Purification equipment and purification system comprising same

Abstract

The utility model provides purification equipment. The purification equipment comprises a magnetic rod assembly, a magnetic rod sleeve assembly, a placement assembly, a mounting frame, a first vertical moving mechanism, a second vertical moving mechanism and a horizontal moving mechanism, wherein the mounting frame is provided with a vertical sliding rail, and the vertical sliding rail is provided with a first sliding block used for fixing the magnetic rod frame and a second sliding block used for fixing the magnetic rod sleeve frame; the first vertical moving mechanism is used for driving the first sliding block to vertically move; the second vertical moving mechanism is used for driving the second sliding block to vertically move; and the horizontal moving mechanism is used for driving the mounting frame to move horizontally. The utility model further provides a purification system comprising the purification equipment. Compared with the existing equipment, the device disclosed by the utility model is simpler in structure, more reasonable in transmission layout, higher in driving reliability, smaller in volume ratio, beneficial to the intensive and miniaturized design of the whole extraction instrument, and convenient in later maintenance.

Description

Technical Field

[0001] The utility model relates to the field of purification equipment, in particular to a purification equipment and a purification system comprising the same. Background Art

[0002] With the development of molecular biotechnology and the improvement of scientific research and clinical testing requirements, nucleic acid purification methods have also experienced density gradient centrifugation, phenol chloroform, phenol, isoamyl alcohol extraction, silica adsorption and other chemical and physical methods. At the same time, protein purification has also experienced precipitation, gradient centrifugation, salting out, gel filtration chromatography, ion exchange chromatography, affinity chromatography, hydrophobic interaction chromatography and other chemical and physical methods. In terms of improving purification efficiency, magnetic particles have significant advantages. Magnetic particles are used in solid phase form in various purification processes. The target substance to be purified can adhere to the surface of the magnetic particles and move with the help of the magnetic field. The use of magnetic particles makes the available surface of the solid phase as large as possible.

[0003] Magnetic bead purification usually uses a purification system to separate magnetic beads through a magnetic field to achieve the purpose of separating and purifying cells, proteins, nucleic acids and other substances. Compared with the commonly used precipitation method, centrifugation method, and column membrane method, the magnetic bead purification method has the characteristics of high extraction efficiency, fast separation speed, and simple equipment required. Compared with resin purification, the magnetic bead purification method based on magnetic adsorption can quickly and effectively enrich the target biological molecules in the sample by relying on the combination of magnetic principle and incubation, effectively avoiding the drawbacks of resin on sample pretreatment and sample loading method, and getting rid of the limitations of column loading and flow rate. At present, the magnetic bead purification method based on magnetic particles not only meets the high quality requirements of scientific researchers for target biological molecules such as nucleic acids and proteins, but also its automation, high throughput, fast speed, and high sensitivity also improve the work efficiency of scientific researchers.

[0004] However, most of the current automatic magnetic bead purification equipment can realize the Z-axis upward linkage control of the connection or separation between the magnetic rod assembly and the magnetic rod sleeve assembly, while the X-axis or Y-axis movement on the horizontal plane is generally the linkage control of the porous reagent kit. Because the X-axis or Y-axis movement of the porous reagent kit on the horizontal plane is more for the installation of the porous reagent kit, the precision of the movement is not high, and the movement of the magnetic rod assembly and the magnetic rod sleeve assembly on the horizontal plane has further functions such as orderly switching between the slots of the reagent kit and stirring the reaction liquid, and the precision of the movement is higher. The existing movement control for the porous reagent kit cannot meet the above requirements. Although there are also devices in which the magnetic rod assembly and the magnetic rod sleeve assembly can also move in the X-axis, the structure is often more complicated to achieve the above functions, and the volume of the whole machine structure is very large. Therefore, there is an urgent need for a purification device or a purification system including a purification device to solve the above technical problems. Utility Model Content

[0005] In order to solve the above technical problems, the utility model provides a purification device, comprising: a magnetic rod assembly, a magnetic rod sleeve assembly and a placement assembly, a mounting frame, a first vertical moving mechanism and a second vertical moving mechanism and a horizontal moving mechanism;

[0006] The magnetic bar assembly comprises a magnetic bar rack for installing the magnetic bar;

