Application of pear transcription factor pbrhb49 in regulating lignin synthesis in stone cells

By identifying and regulating the PbrHB49 gene in pear fruit, the unknown mechanism of stone cell formation in pear fruit was solved, and effective regulation of lignin synthesis was achieved, which improved fruit quality and provided gene resources for molecular breeding and industrial applications.

CN122405643APending Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2026-03-19
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The formation mechanism of stone cells in pear fruit is not fully understood in the current technology, especially the regulatory mechanism of the HD-Zip gene in lignin synthesis has not been reported, which leads to rough fruit texture and affects quality.

Method used

The PbrHB49 gene in pear fruit was isolated and identified. It belongs to the HB-HD-Zip family. By constructing recombinant expression vectors and gene silencing vectors, the synthesis of lignin in pear fruit stone cells was regulated, and the production of lignin was promoted or inhibited.

Benefits of technology

By transiently overexpressing or silencing the PbrHB49 gene, the accumulation process of stone cells and lignin in pear fruits was significantly altered, providing a theoretical basis for improving pear fruit quality and offering genetic resources for molecular breeding and reducing lignin content.

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Abstract

This invention discloses the application of the pear transcription factor PbrHB49 in regulating lignin synthesis in sclereids. A transcription factor gene, PbrHB49, isolated from 'Dangshan Crisp Pear', which promotes lignin production in sclereids, has its nucleotide sequence shown in SEQ ID No. 1. Through transient overexpression in pear fruit and gene silencing techniques, it was confirmed that PbrHB49 can alter the accumulation process of sclereids and lignin in the fruit. In transgenic pear pulp callus tissue and Arabidopsis inflorescence stems overexpressing PbrHB49, lignin content was significantly increased. Simultaneously, phloroglucinol hydrochloride staining, toluidine blue staining, potassium permanganate staining, and lignin autofluorescence signals in Arabidopsis stems were significantly enhanced. This demonstrates that the PbrHB49 gene participates in regulating the formation of sclereids and their component lignin in pear fruit. Furthermore, the PbrHB49 gene has the advantage of simultaneously regulating multiple genes, providing a more efficient approach for molecular breeding.
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