Carbon and nitrogen isotope dual-chamber labeling and control device and method for intercropping system

Abstract

The application provides a carbon-nitrogen isotope double-chamber labeling and control device and method for intercropping systems, which comprises a soil tank with a bottom separated by a removable partition, and double labeling chambers arranged above the soil tank, and the bottom of the labeling chambers is in airtight connection with the soil tank through a water seal structure. A reaction supply assembly, a sensor group and a cooperative closed-loop control module are arranged in each labeling chamber. The module synchronously collects the gas concentration, pressure difference, temperature and humidity and the state of the execution component of the double chambers, cooperatively determines the working condition based on the concentration difference, pressure difference relationship and concentration attenuation characteristics of the two chambers, distinguishes the plant normal absorption, supply deviation, sealing leakage and inter-chamber gas leakage state, and performs linkage compensation control on the supply rate, valve opening degree and fan parameters. The application realizes the continuous connection of the partitioned and isolated labeling of the aboveground parts of different plants and the interactive research of the underground root systems under the intercropping system, effectively suppresses the gas crosstalk, and improves the accuracy and stability of the isotope labeling.

Description

Technical Field

[0001] This invention relates to the field of isotope tracing and agricultural ecology research technology, and in particular to a carbon and nitrogen isotope dual-chamber labeling and control device and method for intercropping systems. Background Technology

[0002] Stable isotope tracing is an important tool in ecological research. By tracing the pathways of stable isotopes such as carbon and nitrogen, it is possible to gain in-depth insights into key scientific issues such as plant physiological and ecological processes, ecosystem material cycling, and energy flow. In the field of agricultural ecology, this technology is widely used to study plant water use efficiency, nutrient uptake and distribution, photosynthetic product translocation, and carbon and nitrogen cycling processes in soil-plant systems. Currently, the commonly used technique for carbon isotope labeling of plants is to use a sealed labeling box made of transparent PVC material. The procedure involves placing a certain concentration of sulfuric acid in a beaker inside the box, and then injecting Na2 through a rigid plastic tube. 13 CO3, the two react chemically to release CO3, 13 CO2 gas. Subsequently, the plants inside the enclosure absorb these enriched gases through photosynthesis. 13 The carbon dioxide of C makes 13 C isotopes are integrated into the organism to track the distribution dynamics of photosynthetic products in organs such as roots, stems, leaves, flowers, and seeds, and further to track their entire turnover path as they form with litter and enter the soil.

[0003] However, the aforementioned traditional labeling boxes have significant limitations, particularly in meeting the research needs of complex intercropping systems in modern agriculture. Firstly, traditional labeling boxes are typically designed for monoculture systems or single-labeling chambers, often only capable of labeling a single type of isotope on a single plant population at a time, making it difficult to simultaneously perform differentiated carbon and nitrogen isotope tracing on two different plants within the same intercropping system. Secondly, while existing solutions can perform closed labeling of the aboveground parts of plants, they often lack structural designs that accommodate subsequent research on root interactions, making it difficult to maintain continuous in-situ research after labeling. Thirdly, existing devices often employ independent monitoring and adjustment of parameters within each labeling chamber, typically relying on replenishment based on a single chamber's concentration threshold. This makes it difficult to comprehensively utilize the concentration difference, pressure difference, concentration decay characteristics, and actuator status between the two chambers to determine the cause of concentration changes within the labeling chamber. Consequently, it is difficult to distinguish between concentration changes caused by normal plant absorption and abnormal changes caused by supply deviations, water seal leaks, or inter-chamber cross-ventilation, making it challenging to simultaneously maintain the target environment and suppress gas cross-talk in parallel labeling scenarios within two chambers. Summary of the Invention

