Method for improving low-temperature resistance of tomato by knocking out SlSWC6 gene and application

By knocking out the SlSWC6 gene in tomato using CRISPR/Cas9 gene editing technology, the problem of tomato's sensitivity to low-temperature stress was solved, its low-temperature tolerance was improved, and the research gap of SWR1 complex in low-temperature stress was filled. This provides gene resources and theoretical basis for breeding new low-temperature tolerant varieties.

CN122445707APending Publication Date: 2026-07-24ZHEJIANG UNIV
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Patent Information

Application Number
CN202610877400.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-17
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Tomatoes are sensitive to low temperature stress, which leads to slow growth, delayed development and reduced yield. The mechanism of action of the SWR1 complex and its subunits in low temperature stress has not been systematically reported in the existing technology, especially the lack of functional studies in tomatoes.

Method used

By knocking out the SlSWC6 gene in tomatoes using CRISPR/Cas9 gene editing technology, cold-resistant transgenic plants were constructed using gene knockout technology. sgRNA targeting SlSWC6 was designed and introduced into a CRISPR/Cas9 vector. Mutants were screened and verified to obtain new cold-resistant tomato varieties.

Benefits of technology

It significantly improved the low-temperature resistance of tomatoes, reduced the inhibition of photosynthesis by low-temperature stress, protected the activity of photosystem II, reduced leaf cell membrane damage, and enhanced the low-temperature tolerance of tomatoes.

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Abstract

The invention discloses a method for improving tomato low-temperature resistance by knocking out an SlSWC6 gene and application, and belongs to the technical field of biology. The nucleotide sequence of the protein coding region of the SlSWC6 gene is as shown in SEQ ID NO: 1. According to the invention, the SlSWC6 gene is knocked out by virtue of a CRISPR / Cas9 gene knockout technology, so that the resistance of tomatoes to low-temperature stress can be remarkably improved. A low-temperature treatment experiment result shows that the SlSWC6 gene knockout strain shows higher survival rate, lower relative electrolyte permeability and higher photochemical efficiency at low temperature, and proves that the gene plays a negative regulation role in tomato low-temperature response. The invention not only provides an important gene resource for cultivating a new variety of low-temperature-resistant tomatoes, but also lays a theoretical foundation for analyzing a low-temperature stress response mechanism of the tomatoes.
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