A soothing repair composition of plant origin and its use

Abstract

The present application relates to a soothing and repairing composition of plant origin and its application, and belongs to the technical field of cosmetics. The soothing and repairing composition comprises Dicksonia antarctica extract, Dendrobium nobile Lindl extract, Panacis majoris Rhizoma extract and Fagopyrum esculentum Moench seed extract, the components synergize with each other, inhibit inflammatory response, scavenge oxidative free radicals, reduce skin nerve stimulation sensitivity, regulate skin immune state, simultaneously strengthen skin barrier and improve skin microcirculation, comprehensively soothe skin redness, stinging, itching and other discomforts, realize calming and repairing and stability maintaining effects, and can be widely applied to preparation of cosmetics in different dosage forms. Effect verification shows that the soothing and repairing composition has significant hyaluronidase inhibition capacity and neutrophil inhibition capacity, the nanoemulsion prepared from the soothing and repairing composition has the advantages of timely soothing of skin irritation and long-acting soothing and repairing effect, high permeability, high stability, refreshing skin feeling and safety and non-irritation.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to a plant-derived soothing and repairing composition and its application. Background Technology

[0002] In recent years, influenced by multiple factors such as environmental pollution, ultraviolet radiation, irregular sleep patterns, and frequent use of highly active functional skincare products, skin is prone to problems such as sensitivity, redness, dryness, tightness, stinging, and discomfort. Skin barrier damage is becoming increasingly common, leading to a sustained increase in market demand for skincare ingredients and products that combine gentle soothing and barrier repair functions. Compared to traditional chemical soothing ingredients, plant-derived soothing ingredients have become a research and application hotspot in the skincare field due to their superior safety, low irritation, and good skin compatibility, possessing promising prospects for industrialization and market application. However, current plant-based soothing formulas suffer from technical problems such as limited efficacy, poor stability, or an inability to simultaneously provide immediate soothing and long-lasting repair. Therefore, there is an urgent need for a scientifically formulated, highly compatible, stable, and gentle plant-derived soothing and repairing composition. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a plant-derived soothing and repairing composition and its application.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a soothing and repairing composition comprising the following components in parts by weight: 2-10 parts of *Dictamnus longifolia* extract, 2.5-7 parts of *Dendrobium nobile* extract, 0.5-8 parts of *Codonopsis pilosula* extract, and 0.5-8 parts of buckwheat seed extract.

[0005] This invention utilizes extracts of *Dictamnus sylvestris* (L.), *Dendrobium nobile* (L.), *Anthriscus sylvestris* (L.), and buckwheat seed extract to create a soothing and repairing composition. *Dictamnus sylvestris* extract is rich in polysaccharides, amino acids, and antioxidants. Its polysaccharides help the skin retain moisture, enhance barrier function, and reduce dryness and roughness. *Dictamnus sylvestris* extract also possesses antioxidant properties; its polysaccharides and polyphenols synergistically neutralize free radicals, thereby exerting moisturizing, antioxidant, and soothing effects. *Dendrobium nobile* extract is rich in polysaccharides, polyphenols, and flavonoids, which can enhance the skin barrier, neutralize free radicals, reduce inflammation and soothe, and reduce redness and irritation, making it suitable for moisturizing and soothing sensitive skin. *Anthriscus sylvestris* (L.)... Hoffm. is a biennial or perennial herb belonging to the Apiaceae family and the Hoffm. It contains various chemical components such as phenylpropanoids, flavonoids, steroids, fatty acids, and organic acids. These components endow Hoffm. with various pharmacological effects, including antioxidant, antibacterial, anti-inflammatory, and immunomodulatory properties. Buckwheat seed extract is rich in macromolecular polysaccharides, which can form a film on the skin surface. This film can enhance the skin barrier function and lock in moisture, resulting in a significant lifting, firming, and softening effect. The above components work synergistically to inhibit inflammatory responses, scavenge free radicals, reduce skin nerve stimulation sensitivity, regulate skin immunity, strengthen the skin barrier, improve skin microcirculation, and comprehensively soothe skin redness, stinging, dryness, itching, and other discomforts, achieving a calming, repairing, and stabilizing effect.

[0006] Furthermore, the soothing and repairing composition comprises the following components in parts by weight: 4-8 parts of Bambusa textilis extract, 4.5-5.5 parts of Dendrobium nobile extract, 1-6 parts of Panax quinquefolius extract, and 1-6 parts of buckwheat seed extract.

