Method for extracting and detecting matrine drugs in tea leaves

By preparing polydopamine-modified magnetic nanomaterials (Fe3O4-PDA) as adsorbents, and combining magnetic solid-phase extraction and liquid chromatography-tandem mass spectrometry, the selectivity and sensitivity issues of matrine-like drugs in tea were solved, realizing rapid, simple, and efficient extraction and detection of matrine-like drugs in tea.

CN122449022APending Publication Date: 2026-07-24SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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Patent Information

Application Number
CN202610754394.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-28
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies for detecting matrine-like drugs in tea suffer from problems such as low selectivity in pretreatment, poor purification effect, cumbersome operation, long time consumption, large amount of organic solvent used, and low sensitivity, making it difficult to meet the requirements of tea quality and safety and export testing.

Method used

Polydopamine-modified magnetic nanomaterials (Fe3O4-PDA) were used as magnetic solid-phase extraction adsorbents. Matrine-like drugs were selectively adsorbed through electrostatic interactions and π-π conjugation. Impurities were rapidly separated by magnetic separation technology. The target analytes were eluted with alkaline methanol solution and detected by liquid chromatography-tandem mass spectrometry.

Benefits of technology

This method achieves highly selective extraction and purification of matrine-like drugs in tea, significantly reduces matrix effects, simplifies the operation process, shortens pretreatment time, reduces the amount of organic solvent used, improves detection sensitivity, and meets EU limits.

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Abstract

The invention belongs to the technical field of analysis and detection, and particularly relates to a method for extracting and detecting matrine drugs in tea leaves. Firstly, a magnetic nano material (FeO-PDA) with the surface modified with polydopamine is prepared to serve as an adsorbent, then the material is mixed with a to-be-detected tea sample extracted through an acidic aqueous solution, the magnetic nano material selectively adsorbs matrine drugs in the sample under the neutral and meta-acid condition with the pH being 6.5, then the magnetic material is rapidly separated through an external magnetic field, and the matrine drugs in the to-be-detected tea sample are detected. Removing uncombined impurities such as pigments and polyphenols; and eluting the matrine target object combined with the material by using an alkaline methanol solution, and filtering the eluent. Based on the electrostatic interaction and the pi-pi conjugation effect of the polydopamine and the matrine drugs, rapid and selective extraction, enrichment and analysis of the matrine drugs in the complex tea matrix are achieved, and the method has the advantages of being easy and convenient to operate, short in time consumption, high in sensitivity, capable of achieving batch preparation of materials and the like.
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