[0004] This invention discloses a carbon and nitrogen isotope dual-chamber labeling and control device and method for intercropping systems, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, a first aspect of the present invention provides a carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems, the device comprising: The soil box at the bottom and the marking assembly located above the soil box, the interior of which is divided into two independent root growth zones by a removable partition; The marking assembly includes a first marking chamber and a second marking chamber respectively corresponding to two root growth zones. The first marking chamber and the second marking chamber are respectively formed by a transparent marking chamber body located above the soil box. A sealing assembly is provided at the bottom of the transparent marking chamber body where it connects to the soil box to form an airtight connection. The first and second labeling chambers are respectively equipped with a reaction supply component, a fan, a gas concentration detection unit, a pressure detection unit, a temperature and humidity detection unit, and an execution status acquisition unit; The device also includes a collaborative closed-loop control module, which is connected to the gas concentration detection unit, pressure detection unit, temperature and humidity detection unit, execution status acquisition unit, reaction supply component, fan, and air inlet valve of the first and second marking chambers, respectively. It is used to synchronously acquire gas concentration information, pressure difference information, temperature and humidity information, concentration change trend information, and execution component status information of the first and second marking chambers. Based on the concentration difference, pressure difference relationship, concentration decay characteristics, temperature and humidity changes, and execution component status of the two chambers, it performs collaborative judgment on the dual-chamber operating conditions to distinguish between the normal absorption state of the plant, the supply deviation state, the sealing leakage state, and the inter-chamber cross-ventilation state. Based on the judgment results, it performs linkage compensation control on the input rate of the reaction supply components on both sides, the opening degree of the air inlet valve, and the operating parameters of the fan.

[0006] Furthermore, the sealing assembly includes a nested annular groove structure disposed at the bottom of the transparent marking chamber, wherein the nested annular groove structure is filled with liquid to form a water seal structure.

[0007] Furthermore, the transparent marking chamber includes a large glass cover and a small glass cover nested at the bottom of the large glass cover, the small glass cover and the soil box forming the water seal structure.

[0008] Furthermore, the collaborative closed-loop control module is configured to execute the following decision logic: When the concentration in one labeling chamber decreases and no corresponding abnormal increase occurs in another labeling chamber, the pressure difference is within the preset stable range, and the execution status is normal, it is determined to be a normal absorption state of the plant. When the concentration in a certain labeling chamber decreases and the corresponding execution status shows that the valve is not opened as set, the acid injection is abnormal, or the fan is running abnormally, it is determined to be a supply deviation state. When the concentration in a certain marking chamber decreases and the pressure difference between that marking chamber and the outside environment changes abnormally, while the other marking chamber does not show a corresponding abnormal increase, it is determined to be a state of sealing leakage. When the concentration in one labeled chamber decreases and the corresponding abnormal increase occurs in another labeled chamber, or when the pressure difference between the two chambers is abnormally reversed, it is determined to be an inter-chamber cross-flow.

[0009] Furthermore, the reaction supply component includes a chemical reaction generating unit, which can switch between a chemical reaction release mode and an external direct input mode; when performing carbon isotope labeling, labeled carbon dioxide is generated by injecting acid into a container containing carbonates; when performing nitrogen isotope labeling, labeled nitrogen gas is introduced into the labeling chamber where legumes are grown through an external direct input mode.

[0010] Furthermore, the chemical reaction generating unit includes a beaker fixing slide groove disposed on the inner wall of the transparent marking chamber and a first beaker mounted on the beaker fixing slide groove, the beaker fixing slide groove being used to adjust the height of the first beaker.

[0011] In a second aspect of the invention, a method for carbon and nitrogen isotope dual-chamber labeling and control in an intercropping system is provided, the method comprising: Step S1: Plant different plants in the root growth areas on both sides of the removable partition, and install the first and second marking chambers above the soil box to form a sealed space through the sealing component; Step S2: Provide the corresponding marking medium to the first marking chamber and the second marking chamber respectively, and start the fan to promote indoor air circulation; Step S3: The collaborative closed-loop control module synchronously collects gas concentration information, pressure difference information, temperature and humidity information, concentration change trend information, and actuator status information from the first and second labeling chambers. Step S4: The collaborative closed-loop control module performs collaborative judgment on the dual-chamber operating conditions based on the concentration difference, pressure difference relationship, concentration decay characteristics, temperature and humidity changes and the status of the actuators in the two chambers, so as to distinguish the normal absorption state of the plant, the supply deviation state, the sealing leakage state and the inter-chamber air leakage state. Step S5: Based on the determination result of step S4, perform linkage compensation control on the input rate of the reaction supply components on both sides, the opening degree of the intake valve and the fan operating parameters to maintain the target marking environment of each chamber and suppress gas crosstalk.

