A grooming food formulation for improving the smoothness of cat fur

Abstract

The product belongs to the field of animal feed, and particularly relates to a hair conditioning food formula for improving the smoothness of cat hair. The formula comprises a targeted repair component and a biomimetic sebum reconstruction component; the targeted repair component comprises specific small-molecule bioactive peptides rich in cysteine; the biomimetic sebum reconstruction component comprises biomimetic sebum microcapsule liposomes, the core material of which contains ceramide, phytosterol and squalene, and the surface is modified with a lymphatic targeting ligand. The formula solves the problems of loss of sebum film adhesion and short smoothness maintenance period, cross-linking repair of damaged hair scales by bioactive peptides, targeted delivery of liposomes through lymph and spreading on the hair shaft surface to form a continuous sebum film arranged in a specific direction, reduction of the static coefficient of friction of hair, and reduction of the water loss rate.

Description

Technical Field

[0001] This product belongs to the field of animal feed, specifically involving a coat-enhancing food formula that improves the smoothness of a cat's fur. Background Technology

[0002] Current cat food formulas for improving coat smoothness typically involve directly adding crude fats or essential fatty acids, such as fish oil and flaxseed oil. After entering the cat's digestive tract, these fats are emulsified by bile and hydrolyzed by pancreatic lipases, forming fatty acids and monoglycerides. These fatty acids then pass through the intestinal mucosal epithelial cells and enter the lymphatic system or portal circulation. Upon reaching the sebaceous glands, these lipids are secreted onto the hair shaft surface, attempting to fill the gaps between hair cuticles through physical coverage, reducing the coefficient of friction, and thus achieving smoothness.

[0003] In the above-mentioned solutions, the directly added free fatty acids or triglycerides lack a guiding mechanism for directional arrangement and attachment during metabolism in the body. As a result, the lipids cannot form a regular and continuous hydrophobic barrier on the hair shaft surface after being secreted by the sebaceous glands. The lipids mostly exist in the form of discrete droplets, which are easily shed due to friction from the cat's daily activities. At the same time, the existing formula does not provide structural repair for the microscopic damaged areas of the hair cuticle. The edges of the damaged hair cuticles are raised, resulting in a rough surface. Even if there is lipid coverage, it cannot achieve adhesion, causing the core technical problems of loss of sebum film adhesion and short smoothness maintenance period. Summary of the Invention

[0004] In view of the shortcomings of existing technologies, such as the inability of lipids to form a regular and continuous hydrophobic barrier on the hair shaft surface, easy shedding, and failure to provide structural repair for microscopic damaged areas of the hair cuticle, resulting in loss of sebum film adhesion and short maintenance period of smoothness, this invention provides a coat-enhancing food formula to improve the smoothness of cat fur.

[0005] To address the aforementioned technical problems, this invention provides a coat-enhancing food formula for improving the smoothness of cat fur, comprising a targeted repair component and a biomimetic sebum reconstruction component; the targeted repair component includes a specific small molecule bioactive peptide rich in cysteine, wherein the N-terminus of the amino acid sequence of the specific small molecule bioactive peptide contains a disulfide bond domain; the biomimetic sebum reconstruction component includes biomimetic sebum microcapsules liposomes, wherein the capsule material of the biomimetic sebum microcapsules liposomes is composed of a phospholipid bilayer, and the core material of the biomimetic sebum microcapsules liposomes includes ceramide, phytosterol, and squalene, wherein the mass ratio of the ceramide, the phytosterol, and the squalene is 1:(0.5-2):(0.2-1); the surface of the biomimetic sebum microcapsules liposomes is modified with a lymphatic targeting ligand; the mass ratio of the specific small molecule bioactive peptide to the biomimetic sebum microcapsules liposomes is 1:(2-5).

[0006] This formula works through a synergistic mechanism of targeted repair and biomimetic sebum reconstruction. Cysteine-rich, specific small-molecule bioactive peptides, through their N-terminal disulfide-binding domain, specifically recognize and bind to keratin disulfide bond break sites exposed in damaged areas of the hair cuticle. New disulfide bonds are formed through a thiol-disulfide bond exchange reaction, achieving structural repair of the hair cuticle and allowing the raised cuticles to reattach to the hair shaft surface. Simultaneously, biomimetic sebum microcapsules modified with lymphatic-targeting ligands can be specifically taken up by the intestinal lymphatic system and accumulated in the sebaceous glands via lymphatic circulation, avoiding first-pass metabolic loss through the portal venous system. When the liposomes are secreted onto the hair shaft surface by the sebaceous glands, ceramides, phytosterols, and squalene in their core material are released in a specific ratio, mimicking the composition and structure of natural cat sebum. This allows them to spread directionally on the repaired and smoothed hair shaft surface, forming a dense, continuous, sebum-like hydrophobic barrier, effectively reducing the static friction coefficient of the hair and minimizing moisture loss.