[0007] The magnetic rod sleeve assembly comprises a magnetic rod sleeve frame for installing the magnetic rod sleeve, the magnetic rod sleeve frame is located below the magnetic rod frame, and each magnetic rod can enter the corresponding magnetic rod sleeve;

[0008] The placement component is used to place at least one multi-porous reagent box, and the placement component is located below the magnetic rod sleeve rack, and each of the magnetic rod sleeves can be correspondingly inserted into the corresponding multi-porous reagent box;

[0009] The mounting frame is equipped with a vertical slide rail, and the vertical slide rail is provided with a first slide block for fixing the magnetic rod frame and a second slide block for fixing the magnetic rod sleeve frame;

[0010] The first vertical movement mechanism is used to drive the first sliding block to move vertically;

[0011] The second vertical moving mechanism is used to drive the second sliding block to move vertically;

[0012] The horizontal movement mechanism is used to drive the mounting frame to move horizontally.

[0013] Specifically, it also includes the magnetic rod and / or the magnetic rod sleeve.

[0014] Specifically, the first vertical moving mechanism is a screw transmission structure. Further, the first vertical moving mechanism includes: a first motor, a first screw, and a first screw nut. The first motor is located at the bottom of the mounting frame. The output shaft of the first motor is vertically upward. The first screw is located on the output shaft of the first motor. The first screw nut is sleeved on the first screw, and the first screw nut is fixedly connected to the first slider.

[0015] Specifically, the second vertical moving mechanism is a screw transmission structure. Further, the second vertical moving mechanism includes: a second motor, a second screw, and a second screw nut. The second motor is located at the top of the mounting frame, and the output shaft of the second motor is vertically downward. The second screw is located on the output shaft of the second motor. The second screw nut is sleeved on the second screw, and the second screw nut is fixedly connected to the second slider.

[0016] Specifically, the vertical slide rail is located at the front side of the mounting frame, and the vertical slide rail is close to the magnetic bar assembly, the magnetic bar sleeve assembly and the placement assembly; and / or

[0017] The thrust range of the second motor is greater than that of the first motor; and / or the diameter of the second screw rod is greater than that of the first screw rod;

[0018] Preferably, the first screw rod is close to the vertical slide rail, and the second screw rod is arranged parallel to the first screw rod.

[0019] Specifically, the horizontal moving mechanism is a pulley transmission structure. Further, the horizontal moving mechanism includes: a synchronous pulley, an idler wheel, a belt, a third motor, and a horizontal slide rail. The belt connects the synchronous pulley and the idler wheel. The third motor is rotatably connected to the synchronous pulley. A pair of third sliders located at the bottom of the mounting frame are respectively arranged on the two single rails of the horizontal slide rail. The third slider is driven by the belt to drive the mounting frame to move horizontally. Further, the horizontal moving mechanism also includes a connecting block, one end of the connecting block is fixed to the belt, and the other end is fixedly connected to the mounting frame. Further, the connecting block and the mounting frame are integrally formed.

[0020] Specifically, it also includes a base, and a working room is arranged at the front end of the base, and the working room is used to arrange the magnetic rod assembly, the magnetic rod sleeve assembly and the placement assembly. The working room is composed of a top plate, a back plate and two left and right side plates. The working room has an opening facing the user. Furthermore, a vertical opening is provided in the middle of the back plate for the first slider and the second slider to partially extend into the working room.

[0021] Specifically, the rear end of the base is used to set the mounting frame and the horizontal moving mechanism, and further, the mounting frame and the horizontal moving mechanism are close to the back plate; the circuit structure integrated board and the power supply device of the purification equipment are installed behind the base, and further, the circuit structure integrated board is parallel to the back plate in the vertical direction.

[0022] Specifically, a partition is provided between the rear plate and the mounting frame, and the partition can move with the mounting frame. The partition can cover the opening in the middle of the rear plate without affecting the first slider and the second slider partially extending into the working chamber.

[0023] Specifically, it also includes a heat dissipation device for dissipating heat from the working room. Furthermore, the heat dissipation device is a fan. Furthermore, the heat dissipation device has one or more of the following characteristics:

[0024] (1) The fan is installed in any one of the following ways: embedded in the side panel, installed on the outside of the side panel, or installed on the inside of the side panel;

[0025] (2) There are two fans;

[0026] (3) The fan is mounted on the side panel and is located at the lower part of the side panel;

[0027] (4) The lower part of the side panel is provided with a heat dissipation hole, and the fan dissipates heat through the heat dissipation hole.

