Pseudomonas putida recombinant bacterium as well as construction method and application thereof

By knocking out the PP_3425 and PP_3283 genes of *Pseudomonas putida* KT2440 and overexpressing the vanAB gene, a recombinant strain was constructed, which solved the problem of insufficient stability of KT2440 in high-concentration aromatic substrate environments, and achieved a significant increase in PHA yield and concentration, meeting industrial needs.

CN122445683APending Publication Date: 2026-07-24TIANJIN UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202610722419.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-25
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

The existing P. odorifera KT2440 catalyzes the production of PHA from lignin derivatives, but the PHA yield is insufficient to meet industrial requirements, and it lacks stability and has a limited tolerance threshold in high-concentration aromatic substrate environments.

Method used

By knocking out the PP_3425 and PP_3283 genes of *Pseudomonas putida* KT2440 and overexpressing the vanAB gene, a recombinant strain was constructed, enhancing its tolerance to high concentrations of lignin derivatives and its ability to synthesize PHA.

Benefits of technology

It significantly improved the growth stability and PHA synthesis efficiency of the recombinant strain under high concentrations of lignin derivatives, increasing PHA yield by approximately 46.4% and concentration by approximately 78.3%, meeting industrial requirements.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a pseudomonas putida recombinant bacterium as well as a construction method and application thereof. The construction method comprises the following steps: (1) knocking out a PP3425 gene and a PP3283 gene of pseudomonas putida KT2440; (2) overexpressing the vanAB gene, the lignin derivative is used as a main carbon source, the cost of the raw material is obviously reduced, and the source is green and renewable; the recombinant bacterium is excellent in performance, and the PHA yield is remarkably increased. The constructed recombinant bacterium not only retains endogenous efficient PHA synthesis capability, natural stress resistance and growth robustness of the wild bacterium KT2440, but also remarkably improves the growth stability and PHA synthesis efficiency under a high-concentration lignin derivative. Experiments prove that the PHA content of the recombinant bacteria is increased by about 46.4% compared with that of the wild bacteria, and the PHA concentration is increased by about 78.3%, so that the intracellular synthesis capability and the total fermentation yield of the recombinant bacteria are greatly improved.
Need to check novelty before this filing date? Find Prior Art