[0007] Furthermore, in the above technical solution, the N-terminus of the specific small molecule bioactive peptide is modified with a cluster of thiol alanine residues, which provides free thiol groups; the targeted repair component also includes a zinc ion complex, in which zinc ions form a coordination cross-linking network with the free thiol groups of the specific small molecule bioactive peptide and the carboxyl groups of the keratin side chain; the zinc ion complex is zinc lactate or zinc gluconate.

[0008] In practice, the N-terminal thiol alanine residue cluster provides multiple highly reactive free thiol groups, significantly enhancing the binding ability of bioactive peptides to keratin. Zinc ions, as divalent metal ions, can simultaneously form coordination bonds with the free thiol groups of bioactive peptides and the carboxyl groups of keratin side chains, constructing a three-dimensional cross-linked network structure. This significantly improves the mechanical strength and abrasion resistance of the repair layer, prolonging the duration of the repair effect. Zinc lactate and zinc gluconate have good biocompatibility and intestinal absorption, enabling the stable release of zinc ions in vivo to participate in coordination reactions.

[0009] Furthermore, in the above technical solution, the phospholipid bilayer of the biomimetic sebaceous microcapsule liposome comprises hydrogenated soybean lecithin and cholesterol, wherein the molar ratio of hydrogenated soybean lecithin to cholesterol is (3-5):1; the surface of the phospholipid bilayer is also coated with a chitosan-hyaluronic acid copolymer coating, wherein the chitosan-hyaluronic acid copolymer coating is electrostatically adsorbed onto the outer surface of the phospholipid bilayer; the mass ratio of chitosan to hyaluronic acid is (2-4):1.

[0010] In practice, the phospholipid bilayer composed of hydrogenated soybean lecithin and cholesterol in a specific molar ratio exhibits suitable fluidity and membrane stability, effectively encapsulating the core material components and preventing leakage during storage. Chitosan-hyaluronic acid copolymers adsorb onto the negatively charged phospholipid bilayer surface through electrostatic interactions, forming a double-layer protective structure. This enhances the liposomes' resistance to degradation in the acidic environment of the stomach, while simultaneously increasing their adhesion to the intestinal mucosa, prolonging retention time, and promoting absorption.

[0011] Furthermore, in the above technical solution, the ceramide in the core material is ceramide 3 or ceramide 6, and the phytosterol is sitosterol or rapeseed sterol; the core material also contains essential fatty acid microdroplets, which are composed of linolenic acid and arachidonic acid in a mass ratio of 1:(0.8-1.5); the essential fatty acid microdroplets are dispersed in the squalene continuous phase to form an oil-in-oil emulsion droplet structure, which is located inside the biomimetic sebaceous microcapsule liposome.

[0012] In practice, ceramide 3 and ceramide 6 are the main components of lipids in the stratum corneum of cat skin, effectively repairing the skin barrier function. Sitosterol and brassicosterol have structures similar to animal sterols, exhibiting good biocompatibility and skin permeability. The oil-in-oil emulsion structure formed by essential fatty acid microdroplets dispersed in a squalene continuous phase enables the slow release of essential fatty acids, continuously providing the sebaceous glands with raw materials for sebum synthesis and prolonging the duration of the coat-enhancing effect.

[0013] Furthermore, in the above technical solution, the lymphatic targeting ligand is a mannose derivative modified with long-chain fatty acids, wherein the carbon chain length of the long-chain fatty acid is C16-C22; the mannose derivative modified with long-chain fatty acids is covalently linked to the hydrophilic end of the phospholipid bilayer; the mannose residues are exposed on the outermost surface of the biomimetic sebaceous microcapsule liposome, and the long-chain fatty acid is inserted into the hydrophobic layer of the phospholipid bilayer.

[0014] In practice, endothelial cells of the intestinal lymphatic system highly express mannose receptors, which can specifically recognize and bind to mannose residues exposed on the surface of liposomes, mediating liposome endocytosis and lymphatic transport. The hydrophobic tails of long-chain fatty acids are inserted into the hydrophobic regions of the phospholipid bilayer, which can stably anchor mannose derivatives to the surface of liposomes, preventing them from detaching during in vivo circulation and ensuring the stability of the targeting effect.

[0015] Furthermore, in the above technical solution, the specific small molecule bioactive peptide is covalently linked to the outer surface of the biomimetic sebum microcapsule liposome to form a core-shell coupled structure; the C-terminus of the specific small molecule bioactive peptide is connected to the phospholipid head on the surface of the biomimetic sebum microcapsule liposome via a maleimide-thiol click chemical bond; in the simulated intestinal fluid release system, the release rate of the specific small molecule bioactive peptide is greater than the release rate of the core material.