[0012] Furthermore, it also includes step S6: after marking is completed, the removable partition is removed, and plant and soil samples are collected for isotope abundance determination.

[0013] The beneficial technical effects of the present invention are at least as follows: To address the aforementioned problems, this invention provides a carbon and nitrogen isotope dual-chamber labeling and control device and method for intercropping systems. Through the synergy of a soil box partition structure, a removable partition, and a dual-chamber water seal structure, it achieves zoned isolation labeling of the aboveground parts of different plants within the intercropping system, as well as continuous integration for subsequent root interaction studies of the underground parts. Furthermore, a collaborative closed-loop control module jointly analyzes and determines the operating parameters and conditions of the first and second labeling chambers. This allows the control logic to move beyond single-chamber concentration threshold replenishment, distinguishing between normal plant absorption and supply deviations, water seal leakage, and inter-chamber cross-ventilation. Based on this, it implements coordinated compensation control of the reaction supply components, air inlet valves, and fans on both sides, thereby improving the stability of the dual-chamber labeling process, reducing the risk of cross-contamination, and enhancing the accuracy and repeatability of isotope labeling. In addition, the reaction supply components of this invention support switching between chemical reaction release mode and external direct input mode, and can adjust the supply position according to the plant growth status, thus exhibiting good experimental adaptability and application flexibility. Attached Figure Description

[0014] The present invention will be further described with reference to the accompanying drawings, but the embodiments in the drawings do not constitute any limitation on the present invention. For those skilled in the art, other drawings can be obtained based on the following drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of a device for simultaneously labeling different plants with carbon and nitrogen isotopes in an intercropping system, as described in this invention.

[0016] Figure 2 For the present invention Figure 1 Enlarged structural schematic diagram of key components of the reaction supply assembly; where A is an enlarged view of a beaker with a slider structure, and B is a cross-sectional view of the air inlet valve and its internal ventilation structure.

[0017] Figure 3 The rhizosphere soil in Example 1 15 Figure showing the results of N isotope determination.

[0018] Figure 4 The plant in Example 1 15 Figure showing the results of N isotope determination.

[0019] The attached diagrams are labeled as follows: 1. Fan; 2. Large glass cover; 3. Beaker; 4. Small glass cover; 5. Connecting clip; 6. Beaker fixing groove; 7. Removable partition; 8. Soil box. Detailed Implementation

[0020] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0021] In one or more embodiments, such as Figure 1 As shown, a method for carbon and nitrogen isotope dual-chamber labeling and control in intercropping systems is disclosed, the method comprising the following: The device includes: The soil box 8 at the bottom and the marking assembly above the soil box 8, the interior of the soil box 8 is divided into two independent root growth areas by a removable partition 7; The marking assembly includes a first marking chamber and a second marking chamber respectively corresponding to two root growth areas. The first marking chamber and the second marking chamber are respectively formed by a transparent marking chamber body located above the soil box 8. A sealing assembly is provided at the bottom of the transparent marking chamber body where it connects to the soil box 8 to form an airtight connection. The first and second labeling chambers are respectively equipped with a reaction supply component, a fan 1, a gas concentration detection unit, a pressure detection unit, a temperature and humidity detection unit, and an execution status acquisition unit; The device also includes a collaborative closed-loop control module, which is connected to the gas concentration detection unit, pressure detection unit, temperature and humidity detection unit, execution status acquisition unit, reaction supply component, fan 1, and air inlet valve of the first and second marking chambers, respectively. It is used to synchronously collect gas concentration information, pressure difference information, temperature and humidity information, concentration change trend information, and execution component status information of the first and second marking chambers. Based on the concentration difference, pressure difference relationship, concentration decay characteristics, temperature and humidity changes, and execution component status of the two chambers, it performs collaborative judgment on the dual-chamber operating conditions to distinguish between the normal absorption state of the plant, the supply deviation state, the sealing leakage state, and the inter-chamber cross-ventilation state. Based on the judgment results, it performs linkage compensation control on the input rate of the reaction supply components on both sides, the opening degree of the air inlet valve, and the operating parameters of fan 1.

