Purification device

Abstract

The utility model provides a purification device. The purification device comprises a container for accommodating a dialysis bag, a monitoring assembly, a liquid replacement assembly and a control assembly, the container is filled with liquid, and the dialysis bag is soaked in the liquid and is connected with the container; at least part of the monitoring assembly is located in the container, and the monitoring assembly is used for monitoring at least one performance parameter of liquid in the container in real time; the liquid replacement assembly is connected with the container; the control assembly is electrically connected with the monitoring assembly and the liquid replacing assembly and used for controlling the liquid replacing assembly to replace the liquid in the container according to the at least one performance parameter. The purification device provided by the utility model can improve the purification efficiency.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological purification, and in particular to a purification device. Background Art

[0002] Dialysis is one of the commonly used separation and purification techniques in the field of biochemistry. In the preparation of biomacromolecules, dialysis is used to remove salts, remove small amounts of organic solvents, remove small biological molecule impurities, and concentrate samples.

[0003] The dialysis process is carried out by using a purification device. The purification device includes a dialysis bag made of a semipermeable membrane and a container filled with a liquid (usually water or a buffer solution). The semipermeable membrane has pores of a certain diameter. When the molecular diameter (determined by the molecular weight) is larger than the pore diameter, the molecule cannot pass through the semipermeable membrane, but when it is smaller, it can pass freely. During dialysis, the dialysis bag is placed in the container. Under the action of diffusion pressure, molecules have the characteristic of spontaneously diffusing from high concentration to low concentration. Small molecules (impurities) will continue to diffuse into water or buffer through the semipermeable membrane until the concentration of the small molecules inside and outside the dialysis bag is equal, while the large molecules (target) are left in the dialysis bag, so that the liquid in the dialysis bag is purified. During dialysis, in order to achieve a better dialysis effect, the liquid outside the dialysis bag (dialysis solution) needs to be replaced regularly. The replacement time, number of replacements, and frequency of the dialysis solution will ultimately affect the dialysis effect.

[0004] However, when the above purification device is used to purify the sample, the operator determines when the liquid in the container needs to be replaced based on his or her own experience. When the operator believes that the liquid in the container needs to be replaced, the liquid in the container is manually drained and then new liquid is added. It can be seen that the above operation is completely dependent on manual labor, which is not only inefficient, but also very subjective to judge whether the liquid needs to be replaced based on experience. It is also easy to cause misjudgment due to differences between individuals, thereby affecting the purification efficiency and effect. Utility Model Content

[0005] The embodiment of the present disclosure provides a purification device that can improve purification efficiency. The technical solution is as follows:

[0006] An embodiment of the present disclosure provides a purification device, which includes a container for accommodating a dialysis bag, a monitoring component, a liquid replacement component and a control component; the container is filled with a liquid, the dialysis bag is immersed in the liquid and is connected to the container; at least a portion of the monitoring component is located in the container, and is used to monitor at least one performance parameter of the liquid in the container in real time; the liquid replacement component is connected to the container; the control component is electrically connected to the monitoring component and the liquid replacement component, respectively, and is used to control the liquid replacement component to replace the liquid in the container according to the at least one performance parameter.

[0007] In another implementation of the present disclosure, the monitoring component includes at least one of the following sensors: a conductivity sensor, a total dissolved solids sensor, a temperature sensor, a pH sensor, and an ammonium ion count sensor.

[0008] In yet another implementation of the present disclosure, the monitoring component includes at least two sensors, and the at least two sensors are integrated into an integrated probe.

[0009] In another embodiment of the present disclosure, the container has a liquid inlet and a liquid outlet; the liquid replacement component includes a first drive pump and a second drive pump, the outlet of the first drive pump is connected to the liquid inlet, and the inlet of the first drive pump is connected to a new liquid device; the inlet of the second drive pump is connected to the liquid outlet, and the outlet of the second drive pump is connected to a waste liquid device, and the first drive pump and the second drive pump are both connected to the control component.