[0016] In practice, bioactive peptides are covalently linked to the surface of liposomes via a maleimide-thiol click chemistry reaction, forming a stable core-shell coupled structure that enables the co-delivery of two functional components. Because the bioactive peptides are located on the outer surface of the liposomes, they are preferentially released and absorbed in the intestinal fluid environment, reaching the hair follicle site ahead of time to repair the hair cuticle. Meanwhile, the lipid components in the core material are subsequently released and enriched in the sebaceous glands via lymphatic circulation, achieving a sequential effect of "repair first, then cover," ensuring that the lipids can form a continuous sebum film on the smooth hair shaft surface.

[0017] Furthermore, in the above technical solution, the coat-enhancing food formula also includes a micro-crosslinked sodium alginate framework material, which is formed by crosslinking sodium alginate with calcium ions; the targeted repair component and the biomimetic sebum reconstruction component are uniformly dispersed in the three-dimensional network of the micro-crosslinked sodium alginate framework material; the surface of the micro-crosslinked sodium alginate framework material is also embedded with an enteric polymer coating layer, which is a Eutec L-type or S-type polymethacrylate.

[0018] In practice, the micro-crosslinked sodium alginate framework material possesses a porous three-dimensional network structure, which can uniformly disperse and immobilize the targeted repair components and biomimetic sebum reconstruction components, preventing phase separation of the components during storage. The enteric polymer coating layer is insoluble in the gastric acid environment, protecting the internal components from degradation by gastric acid and pepsin; upon reaching the intestinal pH environment, the coating layer rapidly dissolves, releasing the sodium alginate framework material. The framework material gradually swells and degrades in the intestinal fluid, achieving slow release and continuous delivery of functional components.

[0019] Furthermore, in the above technical solution, the phytosterol is modified with succinic anhydride to form phytosterol succinate, and the free carboxyl group of the phytosterol succinate forms a salt structure with the divalent metal ion; the hydrophobic tail of the phytosterol succinate is inserted into the squalene continuous phase, and the hydrophilic head of the phytosterol succinate faces the interface region of the phospholipid bilayer; the divalent metal ion is a magnesium ion or a calcium ion, and the mass ratio of the phytosterol succinate to the squalene is 1:(3-6).

[0020] In practice, phytosterols are modified with succinic anhydride to introduce hydrophilic carboxyl groups, forming an amphiphilic molecular structure. The hydrophobic sterol tail can insert into the squalene continuous phase, while the hydrophilic carboxyl head faces the interface region of the phospholipid bilayer. This directional arrangement significantly enhances the membrane stability of liposomes. After forming a salt structure with divalent metal ions, the intermolecular interactions are further strengthened. When liposomes are secreted onto the hair shaft surface, they promote the orderly arrangement and spreading of lipid molecules, improving the skin's resistance to friction and shedding.

[0021] Furthermore, in the above technical solution, the specific small molecule bioactive peptide is obtained by double enzymatic hydrolysis of feather keratin with papain and neutral protease; the molecular weight distribution range of the specific small molecule bioactive peptide is 500-1500 Da; the thiol alanine residue cluster is located at the 1st-3rd position of the N-terminus of the specific small molecule bioactive peptide; the molar ratio of the zinc ion complex to the free thiol group of the specific small molecule bioactive peptide is 1:(2-4).

[0022] In practice, papain and neutral protease are used to hydrolyze feather keratin, specifically cleaving the peptide bonds of keratin to produce small peptides rich in cysteine. These peptides, with molecular weights ranging from 500 to 1500 Da, exhibit good intestinal absorption and bioactivity. The thiol-alanine residue cluster located at positions 1-3 of the N-terminus maximizes the exposure of free thiol groups, improving the binding efficiency with keratin and zinc ions. Zinc ions coordinate with free thiol groups at a molar ratio of 1:(2-4), forming a stable three-dimensional cross-linked network structure while avoiding the biotoxicity caused by excessive zinc ions.

[0023] Furthermore, in the above technical solution, the chitosan-hyaluronic acid copolymer coating is cross-linked and cured using genipin; the cross-linking sites of the genipin are located between the amino groups of the chitosan and the carboxyl groups of the hyaluronic acid; a polyvinylpyrrolidone protective layer is also adsorbed on the outer side of the chitosan-hyaluronic acid copolymer coating, the polyvinylpyrrolidone having a weight-average molecular weight of 10,000-30,000; the mass ratio of the polyvinylpyrrolidone protective layer to the chitosan-hyaluronic acid copolymer coating is 1:(5-8).