[0007] Furthermore, the preparation method of the extracts of *Dictyophora indicum*, *Dendrobium nobile*, *Gynostemma pentaphyllum*, and buckwheat seed extract includes the following steps: The fruiting bodies of *Dictyophora indicum*, stems of *Dendrobium nobile*, roots of *Gynostemma pentaphyllum*, and seeds of buckwheat were pulverized to obtain *Dictyophora indicum* powder, *Dendrobium nobile* powder, *Gynostemma pentaphyllum* powder, and buckwheat seed powder, respectively. The *Dictyophora indicum* powder, *Dendrobium nobile* powder, *Gynostemma pentaphyllum* powder, and buckwheat seed powder were each mixed with deionized water at a ratio of 1g:30-40mL. The mixture was refluxed at 90-100℃ for 2 hours. The residue was filtered off, and the filtrate was concentrated to a relative density of 1.04-1.08. Ethanol was added to a concentration of 60% v / v-70% v / v, and the mixture was allowed to stand at room temperature for 6 hours for alcohol precipitation. The residue was filtered off, and the supernatant was collected. The ethanol in the supernatant was recovered until no alcohol odor remained, and then freeze-dried to constant weight to obtain *Dictyophora indicum* extract, *Dendrobium nobile* extract, *Gynostemma pentaphyllum* extract, and buckwheat seed extract, respectively.

[0008] Secondly, the present invention provides the use of the soothing and repairing composition described in the first aspect in the preparation of cosmetics.

[0009] Furthermore, the amount of the soothing and repairing composition added to the cosmetic is 0.5wt%-5wt%.

[0010] Furthermore, the dosage form of the cosmetic is selected from aqueous solutions, emulsions, creams, gels, or masks.

[0011] Thirdly, the present invention provides an aqueous cosmetic containing the following components in weight percentage: 0.5%-10% of the soothing and repairing composition described in the first aspect, 45%-55% of glycerin, 0.5%-0.6% of 1,2-hexanediol, 0.1%-0.5% of caprylyl glycol, and the balance being deionized water.

[0012] Fourthly, the present invention provides a nanoemulsion cosmetic, prepared from the following components in weight percentage: 0.5%-5% of the soothing and repairing composition described in the first aspect, 5%-10% of medium-chain triglycerides, 2%-3% of polyglycerol-6-laurate, 0.9%-1.2% of polyglycerol-4-oleate, 3%-6% of glycerol, 0.5%-5% of 1,2-hexanediol, 0.1%-0.5% of caprylyl glycol, and the balance being deionized water.

[0013] In this invention, polyglycerol-6-laurate acts as the primary emulsifier to form an O / W interfacial film, while polyglycerol-4-oleate assists in stabilizing the interfacial film. Together, they form a stable O / W emulsion system, which, combined with mechanical shearing, forms nano-sized droplets, giving it a refreshing feel. These nano-sized droplets exhibit excellent stability and transdermal permeability, penetrating deep into hair follicles and the stratum corneum, significantly improving the transdermal absorption rate of active ingredients. Combined with the emulsifying and moisturizing effects of glycerin and caprylyl glycol, the system possesses both good physical stability and gentleness, effectively protecting the active ingredients. Simultaneously, the combination of 1,2-hexanediol and caprylyl glycol in the system provides antibacterial effects, eliminating the need for traditional preservatives and contributing to improved product safety. Therefore, the nanoemulsion provided by this invention has the advantages of high penetration, high stability, a refreshing feel, and is safe and non-irritating.

[0014] Furthermore, the preparation method of the nanoemulsion cosmetic includes the following steps: S1. Medium-chain triglycerides, polyglycerol-6-laurate, polyglycerol-4-oleate, 1,2-hexanediol and octyl glycol are mixed evenly to obtain phase A; S2. Mix glycerin and two-thirds of the amount of deionized water thoroughly to obtain phase B; S3. Mix the soothing and repairing composition with the remaining deionized water until homogeneous to obtain phase C; S4. Slowly add phase B to phase A, stir and mix, then add phase C, homogenize under high pressure to obtain nanoemulsion.

[0015] Further, the mixing in step S1 is carried out by stirring at 60-70°C and 200-400 r / min.

[0016] Further, the mixing in step S2 is carried out by stirring at 60-70°C and 200-400 r / min.

[0017] Further, the mixing described in step S3 is performed by stirring at 35-45°C and 200-400 r / min.

[0018] Further, the mixing conditions in step S4 are: rotation speed 300-600 r / min, time 20-30 min.

