Microfluidic reaction derivatization device based on Peltier temperature control

Abstract

The utility model discloses a micro-fluidic reaction derivatization device based on Peltier temperature control, which comprises a Peltier refrigeration sheet, a heating micro-fluidic chip arranged on a heat release surface of the Peltier refrigeration sheet, and a cooling micro-fluidic chip arranged on a heat absorption surface of the Peltier refrigeration sheet, the communicating pipeline is connected with the heating micro-fluidic chip and the cooling micro-fluidic chip; wherein the top of the heating micro-fluidic chip is provided with two first inlets, the bottom of the heating micro-fluidic chip is provided with a first outlet, the bottom of the cooling micro-fluidic chip is provided with a second inlet, the top of the cooling micro-fluidic chip is provided with a second outlet, and the first inlet and the second outlet are communicated with each other. Meanwhile, the first outlet and the second inlet are communicated with the two ends of the communicating pipeline respectively; the device has the advantages that the heating and cooling advantages of the Peltier technology are fully exerted, and the mixing efficiency of the microfluidic technology is combined, so that more efficient and more accurate reaction control is realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of laboratory instruments and devices, and specifically relates to a microfluidic reaction derivatization device based on Peltier temperature control. Background Art

[0002] In HPLC detection, many substances need to be derivatized to improve their detectability, such as biological acids / bases, amines, antibiotics (polyether antibiotics are common) and amino acids. In post-column derivatization reactions, precise control of reaction temperature and sufficient mixing of reactants are very critical. The reaction temperature is often controlled by external heating (such as a water bath) or cooling (such as an ice bath), and an external pump system is required to mix the reactants and derivatization reagents. However, these traditional methods have some shortcomings, such as the inability to accurately control temperature, complex operating steps, long pipelines, slow diffusion, and uneven mixing.

[0003] Peltier technology is a technology that can achieve heating and cooling quickly and accurately. This technology can simultaneously meet the heating and cooling conditions required for derivatization. In addition, the Peltier module is very small, has no moving parts, and has a long service life. Microfluidics technology can quickly achieve full mixing of reactants by mixing reactants through microchannels without the need for long pipes. At present, no device combining Peltier technology and microfluidics has been found on the market. Summary of the invention

[0004] The utility model aims to provide an efficient, reliable and easy-to-operate microfluidic reaction derivatization device based on Peltier temperature control, which gives full play to the heating and cooling advantages of Peltier technology and combines the mixing efficiency of microfluidic technology to achieve more efficient and precise reaction control.

[0005] The technical scheme of the utility model is: a microfluidic reaction derivatization device based on Peltier temperature control, comprising a Peltier cooling plate, a heating microfluidic chip arranged on the heat release surface of the Peltier cooling plate, a cooling microfluidic chip arranged on the heat absorption surface of the Peltier cooling plate, and a connecting pipeline connecting the heating microfluidic chip and the cooling microfluidic chip; wherein two first inlets are arranged on the top of the heating microfluidic chip, a first outlet is arranged at the bottom of the heating microfluidic chip, a second inlet is arranged at the bottom of the cooling microfluidic chip, and a second outlet is arranged on the top of the cooling microfluidic chip, and the first outlet and the second inlet are respectively connected to the two ends of the connecting pipeline.

[0006] As a preferred technical solution, the heating microfluidic chip and the cooling microfluidic chip both include a shell, and a microfluidic channel arranged inside the shell and having a bow-shaped flow direction, wherein the microfluidic channel is composed of a plurality of central chambers arranged longitudinally and arranged laterally at equal intervals, and a plurality of branch pipes connecting adjacent central chambers.

[0007] As a preferred technical solution, the heating microfluidic chip and the cooling microfluidic chip are respectively attached to the heat release surface and the heat absorption surface of the Peltier cooling sheet through thermal conductive gel or thermal conductive silicone grease.

[0008] As a preferred technical solution, the housing includes a base for mounting the microfluidic channel, and a cover fixedly connected to the base by a plurality of screws.

[0009] As a preferred technical solution, the inner diameter of the microfluidic channel is 0.1-0.5 mm.

[0010] As a preferred technical solution, the heating microfluidic chip and the cooling microfluidic chip are selected from microfluidic chips made of glass, polyimide polymer, or silicon carbide.

[0011] The advantages of the utility model are:

[0012] 1. The microfluidic reaction derivatization device based on Peltier temperature control of the utility model fully utilizes the heating and cooling advantages of Peltier technology, and combines the mixing efficiency of microfluidic technology to achieve more efficient and precise reaction control. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. The drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] The utility model is further described below in conjunction with the accompanying drawings and embodiments:

[0015] Figure 1 It is a structural schematic diagram of the utility model;

[0016] Figure 2 This is a schematic diagram of the structure of the heating microfluidic chip of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the cooling microfluidic chip of the utility model;

[0018] Figure 4 This is a schematic diagram of the interior of the heating microfluidic chip of the utility model;

[0019] Figure 5 This is an internal schematic diagram of the cooling microfluidic chip of the utility model;

[0020] Among them: 1 Peltier cooling plate, 2 heating microfluidic chip, 3 cooling microfluidic chip, 4 connecting pipeline, 5 first inlet, 6 first outlet, 7 second inlet, 8 second outlet, 9 base, 10 cover, 11 central chamber, 12 branch pipelines, 13 screws. DETAILED DESCRIPTION

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with specific implementation methods.

