Domestication method, separation and purification method and application of low-temperature-resistant straw degrading bacteria

By using exogenous amino acid induction and cyclic acclimatization methods, the problem of low straw degradation efficiency at low temperatures was solved, and highly efficient cold-resistant strains were screened out, achieving efficient straw degradation under low-temperature conditions.

CN122445480APending Publication Date: 2026-07-24SHANDONG UNIV OF TECH +1
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Patent Information

Application Number
CN202610764163.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-29
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies struggle to efficiently degrade straw under low-temperature conditions. Traditional methods suffer from long screening cycles, limited degradation activity, poor environmental adaptability, and high technical barriers.

Method used

By combining exogenous amino acid induction with systematic cyclic acclimatization, the low-temperature adaptability and degradation activity of the microbial community were enhanced by gradually reducing the temperature, decreasing the amino acid concentration, and introducing periodic temperature fluctuations.

Benefits of technology

The breeding cycle was significantly shortened, and cold-resistant strains with strong genetic stability were screened out. These strains have significant low-temperature degradation capabilities and improve straw degradation efficiency.

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Abstract

The invention discloses a domestication method, a separation and purification method and application of low-temperature-resistant straw degrading bacteria, and belongs to the field of agricultural microbial technology and waste resource utilization. The domestication method specifically comprises the steps of bacteria source sample preparation, straw pretreatment, culture medium preparation and multi-round low-temperature domestication culture; in the process, after each round of domestication, the flora degradation efficiency is detected by taking the straw weight loss ratio as an index, and the obtained low-temperature-resistant straw degrading bacterium is preserved in the China Center for Type Culture Collection on April 27, 2026, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province; the preservation number is CCTCC (China Center For Type Culture Collection) NO: The low-temperature-resistant straw degrading bacterium is named as aureobasidium pullulans X4-8 (Aureobasidium sp. X4-8) according to taxonomy, and the low-temperature-resistant straw degrading bacterium is named as aureobasidium pullulans sp. X4-8. According to the domestication method, exogenous amino acid induction and systematic recurrent domestication are combined, and the low-temperature adaptability and degradation activity of the microbial flora are actively enhanced. In addition, the invention further discloses a separation and purification method and application of the low-temperature-resistant straw degrading bacteria.
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