A method for preparing clostridium welchii inhibitor by using hermetia illucens oil

Black soldier fly oil inhibitors were prepared using a low-temperature physical extraction process throughout the entire process, which solved the problem of activity loss caused by heat treatment and achieved a significant inhibitory effect on Clostridium perfringens while also enabling environmentally friendly production.

CN122397754APending Publication Date: 2026-07-17

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Filing Date
2026-04-23
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for extracting black soldier fly oil generally involve heat treatment, which leads to the degradation of its antibacterial active ingredients against Clostridium perfringens, and there is no effective extraction process to preserve its inhibitory effect on anaerobic pathogens.

Method used

A low-temperature physical extraction process was adopted, including low-temperature vibration crushing and low-temperature centrifugation, avoiding heat treatment, to prepare an insect oil inhibitor with Clostridium perfringens inhibitory activity.

Benefits of technology

It effectively retains the natural antibacterial active ingredients in insect oil, significantly inhibits the anaerobic bacterium Clostridium perfringens, and the preparation process is simple, environmentally friendly, and suitable for industrial application.

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Abstract

本发明公开了一种利用黑水虻虫油制备魏氏梭菌抑制剂的方法,属于生物提取及微生物抑制技术领域。该方法包括:取新鲜黑水虻幼虫清洗,与钢珠混合后进行震荡破碎处理;将破碎后的虫体匀浆在0‑10℃低温条件下离心分离;收集离心后形成的凝固层,即为具有魏氏梭菌抑制活性的虫油抑制剂,其在抑菌圈实验中抑菌圈直径≥12mm。整个制备过程不进行加热处理,有效保留了虫油中对魏氏梭菌敏感的天然热敏性抑菌活性成分。本发明首次发现并验证了低温提取黑水虻虫油对厌氧致病菌魏氏梭菌的显著抑制作用,解决了传统加热提取工艺导致抑菌活性丧失的问题。所得抑制剂可用于培养基添加、饲料添加或饮水添加,应用前景广阔。
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