[0028] Specifically, it also includes a disinfection device for disinfecting the studio. Further, the disinfection device is an ultraviolet lamp. Further, an ultraviolet lamp is arranged in the top plate.

[0029] Specifically, the placement component includes a limiting device for fixing the multi-porous reagent kit.

[0030] Specifically, the placement component also includes a heating device for heating the porous test kit. Further, the heating device has a depression matching the bottom of the holes of the porous test kit, and the porous test kit is placed by matching the depression. Further, the heating device is a heating copper bar.

[0031] Specifically, it also includes an outer body structure, which includes: a frame, and an outer shell surrounding the frame. Furthermore, the frame is a rectangular frame, and a pair of first extension parts and second extension parts extending forward are symmetrically fixed on the left and right frames on the front side of the frame facing the user, respectively. The second extension part is longer than the first extension part, and the second extension part on the same frame is parallel to the first extension part and is located below the first extension part; the outer shell includes an installation front shell, and the installation front shell has a first opening parallel to the upper and lower parts for installing the display screen and a second opening for installing the cabin door, and the two sides of the installation front shell are fitted with the first extension part and the second extension part and are obliquely installed on the frame, and the rear upper side of the installation front shell is fitted with the front upper end side of the frame.

[0032] The utility model also provides a purification system, which comprises a purification device as described in any one of the above technical solutions and a controller for controlling the purification device to complete the purification operation.

[0033] Specifically, the controller includes any one or more of a cavity breaking module, a mixing module, a magnetic adsorption module, and a magnetic separation module:

[0034] The cavity breaking module controls the magnetic rod to be inserted into the magnetic rod sleeve, controls the magnetic rod and the magnetic rod sleeve to move downward to a specified position in the sleeve state, and vertically moves back and forth according to the set cavity breaking force and cavity breaking times to break the separation layer in the porous reagent box, so that the reaction liquids in the two reaction chambers separated by the separation layer are mixed;

[0035] The mixing module controls the magnetic rod sleeve to move vertically or horizontally according to the set stirring path, mixing speed, and mixing time to mix the reaction solution in the porous reagent box. Preferably, when the magnetic rod sleeve performs the mixing action, the magnetic rod and the magnetic rod sleeve are separated;

[0036] The magnetic adsorption module controls the magnetic rod and the magnetic rod sleeve to adsorb the target biological molecules bound to the magnetic beads in the sleeve state;

[0037] The magnetic separation module controls the magnetic rod to move upward and separate from the magnetic rod sleeve to obtain the eluate of the target biomolecule.

[0038] Compared with the prior art, the utility model has the following beneficial effects:

[0039] (1) The utility model uses only one vertical slide rail to realize the movement of the magnetic rod assembly and the magnetic rod sleeve assembly in the Z axis. The first slider and the second slider share one vertical slide rail, which not only saves one vertical slide rail, but also limits the movement distance of the magnetic rod assembly and the magnetic rod sleeve assembly in the Z axis, which is more conducive to the orderly switching of the magnetic rod assembly and the magnetic rod sleeve assembly between the slots of the multi-hole reagent box, and realizes the orderly transfer of magnetic beads. In addition, the entire Z-axis movement structure is installed on the mounting frame, and the mounting frame is driven by the horizontal movement mechanism as a whole, so that the overall horizontal movement can be realized, which further saves the installation structure;

[0040] (2) The mounting frame not only accommodates the first vertical moving mechanism and the second vertical moving mechanism, but also accommodates the vertical slide rail shared by the two moving mechanisms, and the structure is compact and easy to install;

[0041] (3) Both the first vertical moving mechanism and the second vertical moving mechanism use a screw nut to move up and down. Since the screw nut itself has a self-locking property, when the motor is powered off, the magnetic rod sleeve and the magnetic rod will not move freely downward due to their own gravity, which can effectively prevent the magnetic rod sleeve from colliding with the bottom of the porous reagent box, and can also effectively prevent the magnetic rod from colliding with the bottom of the magnetic rod sleeve;

