Culture device with multiple temperature gradients

By designing a multi-temperature gradient culture device in the light incubator, using metal shells, temperature control devices and cold light sources, the problem of small temperature control range of traditional light incubator is solved, and experimental conditions and stable sampling are achieved with multiple temperature values Effect.

CN222331899UActive Publication Date: 2025-01-10NINGBO UNIV
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Patent Information

Application Number
CN202421022949.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-13
Publication Date
2025-01-10
Estimated Expiration
2034-05-13

AI Technical Summary

Technical Problem

The temperature control range of traditional light incubators is relatively fixed, which cannot meet the needs of multi-temperature gradient experiments, and it is easy to affect the temperature of all samples during sampling.

Method used

A culture device including a metal shell, a rectangular array layout, a temperature control device and a cold light source are designed. The temperature control device realizes multi-temperature gradient control through parallel embedded heating templates and temperature monitoring modules, and is used to control the acrylic force. A cold light source is embedded on the board to provide stable light.

Benefits of technology

The expansion and stability of the temperature control range is achieved, allowing independent pickup and operation of the culture container, reducing the temperature influence during sampling, and enhancing the rigor and operability of the experiment.

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Abstract

The utility model discloses a culture device with multiple temperature gradients, which is applied to microalgae culture. The culture device comprises a metal shell, a plurality of groups of culture containers, a temperature control device, an acrylic plate and cold light sources, wherein the culture containers are positioned in the metal shell and are distributed in a rectangular array manner; the acrylic plate is fixedly mounted at the bottom of the metal shell; the cold light sources act on the corresponding culture containers. The temperature control device comprises a main machine fixedly installed at the position of the front surface of the metal shell, three sets of heating templates embedded in the metal shell in parallel, and a temperature monitoring module used for detecting the corresponding heating templates. The temperature control device is wide in temperature control range and more stable in temperature control, culture conditions with multiple temperature values can be established to guarantee rigorous related temperature experiments, meanwhile, single culture containers can be taken independently and do not influence one another, operability is high, and the temperature is kept stable when samples are taken.
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Description

Technical Field

[0001] The utility model relates to the technical field of microalgae cultivation, in particular to a cultivation device with multiple temperature gradients. Background Art

[0002] Laboratories usually use light incubators to cultivate microalgae. Although light incubators provide precise constant temperature and simulated light experimental conditions, and also have temperature and illumination program setting functions, the temperature control range of light incubators is relatively fixed, that is, only one temperature value can be set for the culture space, and the controllable range is small, which is not conducive to temperature-related experiments. At the same time, when taking samples from the currently used light incubators, operations such as opening and closing the door will have a certain impact on the temperature of all sample cultures. Utility Model Content

[0003] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a culture device with multiple temperature gradients to establish a culture mode with multiple temperature gradients.

[0004] A culture device with multiple temperature gradients, comprising:

[0005] Metal housing;

[0006] A plurality of culture containers arranged in a rectangular array, which are located in the metal shell;

[0007] A temperature control device, comprising three sets of heating templates embedded in parallel in the metal shell;

[0008] an acrylic plate fixedly mounted at the bottom of the metal housing; and

[0009] The cold light source acting on the corresponding culture container is embedded in the acrylic plate.

[0010] The above-mentioned culture device overcomes the problem that the temperature control range of the traditional light incubator is relatively fixed, that is, the culture space can only set one temperature value, the controllable range is small, and it is not conducive to conducting temperature-related experiments.

[0011] In one embodiment, the metal shell is a hollow rectangular parallelepiped structure, and the metal shell is made of aluminum or lead.

[0012] Furthermore, the metal shell is provided with a culture container placement groove which is adapted to the corresponding culture container.

[0013] Furthermore, the culture container is made of glass, and the culture container is inserted into the metal shell through a corresponding culture container placement groove.

[0014] In one of the embodiments, one group of the heating templates is located in the middle of the metal shell, and the remaining two groups of the heating templates are located at corresponding ends of the metal shell.

[0015] In one of the embodiments, the temperature control device also includes a host and a temperature monitoring module for detecting the corresponding heating template.

[0016] Furthermore, the host is fixedly installed at the front surface of the metal shell, the detection end of the temperature monitoring module extends into the side where the corresponding heating template is located, and the temperature monitoring module is connected to the host through a signal line.

[0017] In one embodiment, the acrylic plate is located below the culture container.

