Laser confocal microscope

Abstract

The utility model discloses a laser confocal microscope, and belongs to the technical field of medical imaging devices. The device comprises a laser generating unit 1, a semi-transparent and semi-reflective element 2, a galvanometer unit 3, a lens group 4, a focusing lens 6 and a displacement detector 7, the utility model provides the moving unit to drive the pinhole detector to move so as to quickly acquire information of a plurality of images with different depths, through a pinhole moving mode, the shooting speed of depth images is improved, sample photos with different depths can be quickly shot, and the photos with different depths are not easy to misplace because the shooting speed is high, so that the shooting efficiency is improved. Especially for low-frequency moving objects, compared with an existing laser confocal microscope, the laser confocal microscope provided by the utility model has a better shooting effect on depth image acquisition.

Description

Technical Field

[0001] The utility model relates to a laser confocal microscope and belongs to the technical field of medical imaging devices. Background Art

[0002] The imaging method of laser confocal microscopy is different from that of traditional microscopes. It only images one point at a time, and uses pinholes to filter stray light to greatly improve its resolution. Then, the entire screen is imaged through a scanning device. This allows the laser confocal microscope to achieve high magnification while also having sufficient resolution.

[0003] Due to the existence of its pinhole, the laser confocal microscope can also image samples at different depths, so it can also construct a 3D profile of the sample by taking multiple depth images. However, the current laser confocal microscope achieves different depth image acquisition of the sample by moving the entire instrument or adjusting the focal length of the lens. The acquisition efficiency is relatively low, and the acquired depth photos are easily misplaced. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a laser confocal microscope, comprising: a laser generating unit 1, a semi-transmissive and semi-reflective element 2, a galvanometer unit 3, a lens group 4, a focusing lens 6, and a displacement detector 7;

[0005] The laser light emitted by the laser generating unit 1 is reflected by the semi-transparent and semi-reflective element 2 and then passes through the galvanometer unit 3 and the lens group 4 to be incident on the sample 5 to be tested. After being reflected by the sample 5 to be tested, the laser light passes through the lens group 4, the galvanometer unit 3 and the semi-transparent and semi-reflective element 2 and then is collected by the displacement detector 7.

[0006] The displacement detector 7 includes: a fixed platform, a sliding assembly, a motor and a pinhole detector, the fixed platform is driven by the sliding assembly to translate under the action of the motor, and the pinhole detector is installed on the fixed platform. In one embodiment, the laser generating unit 1 includes: a laser and a laser collimation unit.

[0007] In one embodiment, the semi-transmissive and semi-reflective element 2 is a semi-reflective and semi-mirror lens.

[0008] In one implementation, the galvanometer unit 3 includes: a galvanometer.

[0009] In one embodiment, the sliding assembly includes: a screw rod and a guide rail, and the fixed platform is fixedly connected to the screw rod.

[0010] In one embodiment, the guide rail is a double guide rail.

[0011] Advantages of the utility model:

[0012] The laser confocal microscope of the present invention provides a moving unit to drive the pinhole detector to move, so as to obtain image information of multiple images at different depths. By moving the pinhole, the speed of depth image shooting is improved, and sample photos at different depths can be quickly shot. Because of its fast shooting speed, photos at different depths are less likely to be misplaced, especially for objects moving at low frequencies. Compared with the existing laser confocal microscope, the present invention has a better shooting effect for depth image acquisition. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a light path diagram of the laser confocal microscope of the present utility model.

[0014] Figure 2 The imaging principle diagram of collecting images at different depths by moving the pinhole.

[0015] Figure 3 This is a schematic diagram of the structure of the displacement detector of the utility model.

[0016] Among them, 1 is the laser generating unit, 2 is the semi-transparent and semi-reflective element, 3 is the galvanometer unit, 4 is the lens group, 5 is the sample to be tested, 6 is the focusing lens, 7 is the displacement detector, 8 is the sample photos at different depths, 7-1 is the pinhole, and 7-2 is the detector. DETAILED DESCRIPTION

[0017] The following is a specific description of the utility model.

[0018] Example 1

[0019] like Figure 1 As shown, it is a light path diagram of the laser confocal microscope of the utility model, comprising: a laser generating unit 1, a semi-transmissive and semi-reflective element 2, a galvanometer unit 3, a lens group 4, a focusing lens 6, and a displacement detector 7;

[0020] The laser light emitted by the laser generating unit 1 is reflected by the semi-transparent and semi-reflective element 2 and then passes through the galvanometer unit 3 and the lens group 4 to be incident on the sample 5 to be tested. After being reflected by the sample 5 to be tested, the laser light passes through the lens group 4, the galvanometer unit 3 and the semi-transparent and semi-reflective element 2 and then is collected by the displacement detector 7.

[0021] The structure of the detector with a moving device is as follows Figure 3 As shown, it includes a motor, a lead screw, a fixed platform and a guide rail. The motor drives the lead screw to rotate to achieve the forward and backward translation of the fixed platform along the guide rail, so that the detector with a pinhole is fixed on the fixed platform to achieve displacement.

[0022] The working principle of this utility model:

[0023] By coordinating the galvanometer frequency and moving the pinhole detector 7, sample photos 8 at different depths can be obtained.

[0024] When the pinhole moves back and forth, the imaging principle at different positions is as follows: Figure 2 As shown, element 9 is equivalent to the combination of all elements between the sample 5 and the pinhole 7-1. When the pinhole is at 7-1-0, the sample image at the depth of 5-1-0 can be collected; similarly, when the pinhole is at 7-1-1, the sample image at the depth of 5-1-1 can be collected; when the pinhole is at 7-1-2, the sample image at the depth of 5-1-2 can be collected.

[0025] Therefore, the utility model can collect images of samples at different depths by driving the pinhole to move back and forth through the mobile unit. Compared with the existing solutions of adjusting the position of the entire instrument or adjusting the focal length of the lens, the operation of the utility model is simpler and more efficient. In addition, the shooting speed can be increased, so photos at different depths are less likely to be misplaced, especially for objects moving at a low frequency, and the shooting effect is better than the existing technology.

[0026] Although the present invention has been disclosed as above in terms of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with the technology may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A laser confocal microscope, characterized in that: include: Laser generating unit (1), semi-transmissive and semi-reflective element (2), galvanometer unit (3), lens group (4), focusing lens (6), displacement detector (7); The laser light emitted by the laser generating unit (1) is reflected by the semi-transmissive and semi-reflective element (2) and then sequentially passes through the galvanometer unit (3) and the lens group (4) to be incident on the sample to be tested (5). After being reflected by the sample to be tested (5), the laser light sequentially passes through the lens group (4), the galvanometer unit (3) and the semi-transmissive and semi-reflective element (2) to be collected by the displacement detector (7); The displacement detector (7) comprises: a fixed platform, a sliding component, a motor and a pinhole detector; the fixed platform is driven to translate by the sliding component under the action of the motor; and the pinhole detector is mounted on the fixed platform.

2. The laser confocal microscope according to claim 1, characterized in that: The laser generating unit (1) comprises: a laser and a laser collimating unit.

3. The laser confocal microscope according to claim 1, characterized in that: The semi-transmissive and semi-reflective element (2) is a semi-reflective and semi-mirror lens.

4. The laser confocal microscope according to claim 1, characterized in that: The galvanometer unit (3) comprises: a galvanometer.

5. The laser confocal microscope according to claim 1, characterized in that: The sliding assembly comprises a screw rod and a guide rail, and the fixed platform is fixedly connected to the screw rod.

6. The laser confocal microscope according to claim 1, characterized in that: The guide rails are double guide rails.

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