[0042] (4) Compared with existing equipment, the structure is simpler, the transmission layout is more reasonable, the driving reliability is higher, and the volume is smaller, which is conducive to the overall intensive and miniaturized design of the extractor and convenient for later maintenance;

[0043] (5) In addition, by increasing the power of the output motor of the second vertical moving mechanism, the thrust range of the magnetic rod sleeve can be increased, which is more suitable for test kits that need to break the separation layer. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to more clearly illustrate the technical solution of the utility model, the following briefly introduces the drawings required for use in the utility model. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0045] Figure 1 It is a front view of the internal structure of the purification equipment provided by the utility model;

[0046] Figure 2 This is a rear view of the internal structure of the purification device provided by the utility model (without the circuit structure integrated board);

[0047] Figure 3 It is a magnetic rod assembly provided by the utility model;

[0048] Figure 4 The utility model provides a magnetic rod sleeve frame;

[0049] Figure 5 The utility model provides a magnetic rod sleeve;

[0050] Figure 6 It is the shell and frame (excluding the side shell) of the purification equipment provided by the utility model.

[0051] Among them, the reference numerals in the figure are: 1. shell, 11. front shell for installation, 2. frame, 21. first extension part, 22. second extension part, 3. base, 31. studio, 311. top plate, 312. back plate, 313. side plate, 32. mounting frame, 321. vertical slide rail, 322. first slider, 323. second slider, 33. partition, 34. fan, 4. magnetic rod assembly, 41. magnetic rod rack, 42. magnetic rod, 5. magnetic rod sleeve assembly, 51. magnetic rod sleeve rack, 52. magnetic rod sleeve, 6. placement Components, 61. Limiting device, 62. Heating copper strip, 63. Porous reagent kit, 7. Horizontal moving mechanism, 71. Third motor, 72. Synchronous pulley, 73. Idler pulley, 74. Belt, 75. Horizontal slide rail, 76. Connecting block, 77. Third slider, 8. First vertical moving mechanism, 81. First motor, 82. First screw rod, 83. First screw rod nut, 9. Second vertical moving mechanism, 91. Second motor, 92. Second screw rod, 93. Second screw rod nut, 10. Circuit structure integrated board. DETAILED DESCRIPTION

[0052] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0053] The "purification" of the present application is to extract or separate the target biomolecules by magnetic bead purification to obtain purified target biomolecules, and the purified target biomolecules include but are not limited to nucleic acids and proteins. Nucleic acid purification and protein purification have the same semantics as the common expressions "nucleic acid extraction" and "purified protein". In addition, the "purification" process may include the process of extracting from, for example, cell lysate and then purifying by magnetic bead adsorption, or it may be just the process of purifying by magnetic bead adsorption.

[0054] The utility model provides a purification device, comprising: a magnetic rod assembly 4, a magnetic rod sleeve assembly 5 and a placement assembly 6, a mounting frame 32, a first vertical moving mechanism 8 and a second vertical moving mechanism 9 and a horizontal moving mechanism 7; the magnetic rod assembly 4 comprises a magnetic rod frame 41 for mounting a magnetic rod 42; the magnetic rod sleeve assembly 5 comprises a magnetic rod sleeve frame 51 for mounting a magnetic rod sleeve 52, the magnetic rod sleeve frame 51 is located below the magnetic rod frame 41, and each magnetic rod 42 can enter the corresponding magnetic rod sleeve 52; the placement assembly 6 is used to place at least one porous reagent kit 63, The placement component 6 is located below the magnetic rod sleeve 51, and each magnetic rod sleeve 52 can be correspondingly inserted into the corresponding multi-hole reagent kit 63; the mounting frame 32 is equipped with a vertical slide rail 321, and the vertical slide rail 321 is provided with a first slider 322 for fixing the magnetic rod frame 41 and a second slider 323 for fixing the magnetic rod sleeve 51; the first vertical moving mechanism 8 is used to drive the first slider 322 to move vertically; the second vertical moving mechanism 9 is used to drive the second slider 323 to move vertically; the horizontal moving mechanism 7 is used to drive the mounting frame 32 to move horizontally. Specifically, as Figure 1-2 As shown, the present application uses only one vertical slide rail 321 to respectively realize the movement of the magnetic rod assembly 4 and the magnetic rod sleeve assembly 5 in the Z axis. The first slider 322 and the second slider 323 share one vertical slide rail 321, which not only saves one vertical slide rail 321, but also limits the movement distance of the magnetic rod assembly 4 and the magnetic rod sleeve assembly 5 in the Z axis, which is more conducive to the orderly switching of the magnetic rod assembly 4 and the magnetic rod sleeve assembly 5 between the slots of the porous reagent box 63, thereby realizing the orderly transfer of magnetic beads. In addition, the entire Z-axis moving structure is installed on the mounting frame 32. By driving the mounting frame 32 as a whole through the horizontal moving mechanism 7, the overall horizontal movement can be realized, further saving the mounting structure.