[0019] Furthermore, the conditions for high-pressure homogenization in step S4 are: pressure 800-1000 bar / min, homogenization speed 4000-10000 r / min, temperature 35-45℃, and number of cycles 3-5.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a plant-derived soothing and repairing composition. The components of this composition synergistically inhibit inflammatory responses, scavenge free radicals, reduce skin's sensitivity to nerve stimulation, regulate skin's immune status, strengthen the skin barrier, and improve skin microcirculation. It comprehensively soothes skin discomfort such as redness, stinging, dryness, and itching, achieving calming, repairing, and stabilizing effects. This composition can be widely used in the preparation of cosmetics in various formulations. Efficacy verification shows that the soothing and repairing composition has significant hyaluronidase inhibition and neutrophil inhibition capabilities. Nanoemulsions prepared using the soothing and repairing composition provided by this invention provide immediate relief from skin irritation and achieve long-lasting soothing and repairing effects. They also possess the advantages of high penetration, high stability, a refreshing feel, and safety without irritation. Detailed Implementation

[0021] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0022] The materials used in the following embodiments are prepared by the following methods: Extract of Dictyophora indusiate: Prepared using the fruiting body of Dictyophora indusiate (Vent. ex Pers) Fisch (commercially available); the specific preparation method includes the following steps: The fruiting bodies of *Dictyophora indusiata* were pulverized to obtain *Dictyophora indusiata* powder. The *Dictyophora indusiata* powder and deionized water were mixed evenly at a material-to-liquid ratio of 1g:30mL. The mixture was refluxed at 90℃ for 2 hours. The residue was filtered off, and the filtrate was concentrated to a relative density of 1.04-1.08. Ethanol was added to a concentration of 60% v / v. The mixture was allowed to stand at room temperature for 6 hours for alcohol precipitation. The residue was filtered off, and the supernatant was collected. The ethanol in the supernatant was recovered until no alcohol odor was detected, and then the mixture was freeze-dried to constant weight to obtain *Dictyophora indusiata* extract.

[0023] Dendrobium nobile extract: using Dendrobium nobile ( Dendrobium nobile It was prepared from the stem of Lindl. (commercially available); the specific preparation method includes the following steps: The stems of Dendrobium nobile were pulverized to obtain Dendrobium nobile powder. The Dendrobium nobile powder and deionized water were mixed evenly at a material-to-liquid ratio of 1g:40mL. The mixture was refluxed at 95℃ for 2h, filtered to remove the residue, and the filtrate was concentrated to a relative density of 1.04-1.08. Ethanol was added to a concentration of 70% v / v, and the mixture was allowed to stand at room temperature for 6h for alcohol precipitation. The residue was filtered to remove the residue, and the supernatant was collected. The ethanol in the supernatant was recovered until no alcohol odor was detected, and then the mixture was freeze-dried to constant weight to obtain Dendrobium nobile extract.

[0024] Emei ginseng extract: using Emei ginseng ( Anthriscus sylvestris It was prepared from the root of (L.) Hoffm. (commercially available); the specific preparation method includes the following steps: The roots of *Codonopsis pilosula* were crushed to obtain *Codonopsis pilosula* powder. The *Codonopsis pilosula* powder and deionized water were mixed evenly at a material-to-liquid ratio of 1g:40mL. The mixture was refluxed at 95℃ for 2 hours. The residue was filtered off and the filtrate was concentrated to a relative density of 1.04-1.08. Ethanol was added to a concentration of 70% v / v. The mixture was allowed to stand at room temperature for 6 hours for alcohol precipitation. The residue was filtered off and the supernatant was collected. The ethanol in the supernatant was recovered until there was no alcohol odor. The supernatant was then freeze-dried to constant weight to obtain the *Codonopsis pilosula* extract.

[0025] Buckwheat seed extract: Use buckwheat ( Fagopyrum esculentum It is prepared from seeds of Moench (commercially available); the specific preparation method includes the following steps: Buckwheat seeds were crushed to obtain buckwheat seed powder. The buckwheat seed powder and deionized water were mixed evenly at a material-to-liquid ratio of 1g:30mL. The mixture was refluxed at 90℃ for 2h. The residue was filtered off and the filtrate was concentrated to a relative density of 1.04-1.08. Ethanol was added to a concentration of 60% v / v. The mixture was allowed to stand at room temperature for 6h for alcohol precipitation. The residue was filtered off and the supernatant was collected. The ethanol in the supernatant was recovered until no alcohol odor was detected. The supernatant was then freeze-dried to constant weight to obtain buckwheat seed extract.

[0026] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0027] Examples 1-5 The soothing and repairing compositions of Examples 1-5 were prepared according to the formulas shown in Table 1. The preparation method of the compositions is to mix the corresponding weight parts of bamboo fungus extract, dendrobium nobile extract, ginseng extract and buckwheat seed extract evenly to obtain the soothing and repairing compositions.

[0028] Table 1. Composition and dosage of the soothing and repairing composition.

[0029] Note: The total weight of the soothing and repairing compositions prepared in Examples 1-5 is the same.

