Cell oscillating and shaking device

Abstract

The utility model relates to the technical field of lentiviral vector preparation, in particular to a cell oscillating and shaking device which comprises a base, a box body is fixedly arranged on the upper side of the base, a supporting bottom plate is arranged in the box body, four corners of the supporting bottom plate are fixedly connected with the base through supporting assemblies, and the supporting bottom plate is provided with a carrying disc. Rubber rollers are arranged on the lower side of the supporting bottom plate at intervals and installed on the base through shaft seats, a driving motor is arranged on the left sides of the rubber rollers, multiple sets of first magnet strips are embedded in the surfaces of the rubber rollers, and second magnet strips are embedded in the supporting bottom plate. According to the magnetic oscillation principle, cells in the cell culture bottle can be uniformly oscillated, so that the uniformity and the consistency of cell treatment are improved, collision and physical damage possibly occurring in the manual shaking process are avoided, a milder and safer treatment environment is provided for the cells, the automation degree is high, and the operation is convenient. The physical burden of operators is greatly reduced, and the working efficiency and the accuracy are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of lentiviral vector preparation, in particular to a cell oscillating and shaking device. Background Art

[0002] Lentiviral vector refers to a viral vector derived from human immunodeficiency virus-1 (HIV-1). Lentiviral vector contains the genetic information required for packaging, transfection, and stable integration, and is the main component of the lentiviral vector system. With the assistance of lentiviral packaging plasmids and cell lines, lentiviral vectors carrying exogenous genes are packaged into infectious viral particles through viruses, and the exogenous genes are expressed in cells or living tissues by infecting cells or living tissues. Lentiviral vectors contain the genetic information required for packaging, transfection, and stable integration. Lentivirus packaging plasmids can provide all the auxiliary proteins required for transcription and packaging RNA into recombinant pseudovirus vectors. In order to produce high-titer viral particles, it is necessary to co-transfect cells with expression vectors and packaging plasmids at the same time, package the virus in the cells, and secrete the packaged pseudovirus particles into the extracellular culture medium. After centrifugation to obtain the supernatant, it can be directly used for host cell infection. After the target gene enters the host cell, it is reverse transcribed and integrated into the genome, thereby expressing the effector molecule at a high level.

[0003] Lentiviral vectors are key tools in gene therapy, cell therapy, and other biomedical research, and the uniform dispersion and oscillation of cells play a vital role in the preparation of lentiviral vectors. During the preparation of lentiviral vectors, operators usually manually shake the cell culture flask to ensure that the cells are evenly digested. However, the uniformity of this manual shaking method is difficult to guarantee, which may lead to inconsistent cell digestion effects, and bumps are prone to occur during the shaking process, which may cause physical damage to the cells. In addition, long-term shaking operations will greatly consume the operator's physical strength, thereby affecting the accuracy and efficiency of subsequent operations. Therefore, it is necessary to seek a cell oscillation and shaking device to replace the current manual shaking method, so as to improve the efficiency and safety of the upstream production process of lentivirus. Utility Model Content

[0004] In view of the existing deficiencies, the utility model provides a cell oscillating and shaking device, which solves the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A cell oscillation and shaking device comprises a base, a box body is fixedly provided on the upper side of the base, a supporting bottom plate is arranged inside the box body, four corners of the supporting bottom plate are fixedly connected to the base through supporting components, the supporting bottom plate is provided with a loading tray, rubber rollers are arranged at intervals on the lower side of the supporting bottom plate, the rubber rollers are installed on the base through shaft seats, a driving motor is arranged on the left side of the rubber roller, a driving pulley is equipped on the output end of the driving motor, a driven pulley is equipped on the front end of the rubber roller, the driven pulley is connected to the driving pulley through a transmission belt, a plurality of groups of first magnet bars are equidistantly embedded on the surface of the rubber roller, and a second magnet bar is embedded in the supporting bottom plate.

[0007] Furthermore, the position of the second magnet strip corresponds to the position of the rubber roller, and the second magnet strip has the same magnetic pole as the first magnet strip.

[0008] Furthermore, a box cover is provided on the upper side cover of the supporting bottom plate, and a sealing cover door which can be rotatably opened and closed is provided on the box cover.