[0010] In another implementation of the present disclosure, the liquid replacement assembly also includes a first pipeline and a second pipeline, wherein the two ends of the first pipeline are respectively connected to the outlet of the first drive pump and the liquid inlet, and the two ends of the second pipeline are respectively connected to the inlet of the second drive pump and the liquid outlet.

[0011] In another implementation of the present disclosure, the control component includes multiple drive chips and a controller; the drive chips are connected to the sensors one by one; the multiple drive chips are all connected to the controller, and the controller is respectively connected to the first drive pump and the second drive pump.

[0012] In another implementation of the present disclosure, the purification device further includes a workbench, and the container is connected to the workbench; the first driving pump, the second driving pump, and the driving chip are all integrated in the workbench.

[0013] In another implementation of the present disclosure, the workbench includes a power interface and a USB interface, the power interface is electrically connected to the drive chip, the first drive pump, the second drive pump and the integrated probe, respectively; the USB interface is connected to the drive chip and is used to connect to the controller.

[0014] In yet another implementation of the present disclosure, the purification device further includes an agitator, and the agitator is located in the container.

[0015] In another implementation of the present disclosure, the purification device further includes a workbench having a placement plane, the bottom of the container is located on the placement plane, and the placement plane is a plane with variable magnetism when powered on; the agitator is a magnetic agitator.

[0016] The technical solution provided by the embodiments of the present disclosure has the following beneficial effects:

[0017] Since the purification device includes a container and a monitoring component, and at least a portion of the monitoring component is connected to the container and is used to monitor at least one performance parameter of the liquid in the container in real time, the liquid for purification can be contained in the container, and the performance of the liquid in the container can be monitored in real time by the monitoring component, so as to track the progress of dialysis in real time.

[0018] Furthermore, since the purification device further includes a liquid replacement component and a control component, and the liquid replacement component is connected to the container for replacing the liquid in the container, and the control component is electrically connected to the monitoring component and the liquid replacement component, respectively, for controlling the liquid replacement component to replace the liquid in the container when the monitoring result of the monitoring component is the target result, the liquid replacement component can be automatically controlled by the control component according to the monitoring result of the monitoring component, so as to automatically replace the liquid in the container during the purification process, and it can also avoid misjudgment caused by the conjecture of the supervisor, thereby greatly improving the purification efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 A simplified schematic diagram of a purification device provided in an embodiment of the present disclosure;

[0021] Figure 2 A schematic diagram of the operation principle of a purification device provided in an embodiment of the present disclosure.

[0022] The symbols in the figure mean the following:

[0023] 1. Container; 2. Monitoring component;

[0024] 3. Liquid replacement assembly; 31. First drive pump; 32. Second drive pump; 33. First pipeline; 34. Second pipeline;

[0025] 4. Control component; 41. Driver chip; 43. TTL conversion chip; 42. Controller;

[0026] 5. Workbench; 50. Placement surface; 51. Power interface; 52. USB interface; 6. Stirrer; 7. Dialysis bag. DETAILED DESCRIPTION

[0027] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the embodiments of the present disclosure will be further described in detail below with reference to the accompanying drawings.

[0028] The present disclosure provides a purification device, such as Figure 1 As shown, the purification device comprises a container 1 for accommodating a dialysis bag, a monitoring component 2, a liquid replacement component 3 and a control component 4. The container 1 is filled with liquid, and the dialysis bag is immersed in the liquid and connected to the container.

[0029] At least part of the monitoring component 2 is located in the container 1 and is used to monitor in real time at least one performance parameter of the liquid in the container 1. The liquid replacement component 3 is connected to the container 1.

[0030] The control component 4 is electrically connected to the monitoring component 2 and the liquid replacement component 3 respectively, and is used to control the liquid replacement component 3 to replace the liquid in the container 1 according to at least one performance parameter.

[0031] When the purification device provided by the embodiment of the present disclosure purifies the sample, since the dialysis bag is immersed in the liquid during the purification process, the composition of the liquid in the container 1 will continue to change as the purification process proceeds. The small molecules in the sample in the dialysis bag will continuously enter the liquid in the container 1, which will affect at least one of the optical properties, electrical properties, biological properties, and chemical properties of the liquid in the container 1 and cause changes. Therefore, by monitoring the changes in at least one performance parameter of the liquid in the container 1, the performance of the liquid in the container 1 can be fed back, thereby indirectly determining the progress of the purification process, that is, whether the liquid in the container 1 needs to be replaced or whether the purification is completed, or whether an accident (such as sample leakage) occurs.