[0030] Comparative Example 1 This comparative example provides a soothing and repairing composition, which differs from Example 1 only in that the soothing and repairing composition does not contain bamboo fungus extract, but instead uses Dendrobium nobile extract, Panax ginseng extract and buckwheat seed extract in a mass ratio of 4.8:1.6:1.6 to make up for the missing amount. The other parameters and steps are the same as in Example 1.

[0031] Comparative Example 2 This comparative example provides a soothing and repairing composition, which differs from Example 1 only in that the soothing and repairing composition does not contain Dendrobium nobile extract, but instead uses extracts of Dictamnus dasycarpus, Panax ginseng, and buckwheat seed extract in a mass ratio of 6:1.6:1.6 to make up for the missing amount. The remaining parameters and steps are the same as in Example 1.

[0032] Comparative Example 3 This comparative example provides a soothing and repairing composition, which differs from Example 1 only in that the soothing and repairing composition does not contain ginseng extract, but instead uses extracts of bamboo fungus, dendrobium nobile, and buckwheat seed in a mass ratio of 6:4.8:1.6 to make up for the missing amount. The remaining parameters and steps are the same as in Example 1.

[0033] Comparative Example 4 This comparative example provides a soothing and repairing composition, which differs from Example 1 only in that the soothing and repairing composition does not contain buckwheat seed extract, but instead uses extracts of Dictamnus longifolia, Dendrobium nobile, and Panax ginseng in a mass ratio of 6:4.8:1.6 to make up for the missing amount. The other parameters and steps are the same as in Example 1.

[0034] Comparative Example 5 This comparative example provides a soothing and repairing composition, which differs from Example 1 only in that the composition includes the following components in parts by weight: 1.6 parts of Bambusa textilis extract, 1.8 parts of Dendrobium nobile extract, 9 parts of Panax ginseng extract, and 1.6 parts of buckwheat seed extract. The remaining parameters and steps are the same as in Example 1.

[0035] In vitro efficacy verification of test cases 1. Hyaluronidase Inhibition Test Hyaluronic acid is an important component of the skin's extracellular matrix, playing a crucial role in maintaining its volume and normal skin physiological function. Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid and is associated with most IgE-mediated type I and T-cell-mediated type IV hypersensitivity reactions. Furthermore, the hydrolysis products of hyaluronic acid promote skin inflammation. Inhibiting hyaluronidase activity ensures normal hyaluronic acid content and function in the skin. Therefore, this invention characterizes the soothing efficacy of the soothing and repairing compositions of Examples 1-5 and Comparative Examples 1-5 by evaluating their inhibition rate of hyaluronidase activity. The test methods are as follows: Take 0.1 mL of 0.25 mmol / L CaCl2 aqueous solution and 0.5 mL of hyaluronidase solution (prepared with pH 5.6 acetate buffer, enzyme activity concentration of 1250 units / mL), mix and incubate at 37℃ for 20 min; add 0.5 mL of the test sample with a concentration of 1.0 mg / mL (the soothing and repairing compositions of Examples 1-5 and Comparative Examples 1-5, prepared with pH 5.6 acetate buffer), and continue incubation at 37℃ for 20 min, with three replicates for each sample; then add 0.5 mL of sodium hyaluronate solution (prepared with pH 5.6 acetate buffer, concentration of 0.5 mg / mL), incubate at 37℃ for 30 min, and let stand at room temperature for 5 min; add 0.1 mL of 0.4 mol / L... A mixture of NaOH aqueous solution and 0.5 mL of acetylacetone solution (prepared using 1.0 mol / L sodium carbonate solution, concentration 7% v / v) was heated in a boiling water bath for 15 min, followed immediately by cooling with ice water for 5 min. 1.0 mL of Ehrlich's reagent (prepared by dissolving 0.8 g of p-dimethylaminobenzaldehyde in 15 mL of 12 mol / L concentrated hydrochloric acid and 15 mL of anhydrous ethanol) was added, diluted with 3.0 mL of anhydrous ethanol, and allowed to stand for 20 min for color development. The absorbance was measured using a spectrophotometer, and the average value was taken. The hyaluronidase inhibition rate was calculated using the following formula: Hyaluronidase inhibition rate (%) = [(AB) - (CD)] / (AB); in: A: OD value of the control solution (using acetate buffer solution instead of sample solution); B: OD value of the control blank solution (using acetate buffer solution instead of sample solution and enzyme solution); C: OD value of the sample solution; D: OD value of blank sample solution (using acetate buffer solution instead of enzyme solution); The calculation results are shown in Table 2.

