Affinity short peptide ligands that specifically bind cd3 antibodies and uses thereof

By designing short peptide ligands with high specificity and high affinity targeting CD3ε antibodies, the stability, cost, and safety issues in the purification of TCE bispecific/polyclonal antibodies were resolved, achieving efficient and low-cost purification results.

CN122356239APending Publication Date: 2026-07-10BALINKE (LANZHOU) NEW MATERIALS CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BALINKE (LANZHOU) NEW MATERIALS CO LTD
Filing Date
2026-04-14
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing TCE bispecific/multispecific antibody purification technologies suffer from poor stability, high cost, high immunogenicity risk, insufficient specificity, and nonspecific binding issues. There is a lack of efficient short peptide ligands for recognizing CD3 binding arms and purification protocols.

Method used

A highly specific, high-affinity short peptide ligand targeting CD3ε antibody was designed, mimicking the N-terminal epitope of CD3ε, and covalently coupled to a solid-phase carrier for efficient purification of TCE bispecific/polyclonal antibodies, specifically binding to anti-CD3 antibodies or their antigen-binding fragments.

Benefits of technology

It achieves highly specific purification, improves purification specificity and recovery rate, enhances the ability to remove mismatches and impurities, reduces costs, improves packing stability and safety, and optimizes chromatographic purification efficiency.

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Abstract

This application discloses an affinity short peptide ligand and its application, wherein the affinity short peptide ligand can specifically bind to anti-CD3 antibodies or their antigen-binding fragments. This invention provides a binding short peptide with high specificity and high affinity for CD3ε antibodies (e.g., SP34), which, as a ligand for affinity chromatography packing materials, achieves efficient purification of TCE bispecific / polyclonal antibodies, filling a technological gap in this field.
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