[0032] Since the purification device includes a container 1 and a monitoring component 2, and at least a portion of the monitoring component 2 is connected to the container 1 and is used to monitor at least one performance parameter of the liquid in the container 1 in real time, the container 1 can be used to hold the liquid for purification, and at the same time, the monitoring component 2 can be used to monitor at least one performance parameter of the liquid in the container 1 in real time, so as to track the progress of purification in real time.

[0033] Furthermore, since the purification device further includes a liquid replacement component 3 and a control component 4, and the liquid replacement component 3 is connected to the container 1 for replacing the liquid in the container 1, and the control component 4 is electrically connected to the monitoring component 2 and the liquid replacement component 3 respectively, and is used to control the liquid replacement component 3 to replace the liquid in the container 1 when the monitoring result of the monitoring component 2 is the target result, the liquid replacement component 3 can be automatically controlled by the control component 4 according to the monitoring result of the monitoring component 2, so as to automatically replace the liquid in the container 1 during the purification process. Moreover, it can also avoid misjudgment caused by the assumption of the supervisor, and greatly improve the purification efficiency.

[0034] Optionally, the monitoring component 2 includes at least one of the following sensors: a conductivity sensor, a total dissolved solids sensor, a temperature sensor, a pH value sensor, and an ammonium ion count sensor.

[0035] By setting different types of sensors, different performance parameters of the liquid can be easily monitored. For example, if you need to monitor the temperature, you can use a temperature sensor.

[0036] Optionally, the monitoring component 2 includes at least two sensors, and the at least two sensors are integrated into an integrated probe.

[0037] In the above implementation, the monitoring component 2 is configured as an integrated probe, so that multiple performance parameters of the liquid in the container 1 can be monitored simultaneously, and the structure, connection method, wiring, etc. of the monitoring component 2 can be simplified.

[0038] In this embodiment, the above purification device is generally used in a desalination purification process. Correspondingly, the performance parameters monitored by the monitoring component 2 may be electrical conductivity (EC) and total dissolved solids (TDS).

[0039] In the desalting and purification process, as the purification process proceeds, salt will gradually precipitate out of the sample. The more salt precipitated in the sample, the higher the salinity of the liquid in container 1, and the higher the EC and TDS of the liquid in container 1. On the contrary, if the EC and TDS of the liquid in container 1 enter a plateau phase (the so-called plateau phase means that it is no longer increasing but in a state of equilibrium), and no longer changes with time, that is, no salt ions will be precipitated in the sample, and the salt ions in the sample and the salt ions in the liquid are saturated, then the purification can be terminated or the water can be further changed.

[0040] Of course, the performance parameters monitored by the monitoring component 2 completely depend on the specific purification process. For example, the performance parameters monitored by the monitoring component 2 can be optical parameters, such as absorbance, etc. At this time, the sample liquid can be a nucleic acid to be purified, etc. If the nucleic acid sample in the dialysis bag leaks, there will be macromolecules such as nucleic acids or proteins in the liquid in the container 1. ODA260 and A280, as characteristic peaks of nucleic acids and proteins, will have their readings rising rapidly, that is, the values ​​of the performance parameters monitored in the monitoring component 2 will increase significantly.

[0041] Optionally, the container 1 has a liquid inlet and a liquid outlet. The liquid replacement assembly 3 includes a first drive pump 31 and a second drive pump 32, the outlet of the first drive pump 31 is connected to the liquid inlet, and the inlet of the first drive pump 31 is connected to the new liquid device (not shown in the figure).

[0042] The inlet of the second driving pump 32 is connected to the liquid outlet, the outlet of the second driving pump 32 is connected to the waste liquid device (not shown in the figure), and the first driving pump 31 and the second driving pump 32 are both connected to the control component 4.