[0022] Example 1: This embodiment details the specific process of pulse marking using the device of the present invention. The experiment was conducted using maize, a gramineous crop. Zea mays L. ) and soybean (a legume) Glycine max (L.) Merr. ( ) were used as model plants. First, soil boxes 8 were assembled using connecting clips 5, and a removable partition 7 was inserted in the middle. Corn and soybeans were planted in the root growth areas on both sides of the partition, respectively. They were cultured in a controlled laboratory environment for 30 days, with the culture conditions set as a 12 h / 12 ​​h photoperiod and a light intensity of 600 μmol·m⁻¹.-2 ·s -1 The daytime and nighttime temperatures were 25±2°C and 20±2°C, respectively.

[0023] Once the plants have grown to the marking stage, pulse marking will be conducted for two days, with five pulses per day. Before the formal marking, the large glass dome 2 is placed above the soil box 8, and a water seal is formed by filling the small glass dome 4 at the bottom with liquid. Carbon marking is performed on the corn in the first marking chamber: the beaker 3 on the beaker fixing groove 6 is adjusted to the height of the densely packed corn leaves, and the reaction reagent is injected into the beaker 3 using an injection needle, so that it reacts with the... The reaction produces Nitrogen labeling is performed on soybeans in the second labeling chamber: air is introduced into the second labeling chamber through the intake valve and rubber hose. Gases were used to label leguminous plants with nitrogen isotopes. During the labeling process, both the first and second labeling chambers were in actual labeling operation. The coordinated closed-loop control module synchronously received environmental data and actuator feedback from both sides, and organized the data from the device itself into the timing objects required for subsequent judgment. The first and second labeling chambers... The concentrations are respectively determined by non-dispersive infrared radiation installed on the upper sidewalls of their respective shrouds. Sensor data acquisition: The pressure difference between the two marking chambers and the outside environment is acquired by micro differential pressure sensors; the temperature and humidity of the two marking chambers are obtained by digital temperature and humidity integrated sensors; the acid injection rate of the first marking chamber is obtained by the set flow rate and feedback flow rate returned by the injection pump drive board; the air intake status of the second marking chamber is returned by a proportional valve or mass flow controller, preferably using valve opening as the control variable; the operating status of the fans 1 in both marking chambers is obtained by Hall effect speed measurement signals. The collaborative closed-loop control module preferably... Simultaneous sampling is performed once, and data is continuously collected at 12 time points to ensure that a single analysis window covers [the data]. The continuous operation process. The controller organizes environmental quantities into a dual-chamber observation sequence. Its preferred format is The matrix has 8 channels, which are sequentially designated as the first marking chamber. Concentration, Second Labeling Chamber Concentration, pressure difference in the first labeling chamber, pressure difference in the second labeling chamber, temperature in the first labeling chamber, temperature in the second labeling chamber, humidity in the first labeling chamber, and humidity in the second labeling chamber; the controller organizes the actuator data into an execution state sequence. Its preferred format is The matrix has six channels, representing the acid injection rate of the first labeling chamber, the valve opening of the second labeling chamber, the fan speed of the first labeling chamber, the fan speed of the second labeling chamber, the acid injection feedback value of the first labeling chamber, and the valve feedback value of the second labeling chamber, respectively. If the first labeling chamber... Concentration from Slowly descending to Second marking room Concentration maintained at The pressure difference in the vicinity and the first marking chamber stabilized at Nearby, and the error between the set value and the feedback value of the first marking chamber injection pump is less than Then the window is in and The system forms a combined mode of "single-chamber slow descent, relative chamber stability, pressure difference stability, and consistent execution"; if the concentration of the first labeled chamber decreases while the concentration of the second labeled chamber increases simultaneously, and the pressure difference relationship between the two chambers reverses within the window, then the window forms a combined mode of "abnormal cross-chamber concentration coupling and abnormal pressure difference relationship".

