A strain of gomphidius viscidus du657, culture method thereof and application thereof in preventing and treating plant pathogenic fungi

By isolating and culturing the strain Du657 of Pleurotus ostreatus, its polyphenol extract was prepared as a biocontrol agent, which solved the problems of drug resistance and pollution caused by chemical fungicides and achieved efficient and environmentally friendly inhibition of plant pathogens.

CN122628890APending Publication Date: 2026-08-25YANGTZE NORMAL UNIVERSITY
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Patent Information

Application Number
CN202610872630.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-16
Publication Date
2026-08-25

AI Technical Summary

Technical Problem

The current agricultural production relies on chemical fungicides to control plant pathogens, leading to pathogen resistance, pesticide residues, and environmental pollution. Furthermore, the application of Pleurotus ostreatus strains in agricultural biocontrol is currently lacking.

Method used

The strain Du657 of Pleurotus ostreatus was isolated and purified, and biocontrol agents were prepared using its secondary metabolites through specific culture methods and polyphenol extraction technology to inhibit plant pathogenic fungi.

Benefits of technology

It provides highly effective inhibition of plant pathogens such as Fusarium graminearum, Polychaete spp. palmis, and Cladosporium spp., is environmentally friendly, easily degradable, has no toxic side effects, reduces the use of chemical pesticides, and is in line with sustainable development.

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Abstract

The application discloses a Pleurotus cystidiosus strain Du657, a culture method thereof and application of the strain in preventing and treating plant pathogenic fungi. The Pleurotus cystidiosus strain Du657 has been preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.42785. The application is based on morphological and molecular biological identification, and a new strain with excellent growth traits and strong bacteriostatic activity is screened from wild resources. An optimized mother culture, original culture and cultivation medium formula for the strain are provided, and efficient artificial domestication of the strain is successfully realized. Experiments prove that a fruit body polyphenol extract of the Du657 strain has a significant inhibitory effect on a plurality of plant pathogenic fungi such as wheat scab fungus and Pestalotiopsis versicolor. The application greatly enriches fungal germplasm resources with biocontrol potential, and provides a source and material basis for development of a green, efficient and pollution-free natural fungal source biological fungicide. The application has a high application value and broad prospects in the field of modern agricultural disease green prevention and control.
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