[0055] In one embodiment, the purification device further comprises a magnetic rod 42 and / or a magnetic rod sleeve 52. Specifically, Figure 3-5As shown, the magnetic rod 42 and the magnetic rod frame 41, and the magnetic rod sleeve 52 and the magnetic rod sleeve frame 51 are all detachable installation structures. The magnetic rod 42 and the magnetic rod sleeve 52 can be directly installed on the magnetic rod frame 41 and the magnetic rod sleeve frame 51, respectively, or installed when in use, so as to facilitate the disassembly, maintenance and replacement of the magnetic rod assembly 4 and the magnetic rod sleeve assembly 5. The utility model adopts the same magnetic bead purification principle as the prior art, that is, the magnetic field around the magnetic rod sleeve 52 is turned on and off by the up and down movement of the magnetic rod 42 relative to the magnetic rod sleeve 52. When the magnetic rod 42 is inside the magnetic rod sleeve 52, the magnetic beads are adsorbed to the surface of the magnetic rod sleeve 52, and follow the magnetic rod sleeve 52 to different slots of the porous reagent box 63; when the magnetic rod 42 is at the upper part of the magnetic rod sleeve 52, the magnetic beads are separated from the magnetic rod sleeve 52 and enter the reagent of the corresponding slot, and finally the purification of the target biological molecules is automatically realized.

[0056] In one embodiment, the first vertical moving mechanism 8 is a screw transmission structure. Further, the first vertical moving mechanism 8 includes: a first motor 81, a first screw 82, and a first screw nut 83. The first motor 81 is located at the bottom of the mounting frame 32. The output shaft of the first motor 81 is vertically upward. The first screw 82 is located on the output shaft of the first motor 81. The first screw nut 83 is sleeved on the first screw 82. The first screw nut 83 is fixedly connected to the first slider 322. Specifically, when the first motor 81 is powered on, the first screw 82 rotates and drives the first screw nut 83 to move along the first screw 82. At the same time, the first screw nut 83 links the first slider 322 and the magnetic rod assembly 4 to move up and down along the vertical slide rail 321. Correspondingly, the magnetic rod frame 41 and the magnetic rod 42 will also move up and down accordingly.

[0057] In the same embodiment, the second vertical moving mechanism 9 is a screw transmission structure. Further, the second vertical moving mechanism 9 includes: a second motor 91, a second screw 92, and a second screw nut 93. The second motor 91 is located at the top of the mounting frame, the output shaft of the second motor 91 is vertically downward, the second screw 92 is located on the output shaft of the second motor, the second screw nut 93 is sleeved on the second screw 92, and the second screw nut 93 is fixedly connected to the second slider 323. Specifically, when the second motor 91 is powered on, the second screw 92 rotates and drives the second screw nut 93 to move along the second screw 92. At the same time, the second screw nut 93 links the second slider 323 and the magnetic rod sleeve assembly 5 to move up and down along the vertical slide rail 321. Correspondingly, the magnetic rod sleeve frame 51 and the magnetic rod sleeve 52 will also move up and down accordingly.

[0058] The beneficial effect of adopting the above technical solution is that the first vertical moving mechanism 8 and the second vertical moving mechanism 9 both use a screw nut to move up and down. Since the screw nut itself has a self-locking property, when the motor is powered off, the magnetic rod sleeve 52 and the magnetic rod 42 will not move freely downward due to the action of their own gravity, which can effectively avoid the collision between the magnetic rod sleeve 52 and the bottom of the porous reagent kit 63, and can also effectively avoid the collision between the magnetic rod 42 and the bottom of the magnetic rod sleeve 52.