[0024] Collaborative closed-loop control module receives and Subsequently, the current operating conditions of the dual-marker chamber are identified, and the control increments required for linkage compensation are generated. A dual-branch timing fusion network is preferably deployed internally within the controller, where the first branch processes... It consists of two layers of one-dimensional causal convolutions. The first layer has 16 convolutional kernels with a kernel length of 3, and the second layer has 32 convolutional kernels with a kernel length of 3. Each layer is followed by a ReLU activation layer and a batch normalization layer to extract the environmental evolution pattern of the dual-label chamber within an analysis window; the second branch processes... First, the data passes through a one-dimensional causal convolutional layer with 16 kernels and a length of 3, followed by a fully connected layer with 16 nodes to extract temporal matching features for acid injection, valve action, and fan speed. After aligning the two features in the temporal dimension, they are input into a single-layer GRU with 24 hidden units. The hidden state of the GRU at the last time step is denoted as... The collaborative closed-loop control module simultaneously generates four maintenance positive vectors based on the physical operation characteristics within the current window. The probability vector for the current operating condition is calculated as follows: The first component represents the percentage of sampling points where "single-chamber concentration decreases, opposite-chamber concentration does not increase, differential pressure is stable, and execution is consistent"; the second component represents the percentage of sampling points where "the deviation between the set flow rate and the feedback flow rate exceeds the set threshold, valve position feedback is lagging, and fan speed is lower than the target value"; the third component represents the percentage of sampling points where "the differential pressure between a single chamber and the outside continuously deviates from the stable range and the concentration in the opposite chamber does not increase synchronously"; and the fourth component represents the percentage of sampling points where "the concentration in one chamber decreases while the concentration in another chamber increases, or the differential pressure relationship between the two chambers reverses." Calculate using the following formula: ; in, This is a four-dimensional probability vector of operating conditions, with the four components representing the probabilities of normal plant absorption, supply deviation, water seal leakage, and inter-chamber air leakage, respectively. The output layer weight matrix is ​​derived from fixed parameters obtained during offline training using labeled samples in the device debugging phase; For dual-branch convolutional networks and single-layer GRU pairs and The final hidden state after processing; This is a physical rule correction coefficient, with a preferred value range of [value range missing]. to ; This is a four-dimensional physical rule correction vector, where each component is... arrive The ratio between them. The collaborative closed-loop control module then generates a control increment vector based on the control template. Plants normally absorb templates The preferred setting is to increase the acid injection rate in the first marking chamber. The valve opening in the second marking chamber is increased. The fan speed in the first marking chamber increased. The fan speed in the second marking chamber increased. Supply Deviation Template The preferred setting is to increase the acid injection rate in the first marking chamber. The valve opening in the second marking chamber is increased. The speeds of both fans increased. Water seal leakage template The preferred setting is to reduce the acid injection rate in the first labeling chamber. The valve opening in the second marking chamber is reduced. The speeds of both fans were reduced. Inter-room ventilation template The preferred setting is to reduce the acid injection rate in the first labeling chamber. The valve opening in the second marking chamber is reduced. The speeds of both fans were reduced. The control increment is calculated using the following formula: ; in, The four-dimensional control increment vector consists of four components that correspond to the adjustment amount of the acid injection rate in the first marking chamber, the adjustment amount of the valve opening in the second marking chamber, the adjustment amount of the fan speed in the first marking chamber, and the adjustment amount of the fan speed in the second marking chamber, respectively. The working condition probability vector The One component; For the first The control template vector corresponding to the type of working condition, where Control template corresponding to the normal absorption state of plants, Control template corresponding to supply deviation status, Control template corresponding to water seal leakage status, Control template corresponding to inter-room cross-ventilation. Under a set of representative configuration parameters, if The adjustment amount of the acid injection rate in the first marking chamber is: The valve opening adjustment amount for the second marking chamber is: The speed adjustment of both fans is... If the original setting of the first marking chamber injection pump is at this time... The original opening degree of the valve in the second marking chamber was... The original speed of the two fans was The new execution settings for this round are as follows: , and The collaborative closed-loop control module sends the above control increments to the injection pump driver of the first marking chamber, the proportional valve driver module of the second marking chamber, and the PWM fan driver modules of the two marking chambers, respectively, and records the control commands and execution feedback for this round. After the marking is completed, the partition (8) is removed to simulate the root interaction stage, and finally plant samples and rhizosphere soil of the zone are collected for isotope abundance determination.