[0009] Furthermore, the support assembly consists of a baffle, a support rod and a support spring. The support rod is arranged through the internal corner of the support, and the lower end of the support rod is fixedly connected to the base. The upper end of the support rod is provided with a baffle, and a support spring is sleeved on the annular surface of the support rod at the lower side of the support base plate.

[0010] Furthermore, a pressure plate is arranged on the upper side of the loading tray, screw rods are fixed on the left and right sides of the upper end surface of the loading tray, and the pressure plate is sleeved on the screw rods, a knob is screwed on the annular surface of the screw rod, and the knob is located on the upper side of the pressure plate.

[0011] Furthermore, rubber pads are provided at the four corners of the bottom of the base.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] 1. The utility model uses the principle of magnetic oscillation to ensure that the cells in the cell culture bottle can be evenly oscillated, thereby improving the uniformity and consistency of cell processing, avoiding bumps and physical damage that may occur during manual shaking, and providing a gentler and safer processing environment for cells. It also has a high degree of automation, greatly reducing the physical burden of operators and improving work efficiency and accuracy.

[0014] 2. In the utility model, the position of the second magnet bar corresponds to the position of the rubber roller, and the second magnet bar has the same magnetic pole as the first magnet bar. The magnetic poles of the two magnet bars are the same. When the rubber roller rotates, a repulsive magnetic force will be generated between the first magnet bar and the second magnet bar. Due to the repulsive magnetic force, the supporting bottom plate and the loading plate and the cell culture bottle thereon will be subjected to an up and down vibration force. This vibration will cause the cells in the cell culture bottle to be subjected to a uniform and gentle oscillation, thereby achieving oscillation treatment of the cells. This design uses the principle of magnetic oscillation to ensure that the cells in the cell culture bottle can be evenly subjected to the oscillation, thereby improving the uniformity and consistency of cell treatment, and at the same time can reduce the wear caused by direct contact of the mechanical structure.

[0015] 3. The upper side cover of the support bottom plate is provided with a box cover, and a rotatable sealed cover door is provided on the box cover. The existence of the box cover and the sealed cover door helps to maintain the specific environmental conditions required for cell culture, and can effectively isolate the external air and potential pollution sources by reducing the impact of the external environment on cell culture, and protect the cells from pollution. At the same time, the rotatable sealed cover door allows the operator to easily open and close the box cover, place and take out the cell culture bottle, and improves the convenience of operation.

[0016] 4. A pressure plate is provided on the upper side of the loading tray, and the pressure plate is used to directly press the cell culture bottle placed on the loading tray to ensure that the cell culture bottle can be firmly kept on the loading tray during the oscillation process and will not move or tip over due to the oscillation. Screws are fixed on the left and right sides of the upper end surface of the loading tray, and the pressure plate is sleeved on the screws. A knob is screwed on the annular surface of the screw, and the knob is located on the upper side of the pressure plate. The screw and the knob are convenient for the operator to adjust the height of the pressure plate as needed to adapt to cell culture bottles of different heights, and the pressure plate can be fixed. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0018] Figure 2 It is a schematic diagram of the structure of the utility model after the sealing cover door is opened.

[0019] Figure 3 It is a schematic diagram of the structure after the box cover, box body and sealing cover door are removed in the utility model.

[0020] Figure 4 It is a partial cross-sectional view of the utility model.

[0021] In the figure: 1. box cover; 2. box body; 3. base; 31. foot pad; 4. sealed cover door; 5. loading tray; 6. pressure plate; 61. screw rod; 62. knob; 7. support bottom plate; 8. support assembly; 81. baffle; 82. support rod; 83. support spring; 9. drive motor; 10. drive pulley; 11. transmission belt; 12. driven pulley; 13. rubber roller; 14. first magnet bar; 15. second magnet bar. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] Example:

[0024] like Figures 1 to 4 As shown, a cell oscillation and shaking device comprises a base 3, a box body 2 is fixedly arranged on the upper side of the base 3, a supporting bottom plate 7 is arranged inside the box body 2, four corners of the supporting bottom plate 7 are fixedly connected to the base 3 through a supporting assembly 8, a loading tray 5 is arranged on the supporting bottom plate 7, and rubber rollers 13 are arranged at intervals on the lower side of the supporting bottom plate 7, and the rubber rollers 13 are installed on the base 3 through an axle seat, a driving motor 9 is arranged on the left side of the rubber roller 13, a driving pulley 10 is arranged on the output end of the driving motor 9, a driven pulley 12 is arranged at the front end of the rubber roller 13, and the driven pulley 12 is connected to the driving pulley 10 through a transmission belt 11. A plurality of groups of first magnet strips 14 are equidistantly embedded on the surface of the rubber roller 13, and a second magnet strip 15 is embedded on the supporting bottom plate 7. This design solves the problem that in the prior art, during the preparation of lentiviral vectors, operators usually manually shake the cell culture bottle to ensure that the cells can be evenly digested. However, the uniformity of shaking in this manual shaking method is difficult to ensure, which may lead to inconsistent cell digestion effects, and bumps are prone to occur during the shaking process, which may cause physical damage to the cells. In addition, long-term shaking operations will greatly consume the operator's physical strength, thereby affecting the accuracy and efficiency of subsequent operations.

[0025] The position of the second magnet strip 15 corresponds to the position of the rubber roller 13. The second magnet strip 15 has the same magnetic pole as the first magnet strip 14. The magnetic poles of the two magnet strips are the same. When the rubber roller 13 rotates, a repulsive magnetic force will be generated between the first magnet strip 14 and the second magnet strip 15. Due to the repulsive magnetic force, the supporting bottom plate 7 and the loading plate 5 thereon and the cell culture bottle will be subjected to an up and down vibration force. This vibration will cause the cells in the cell culture bottle to be subjected to a uniform and gentle oscillation, thereby achieving an oscillation treatment of the cells. This design uses the principle of magnetic oscillation to ensure that the cells in the cell culture bottle can be uniformly subjected to the oscillation, thereby improving the uniformity and consistency of cell treatment, and at the same time can reduce the wear caused by direct contact of the mechanical structure.

[0026] The upper side cover of the support bottom plate 7 is provided with a box cover 1, and a rotatable sealing cover door 4 is provided on the box cover 1. The existence of the box cover 1 and the sealing cover door 4 helps to maintain the specific environmental conditions required for cell culture, and can effectively isolate the external air and potential pollution sources by reducing the impact of the external environment on cell culture, thereby protecting the cells from pollution. At the same time, the rotatable sealing cover door 4 allows the operator to easily open and close the box cover 1, place and take out the cell culture bottle, and improves the convenience of operation.

[0027] The support assembly 8 is composed of a baffle 81, a support rod 82 and a support spring 83. The support rod 82 is arranged at the inner corner of the support, and the lower end of the support rod 82 is fixedly connected to the base 3. The upper end of the support rod 82 is provided with a baffle 81, and the support spring 83 is sleeved on the annular surface of the support rod 82 at the lower side of the support bottom plate 7. The design can support the support bottom plate 7 through the support assembly 8, maintain the stability of the support bottom plate 7, and the support spring 83 can provide elasticity and buffering. When the support bottom plate 7 is subjected to the magnetic force from the rubber roller 13, the support spring 83 can absorb and disperse these forces, so that the support bottom plate 7 and the cell culture bottle on the loading plate 5 can oscillate smoothly and evenly.

[0028] A pressing plate 6 is provided on the upper side of the loading tray 5, and the pressing plate 6 is used to directly press the cell culture bottle placed on the loading tray 5, so as to ensure that the cell culture bottle can be firmly kept on the loading tray 5 during the oscillation process, and will not move or fall due to the oscillation. Screws 61 are fixed on the left and right sides of the upper end surface of the loading tray 5, and the pressing plate 6 is sleeved on the screw 61. A knob 62 is screwed on the annular surface of the screw 61, and the knob 62 is located on the upper side of the pressing plate 6. This design facilitates the operator to adjust the height of the pressing plate 6 as needed through the screw 61 and the knob 62 to adapt to cell culture bottles of different heights, and the pressing plate 6 can be fixed. By rotating the knob 62, the operator can easily adjust the position of the pressing plate 6. When the knob 62 is tightened, the pressing plate 6 will press the cell culture bottle downward; when the knob 62 is loosened, the pressing plate 6 will move upward to release the pressure on the cell culture bottle.