[0024] In practice, genipin, as a natural cross-linking agent, can react with the amino groups of chitosan and the carboxyl groups of hyaluronic acid to form a stable covalent cross-linked structure, significantly improving the mechanical strength and enzymatic resistance of the copolymer coating. Polyvinylpyrrolidone is adsorbed onto the outer side of the copolymer coating through hydrogen bonding and electrostatic interactions, which can further enhance the stability of liposomes in the gastric acid environment, while reducing their non-specific adsorption in the gastrointestinal tract and improving targeted delivery efficiency.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This technical solution provides a synergistic system of specific small-molecule bioactive peptides rich in cysteine ​​and biomimetic sebum microcapsule liposomes, solving the problems of loss of sebum film adhesion and short maintenance period of smoothness. The free sulfhydryl groups of the specific small-molecule bioactive peptides bind to the keratin side chain groups, forming a cross-linked repair network in the damaged area of ​​the hair cuticle, allowing the raised hair cuticles to adhere to the hair shaft and eliminating physical friction obstacles; the biomimetic sebum microcapsule liposomes provide a combination of ceramides, phytosterols and squalene, and are modified with lymphatic targeting ligands on the surface to guide lipids to accumulate in the sebaceous glands via lymphatic circulation. After secretion, they spread on the repaired and smoothed hair shaft surface, forming a dense and continuous directional sebum film structure, which reduces the static friction coefficient of the hair and decreases the moisture loss rate.

[0026] 2. This technical solution further strengthens the ability to maintain smoothness through specific structural design. Zinc ion complexes form a coordination cross-linking network with free thiol groups and keratin, enhancing the binding strength of the repair layer; the chitosan-hyaluronic acid copolymer coating and polyvinylpyrrolidone protective layer ensure the structural integrity of the microcapsules in the gastric acid environment and release them in intestinal fluid; the enteric polymer coating layer and the micro-crosslinked sodium alginate framework material control the release sequence of the targeted repair components and the core material, so that the repair peptides are preferentially released and bound to the hair cuticles, followed by the release of lipids for coverage; phytosterols are modified with succinic anhydride to phytosterol succinate and form a salt structure with divalent metal ions, so that the hydrophobic tail is anchored to the squalene continuous phase and the hydrophilic head faces the phospholipid interface, improving the spreading stability and anti-friction shedding ability of the liposomes after being secreted into the hair shaft. Detailed Implementation

[0027] The present invention will be further described in detail below with reference to embodiments. Those skilled in the art can reproduce the technical solution of the present invention and achieve its claimed technical effects based on the content disclosed in this specification. It should be noted that the following embodiments are only used to explain the present invention and are not intended to limit the scope of protection of the present invention. Any non-substantial improvements and adjustments made based on the core concept of the present invention should fall within the scope of protection of the present invention.

[0028] Example 1: This example provides a coat-enhancing food formula to improve the smoothness of cat fur. Its specific composition is as follows: the mass ratio of specific small molecule bioactive peptides to biomimetic sebum microcapsules liposomes is 1:3; the mass ratio of ceramide 3, phytosterol calcium succinate, and squalene in the core material of the biomimetic sebum microcapsules is 1:1:0.5; the mass ratio of linolenic acid to arachidonic acid in the essential fatty acid microdroplets is 1:1; the molar ratio of hydrogenated soybean lecithin to cholesterol in the phospholipid bilayer is 4:1; chitosan and... The mass ratio of hyaluronic acid is 3:1; the lymphatic targeting ligand is a C18 stearic acid-modified mannose derivative; the zinc ion complex is zinc gluconate, and the molar ratio of zinc ions to free thiol groups of specific small molecule bioactive peptides is 1:3; the mass ratio of phytosterol calcium succinate to squalene is 1:4; the mass ratio of polyvinylpyrrolidone (weight average molecular weight 20,000) to chitosan-hyaluronic acid copolymer coating is 1:6; and the enteric polymer coating layer is Utec L100 type polymethyl methacrylate.

[0029] Its preparation method includes the following steps: Preparation of specific small molecule bioactive peptides: Feather keratin powder was added to 0.1 mol / L phosphate buffer (pH 7.5) to prepare a 10% (w / v) suspension, which was then heated to 50°C and kept at that temperature. Papain (10000 U / g) and neutral protease (8000 U / g) were added sequentially, at amounts of 2% and 1.5% of the keratin dry weight, respectively. The mixture was stirred at 150 rpm for 4 h for hydrolysis. After hydrolysis, the mixture was rapidly heated to 95°C and kept at that temperature for 10 min to inactivate the enzymes. After cooling to room temperature, the mixture was centrifuged at 4000 rpm for 15 min, and the supernatant was collected. The supernatant was separated by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 1500 Da. The permeate was collected and freeze-dried at -50°C for 24 h to obtain specific small molecule bioactive peptides. The molecular weight distribution range was 500-1500 Da as determined by high performance gel permeation chromatography; the free thiol content was 120 μmol / g as determined by the Ellman method; and the N-terminal amino acid sequence showed that the N-terminal 1-3 positions were cysteine ​​residue clusters.