[0025] Experimental results are as follows Figure 3 As shown, the measurement data indicated that after two days of pulse labeling, the 15N value in the soybean rhizosphere soil of the second labeling chamber significantly increased from the background level of 2.75‰ to 4.75‰; simultaneously, a significant enrichment characteristic was also detected in the maize rhizosphere soil of the first labeling chamber, with its 15N value increasing from 2.29‰ to 3.15‰. These results demonstrate that under the experimental conditions of dual-labeling chambers with zoned labeling and subsequent restoration of root interaction, labeled nitrogen in the second labeling chamber can migrate to the corresponding root zone in the first labeling chamber, providing a basis for studying the underground nutrient transfer process in intercropping systems.

[0026] Experimental results are as follows Figure 4 As shown: Isotope abundance measurements at the plant level further validated the regional labeling efficiency of this device in complex intercropping systems. The soybean plants in the second labeling chamber had a plant-wide average 15N content of 22.07‰, demonstrating high labeling assimilation efficiency; the 15N content in the maize plants in the first labeling chamber increased from 2.29‰ to 5.05‰. Combined with... Figure 3 The rhizosphere soil results shown, as well as the inhibitory effect of water seal structure and synergistic closed-loop control on gas phase crosstalk, indicate that this device can provide relatively reliable experimental conditions for analyzing underground nitrogen transfer and species interaction processes in intercropping systems.

[0027] Example 2: Application of dual-marker chamber collaborative closed-loop control. In this example, the collaborative closed-loop control module continuously acquires data from the first and second marker chambers according to a preset sampling period. Concentration, pressure difference, temperature and humidity, concentration change trends, valve opening and closing status, acid injection status, and fan operation status were recorded, and these environmental parameters were compiled into a dual-chamber observation sequence. Organize the actuator states into an execution state sequence. Subsequently, the collaborative closed-loop control module utilizes a dual-branch temporal fusion network and physical rule correction vectors. Calculate the probability vector of the current operating condition. And based on the templates for four types of working conditions , , and Generate control increment vector Then The control is distributed to the injection pump, proportional valve, and fan drive module to achieve coordinated compensation control of the dual labeling chambers. Under normal absorption conditions, one labeling chamber... When the concentration decreases, no corresponding abnormal increase is observed in the other labeling chamber, the differential pressure remains stable, and the actuator feedback is normal, the collaborative closed-loop control module generates a control increment primarily focused on light replenishment. In a supply deviation scenario, if valve feedback deviates from the set value, acid injection flow feedback is lower than the target value, or fan speed is lower than the set value, the collaborative closed-loop control module generates a control increment primarily focused on correcting the supply. In a water seal leakage scenario, if the differential pressure between one labeling chamber and the outside continuously deviates from the stable range and the concentration in the other chamber does not increase synchronously, the collaborative closed-loop control module generates a control increment primarily focused on reducing the supply intensity and minimizing fan disturbance. In an inter-chamber cross-venting scenario, if the concentration in one labeling chamber decreases while the concentration in another labeling chamber increases, or the differential pressure relationship between the two chambers reverses, the collaborative closed-loop control module generates a control increment primarily focused on simultaneously reducing the supply and decreasing the intensity of both-sided circulation. Taking a representative calculation result as an example, if the current window operating condition probability vector... Based on the control template, the adjustment amount of the acid injection rate in the first marking chamber can be calculated as follows: The valve opening adjustment amount for the second marking chamber is: The speed adjustment of both fans is... When the original setting value of the first marking chamber injection pump is... The original opening degree of the valve in the second marking chamber was... The original speed of the two fans was At that time, the updated execution settings are as follows: , and After execution, the controller outputs the actual control status. and abnormal signs ,in This includes the setpoints and feedback values ​​for the syringe pump, proportional valve, and fan drive module. This is used to record whether the system enters the water seal leakage suppression mode or the inter-chamber crosstalk suppression mode. Through this dual-marker chamber cooperative closed-loop control method, the first and second marker chambers can maintain their respective target marking environments in the same interleaving system, and effectively reduce the risk of gas crosstalk during the parallel operation of the two chambers.