[0018] Furthermore, the acrylic plate is provided with a patch slot for installing a corresponding cold light source, and the cold light source adopts an LED patch lamp bead.

[0019] Compared with the prior art, the beneficial effects of the utility model are: the temperature control range is wide and the temperature control is more stable, and culture conditions with multiple temperature values ​​can be established to ensure the rigor of related temperature experiments. At the same time, the taking of a single culture container can be carried out independently without affecting each other, and the operability is strong, so that the temperature remains stable when taking samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Shown is a schematic structural diagram of a culture device with multiple temperature gradients provided by the utility model.

[0021] Figure 2 Shown Figure 1 Exploded diagram.

[0022] Main component symbols

[0023] 1. Metal shell; 2. Culture container; 3. Temperature control device; 4. Host; 5. Heating template; 6. Temperature monitoring module; 7. Acrylic plate; 8. Cold light source.

[0024] The above main component symbols are combined with the accompanying drawings and specific implementation methods to further illustrate the present invention in detail. DETAILED DESCRIPTION

[0025] The utility model is described in detail below in conjunction with the accompanying drawings.

[0026] See also Figure 1-2This embodiment provides a culture device with multiple temperature gradients, which is applied to microalgae culture. The culture device comprises a metal shell 1, a plurality of culture containers 2 arranged in a rectangular array in the metal shell 1, a temperature control device 3, an acrylic plate 7 fixedly mounted at the bottom of the metal shell 1, and a cold light source 8 acting on the corresponding culture container 2.

[0027] The metal shell 1 is a hollow rectangular parallelepiped structure, and the metal shell 1 is made of aluminum or lead. The metal shell 1 is provided with a culture container placement groove, which is compatible with the corresponding culture container 2. The culture container 2 is made of glass, and contains microalgae and its culture solution, and the culture container 2 is inserted into the metal shell 1 through the corresponding culture container placement groove. This embodiment adopts a structure in which multiple culture containers 2 are inserted into the corresponding culture container placement groove, so that the single culture container 2 can be taken independently and does not affect each other, overcoming the problem that when taking samples from the currently used light incubator, operations such as opening and closing the door will have a certain impact on the temperature of all sample cultures therein. It should be noted that in order to avoid wear caused by direct contact between the culture container 2 and the metal shell 1, the metal shell 1 is coated with a material with good heat transfer such as a rubber layer (not shown) and other shrinkage properties.

[0028] The temperature control device 3 includes a host 4 fixedly mounted on the front surface of the metal shell 1 , three groups of heating templates 5 embedded in the metal shell 1 in parallel, and a temperature monitoring module 6 for detecting the corresponding heating templates 5 .

[0029] The detection end of the temperature monitoring module 6 extends into the side where the corresponding heating template 5 is located, and the temperature monitoring module 6 is connected to the host 4 through a signal line. In this embodiment, the ambient temperature of the space inside the metal shell 1 is controlled and adjusted by a temperature control device to create suitable experimental conditions for cultivating microalgae. The temperature sensor used in the temperature monitoring module 6 of this embodiment has the following specific temperature control process: the host 4 is operated to set the operating temperature of the corresponding heating template 5, the heating template 5 is powered on and heated, and the effect of adjusting the ambient temperature in the device is achieved by combining the heat conduction effect of the metal shell 1, and the temperature monitoring module 6 is used to detect the ambient temperature of the corresponding heating template 5 for timely adjustment. It should be noted that the control device of this embodiment can also be equipped with a refrigeration component (not shown) to realize the cooling function, such as a refrigeration pipe or a cold cavity that provides cold energy through a compressor or other cold source, and the ambient temperature is cooled down by cooperating with the heat conduction effect of the metal shell 1, which will not be described in detail here.

[0030] One group of the heating templates 5 is located in the middle of the metal shell 1, and the remaining two groups of the heating templates 5 are respectively located at the corresponding two ends of the metal shell 1. In this embodiment, based on the design of arranging the heating templates 5 along the two ends and the middle, by setting the heating templates 5 at the two ends to different temperatures, a temperature difference is formed on the left and right sides of the device, and a gradient temperature difference is formed in conjunction with the metal shell 1 with good thermal conductivity, that is, the ambient temperature of each vertical row of culture containers 2 is the same, and there is a temperature difference between the environments of each horizontal row of culture containers 2, thereby establishing a multi-temperature gradient culture mode, overcoming the problem that the temperature control range of the traditional light incubator is relatively fixed, that is, the culture space can only set one temperature value, the controllable range is small, and it is not conducive to temperature-related experiments.

