Lower pool; 11. Middle pool; 12. Upper pool. Detailed Implementation

[0033] To make the above-mentioned objectives, technical solutions, and beneficial effects of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Example 1

[0035] A method for determining the permeation rate of pectinase solution in tobacco leaves in different states is described clearly and completely below with reference to the accompanying drawings:

[0036] (1) Pretreatment of tobacco leaves: Xuchang upper tobacco leaves (B2F) were placed in a dynamic moisture adsorption / desorption apparatus (30℃, 80% RH) for equilibration for 24 hours. After equilibration, the tobacco leaves were removed and cut into round slices slightly larger than the culture dish. To achieve rapid penetration, the upper surface of the tobacco slices was placed on a culture dish filled with a cutinase solution (which can dissolve the cuticle layer of the tobacco leaf epidermis) and placed in a constant temperature and humidity chamber (45℃, 80% RH) for static incubation. The cutinase immersion time was taken as t. x(2) Loading of tobacco leaves and pectinase solution: For the experimental group, the film mixing pool is composed of a lower pool 10, a middle pool 11 and an upper pool 12 arranged from bottom to top. A mixture of 0.1g pectin powder and 0.6ml water is added to the lower pool 10 on the base 7. The treated tobacco leaf 9 (with the lower surface of the tobacco leaf facing up) is placed on the test tobacco leaf fixing seat 6 below the middle seat 4, i.e. on the test tobacco leaf fixing seat 6. Then, a Teflon membrane (i.e., diaphragm 8) fixed by the diaphragm fixing ring 3 is placed on the diaphragm fixing seat 2. 0.7ml pectinase and 0.3ml water (the prepared pectinase concentration is C0) are added to the upper pool 12. For the control group, 1ml water is added to the upper pool 12, and the other parts are the same as the experimental group. (3) Application of pectinase solution and water: First, turn on the power switch of the instrument and set the calorimeter to automatic mode. Start the data recording and processing software, set the ambient temperature required for the reaction of pectinase with tobacco leaves to 45℃, and after the calorimeter stabilizes at the set temperature of 45℃, fill the experimental group and control group of the film mixing tank into the inner tank of the calorimeter respectively. When the substances added to the film mixing tank and the calorimeter reach the same environmental conditions (the heat curve is constant and without fluctuation), press the spiral piercing tool above the film mixing tank with external force to pierce the Teflon membrane of the tank, so that the pectinase solution or water falls into the middle tank 11 and contacts and penetrates the tobacco leaves. (4) Heat detection: Observe the peak value change on the heat flow diagram and measure the time t taken for the first peak - the fine peak - the last peak. y This time is the time it takes for the pectinase solution or water to penetrate from the lower epidermis of the tobacco leaf until it penetrates the upper epidermis and reacts with the pectin powder. (5) Calculation of penetration rate: Based on the thickness h and diameter d of the tobacco leaf, the penetration rate of the pectinase solution with a concentration of C0 in the tobacco leaf can be calculated. Test results are available Figure 3 The calculation results are shown in Table 1.

[0037] Table 1 Calculation results of Example 1

[0038]

[0039] Example 2

[0040] A method for determining the permeation rate of pectinase solutions of different concentrations in tobacco sheets is described clearly and completely below with reference to the accompanying drawings:

[0041] (1) Pretreatment of tobacco sheets: The sheets were placed in a constant temperature and humidity chamber (22℃, 80%RH) to balance the moisture for 24 hours. The sheets were then removed and the thickness h and diameter d of the sheets were measured. (2) Loading of tobacco sheets and pectinase solutions of different concentrations: For the film mixing pool of the experimental group, the mixing pool was composed of a lower pool 10, a middle pool 11 and an upper pool 12 from bottom to top. A mixture of 0.1g pectin powder and 0.6ml water was added to the lower pool 10 on the base 7. The treated sheet (i.e., the test sheet 9) was placed below the middle seat 4, i.e., on the test tobacco sheet fixing seat 6, and then a Teflon membrane (i.e., the diaphragm 8) was placed on the diaphragm fixing seat 2 and fixed with the diaphragm fixing ring 3. 0.7ml pectinase and 0.3ml water or 0.8ml pectinase and 0.2ml water (the prepared pectinase concentration was C) was added to the upper pool 12. x ). For the control group's film mixing pool, add 1 ml of water to the upper pool 12, and the other parts are the same as the experimental group. (3) Application of pectinase solution and water of different concentrations: First turn on the instrument power switch and adjust the calorimeter to the automatic mode. Start the data recording and processing software, set the ambient temperature required for the reaction of pectinase and film to 45℃, and wait for the calorimeter to stabilize to the set temperature of 45℃. Then fill the experimental group and control group of the film mixing pool into the inner tank of the calorimeter respectively. When the substances added to the film mixing pool and the calorimeter reach the same environmental conditions (the heat curve is constant and without fluctuation), press the spiral piercing tool above the film mixing pool with external force to pierce the Teflon membrane of the pool, so that the pectinase solution or water falls into the middle pool 11 and contacts and penetrates the test tobacco film. (4) Heat detection: Observe the peak value change on the heat flow graph and measure the time t of the front peak to the back peak. z This time is the time it takes for the pectinase solution or water to penetrate from one side of the sheet until it reaches the other side and reacts with the pectin powder. (5) Calculation of penetration rate: Based on the thickness h and diameter d of the sheet, the concentration C can be calculated. x (the permeation rate of pectinase solution on the film) Test results are available Figure 4 The calculation results are shown in Table 2.