[0028] The working principle of this invention is based on an intelligent closed-loop control logic of "perception-decision-execution," achieving precise regulation of the isotope labeling environment of the intercropping system through a dual-labeling chamber collaborative closed-loop control module. The system first uses a sensor array to collect real-time data on gas concentration, temperature, humidity, pressure difference, and actuator status within the dual-labeling chamber, forming a dual-chamber observation sequence. The controller calculates the concentration difference between the two chambers and the relative external pressure difference, combining historical data with preset operating condition feature vectors to accurately identify the current state, such as "normal plant absorption," "supply deviation," "seal leakage," or "inter-chamber cross-ventilation." Subsequently, the system calls a dual-branch temporal fusion network and a physical rule correction model to calculate the optimal control increment and generate a linkage compensation command. Finally, the actuator synchronously adjusts the acid injection rate, valve opening, and fan speed, dynamically maintaining the stability of the concentration and pressure difference within the dual-labeling chamber through a negative feedback mechanism, achieving continuous integration of zoned isolation labeling of different plant aboveground parts and interactive research on underground root systems.

[0029] This invention achieves significant beneficial effects through the aforementioned technical solution. First, by utilizing the removable partition 7 and a dual-chamber independent monitoring mechanism, combined with a collaborative closed-loop control algorithm, crosstalk of the labeled gas between the two chambers can be effectively suppressed, keeping the cross-contamination rate at an extremely low level and ensuring the purity and accuracy of isotope labeling. Second, by introducing a neural network model with physical rule corrections, not only is the system's ability to predict plant absorption trends improved, but physical inconsistencies that may arise from purely data-driven models are also avoided, making the control process more stable and reliable, and significantly reducing the concentration fluctuation range in the labeling chambers. Finally, the system possesses full-condition adaptive capability, automatically identifying abnormal states and activating corresponding compensation or alarm mechanisms, greatly improving the intelligence, ease of operation, and data reliability of carbon and nitrogen isotope labeling experiments in intercropping systems.

[0030] It is worth noting that the specific working process of the carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems provided in this embodiment of the invention is the same as that of the carbon and nitrogen isotope dual-chamber labeling and control method for intercropping systems described in the above embodiment, and will not be repeated here.

[0031] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems, comprising a soil box at the bottom and a labeling assembly disposed above the soil box, characterized in that: The soil box is divided into two independent root growth areas by a removable partition (7); The marking assembly includes a first marking chamber and a second marking chamber respectively corresponding to two root growth zones. The first marking chamber and the second marking chamber are respectively formed by a transparent marking chamber body located above the soil box. A sealing assembly is provided at the bottom of the transparent marking chamber body where it connects to the soil box to form an airtight connection. The first and second labeling chambers are respectively equipped with a reaction supply component, a fan (1), a gas concentration detection unit, a pressure detection unit, a temperature and humidity detection unit, and an execution status acquisition unit; The device also includes a collaborative closed-loop control module, which is connected to the gas concentration detection unit, pressure detection unit, temperature and humidity detection unit, execution status acquisition unit, reaction supply component, fan (1) and air inlet valve of the first and second marking chambers respectively. It is used to synchronously collect gas concentration information, pressure difference information, temperature and humidity information, concentration change trend information and execution component status information of the first and second marking chambers, and to collaboratively determine the working conditions of the two chambers based on the concentration difference, pressure difference relationship, concentration decay characteristics, temperature and humidity changes and execution component status, so as to distinguish the normal absorption state of the plant, the supply deviation state, the sealing leakage state and the inter-chamber cross-ventilation state, and to perform linkage compensation control on the input rate of the reaction supply component on both sides, the opening degree of the air inlet valve and the operating parameters of the fan (1) according to the determination results.

