Application of soybean vacuolar sorting receptor protein Gmvsr3-10 encoding gene
By knocking out the soybean vacuole sorting receptor protein GmVSR3-10 gene using CRISPR/Cas9 gene editing technology, the problem of insufficient salt tolerance during soybean germination was solved, resulting in improved seed germination rate and germination potential, increased hypocotyl length, and enhanced salt tolerance of soybean.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- NANJING AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-06-09
- Publication Date
- 2026-07-17
AI Technical Summary
Under salt stress, soybean seed germination rate and germination potential decrease during the germination period, and the hypocotyl length becomes shorter, affecting seed germination and growth. Current technology lacks effective gene regulation methods to improve salt tolerance.
The soybean vacuolar sorting receptor protein encoding gene GmVSR3-10 was knocked out using CRISPR/Cas9 gene editing technology. The gene was then edited using specific sgRNA sequences (SEQ ID NO.7 and SEQ ID NO.8) to construct a CRISPR/Cas9 gene knockout vector, thereby improving the salt tolerance of soybeans during germination.
Knocking out the GmVSR3-10 gene increased the germination rate and germination potential of soybean seeds, lengthened the hypocotyl, significantly improved the salt tolerance of soybeans during germination, and enhanced the soybeans' ability to adapt to salt stress.
Smart Images
Figure CN122405723A_ABST