[0043] In the above implementation, the liquid replacement component 3 is configured as the above structure, so that liquid can be stored in the container 1 through the first driving pump 31 , and the liquid in the container 1 can be quickly discharged through the second driving pump 32 .

[0044] That is, when the liquid in the container 1 needs to be replaced, the second driving pump 32 is started to discharge the liquid in the container 1. After the discharge is completed, the first driving pump 31 is started to store liquid in the container 1. In this way, the liquid in the container 1 can be automatically replaced.

[0045] In this embodiment, the first driving pump 31 and the second driving pump 32 are both peristaltic pumps, which are easy to operate, have low noise, and low maintenance costs.

[0046] Optionally, the liquid replacement assembly 3 also includes a first pipeline 33 and a second pipeline 34, the two ends of the first pipeline 33 are respectively connected to the outlet and the liquid inlet of the first drive pump 31, and the two ends of the second pipeline 34 are respectively connected to the inlet and the liquid outlet of the second drive pump 32.

[0047] In the above implementation, the first pipeline 33 is used to connect the outlet of the first driving pump 31 and the liquid inlet in the container 1 , and the second pipeline 34 is used to connect the inlet of the second driving pump 32 and the liquid outlet in the container 1 .

[0048] In this embodiment, the first pipeline 33 and the second pipeline 34 are both hoses, which are convenient for bending and arrangement.

[0049] Of course, the first pipeline 33 and the second pipeline 34 may also be pipelines directly integrated on the first drive pump 31 and the second drive pump 32. As long as the outlet of the first drive pump 31 and the liquid inlet in the container 1 can be connected, and the inlet of the second drive pump 32 and the liquid outlet in the container 1 can be connected, the present disclosure does not limit the arrangement of the first pipeline 33 and the second pipeline 34.

[0050] Optionally, the control component 4 includes a plurality of drive chips 41 and a controller 42, wherein the plurality of drive chips 41 are connected to the plurality of sensors one by one, the plurality of drive chips 41 are connected to the controller 42, and the controller 42 is connected to the first drive pump 31 and the second drive pump 32 respectively.

[0051] In the above implementation, the driver chip 41 is used to control the operating status of the corresponding sensor (such as switching, adjusting the main voltage to the working voltage, adjusting the main frequency to the working frequency, setting the working mode and parameters of the sensor, etc.), and at the same time read and process the data monitored by the corresponding connected sensor (such as converting analog signals into digital signals, caching data, and ensuring data integrity).

[0052] The controller 42 is used to automatically control the operating states of the first driving pump 31 and the second driving pump 32 according to the data monitored by the integrated probe.

[0053] like Figure 2 As shown, in this embodiment, in order to facilitate the connection between the controller 42 and the driving chip 41, the control component 4 may further include a TTL (Transistor-Transistor-Logic) conversion chip 43. The conversion chip 43 is connected to the driving chip 41 and the controller 42 respectively.

[0054] The TTL conversion chip 43 is used to collect the data processed by the driver chip 41 , and transmit it to the controller 42 after arranging it into the format of the USB protocol.

[0055] In this embodiment, the controller 42 can be a single chip microcomputer, a mobile phone, a computer, etc. equipped with programming software. The controller 42 is pre-set with a target value corresponding to the performance parameter monitored by the integrated probe. When the performance parameter monitored by the integrated probe is not greater than the target value, it means that the purification process can be ended. At this time, the controller 42 can automatically drain the water in the container 1.

[0056] Of course, in order to avoid accidents, a threshold value corresponding to the performance parameter monitored by the integrated probe may also be provided inside the controller 42. When the performance parameter monitored by the integrated probe is higher than the threshold value, it means that an accident has occurred in the purification process, such as a failure of the integrated probe or a leakage of the sample. At this time, the purification device may be rechecked. When the performance parameter monitored by the integrated probe is higher than the threshold value, the controller 42 may automatically issue a corresponding prompt, such as sending an error report or outputting a signal, etc. as a reminder.