[0030] Preparation of phytosterol calcium succinate: Sitosterol and succinic anhydride were added to a reaction vessel at a molar ratio of 1:1.2. Pyridine was added as a catalyst (5% of the mass of phytosterol). Nitrogen gas was introduced for protection, and the mixture was stirred at 80°C for 6 hours. After the reaction, the mixture was cooled to room temperature, and 5 times its volume of anhydrous ethanol was added to precipitate the product. The precipitate was filtered, and the filter cake was washed three times with anhydrous ethanol and dried under vacuum at 40°C for 12 hours to obtain phytosterol succinate. Phytosterol succinate was dissolved in anhydrous ethanol to prepare a 5% (w / v) solution. An equimolar amount of calcium chloride was added, and the mixture was stirred at room temperature for 2 hours. The ethanol was removed by rotary evaporation to obtain phytosterol calcium succinate.

[0031] Preparation of biomimetic sebum microcapsule liposomes: Oil phase preparation: Hydrogenated soybean lecithin, cholesterol (molar ratio 4:1) and stearic acid-modified mannose derivative (5% of the total mass of phospholipids) were dissolved in a chloroform-methanol mixed solvent (volume ratio 2:1), transferred to a rotary evaporator flask, and the organic solvent was removed by rotary evaporation under reduced pressure at 40℃ and 0.08MPa, forming a uniform and transparent lipid film on the flask wall.

[0032] Core material preparation: Ceramide 3, phytosterol calcium succinate, and squalene were mixed in a mass ratio of 1:1:0.5 and heated to 50°C while stirring until completely dissolved to form a continuous phase. Linolenic acid and arachidonic acid were mixed in a mass ratio of 1:1 to prepare an essential fatty acid mixture, which was then slowly added dropwise to the above continuous phase. The mixture was stirred at a high speed of 10,000 r / min for 5 min to form a stable oil-in-oil emulsion droplet structure.

[0033] Hydration and ultrasonic dispersion: The above oil-in-oil emulsion droplet structure was added to a pH 7.4 phosphate buffer solution preheated to 40°C to achieve a total lipid concentration of 5% (w / v), and hydrated at 40°C for 30 min. Subsequently, ultrasonic treatment was performed using a probe sonicator at a power of 200W, with a working time of 3 s and a pause of 2 s, for a total ultrasonic time of 10 min, to obtain a liposome suspension with uniform particle size.

[0034] Preparation of chitosan-hyaluronic acid copolymer coating: Chitosan with a degree of deacetylation of 85% was dissolved in 1% (v / v) acetic acid solution to prepare a 2% (w / v) chitosan solution; hyaluronic acid was dissolved in deionized water to prepare a 1% (w / v) hyaluronic acid solution. The two solutions were mixed at a chitosan to hyaluronic acid mass ratio of 3:1 and stirred for 30 min to obtain a copolymer solution. The above liposome suspension was slowly added dropwise to the copolymer solution (liposome to copolymer mass ratio of 1:1), and stirred at room temperature for 30 min to allow the copolymer to be electrostatically adsorbed onto the liposome surface. Then, genipin (0.5% of the total copolymer mass) was added, and crosslinking and curing were carried out at room temperature for 2 h.

[0035] Adsorption of the polyvinylpyrrolidone (PVP) protective layer: A 5% (w / v) solution of PPVP with a weight-average molecular weight of 20,000 was prepared by dissolving PPVP in deionized water. The liposome suspension coated with the copolymer was slowly added dropwise to the PPVP solution (copolymer to PPVP mass ratio of 6:1), and stirred at room temperature for 2 hours. The liposomes were collected by centrifugation at 12,000 rpm for 20 minutes, washed three times with pH 7.4 phosphate buffer, and resuspended in the same buffer.

[0036] Preparation of the core-shell coupled structure: N-hydroxysuccinimide (NHS) and 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) were added to the above liposome suspension at a molar ratio of 1:1.2, and the mixture was activated at room temperature for 30 min. Subsequently, a specific small-molecule bioactive peptide (liposome to peptide mass ratio of 3:1) was added, and the mixture was stirred at room temperature for 4 h to allow the C-terminal thiol group of the peptide to undergo a click chemical reaction with the maleimide group of the phospholipid head on the liposome surface, forming a covalent link. After the reaction, the product was collected by centrifugation at 12000 r / min for 20 min, and washed three times with phosphate buffer to obtain the core-shell coupled structure.

[0037] Preparation of the micro-crosslinked sodium alginate framework: Sodium alginate was dissolved in deionized water to prepare a 3% (w / v) solution. The core-shell coupled structure was dispersed in the sodium alginate solution and stirred until homogeneous, resulting in a final concentration of 10% (w / v). The mixture was added dropwise to a 1% (w / v) calcium chloride solution using a peristaltic pump at a flow rate of 5 mL / min, and cured at room temperature for 30 min to form micro-crosslinked sodium alginate microspheres. The microspheres were collected by filtration, washed three times with deionized water, and then freeze-dried under vacuum at -50℃ for 24 h.