[0042] Table 2 Calculation results of Example 2

[0043]

[0044] Example 3

[0045] A method for determining the permeation rate of a glycerol-water mixture of a specific concentration in tobacco sheets is described clearly and completely below with reference to the accompanying drawings:

[0046] (1) Pretreatment of tobacco sheets: The sheets were placed in a constant temperature and humidity chamber (22℃, 80%RH) to equilibrate the moisture for 24 hours. The equilibrated sheets were then removed, and the thickness h and diameter d of the sheets were measured. (2) Loading of tobacco sheets and glycerol-water mixture: For the film mixing tank of the experimental group, the mixing tank was composed of a lower tank 10, a middle tank 11 and an upper tank 12 from bottom to top. 0.5 ml of 0.8 mol / L sodium hydroxide solution was added to the lower tank 10 on the base 7. The treated sheet (i.e., test sheet 9) was placed below the middle seat 4, i.e., on the test tobacco sheet fixing seat 6, and then a Teflon membrane (i.e., diaphragm 8) was placed on the diaphragm fixing seat 2, and 0.4 ml of glycerol and 0.8 ml of water (the concentration of the prepared glycerol-water mixture was C) was added to the upper tank 12. y ). For the control group's film mixing pool, 1.2 ml of water was added to the upper pool 12, and the other parts were the same as the experimental group. (3) Application of glycerol-water mixture and water: First, turn on the instrument power switch and adjust the calorimeter to the automatic mode. Start the data recording and processing software, set the ambient temperature required for the reaction of glycerol and the sheet to 30℃, and wait for the calorimeter to stabilize to the set temperature of 30℃. Then, fill the experimental group and the control group of the film mixing pool into the inner tank of the calorimeter respectively. When the substances added to the film mixing pool and the calorimeter reach the same environmental conditions (the heat curve is constant and without fluctuation), press the spiral piercing tool above the film mixing pool with external force to pierce the Teflon membrane of the pool, so that the glycerol-water mixture or water falls into the middle pool 11 and contacts and penetrates the test tobacco sheet. (4) Heat detection: Observe the peak value change on the heat flow graph and measure the time t between the front peak and the back peak. w This time is the time it takes for the glycerol-water mixture or water to begin permeating from one side of the sheet until it penetrates to the other side and reacts with the sodium hydroxide solution. (5) Calculation of permeation rate: Based on the thickness h and diameter d of the sheet, the concentration C can be calculated. y The permeation rate of the glycerol-water mixture in the film Test results are available Figure 5 The calculation results are shown in Table 3.

[0047] Table 3 Calculation results of Example 3

[0048]

[0049] In summary, this invention provides a method for determining the penetration rate of exogenous additives in tobacco leaves. The type and dosage of the exogenous additive, the substances that undergo exothermic chemical reactions with the exogenous additive, and the object being measured can be changed according to process requirements. It can accurately detect the heat changes of the exogenous additive during the penetration process in tobacco leaves, providing real-time and dynamic characterization of the tobacco leaf penetration rate. This method has a wide range of applications and is fast and efficient.

[0050] The above-described embodiments are merely one implementation of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the present invention, and these all fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the appended claims.