[0057] In order to facilitate understanding of the entire progress of the purification process, a purification report can be automatically generated by the controller 42 after the purification is completed. The report content includes the change process of the monitored performance parameters and the time of this purification process.

[0058] See again Figure 1 Optionally, the purification device further comprises a workbench 5, and the container 1 is connected to the workbench 5. The first driving pump 31, the second driving pump 32, and the driving chip 41 are all integrated in the workbench 5.

[0059] In the above implementation, the workbench 5 is arranged, which not only facilitates the arrangement and fixation of the container 1, but also allows the first driving pump 31, the second driving pump 32, the driving chip 41, etc. to be integrated into the workbench 5, so as to simplify the structure of the purification device.

[0060] Of course, the first driving pump 31 and the second driving pump 32 may not be integrated into the workbench 5 , but may be located only on one side of the workbench 5 .

[0061] Optionally, the workbench 5 includes a power interface 51 and a USB interface 52, the power interface 51 is electrically connected to the drive chip 41, the first drive pump 31, the second drive pump 32 and the integrated probe, respectively. The USB interface 52 is connected to the drive chip 41 and is used to connect to the controller 42.

[0062] In the above implementation, the power interface 51 is used to connect to an external power source to supply power to the driver chip 41 , the first drive pump 31 , the second drive pump 32 and the integrated probe, etc. The USB interface 52 is used to transmit the data stored and processed by the driver chip 41 to the controller 42 .

[0063] Optionally, the workbench 5 has a placement plane 50 , and the bottom of the container 1 is located on the placement plane 50 .

[0064] A placement plane 50 is arranged on the workbench 5, so that the container 1 can be directly fixed, avoiding the use of other fixing devices to limit the container 1.

[0065] Of course, the workbench 5 may also be provided with other limiting structures to place the container 1, such as a limiting groove, and the container 1 is located in the limiting groove.

[0066] Optionally, a battery is embedded in the workbench 5 , and the battery is electrically connected to the first driving pump 31 , the second driving pump 32 and the driving chip 41 , respectively.

[0067] In the above implementation, a battery is arranged in the workbench 5, so that the first drive pump 31, the second drive pump 32 and the drive chip 41 can be directly powered by the battery, so that the first drive pump 31, the second drive pump 32 and the monitoring component 2 can still work directly without an external power supply.

[0068] Optionally, the purification device further comprises a stirrer 6 , and the stirrer 6 is located in the container 1 .

[0069] In the above implementation, the arrangement of the stirrer 6 can stir the liquid in the container 1 to accelerate the dialysis efficiency.

[0070] In this embodiment, the stirrer 6 is a magnetic stirrer, and the placement plane 50 is a plane with variable magnetism when powered on.

[0071] Of course, the stirrer 6 can also be a mechanical stirrer.

[0072] In addition, in order to adapt to the purification of different samples, the placement plane 50 can also be a plane that can be heated when powered on, so that the liquid in the container 1 can be heated.

[0073] In this embodiment, the dialysis bag 7 is formed by a semipermeable membrane. The sample to be dialyzed is contained in the dialysis bag 7, and the dialysis bag 7 is fixedly connected to the inner wall of the container 1 through a fixing frame, etc. This makes it convenient to connect the dialysis bag 7.

[0074] In this embodiment, the container 1 can be a beaker, and the size of the beaker is selected according to actual needs. According to the requirements of dialysis, the volume of the liquid in the container 1 is at least 100 times the volume of the sample. For example, 10 ml of sample liquid is dialyzed with 1 L of liquid (such as water).

[0075] The following is a brief introduction to the working process of the purification device provided in the embodiment of the present disclosure:

[0076] The controller 42 controls the first driving pump 31 to store liquid in the container 1 , so that a fixed amount (generally 1 L) of liquid is contained in the container 1 .

[0077] The controller 42 controls the driving chip 41 so that the integrated probe starts monitoring. The dialysis bag 7 is placed in the container 1. The controller 42 starts the agitator 6 to start running. The integrated probe reads at least one performance parameter (such as TDS and conductivity) of the liquid in the container 1 in real time.