[0059] In one embodiment, the vertical slide rail 321 is located on the front side of the mounting frame, and the vertical slide rail 321 is close to the magnetic rod assembly 4, the magnetic rod sleeve assembly 5 and the placement assembly 6. The beneficial effect of adopting the above technical solution is: the vertical slide rail 321 is located on the front side of the mounting frame, and the first slider 322 and the second slider 323 are fixed on the vertical slide rail 321, which facilitates the first slider 322 and the second slider 323 to fix the magnetic rod frame 41 and the magnetic rod sleeve frame 51 on the front side of the mounting frame. Such a design structure is more compact.

[0060] In another embodiment, the thrust range of the second motor 91 is greater than that of the first motor 81; in other embodiments, the diameter of the second screw rod 92 is greater than that of the first screw rod 82; in other embodiments, the first screw rod 82 is close to the vertical slide rail 321, and the second screw rod 92 is arranged parallel to the first screw rod 82. The beneficial effect of adopting the above technical solution is that the magnetic rod sleeve 52 obtains a larger output thrust than the magnetic rod 42, which satisfies the function that the magnetic rod sleeve 52 has sufficient magnetic attraction force to adsorb magnetic beads after magnetization, and can also play the role of breaking the cavity, stirring, etc.

[0061] In one embodiment, the horizontal moving mechanism 7 is a pulley transmission structure. Further, the horizontal moving mechanism 7 includes: a synchronous pulley 72, an idler pulley 73, a belt 74, a third motor 71, and a horizontal slide rail 75. The belt 74 connects the synchronous pulley 72 and the idler pulley 73. The third motor 71 is rotatably connected to the synchronous pulley 72. A pair of third sliders 77 located at the bottom of the mounting frame 321 are respectively arranged on the two single rails of the horizontal slide rail 75. The third slider 77 is driven by the belt 74 to drive the mounting frame 32 to move horizontally. Further, the horizontal moving mechanism 7 also includes a connecting block 76. One end of the connecting block 76 is fixed on the belt 74, and the other end is fixedly connected to the mounting frame 32. Further, the connecting block 76 is integrally formed with the mounting frame 32. Specifically, when the third motor 71 is turned on, the synchronous pulley 72 rotates and drives the idler wheel 73 and the belt 74 to rotate together. At the same time, the belt 74 drives the connecting block 76 to move together, and the connecting block 76 is fixedly connected to the mounting frame 321 on the top of the slider 77, so the belt 74 drives the mounting frame 321 to move left and right along the horizontal slide rail 75, and accordingly, the magnetic bar 42 and the magnetic bar sleeve 52 arranged on the mounting frame 321 also move left and right accordingly.

[0062] In one embodiment, the purification device further comprises a base 3, a studio 31 is provided at the front end of the base 3, the studio 31 is used to set the magnetic rod assembly 4, the magnetic rod sleeve assembly 5 and the placement assembly 6, the studio 31 is composed of a top plate 311, a rear plate 312 and two left and right side plates 313, the studio 31 has an opening facing the user, and further, the middle of the rear plate 312 has a vertical opening for the first slider 322 and the second slider 323 to partially extend into the studio 31. Specifically, the utility model separates the experimental area from the mechanical control area through the studio 31, thereby avoiding the influence of the mechanical control area on the experiment.

[0063] In one embodiment, the rear end of the base 3 is used to set the mounting frame 32 and the horizontal moving mechanism 7, and further, the mounting frame 32 and the horizontal moving mechanism 7 are close to the rear plate 312; the circuit structure integrated board 10 and the power supply device of the purification equipment are installed behind the base 3, and further, the circuit structure integrated board 10 is parallel to the rear plate 312 in the vertical direction. Specifically, the base 3 is used to set the studio 31 at the front end, and the mounting frame 32 and the horizontal moving mechanism 7 are set at the rear end. The circuit structure integrated board 10 is set at the back of the base 3 so as to be separated from the circuit structure area, which further rationalizes the division of the structural area.

[0064] In one embodiment, a partition 33 is further provided between the rear plate 312 and the mounting frame 32, and the partition 33 can move with the mounting frame 32, and the partition 33 can cover the opening in the middle of the rear plate 312 without affecting the first slider 322 and the second slider 323 partially extending into the working room 31. Specifically, the partition 33 further strengthens the partition function of the working room 31, and at the same time, the partition 33 also ensures the overall appearance of the working room 31.