Claims

1. A method of determining the rate of penetration of a tobacco exogenous additive into tobacco leaf, characterised in that: Specifically, the following steps are included: (1) Pretreatment of tobacco leaves: First, the tobacco leaves used to determine the permeation rate are balanced to ensure that the moisture content in the tobacco leaves is uniform; the balanced tobacco leaf samples are made into tobacco sheets with a diameter d of 8.27-11.00 mm, and the thickness h of the samples is measured. (2) Sample loading of tobacco leaves and exogenous additives: tobacco leaves and exogenous additives were placed in two film mixing tanks, one for the experimental group and one for the control group. Both film mixing tanks included an upper tank, a middle tank and a lower tank. In the experimental group, an exogenous additive solution with a concentration of C was added to the upper tank of the thin-film mixing tank, a substance that could undergo an exothermic chemical reaction with the exogenous additive was placed in the lower tank, and the middle tank was an empty cavity without any substance. The upper and middle tanks were separated by a diaphragm to prevent the exogenous additive in the upper tank from entering the middle tank. The middle and lower tanks were separated by pretreated tobacco sheets. The control group membrane mixing tank was the same as the experimental group except that the solvent added to the upper tank was the same as the solvent used to prepare the exogenous additive solution. (3) Application of exogenous additives: The experimental group and the control group film mixing tanks are placed into the inner tank of the calorimeter. After the temperature of the film mixing tank set by the calorimeter reaches the set temperature, the membrane between the upper tank and the middle tank of the experimental group and the control group film mixing tank is punctured at the same time, so that the exogenous additives enter the middle tank and mix with the tobacco. (4) Heat detection: A calorimeter is used to detect and record the heat changes throughout the entire process of the external additives being applied to the tobacco sheet, penetrating into the tobacco sheet, and reacting with the reactants in the lower tank after passing through the tobacco sheet; (5) Calculation of permeation rate: Based on the time t of the exogenous additive permeating the tobacco leaf recorded by the calorimeter, as well as the diameter d and thickness h of the tobacco leaf, the permeation rate v of the exogenous additive in the tobacco leaf is calculated. The specific calculation formula is as follows:

2. The method of determining the rate of penetration of a tobacco exogenous additive in a tobacco leaf according to claim 1, wherein: In step (1), the tobacco leaves are placed in a constant temperature and humidity environment to balance the moisture content; the balanced tobacco leaf samples are cut into tobacco sheets with a diameter d of 8.27-11.00 mm using a punch; and the thickness h of the samples is measured using a thickness gauge.

3. The method of determining the rate of penetration of a tobacco exogenous additive in a tobacco leaf of claim 1, wherein: The specific structure of the thin film mixing tank in step (2) is as follows: it includes a base and a sleeve fitted on the base. In the sleeve, from bottom to top, there are a test smoke sheet fixing seat, a test smoke sheet fixing ring, an intermediate seat, a diaphragm fixing ring, and a diaphragm fixing seat. A test smoke sheet is sandwiched between the test smoke sheet fixing seat and the test smoke sheet fixing ring, and a diaphragm is sandwiched between the diaphragm fixing ring and the diaphragm fixing seat. The mixing tank is divided into a lower tank, a middle tank, and an upper tank by the arrangement of the test smoke sheet and the diaphragm.

4. The method of determining the rate of penetration of a tobacco exogenous additive in a tobacco leaf according to claim 1 or 3, wherein: The diaphragm is a thin film that carries exogenous additives and does not react with them; the thin film can be a Teflon film.

5. The method of determining the rate of penetration of a tobacco exogenous additive in a tobacco leaf of claim 1, wherein: The exogenous additives added to the upper pool in step (2) can be biological enzyme preparations such as pectinase and cellulase, tobacco flavoring liquids such as glycerol and citric acid; the corresponding exothermic reaction substances can be substances rich in pectin such as pectin powder and fruit juice, substances rich in cellulose such as plant cell wall extract and vegetable juice, alkaline substances and other substances that can react with the flavoring liquid to release heat.

6. The method for determining the penetration rate of exogenous tobacco additives in tobacco leaves according to claim 1 or 5, characterized in that: The substance that can undergo an exothermic chemical reaction with the external additives, as mentioned in step (2), can be either solid or liquid.

7. The method for determining the penetration rate of exogenous tobacco additives in tobacco leaves according to claim 2, characterized in that: The constant temperature and humidity environment can be a dynamic moisture adsorption-desorption instrument, a constant temperature and humidity chamber, or a constant temperature and humidity room; the punch can be a simple manual tobacco leaf punch; and the thickness gauge can be a portable electronic tobacco leaf thickness measuring instrument.

8. The method for determining the penetration rate of exogenous tobacco additives in tobacco leaves according to claim 1, characterized in that: The calorimeter mentioned can be a C80 micro calorimeter.

9. The method for determining the penetration rate of exogenous tobacco additives in tobacco leaves according to claim 1, characterized in that: The tobacco leaf sample is a sheet-like material, which can be roasted tobacco leaves or tobacco flakes. When it is roasted tobacco leaves, it can be placed on a petri dish and soaked with keratinase to dissolve the cuticle layer of the tobacco leaf epidermis to accelerate penetration.

10. The method for determining the penetration rate of exogenous tobacco additives in tobacco leaves according to claim 1, characterized in that: The measurement method and principle of the time t for the entire process of exogenous additives penetrating tobacco leaves, as recorded by the calorimeter, are as follows: When the exogenous additive solution comes into contact with the tobacco leaves, it will generate heat fluctuations, which will appear as a leading edge on the heat flow diagram. After the exogenous additive solution has penetrated the tobacco leaves, it will undergo an exothermic reaction with the substances in the lower pool, which will appear as a trailing edge on the heat flow diagram. The time between the leading edge and the trailing edge is the time taken for the exogenous additive solution to penetrate the tobacco leaves.

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