[0022] First, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0023] Secondly, the present invention is described in detail using a structural schematic diagram, etc. In the detailed description of the present invention, for the convenience of explanation, the schematic diagram showing the structure of a Peltier temperature-controlled microfluidic reaction derivatization device will not be partially enlarged according to the general scale, and the schematic diagram is only an example, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional space of length, width and depth should be included.

[0024] Example: Refer to Figures 1 to 5 As shown, a microfluidic reaction derivatization device based on Peltier temperature control includes a Peltier cooling plate 1, a heating microfluidic chip 2 arranged on the heat release surface of the Peltier cooling plate 1, a cooling microfluidic chip 3 arranged on the heat absorption surface of the Peltier cooling plate 1, and a connecting pipe 4 connecting the heating microfluidic chip 2 and the cooling microfluidic chip 3; wherein two first inlets 5 are arranged on the top of the heating microfluidic chip 2, a first outlet 6 is arranged on the bottom of the heating microfluidic chip 2, a second inlet 7 is arranged on the bottom of the cooling microfluidic chip 3, and a second outlet 8 is arranged on the top of the cooling microfluidic chip 3, and the first outlet 6 and the second inlet 7 are respectively connected to the two ends of the connecting pipe 4.

[0025] In the present embodiment, the heating microfluidic chip 2 and the cooling microfluidic chip 3 both include a shell, and a microfluidic channel arranged inside the shell and having a bow-shaped flow direction, wherein the microfluidic channel is composed of a plurality of central chambers 11 arranged longitudinally and arranged laterally at equal intervals, and a plurality of branch pipes 12 connecting adjacent central chambers 11, wherein the inner diameter of the central chamber 11 is 0.5 mm, and the inner diameter of the branch pipe 12 is 0.2 mm.

[0026] In this embodiment, the heating microfluidic chip 2 and the cooling microfluidic chip 3 are respectively attached to the heat release surface and the heat absorption surface of the Peltier cooling sheet 1 through thermal conductive gel or thermal conductive silicone grease. By adjusting the power of the Peltier cooling sheet 1, the heating microfluidic chip 2 and the cooling microfluidic chip 3 reach the specified temperature (the temperature control range of the Peltier cooling sheet 1 is -55-150°C), wherein the heating temperature required for the heating microfluidic chip 2 is 85°C, and the cooling temperature required for the cooling microfluidic chip 3 is 50°C.

[0027] The housing in this embodiment includes a base 9 for mounting the microfluidic channel, and a cover 10 fixedly connected to the base 9 by a plurality of screws 13 .

[0028] In this embodiment, the heating microfluidic chip 2 and the cooling microfluidic chip 3 are selected from microfluidic chips made of glass, polyimide polymer, or silicon carbide.

[0029] The specific working process of the microfluidic reaction derivatization device of the present invention is as follows: the reaction liquid and the derivatization reagent enter the microfluidic channel in the heating microfluidic chip 2 from the two first inlets 5 respectively to be mixed, and are heated and mixed by the exothermic surface of the Peltier cooling plate 1, and then the mixed liquid enters the cooling microfluidic chip 3 through the first outlet 6 and the second inlet 7 in turn, and is cooled by the heat absorption surface of the Peltier cooling plate 1, thereby terminating the reaction, and finally the reacted mixed liquid flows out from the second outlet 8.

[0030] It should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A microfluidic reaction derivatization device based on Peltier temperature control, It is characterized in that It comprises a Peltier cooling plate, a heating microfluidic chip arranged on the heat release surface of the Peltier cooling plate, a cooling microfluidic chip arranged on the heat absorption surface of the Peltier cooling plate, and a connecting pipeline connecting the heating microfluidic chip and the cooling microfluidic chip; wherein two first inlets are arranged on the top of the heating microfluidic chip, a first outlet is arranged on the bottom of the heating microfluidic chip, a second inlet is arranged on the bottom of the cooling microfluidic chip, and a second outlet is arranged on the top of the cooling microfluidic chip, and the first outlet and the second inlet are respectively connected to the two ends of the connecting pipeline.

2. The microfluidic reaction derivatization device based on Peltier temperature control according to claim 1, It is characterized in that The heating microfluidic chip and the cooling microfluidic chip both include a shell, and a microfluidic channel arranged inside the shell and having a bow-shaped flow direction, wherein the microfluidic channel consists of a plurality of central chambers arranged longitudinally and arranged laterally at equal intervals, and a plurality of branch pipes connecting adjacent central chambers.

3. The microfluidic reaction derivatization device based on Peltier temperature control according to claim 1, It is characterized in that The heating microfluidic chip and the cooling microfluidic chip are respectively attached to the heat release surface and the heat absorption surface of the Peltier cooling sheet through heat conductive gel or heat conductive silicone grease.

4. The microfluidic reaction derivatization device based on Peltier temperature control according to claim 2, It is characterized in that The housing comprises a base for mounting the microfluidic channel, and a cover body fixedly connected to the base via a plurality of screws.

5. The microfluidic reaction derivatization device based on Peltier temperature control according to claim 2, It is characterized in that The inner diameter of the microfluidic channel is 0.1-0.5 mm.

6. The microfluidic reaction derivatization device based on Peltier temperature control according to claim 1, It is characterized in that The heating microfluidic chip and the cooling microfluidic chip are selected from microfluidic chips made of glass, polyimide polymer, or silicon carbide.

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