[0036] 2. Zebrafish neutrophil aggregation inhibition test Soothing refers to helping to improve skin irritation and other conditions. When the skin is stimulated by physical, chemical, or pathogenic invasion, it produces an immune response, resulting in discomfort such as redness and swelling, exhibiting signs of sensitive skin. At this time, immune leukocytes (including neutrophils and macrophages) aggregate in the skin to engulf and clear harmful substances such as infected, foreign bodies, or damaged cells. Inhibiting the aggregation of leukocytes at the irritated site can soothe skin irritation. Neutrophils in zebrafish embryos (the main immune leukocytes) are highly similar to human neutrophils in morphology, biochemistry, and physiological function. Therefore, this experiment used a model of neutrophil aggregation induced by copper sulfate stimulation of the lateral line region of zebrafish embryos. The changes in the number of neutrophils in the lateral line region of the test group and the model control group were compared, and the neutrophil aggregation inhibition rate was calculated to evaluate whether the soothing and repairing compositions of Examples 1-5 and Comparative Examples 1-5 have a soothing effect.

[0037] The testing method is as follows: Healthy zebrafish embryos, 3 days after fertilization, were selected and divided into a blank control group, a model control group, a positive control group, and a sample treatment group. The specific grouping settings are as follows: Blank control group setting: 24 fish embryos were randomly selected and placed into a 3cm culture dish, and 5mL of fish embryo culture medium was added.

[0038] Model control group setup: 24 fish embryos were randomly selected and placed in a 3cm culture dish, and 5mL of fish embryo culture medium containing 10µM anhydrous copper sulfate was added.

[0039] Positive control group setup: 24 fish embryos were randomly selected and placed in a 3cm culture dish, and 5mL of fish embryo culture medium containing 10µM anhydrous copper sulfate and 10µM indomethacin was added.

[0040] Sample processing group settings: Twenty-four fish embryos were randomly selected and placed into a 3cm culture dish. 5mL of fish embryo culture medium containing 10µM anhydrous copper sulfate and 0.5mg / mL of the test substance (the soothing and repairing composition of Examples 1-5 or Comparative Examples 1-5) was added.

[0041] All groups were incubated in a constant temperature incubator at 28℃±1℃ for 45 min.

[0042] Fish embryo fixation and staining: (1) After the culture was completed, each group of fish embryos was fixed in paraformaldehyde for at least 1 hour, then treated with PBST 3 times for 5 minutes each time, and then treated with 50% ethanol for 3 minutes. (2) After staining the fish embryos with Sudan Black staining solution at room temperature for 1 hour, they were washed with 70% ethanol 4 times for 5 minutes each time, and then treated with PBST 2 times for 5 minutes each time. (3) Treat fish embryos with bleaching solution for 10 min, then with 70% ethanol solution for 5 min, PBST for 1 min, clearing solution 1 for 15 min, clearing solution 2 for 10 min, and then with PBST for 3 min.

[0043] Sample analysis and data calculation: The fish embryos were placed on their sides. Then, the tail portion of the embryos was photographed under a stereomicroscope. The number of neutrophils in the three-quarters of the tail region from the anus was counted for each embryo, and the neutrophil aggregation inhibition rate was calculated. Inhibition rate = (MS) / (MB) × 100%; in: S—The average number of neutrophils in the embryos of fish in the test substance treatment group, expressed in units of neutrophils per fish (neutrophils / fish). M—The average number of neutrophils in the embryonic cells of fish in the model control group, expressed in units of neutrophils per fish (neutrophils / fish). B—The average number of neutrophils in the embryos of fish in the blank control group, in units of neutrophils per fish (neutrophils / fish). The calculation results are shown in Table 2.

[0044] Analysis of Table 2 shows that the soothing and repairing compositions provided in Examples 1-5 of this invention have significant hyaluronidase inhibition and neutrophil aggregation inhibition effects, indicating that the soothing and repairing compositions can reduce hyaluronic acid degradation and maintain the skin barrier structure by inhibiting hyaluronidase activity; at the same time, they can intervene in cellular immune responses, reduce skin irritation and inflammatory reactions, thereby achieving a soothing effect. Analysis of the results of Examples 1 and Comparative Examples 1-5 shows that the hyaluronidase inhibition rate and neutrophil aggregation inhibition rate of Comparative Examples 1-5 are far lower than those of Example 1. The difference between Comparative Examples 1-5 and Example 1 is that the soothing and repairing compositions lack any one of the following: *Dictamnus dasycarpus* extract, *Dendrobium nobile* extract, *Gynostemma pentaphyllum* extract, and *Buckwheat* seed extract, or the amount of these components is not within the limits specified in this invention. This indicates that the synergistic effect of the components in the soothing and repairing compositions has a significant impact on the ability to inhibit hyaluronidase and neutrophil aggregation. The absence of a single component or improper ratio of the components in the composition cannot achieve the same soothing effect.