[0078] When the physical variables of the monitored performance parameters do not change within 15 minutes, it will be determined as purification balance and the water change process will be started. The agitator 6 stops working. Start the second drive pump 32 to start drainage. After the drainage is completed, start the first drive pump 31 to re-add a certain amount of new liquid to the container 1. If the performance parameters of the newly added liquid are abnormal, an early warning email will also be issued. When the monitored performance parameters are normal, start the agitator 6 and reread the above steps until the monitored performance parameters are lower than the target value or enter the purification balance state.

[0079] After the purification is finished, based on the last reading of the integrated probe, a report is automatically generated by the controller 42. The generated report can be saved and sent out in the form of an email.

[0080] The above are only optional embodiments of the present disclosure and are not intended to limit the present disclosure. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure should be included in the protection scope of the present disclosure.

Claims

1. A purification device, It is characterized in that The purification device comprises a container (1) for accommodating a dialysis bag, a monitoring component (2), a liquid replacement component (3) and a control component (4); The container (1) is filled with liquid, the dialysis bag is immersed in the liquid and connected to the container; At least part of the monitoring component (2) is located in the container (1) and is used to monitor at least one performance parameter of the liquid in the container (1) in real time; The liquid replacement assembly (3) is connected to the container (1); The control component (4) is electrically connected to the monitoring component (2) and the liquid replacement component (3) respectively, and is used to control the liquid replacement component (3) to replace the liquid in the container (1) according to the at least one performance parameter.

2. The purification device according to claim 1, It is characterized in that The monitoring component (2) comprises at least one of the following sensors: a conductivity sensor, a total dissolved solids sensor, a temperature sensor, a pH value sensor, and an ammonium ion count sensor.

3. The purification device according to claim 2, It is characterized in that The monitoring component (2) comprises at least two sensors, and the at least two sensors are integrated into an integrated probe.

4. The purification device according to claim 3, It is characterized in that The container (1) has a liquid inlet and a liquid outlet; The liquid replacement assembly (3) comprises a first driving pump (31) and a second driving pump (32), wherein the outlet of the first driving pump (31) is connected to the liquid inlet, and the inlet of the first driving pump (31) is connected to the new liquid device; The inlet of the second driving pump (32) is connected to the liquid outlet, the outlet of the second driving pump (32) is connected to the waste liquid device, and the first driving pump (31) and the second driving pump (32) are both connected to the control component (4).

5. The purification device according to claim 4, It is characterized in that The liquid replacement assembly (3) further comprises a first pipeline (33) and a second pipeline (34), wherein two ends of the first pipeline (33) are respectively connected to the outlet of the first drive pump (31) and the liquid inlet, and two ends of the second pipeline (34) are respectively connected to the inlet of the second drive pump (32) and the liquid outlet.

6. The purification device according to claim 4, It is characterized in that The control component (4) comprises a plurality of drive chips (41) and a controller (42); The driving chip (41) is connected to the sensor in a one-to-one correspondence; The plurality of driving chips (41) are all connected to the controller (42), and the controller (42) is respectively connected to the first driving pump (31) and the second driving pump (32).

7. The purification device according to claim 6, It is characterized in that The purification device further comprises a workbench (5), and the container (1) is connected to the workbench (5); The first driving pump (31), the second driving pump (32), and the driving chip (41) are all integrated in the workbench (5).

8. The purification device according to claim 7, It is characterized in that The workbench (5) comprises a power interface (51) and a USB interface (52), and the power interface (51) is electrically connected to the driving chip (41), the first driving pump (31), the second driving pump (32) and the integrated probe respectively; The USB interface (52) is connected to the driving chip (41) and is used to connect to the controller (42).

9. The purification device according to any one of claims 1 to 8, It is characterized in that The purification device further comprises a stirrer (6), wherein the stirrer (6) is located inside the container (1).

10. The purification device according to claim 9, It is characterized in that The purification device further comprises a workbench (5), wherein the workbench (5) has a placement plane (50), the bottom of the container (1) is located on the placement plane (50), and the placement plane (50) is a plane with variable magnetism when powered on; The stirrer (6) is a magnetic stirrer.

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