[0038] Enteric coating: Eutec L100 was dissolved in 95% (v / v) ethanol to prepare a 10% (w / v) coating solution. The above sodium alginate microspheres were placed in a fluidized bed coating machine, and the coating solution was sprayed in. The inlet air temperature was controlled at 40℃ and the outlet air temperature at 30℃, and the coating weight gain was 10% (w / w). After coating, the microspheres were dried at 40℃ for 2 hours to obtain the final wool-enhancing food formulation granules.

[0039] Example 2: The only difference between this example and Example 1 is that the mass ratio of the specific small molecule bioactive peptide to the biomimetic sebum microcapsule liposome is 1:2. The rest of the formulation and preparation method are the same as in Example 1.

[0040] Example 3: The only difference between this example and Example 1 is that the mass ratio of the specific small molecule bioactive peptide to the biomimetic sebum microcapsule liposome is 1:5. The rest of the formulation and preparation method are the same as in Example 1.

[0041] Example 4: The only difference between this example and Example 1 is that ceramide 3 in the core material is replaced with ceramide 6, and sitosterol is replaced with rapeseed sterol. The rest of the formulation and preparation method are the same as in Example 1.

[0042] Example 5: The only difference between this example and Example 1 is that the zinc ion complex is replaced by zinc lactate instead of zinc gluconate. The rest of the formulation and preparation method are the same as in Example 1.

[0043] Example 6: The only difference between this example and Example 1 is that the molar ratio of hydrogenated soybean lecithin to cholesterol is 3:1. The rest of the formulation and preparation method are the same as in Example 1.

[0044] Example 7: The only difference between this example and Example 1 is that the molar ratio of hydrogenated soybean lecithin to cholesterol is 5:1. The rest of the formulation and preparation method are the same as in Example 1.

[0045] Example 8: The only difference between this example and Example 1 is that the enteric polymer coating layer is replaced by Eutectic L100 type with Eutectic S100 type. The rest of the formulation and preparation method are the same as in Example 1.

[0046] Example 9: The only difference between this example and Example 1 is that the lymphatic targeting ligand is a C16 palmitic acid modified mannose derivative, while the rest of the formulation and preparation method are the same as in Example 1.

[0047] Example 10: The only difference between this example and Example 1 is that the double-enzyme hydrolysis temperature in the preparation of the specific small molecule bioactive peptide is changed from 50℃ to 45℃. The rest of the formulation and preparation method are the same as in Example 1.

[0048] Example 11: The only difference between this example and Example 1 is that the lipid film hydration temperature is changed from 40℃ to 35℃ during the preparation of the biomimetic sebum microcapsule liposomes. The rest of the formulation and preparation method are the same as in Example 1.

[0049] Example 12: The only difference between this example and Example 1 is that the crosslinking time of genipin is changed from 2h to 1.5h in the preparation process of the chitosan-hyaluronic acid copolymer coating. The rest of the formulation and preparation method are the same as in Example 1.

[0050] Comparative Example 1: The only difference between this comparative example and Example 1 is that the formulation does not contain specific small molecule bioactive peptides, while the rest of the formulation composition and preparation method are the same as in Example 1.

[0051] Comparative Example 2: The traditional coat-enhancing food formula described in the background section was adopted, specifically composed of: 20% fish oil, 15% flaxseed oil, 40% chicken meal, 20% corn starch, 1% vitamin E, and 4% mineral premix. Following conventional feed preparation methods, the components were mixed evenly, granulated using a twin-screw extruder, and dried to obtain the finished product.

[0052] Comparative Example 3: The only difference between this comparative example and Example 1 is that the mass ratio of ceramide 3, phytosterol calcium succinate, and squalene in the biomimetic sebum microcapsule liposome core material is 1:3:0.1. The rest of the formulation and preparation method are the same as in Example 1.

[0053] Comparative Example 4: The only difference between this comparative example and Example 1 is that the core-shell coupled structure preparation step is omitted, and the specific small molecule bioactive peptide and the biomimetic sebaceous microcapsule liposome are physically mixed at a mass ratio of 1:3. The rest of the formulation and preparation method are the same as in Example 1.

[0054] Test method: Static friction coefficient test of hair: Twenty healthy adult short-haired domestic cats were selected. Hair from the same area on the back was shaved and cut into uniform 2cm lengths. The hair was ultrasonically cleaned three times with anhydrous ethanol for 5 minutes each time, and then air-dried. The hair was then fixed parallel to a glass slide to form a uniformly dense hair layer. Using a friction coefficient tester (MFT-5000), at 25℃ and 50% relative humidity, the static friction coefficient of the hair surface was measured at a sliding speed of 5mm / s. Each sample was tested five times, and the average value was taken.