[0045] Table 2 Results of in vitro efficacy tests Example 1 85.7 73.5 Example 2 78.1 65.9 Example 3 80.5 68.2 Example 4 81.2 71.5 Example 5 82.9 72.4 Comparative Example 1 56.8 48.1 Comparative Example 2 52.6 43.9 Comparative Example 3 63.4 52.8 Comparative Example 4 65.9 55.0 Comparative Example 5 70.2 57.3 Application Example 1 This application example provides a water-based cosmetic with soothing effects, which is prepared using the soothing and repairing compositions provided in Examples 1-5 using conventional techniques in the art. Specifically, the water-based cosmetic comprises the following components by weight percentage: 1.5% soothing and repairing composition, 50% glycerin, 0.6% 1,2-hexanediol, 0.2% caprylyl glycol, and the balance being deionized water.

[0046] Application Example 2 This application example uses the soothing and repairing compositions of Example 1 and Comparative Examples 1-5 to prepare nanoemulsions, resulting in nanoemulsion 1 and nanoemulsions 1'-5', respectively. Specifically, nanoemulsion 1 is prepared using the soothing and repairing composition of Example 1, nanoemulsion 1' is prepared using the soothing and repairing composition of Comparative Example 1, and so on, to obtain nanoemulsions 1'-5'. The nanoemulsions are prepared from the following raw materials in weight percentages: 1.5% soothing and repairing composition, 8% medium-chain triglycerides, 2.5% polyglycerol-6-laurate, 1.0% polyglycerol-4-oleate, 4.5% glycerol, 1.5% 1,2-hexanediol, 0.3% octyl glycol, and the balance being deionized water.

[0047] The preparation method of the nanoemulsion includes the following steps: S1. Medium-chain triglycerides, polyglycerol-6-laurate, polyglycerol-4-oleate, 1,2-hexanediol and octyl glycol are stirred and mixed evenly at 65°C and 300 r / min to obtain phase A; S2. Mix glycerol and 2 / 3 of the deionized water at 65°C and 300 r / min until homogeneous to obtain phase B; S3. Mix the soothing and repairing composition and the remaining deionized water at 40°C and 300 r / min until homogeneous to obtain phase C; S4. Slowly add phase B to phase A, stir and mix at 500 r / min for 25 min, cool to 40℃, add phase C, and then homogenize under high pressure 4 times at a pressure of 900 bar / min and a homogenization speed of 8000 r / min to obtain nanoemulsion.

[0048] This application example also provides nanoemulsion 6', which differs from nanoemulsion 1 only in that polyglycerol-4 oleate is not added to the raw materials of nanoemulsion 6', but is supplemented with an equal amount of polyglycerol-4 isostearate to make up for its missing amount. The remaining steps and parameters are the same as those of nanoemulsion 1 to prepare nanoemulsion 6'.

[0049] This application example also provides nanoemulsion 7', which differs from nanoemulsion 1 only in that polyglycerol-6 laurate is not added to the raw materials of nanoemulsion 7', and its missing amount is made up with an equal amount of glycerol stearate citrate. The remaining steps and parameters are the same as those of nanoemulsion 1 to prepare nanoemulsion 7'.

[0050] This application example also provides nanoemulsion 8', which differs from nanoemulsion 1 only in that octyl glycol is not added to the raw materials of nanoemulsion 8', and its missing amount is made up with polyglycerol-6 laurate and polyglycerol-4 oleate in a mass ratio of 2.5:1. The remaining steps and parameters are the same as those of nanoemulsion 1 to prepare nanoemulsion 8'.

[0051] This application example also provides nanoemulsion 9', which differs from nanoemulsion 1 only in that nanoemulsion 9' is prepared from the following raw materials in weight percentage: 1.5% soothing and repairing composition, 8% medium-chain triglycerides, 0.5% polyglycerol-6-laurate, 3.0% polyglycerol-4-oleate, 4.5% glycerol, 1.5% 1,2-hexanediol, 0.3% octyl glycol, and the balance being deionized water; its preparation method is the same as that of nanoemulsion 1, thus obtaining nanoemulsion 9'.

[0052] Test Example 2: Human Efficacy Trial To verify that the nanoemulsion provided by the present invention has soothing properties, nanoemulsion 1 and nanoemulsion 1'-5' were used as test samples for lactic acid stinging test.

[0053] Test subjects: Individuals with sensitive skin who tested positive for lactic acid stinging (i.e., lactic acid stinging score ≥3), without serious systemic diseases, immune deficiencies or autoimmune diseases, and without having received facial skin treatments, cosmetic procedures or other behaviors that may affect the results; A total of 30 volunteers with sensitive skin who tested positive for lactic acid stinging were recruited and randomly divided into 6 groups of 5 people each, and test samples were randomly distributed for testing.