[0029] Rubber foot pads 31 are provided at the four corners of the bottom of the base 3. The foot pads 31 provide the device with stable support, which helps to maintain the overall balance of the device. Since the foot pads 31 are made of rubber material, they have a good friction coefficient and can increase the friction between the device and the placement surface, ensuring that the device remains stable during the oscillation operation and will not move or tip over due to vibration.

[0030] The working principle of the cell oscillation and shaking device is as follows: in actual use, the sealing cover door 4 is first opened, and the culture bottle containing cells is placed on the loading plate 5 and fixed by the pressing plate 6. The height of the pressing plate 6 can be adjusted by the screw 61 and the knob 62 to adapt to cell culture bottles of different heights. By adjusting the tightness of the knob 62, the degree of compression of the pressing plate 6 on the cell culture bottle can be controlled. Then the sealing cover door 4 is closed and the driving motor 9 is started. When the driving motor 9 is started, the driving pulley 10 is driven to rotate, and the power is transmitted to the driven pulley 12 at the front end of the rubber roller 13 through the transmission belt 11, thereby driving the rubber roller 13 to rotate. A plurality of groups of first magnet strips 14 are embedded on the surface of the rubber roller 13, and a second magnet strip 15 corresponding to the first magnet strip 14 is embedded on the supporting bottom plate 7. The magnetic poles of the two magnet strips are the same, so repulsive magnetic forces are generated between them. As the rubber roller 13 rotates, the repulsive magnetic forces between the first magnet strip 14 and the second magnet strip 15 will change periodically. The changing magnetic force acts on the supporting bottom plate 7 to make it vibrate up and down, thereby achieving an oscillating treatment of the culture bottle on the carrier plate 5 .

[0031] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is impossible to list all the implementation methods here. All obvious changes or modifications derived from the technical solution of the present invention are still within the protection scope of the present invention.

Claims

1. A cell oscillating and shaking device, comprising a base (3), characterized in that: A box body (2) is fixedly arranged on the upper side of the base (3), a support bottom plate (7) is arranged inside the box body (2), four corners of the support bottom plate (7) are fixedly connected to the base (3) through support components (8), a loading tray (5) is arranged on the support bottom plate (7), rubber rollers (13) are arranged at intervals on the lower side of the support bottom plate (7), the rubber rollers (13) are mounted on the base (3) through shaft seats, a driving motor (9) is arranged on the left side of the rubber roller (13), a driving pulley (10) is arranged on the output end of the driving motor (9), a driven pulley (12) is arranged on the front end of the rubber roller (13), the driven pulley (12) and the driving pulley (10) are connected to each other through a transmission belt (11), a plurality of groups of first magnet strips (14) are equidistantly embedded on the surface of the rubber roller (13), and a second magnet strip (15) is embedded in the support bottom plate (7).

2. The cell oscillating and shaking device according to claim 1, characterized in that: The position of the second magnet strip (15) corresponds to the position of the rubber roller (13), and the second magnet strip (15) and the first magnet strip (14) have the same magnetic pole.

3. The cell oscillating and shaking device according to claim 1, characterized in that: The upper side cover of the supporting bottom plate (7) is provided with a box cover (1), and the box cover (1) is provided with a sealing cover door (4) that can be rotatably opened and closed.

4. The cell oscillating and shaking device according to claim 1, characterized in that: The support assembly (8) is composed of a baffle (81), a support rod (82) and a support spring (83); the support rod (82) is arranged to penetrate the inner corner of the support, and the lower end of the support rod (82) is fixedly connected to the base (3); the upper end of the support rod (82) is provided with a baffle (81); and the support spring (83) is sleeved on the annular surface of the support rod (82) at a position below the support bottom plate (7).

5. The cell oscillating and shaking device according to claim 1, characterized in that: A pressure plate (6) is arranged on the upper side of the loading tray (5), screw rods (61) are fixedly arranged on the left and right sides of the upper end surface of the loading tray (5), and the pressure plate (6) is sleeved on the screw rod (61), and a knob (62) is screwed on the annular surface of the screw rod (61), and the knob (62) is located on the upper side of the pressure plate (6).

6. The cell oscillating and shaking device according to claim 1, characterized in that: Foot pads (31) made of rubber material are arranged at the four corners of the bottom of the base (3).

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