[0065] In one embodiment, the purification device further includes a heat dissipation device for dissipating heat from the working room 31. Furthermore, the heat dissipation device is a fan 34. Furthermore, the heat dissipation device has one or more of the following features: (1) the fan 34 is installed in any one of the following ways: embedded in the side plate 313, installed on the outside of the side plate 313, or installed on the inside of the side plate 313; (2) there are two fans 34; (3) the fan 34 is installed on the side plate and located at the bottom of the side plate; (4) there are heat dissipation holes at the bottom of the side plate 313, and the fan 34 dissipates heat through the heat dissipation holes. Specifically, the heat dissipation device shell ensures that the temperature in the working room 31 is in a suitable area, providing a stable temperature environment for the purification reaction.

[0066] In one embodiment, the purification device further comprises a disinfection device for disinfecting the working room 31. Furthermore, the disinfection device is an ultraviolet lamp. Furthermore, an ultraviolet lamp is arranged in the top plate. Specifically, the disinfection device is detachably fixed to a side of the top plate 311 facing the working room 31, and can perform ultraviolet disinfection on the working room 31.

[0067] In one embodiment, the placement assembly 6 includes a limiting device 61 for fixing the multi-porous reagent kit 63. Specifically, the limiting device 61 can fix the multi-porous reagent kit 63. If multiple multi-porous reagent kits 63 are required, there are multiple corresponding limiting devices 61.

[0068] In one embodiment, the placement component 6 further includes a heating device 62 for heating the porous reagent box 63. Further, the heating device has a depression matching the bottom of the hole of the porous reagent box 63, and the porous reagent box 63 is placed by matching the depression. Further, the heating device is a heating copper strip 62. Specifically, the heating device 62 can be easily disassembled and replaced, and maintenance is more convenient. The bottom of the porous reagent box 63 is kept warm by the heating device, thereby improving the efficiency of the purification process.

[0069] In one embodiment, the purification device also includes an external structure, which includes: a frame 2, and an outer shell 1 surrounding the frame. Furthermore, the frame 2 is a rectangular frame, and a pair of first extensions 21 and second extensions 22 extending forward are symmetrically fixed on the left and right frames on the front side of the frame 2 facing the user, respectively. The second extension 22 is longer than the first extension 21, and the second extension 22 on the same frame is parallel to the first extension 21 and is located below the first extension 21; the outer shell 1 includes an installation front shell 11, and the installation front shell 11 has a first opening 111 and a second opening 112 for installing a hatch, which are parallel to each other, respectively, for installing a display screen, and a second opening 112 for installing a cabin door. Both sides of the installation front shell 11 fit the first extension 21 and the second extension 22 and are obliquely installed on the frame 2, and the rear upper side of the installation front shell 11 fits the front upper end side of the frame 2. Specifically, the rear upper side of the installation front shell 11 refers to the upper side of the back of the installation front shell 11 facing away from the user, and the front upper end of the frame 2 refers to the uppermost frame on the front side of the frame 2. Figure 6 As shown, a pair of first extension parts 21 and second extension parts 22 of unequal lengths and parallel to each other are symmetrically fixed on the front side of the frame 2, and the two sides of the front shell 11 are fitted with the first extension parts 21 and the second extension parts 21 to be installed obliquely on the frame 2, and the upper rear side of the front shell 11 is fitted with the upper front end of the frame 2, and a first opening 111 for installing a display screen and a second opening 112 for installing a cabin door are provided in parallel above and below the front shell 11, so that the front shell 11 can be installed obliquely on the frame 2 as a whole. In this way, the structures of the front shell 11 and the frame 2 are simplified: the frame 2 is a rectangular frame, and can be mass-produced by simply setting the first extension part 21 and the second extension part 22. The entire rectangular panel of the front shell 11 can be tilted and fitted to the frame 2, and the display screen and the cabin door can be integrated into one mounting structure and tilted at the same time, which further simplifies the mounting structure.

[0070] The utility model also provides a purification system, which has a purification device as described in any one of the above technical solutions and a controller for controlling the purification device to complete the purification operation. Specifically, the purification system realizes automatic control of the purification operation through the controller.