[0054] Evaluation method for positive lactic acid stinging reaction: Under constant temperature (21℃±2℃) and constant humidity (50%±10%), 50μL of lactic acid aqueous solution (10% by mass of lactic acid in the solution) was applied to the nasolabial fold and one cheek. Subjects were asked about their subjective symptoms at 2.5min and 5min respectively, and scored on a 4-point scale (0, 1, 2, and 3 points, where 0 points indicate no stinging sensation, 1 point indicates mild stinging sensation, 2 points indicate moderate stinging sensation, and 3 points indicate severe stinging sensation). The sum of the two scores (2.5min + 5min) is the lactic acid stinging reaction score. Subjects with a lactic acid stinging reaction score ≥3 are considered to have a positive lactic acid stinging reaction.

[0055] Test method: (1) No cosmetics or medicines should be used on the test site on the day of the test. After washing with clean water, dry the skin surface with a dry facial tissue. The test subject should sit quietly for 30 minutes in a constant temperature and humidity environment. During this period, the subject should not drink water or eat any food, keep relaxed, and avoid touching the face. (2) Use a pipette to draw 50 μL of lactic acid aqueous solution (the mass percentage of lactic acid in the lactic acid aqueous solution is 10%) and randomly drop it onto one side of the filter paper, and add 50 μL of distilled water to the other side; place the two filter paper pieces symmetrically on the nasolabial groove of the test subject; ask the subject about their subjective symptoms at 2.5 min and 5 min respectively, and score them according to the 4-point method (the score is 0, 1, 2 and 3 points, where: 0 points indicates no stinging sensation, 1 point indicates mild stinging sensation, 2 points indicates moderate stinging sensation, and 3 points indicates severe stinging sensation). The sum of the scores of the two scores (2.5 min + 5 min) is the lactic acid stinging score. If the subject cannot tolerate the test during the test, the test material on both sides can be removed, and the score at the remaining time points is consistent with the score when the test material is removed.

[0056] (3) Evaluation of immediate soothing effect: After removing and cleaning the test sample from both sides, the test subject took 0.5 mL of the sample and applied it evenly to the nasolabial fold area. After massaging until fully absorbed, the test subject scored the sample after 30 minutes. The test subject evaluated the sample’s effect on relieving skin burning, stinging, dryness and itching and skin redness through questionnaires and inquiries. The scoring criteria are shown in Table 3 below. The higher the score, the better the test subject thinks the sample has immediate soothing effect.

[0057] Table 3. Scoring Criteria for Immediate Soothing Efficacy I strongly agree 7 I largely agree 6 Some agreement 5 Neither agreeing nor disagreeing 4 Some disagree 3 I don't quite agree 2 I strongly disagree 1 (4) Long-lasting soothing performance test: After cleansing, the test subject took 1.0 mL and applied it evenly to the face, massaged until fully absorbed, used once in the morning and once in the evening, for a total of two times a day, and did not use other skin care products or products with similar effects during the period; after 28 days of continuous use of the product, a follow-up visit was conducted, and steps (1)-(2) were repeated to conduct a lactic acid stinging test. The lactic acid stinging score of each test subject after 28 days of continuous use of the product was recorded, the average value of the results was taken, and the lactic acid stinging improvement rate was calculated.

[0058] The formula for calculating the improvement rate of lactic acid stinging is as follows: Improvement rate of lactic acid stinging (%) = (D28-D0) / D0×100%; Wherein, D0 is the lactic acid stinging score before using nanoemulsion, and D28 is the lactic acid stinging score 28 days after using nanoemulsion; The calculation results are shown in Table 4.

[0059] As shown in Table 4, the nanoemulsion 1 provided by this invention achieved an immediate soothing efficacy score of 6.6, indicating that the subjects generally agreed with the immediate soothing effect of nanoemulsion 1. Furthermore, after 4 weeks of use, the improvement rate of lactic acid stinging reached 85.71%, demonstrating that the nanoemulsion 1 and the soothing and repairing composition provided by this invention have good immediate soothing effects and significant long-term soothing and repairing effects. Comparing the data of nanoemulsion 1'-5' with nanoemulsion 1 reveals that the composition and dosage ratio of the soothing and repairing composition have a significant impact on the immediate and long-term soothing and repairing abilities of the composition and the prepared nanoemulsion.

[0060] Table 4 Results of Human Efficacy Trials Nanoemulsion 1 6.6 85.71% Nanoemulsion 1' 3.0 50.00% Nanoemulsion 2' 2.4 40.91% Nanoemulsion 3' 3.4 54.55% Nanoemulsion 4' 3.8 59.09% Nanoemulsion 5' 4.4 65.22% Test Example 3 To verify the stability of nanoemulsions 1 and 6'-9' prepared in this invention, their stability was evaluated through high-temperature, low-temperature, and centrifugal stability tests. The specific methods are as follows: High temperature test: Nanoemulsion 1 and nanoemulsion 6'-9' were placed in a suitable clean container and placed at 55℃ for 10 days. Samples were taken on the 10th day to observe the stratification of the samples.