2. The carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems according to claim 1, characterized in that, The sealing assembly includes a nested annular groove structure located at the bottom of the transparent marking chamber, and the nested annular groove structure is filled with liquid to form a water seal structure.

3. The carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems according to claim 2, characterized in that, The transparent marking chamber includes a large glass cover (2) and a small glass cover (4) nested at the bottom of the large glass cover (2). The small glass cover (4) cooperates with the soil box to form the water seal structure.

4. The carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems according to claim 1, characterized in that, The collaborative closed-loop control module is configured to execute the following decision logic: When the concentration in one labeling chamber decreases and no corresponding abnormal increase occurs in another labeling chamber, the pressure difference is within the preset stable range, and the execution status is normal, it is determined to be a normal absorption state of the plant. When the concentration in a certain labeling chamber decreases and the corresponding execution state shows that the valve is not opened as set, the acid injection is abnormal, or the fan (1) is running abnormally, it is determined to be a supply deviation state; When the concentration in a certain marking chamber decreases and the pressure difference between that marking chamber and the outside environment changes abnormally, while the other marking chamber does not show a corresponding abnormal increase, it is determined to be a state of sealing leakage. When the concentration in one labeled chamber decreases and the corresponding abnormal increase occurs in another labeled chamber, or when the pressure difference between the two chambers is abnormally reversed, it is determined to be an inter-chamber cross-flow.

5. The carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems according to claim 1, characterized in that, The reaction supply component includes a chemical reaction generating unit that can switch between a chemical reaction release mode and an external direct input mode. When performing carbon isotope labeling, labeled carbon dioxide is generated by injecting acid into a container containing carbonates. When performing nitrogen isotope labeling, labeled nitrogen gas is introduced into the labeling chamber where legumes are grown through an external direct input mode.

6. The carbon and nitrogen isotope dual-chamber labeling and control device for intercropping systems according to claim 5, characterized in that, The chemical reaction generating unit includes a beaker (3) fixing groove disposed on the inner wall of the transparent marking chamber and a first beaker (3) mounted on the beaker (3) fixing groove. The beaker (3) fixing groove is used to adjust the height of the first beaker (3).

7. A method for isotope labeling of an intercropping system using the apparatus described in any one of claims 1 to 6, characterized in that, The method includes the following steps: Step S1: Plant different plants in the root growth areas on both sides of the removable partition (7), and install the first and second marking chambers above the soil box to form a sealed space through the sealing component; Step S2: Provide the corresponding marking medium to the first marking chamber and the second marking chamber respectively, and start the fan (1) to promote indoor gas circulation; Step S3: The collaborative closed-loop control module synchronously collects gas concentration information, pressure difference information, temperature and humidity information, concentration change trend information, and actuator status information from the first and second labeling chambers. Step S4: The collaborative closed-loop control module performs collaborative judgment on the dual-chamber operating conditions based on the concentration difference, pressure difference relationship, concentration decay characteristics, temperature and humidity changes and the status of the actuators in the two chambers, so as to distinguish the normal absorption state of the plant, the supply deviation state, the sealing leakage state and the inter-chamber air leakage state. Step S5: Based on the determination result of step S4, the input rate of the reaction supply components on both sides, the opening degree of the intake valve and the operating parameters of the fan (1) are controlled in a coordinated manner to maintain the target marking environment of each chamber and suppress gas crosstalk.

8. The method for isotope labeling of an intercalation system using the apparatus described in any one of claims 1 to 6 according to claim 7, characterized in that, The method also includes step S6: after marking is completed, remove the removable partition (7) and collect plant and soil samples for isotope abundance determination.

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