[0031] The cold light source 8 is embedded in the acrylic plate 7, and the acrylic plate 7 is located below the culture container 2. The acrylic plate 7 is provided with a patch slot for installing the corresponding cold light source 8, and the cold light source 8 uses an LED patch lamp bead. The cold light source 8 of this embodiment is described by taking a 0.06W power, model 0201 patch LED as an example. In order to prevent the heat light source from affecting the temperature state of the microalgae in the culture space, this embodiment uses a cold light source 8 with good illumination and low temperature to provide light to the culture container 2 from bottom to top. The culture device of this embodiment has a more stable culture temperature and a wide temperature control range. The culture space and operability are large. The light source can also be controlled to make the cultured algae or microorganisms grow more stably and reduce the impact of environmental changes on the sample. It should be noted that based on the starting point of reducing the cost of the device and facilitating the installation and maintenance of the cold light source 8, this embodiment uses an acrylic plate 7 as the bottom plate of the metal shell 1. In actual use, in order to prevent light leakage, a circle of opaque film can be wrapped around the acrylic plate 7, which will not be repeated here.

[0032] Considering that during the microalgae cultivation process, when the microalgae settle and concentrate at the bottom of the culture container 2, it will affect the light, the culture device in this case is also equipped with a shaking structure (not shown), such as placing the culture device on an oscillator of model HY-3, so as to achieve the effect of mixing the microalgae.

[0033] In summary, the culture device of this embodiment has the following advantages compared to the light incubator currently used for culturing microalgae in laboratories: the culture device of this embodiment has a wide temperature control range and a more stable temperature control, and can establish culture conditions with multiple temperature values ​​to ensure the rigor of related temperature experiments. At the same time, the removal of a single culture container 2 can be carried out independently without affecting each other, and the operability is strong, so that the temperature remains stable when taking samples.

[0034] The naming of each component involved is based on the functions described in the specification, and is not limited to the specific nouns used in the utility model. Those skilled in the art may also choose other nouns to describe the names of the components of the utility model.

Claims

1. A culture device with multiple temperature gradients, characterized in that: include: Metal housing (1); A plurality of groups of culture containers (2) arranged in a rectangular array, which are located in the metal shell (1); A temperature control device (3), comprising three groups of heating templates (5) embedded in parallel in the metal shell (1); an acrylic plate (7) fixedly mounted at the bottom of the metal housing (1); and A cold light source (8) acting on the corresponding culture container (2) is embedded in the acrylic plate (7).

2. A culture device with multiple temperature gradients according to claim 1, characterized in that: The metal shell (1) is in the form of a hollow rectangular parallelepiped structure, and is made of aluminum or lead.

3. A culture device with multiple temperature gradients according to claim 2, characterized in that: The metal shell (1) is provided with a culture container placement groove extending therethrough, which is compatible with the corresponding culture container (2).

4. A culture device with multiple temperature gradients according to claim 3, characterized in that: The culture container (2) is made of glass, and the culture container (2) is inserted into the metal shell (1) via a corresponding culture container placement groove.

5. The culture device with multiple temperature gradients according to claim 1, characterized in that: One group of the heating templates (5) is located in the middle of the metal shell (1), and the remaining two groups of the heating templates (5) are located at corresponding ends of the metal shell (1).

6. The culture device with multiple temperature gradients according to claim 1, characterized in that: The temperature control device (3) also includes a host (4) and a temperature monitoring module (6) for detecting a temperature of a corresponding heating template (5).

7. A culture device with multiple temperature gradients according to claim 6, characterized in that: The host (4) is fixedly mounted on the front surface of the metal shell (1), the detection end of the temperature monitoring module (6) extends into the side corresponding to the heating template (5), and the temperature monitoring module (6) is connected to the host (4) via a signal line.

8. The culture device with multiple temperature gradients according to claim 1, characterized in that: The acrylic plate (7) is located below the culture container (2).

9. A culture device with multiple temperature gradients according to claim 8, characterized in that: The acrylic plate (7) is provided with a patch slot for installing a corresponding cold light source (8), and the cold light source (8) adopts an LED patch lamp bead.