[0061] Low temperature test: Nanoemulsion 1 and nanoemulsion 6'-9' were placed in a suitable clean container and placed at -20℃ for 5 days. Samples were taken on the 5th day to observe the stratification of the samples.

[0062] Centrifugation stability: Nanoemulsion 1 and nanoemulsion 6'-9' were injected into centrifuge tubes to about 2 / 3 of their height, sealed, and then placed in an electric thermostatic incubator pre-adjusted to 38℃. After 1 hour, the tubes were immediately transferred to a centrifuge and centrifuged at 2000 r / min for 30 min. The layering of the samples was then observed.

[0063] The results are shown in Table 5. The data in Table 5 show that nanoemulsion 1 exhibits good high-temperature stability, low-temperature stability, and centrifugal stability, while nanoemulsions 6'-9' show lower stability than nanoemulsion 1. The difference lies in the fact that during the preparation of the nanoemulsions, the emulsifiers polyglycerol-6-laurate and polyglycerol-4-oleate were replaced by emulsifiers with similar effects or the co-emulsifier octyl glycol was not added. The results indicate that in the preparation of nanoemulsions, the emulsifier obtained by combining polyglycerol-6-laurate and polyglycerol-4-oleate, along with octyl glycol as a co-emulsifier, can synergistically improve the stability of the nanoemulsions.

[0064] Table 5 Stability test results Nanoemulsion 1 Unlayered Unlayered Unlayered Nanoemulsion 6' Unlayered Layering Layering Nanoemulsion 7' Layering Layering Unlayered Nanoemulsion 8' Layering Layering Layering Nanoemulsion 9' Layering Layering Layering Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A soothing and repairing composition, characterized in that, It includes the following components in parts by weight: 2-10 parts of bamboo fungus extract, 2.5-7 parts of dendrobium nobile extract, 0.5-8 parts of ginseng extract and 0.5-8 parts of buckwheat seed extract.

2. The soothing and repairing composition as described in claim 1, characterized in that, It includes the following components in parts by weight: 4-8 parts of Bambusa textilis extract, 4.5-5.5 parts of Dendrobium nobile extract, 1-6 parts of Panax ginseng extract, and 1-6 parts of buckwheat seed extract.

3. The use of the soothing and repairing composition according to claim 1 or 2 in the preparation of cosmetics.

4. The application as described in claim 3, characterized in that, The amount of soothing and repairing composition added to the cosmetic is 0.5wt%-5wt%.

5. The application as described in claim 3, characterized in that, The dosage form of the cosmetic is selected from water, emulsion, cream, gel or mask.

6. A water-based cosmetic product, characterized in that, The composition comprises the following components by weight percentage: 0.5%-10% of the soothing and repairing composition according to any one of claims 1-3, 45%-55% of glycerin, 0.5%-0.6% of 1,2-hexanediol, 0.1%-0.5% of caprylyl glycol, and the balance being deionized water.

7. A nanoemulsion cosmetic, characterized in that, It is prepared from the following components in weight percentage: 0.5%-5% of the soothing and repairing composition according to any one of claims 1-3, 5%-10% of medium-chain triglycerides, 2%-3% of polyglycerol-6 laurate, 0.9%-1.2% of polyglycerol-4 oleate, 3%-6% of glycerol, 0.5%-5% of 1,2-hexanediol, 0.1%-0.5% of caprylyl glycol, and the balance being deionized water.

8. The nanoemulsion cosmetic according to claim 7, characterized in that, The preparation method of the nanoemulsion cosmetic includes the following steps: S1. Medium-chain triglycerides, polyglycerol-6-laurate, polyglycerol-4-oleate, 1,2-hexanediol and octyl glycol are mixed evenly to obtain phase A; S2. Mix glycerin and two-thirds of the amount of deionized water thoroughly to obtain phase B; S3. Mix the soothing and repairing composition with the remaining deionized water until homogeneous to obtain phase C; S4. Slowly add phase B to phase A, stir and mix, then add phase C, homogenize under high pressure to obtain nanoemulsion.

9. The nanoemulsion cosmetic as described in claim 8, characterized in that, The mixing conditions described in step S4 are: rotation speed 300-600 r / min, time 20-30 min.

10. The nanoemulsion cosmetic as described in claim 8, characterized in that, The conditions for high-pressure homogenization described in S4 are: pressure 800-1000 bar / min, homogenization speed 4000-10000 r / min, temperature 35-45℃, and number of cycles 3-5.

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