Application of TaWIP2 protein and coding gene in improvement of genetic transformation efficiency and yield of wheat

By overexpressing the TaWIP2 protein and gene in wheat, the problems of low genetic transformation efficiency and unstable yield traits in wheat have been solved, achieving efficient genetic transformation and yield improvement, and providing new gene resources for wheat functional research and breeding.

CN122444844AActive Publication Date: 2026-07-24SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610902185.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-23
Publication Date
2026-07-24
Estimated Expiration
2046-06-23

AI Technical Summary

Technical Problem

Wheat genetic transformation efficiency is low, genotype dependence is strong, and the scope of application of transformation systems is narrow. The expression of existing regenerated genes is uncontrolled and can easily affect yield traits, making it difficult to meet the needs of high-throughput functional verification and precision breeding.

Method used

Using the TaWIP2 protein and its encoding gene, a recombinant expression vector was constructed to overexpress the TaWIP2 gene. This gene was then introduced into wheat embryos via Agrobacterium-mediated transformation to improve genetic transformation efficiency and increase yield by regulating grain length, grain width, and thousand-grain weight.

Benefits of technology

It significantly improves the efficiency of wheat genetic transformation, while also improving grain traits and increasing yield, providing a stable and efficient genetic transformation tool suitable for wheat gene function research and molecular breeding.

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Abstract

The invention discloses application of TaWIP2 protein and a coding gene in improvement of genetic transformation efficiency and yield of wheat, and belongs to the technical field of plant genetic engineering. The amino acid sequence of the TaWIP2 protein disclosed by the invention is as shown in SEQ ID NO.2, and the nucleotide sequence of the TaWIP2 protein coding gene is as shown in SEQ ID NO.1; a novel TaWIP2 protein participating in regulation and control of wheat regeneration and a coding gene thereof are found in a wheat genome, and the TaWIP2 gene is overexpressed, so that the wheat genetic transformation efficiency is improved, and meanwhile, the yield of wheat can be increased; and a new gene resource is provided for breaking the bottleneck of wheat genetic transformation, accelerating wheat gene function research and improving crop agronomic traits.
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