[0055] Hair moisture loss rate test: The treated hair was placed in a constant temperature and humidity chamber (25℃, 50% relative humidity) for equilibration for 24 hours and accurately weighed (recorded as W0). It was then transferred to a desiccator (25℃, 10% relative humidity) for 24 hours and accurately weighed again (recorded as W1). The moisture loss rate was calculated using the formula: Moisture loss rate (%) = (W0 - W1) / W0 × 100%. Each sample was tested 5 times, and the average value was taken.

[0056] Sebum film lipid residue rate test: The treated hair was rubbed 100 times with a standard abrasion instrument at a load of 500g and a frequency of 100 times / min. Then, the lipids on the hair surface were extracted with hexane by ultrasound for 30 min, and the extraction was repeated 3 times. The extracts were combined, rotary evaporated to dryness, and the lipid content was determined by gas chromatography (denoted as C1). At the same time, the lipid content of the unrubbed hair was determined (denoted as C0). The lipid residue rate was calculated by the formula: lipid residue rate (%) = C1 / C0 × 100%. Each sample was tested 5 times, and the average value was taken.

[0057] Smoothness maintenance time test: 160 healthy adult short-haired domestic cats were randomly divided into 16 groups of 10 cats each. Each group was fed the samples of Examples 1-12 and Comparative Examples 1-4, respectively, at a daily dose of 10g / cat for 7 consecutive days. Starting from the day after the end of feeding, hair samples from the same area on the back of each cat were collected weekly to test the static friction coefficient. When the static friction coefficient rose above 0.20, the smoothness maintenance time was recorded, and the average value of each group was taken.

[0058] Test results: Table 1. Test results of the hair-beautifying effect of each embodiment and comparative example.

[0059] Results analysis: Compared with Comparative Example 2 (Prior Art), the static friction coefficient of all embodiments was reduced by 40%-52%, the moisture loss rate was reduced by 46%-57%, the lipid residue rate was increased by 95%-112%, and the smoothness maintenance time was extended by 1.8-2.8 times. This fully demonstrates that the present invention effectively solves the core defects of the prior art, such as the inability of lipids to form a continuous hydrophobic barrier, easy shedding, and failure to repair damaged hair cuticles, through the synergistic mechanism of targeted repair and biomimetic sebum reconstruction, achieving unexpected technical effects.

[0060] Comparative Example 1, lacking specific small-molecule bioactive peptides, showed significant deterioration in all its indicators, with a static friction coefficient as high as 0.28, a lipid residue rate of only 35%, and a maintenance time of only 7 days. This indicates that the targeted repair component is the core foundation of this invention. Only by structurally repairing the damaged areas of the hair cuticle through specific small-molecule bioactive peptides, allowing the raised cuticles to reattach to the hair shaft, can a smooth surface be provided for subsequent lipid spreading, forming a dense and continuous sebum film.

[0061] Comparative Example 3 deviated from the proportions of the core components defined in this invention, resulting in a significant decrease in effectiveness, with a static friction coefficient of 0.22 and a lipid residue rate of 51%. This indicates that the specific ratio of ceramides, phytosterols, and squalene is crucial for mimicking the structure of natural cat sebum. Deviating from this ratio disrupts the directional arrangement of lipid molecules, preventing the formation of a stable sebum-like film structure, thereby reducing the adhesion and friction resistance of the sebum film.

[0062] Comparative Example 4 omitted the covalent linking step, resulting in only physical mixing of the bioactive peptide and liposomes, which significantly reduced its effectiveness, with a duration of only 15 days. This demonstrates that the core-shell coupling structure can achieve co-delivery and sequential release of the two functional components, ensuring a "repair first, cover later" action sequence. Without covalent linking, the bioactive peptide and liposome would experience asynchronous release and absorption in vivo, failing to form an effective synergistic effect.

[0063] Examples 2-9 adjusted the formulation parameters within the scope defined by this invention, and Examples 10-12 adjusted the preparation method parameters. All indicators remained at a high level, with minimal difference from the best example 1. This demonstrates that the technical solution of this invention has good robustness and universality, and can stably achieve the expected technical effects within the defined scope.

Claims

1. A formula for a coat-enhancing food that improves the smoothness of a cat's fur, characterized in that, Includes targeted repair components and biomimetic sebum reconstruction components; The targeted repair component includes a specific small molecule bioactive peptide rich in cysteine, wherein the N-terminus of the amino acid sequence of the specific small molecule bioactive peptide contains a disulfide bond binding domain. The biomimetic sebum reconstruction component includes biomimetic sebum microcapsule liposomes. The capsule material of the biomimetic sebum microcapsule liposomes is composed of a phospholipid bilayer. The core material of the biomimetic sebum microcapsule liposomes includes ceramide, phytosterol and squalene. The mass ratio of the ceramide, the phytosterol and the squalene is 1:(0.5-2):(0.2-1). The surface of the biomimetic sebaceous microcapsule liposomes is modified with a lymphatic targeting ligand. The mass ratio of the specific small molecule bioactive peptide to the biomimetic sebaceous microcapsule liposome is 1:(2-5).

2. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The N-terminus of the specific small molecule bioactive peptide is modified with a cluster of thiol alanine residues, which provides free thiol groups. The targeted repair component also includes a zinc ion complex, wherein the zinc ions in the zinc ion complex form a coordination cross-linking network with the free thiol groups of the specific small molecule bioactive peptide and the carboxyl groups of the keratin side chain; The zinc ion complex is zinc lactate or zinc gluconate.

3. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The phospholipid bilayer of the biomimetic sebaceous microcapsule liposome comprises hydrogenated soybean lecithin and cholesterol, wherein the molar ratio of hydrogenated soybean lecithin to cholesterol is (3-5):

1. The surface of the phospholipid bilayer is further coated with a chitosan-hyaluronic acid copolymer coating, which is electrostatically adsorbed onto the outer surface of the phospholipid bilayer. The mass ratio of chitosan to hyaluronic acid is (2-4):

1.

4. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The ceramide in the core material is ceramide 3 or ceramide 6, and the phytosterol is sitosterol or rapeseed sterol. The core material also contains essential fatty acid droplets, which are composed of linolenic acid and arachidonic acid in a mass ratio of 1:(0.8-1.5). The essential fatty acid microdroplets are dispersed in the squalene continuous phase to form an oil-in-oil emulsion droplet structure, which is located inside the biomimetic sebaceous microcapsule liposome.

5. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The lymphatic targeting ligand is a mannose derivative modified with long-chain fatty acids, wherein the carbon chain length of the long-chain fatty acids is C16-C22. The long-chain fatty acid-modified mannose derivative is covalently linked to the hydrophilic end of the phospholipid bilayer; The mannose residues in the mannose derivative are exposed on the outermost surface of the biomimetic sebaceous microcapsule liposome, and the long-chain fatty acid is inserted into the hydrophobic layer of the phospholipid bilayer.

6. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The specific small molecule bioactive peptide is covalently linked to the outer surface of the biomimetic sebaceous microcapsule liposome to form a core-shell coupled structure. The C-terminus of the specific small molecule bioactive peptide is connected to the phospholipid head on the surface of the biomimetic sebum microcapsule liposome via a maleimide-thiol click chemical bond. In the simulated intestinal fluid release system, the release rate of the specific small molecule bioactive peptide is greater than that of the core material.

7. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The formula for the hair-enhancing food also includes a micro-crosslinked sodium alginate framework material, which is formed by crosslinking sodium alginate with calcium ions. The targeted repair component and the biomimetic sebum reconstruction component are uniformly dispersed in the three-dimensional network of the micro-crosslinked sodium alginate framework material; The surface of the micro-crosslinked sodium alginate framework material is further embedded with an enteric polymer coating layer, which is a Utec L-type or S-type polymethacrylate.

8. The coat-enhancing food formula for improving the smoothness of cat fur according to claim 1, characterized in that, The phytosterol is modified with succinic anhydride to form phytosterol succinate, and the free carboxyl group of the phytosterol succinate forms a salt structure with divalent metal ions. The hydrophobic tail of the phytosterol succinate is inserted into the squalene continuous phase, and the hydrophilic head of the phytosterol succinate faces the interface region of the phospholipid bilayer. The divalent metal ion is a magnesium ion or a calcium ion, and the mass ratio of the phytosterol succinate to the squalene is 1:(3-6).

9. The formula for a coat-enhancing food that improves the smoothness of a cat's fur according to claim 2, characterized in that, The specific small molecule bioactive peptides are prepared by double enzymatic hydrolysis of feather keratin with papain and neutral protease. The molecular weight distribution range of the specific small molecule bioactive peptide is 500-1500 Da; The thiol alanine residue cluster is located at positions 1-3 of the N-terminus of the specific small molecule bioactive peptide; The molar ratio of the zinc ion complex to the free thiol group of the specific small molecule bioactive peptide is 1:(2-4).

10. The formula for a coat-enhancing food that improves the smoothness of a cat's fur according to claim 3, characterized in that, The chitosan-hyaluronic acid copolymer coating is cross-linked and cured using genipin. The cross-linking site of the genipin is located between the amino group of the chitosan and the carboxyl group of the hyaluronic acid; The outer side of the chitosan-hyaluronic acid copolymer coating is also adsorbed with a polyvinylpyrrolidone protective layer, wherein the weight-average molecular weight of the polyvinylpyrrolidone is 10,000-30,000. The mass ratio of the polyvinylpyrrolidone protective layer to the chitosan-hyaluronic acid copolymer coating is 1:(5-8).

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