[0071] In one embodiment, the controller includes any one or more of a cavity breaking module, a mixing module, a magnetic adsorption module, and a magnetic separation module:

[0072] The cavity breaking module controls the magnetic rod 42 to be inserted into the magnetic rod sleeve 52, controls the magnetic rod 42 and the magnetic rod sleeve 52 to move downward to a specified position in the sleeve state, and vertically moves back and forth according to the set cavity breaking force and cavity breaking times to break the partition layer in the porous reagent box 63, so that the reaction liquids in the two reaction chambers separated by the partition layer are mixed:

[0073] The mixing module controls the magnetic rod sleeve 52 to move vertically or horizontally according to the set stirring path, mixing speed, and mixing time to mix the reaction solution in the porous reagent box. Preferably, when the magnetic rod sleeve 52 is mixing, the magnetic rod 42 is separated from the magnetic rod sleeve; in addition, the stirring path refers to the route along which the magnetic rod sleeve 52 moves according to the position where it should be positioned during stirring.

[0074] The magnetic adsorption module controls the magnetic rod 42 and the magnetic rod sleeve 52 to adsorb the target biological molecules bound to the magnetic beads in the sleeve state;

[0075] The magnetic separation module controls the magnetic rod 42 to move upward and separate from the magnetic rod cover 52 to obtain an eluate of target biomolecules.

[0076] Specifically, the partition layer is made of a soft inert material that is not easy to react chemically with the chemical solution it contacts, so that it is easy to be broken open by subsequent operations, and under normal conditions, it is easy to be physically separated from water, such as by different specific gravity, such as floating on water. Therefore, a paraffin layer is preferred, because the chemical properties of paraffin are very stable and it is not easy to react chemically with the separated reagent buffer; paraffin is lighter than water, and after being broken, it automatically floats up without affecting the components in the solution; and it is soft and can be easily broken open to release the reaction reagents therein. When the partition layer is a paraffin layer, the paraffin fragments float directly on the liquid surface, which can not only allow the magnetic rod 42 and the magnetic rod sleeve 52 to pass through the separation position smoothly during the entire purification process and enter from one storage space to another storage space separated by the partition layer, but also avoid chemical or physical barriers to the purification process.

[0077] In one embodiment, taking the purification system for nucleic acid purification as an example, the operation steps are:

[0078] A. adding a sample into a suspension reaction chamber of a porous reagent kit for nucleic acid purification to obtain a sample suspension, wherein the porous reagent kit has a plurality of reaction chambers separated by a separation layer;

[0079] B. Under the control of the cavity breaking module, the magnetic rod 42 moves downward and is inserted into the magnetic rod sleeve 52, and moves downward to the set first cavity breaking position while maintaining the sleeve state, and moves back and forth vertically according to the set first cavity breaking force and the first cavity breaking number to break the first separation layer; under the control of the mixing module, the magnetic rod 42 moves upward alone and is separated from the magnetic rod sleeve 52, and the magnetic rod sleeve 52 moves vertically or horizontally according to the set stirring path, mixing speed, and mixing time to stir the sample suspension and lysate in the reaction chamber to obtain a first reaction solution in which the released plasmid DNA is dissolved;

[0080] C. Under the control of the cavity breaking module, the magnetic rod 42 moves downward for the second time and is inserted into the magnetic rod sleeve 52, and moves downward to the set second cavity breaking position while maintaining the sleeve state, and moves back and forth vertically multiple times according to the set second cavity breaking force and the second cavity breaking number to break the second separation layer; under the control of the mixing module, the magnetic rod 42 moves upward alone for the second time and is separated from the magnetic rod sleeve 52, and the magnetic rod sleeve 52 moves vertically or horizontally according to the set second stirring path, the second mixing speed, and the second mixing time to stir the first reaction liquid and the neutralization purification liquid solution in the reaction chamber to obtain the second reaction liquid dissolved with the revived and purified plasmid DNA;

[0081] D. Add magnetic beads to the release hole, and under the control of the magnetic adsorption module, the magnetic rod 42 moves downward for the third time to be inserted into the magnetic rod sleeve 52, and moves downward to the set magnetic attraction working position while maintaining the sleeve state, and maintains the sleeve magnetic attraction state according to the set magnetic attraction time and magnetic attraction speed;

[0082] E. Under the control of the magnetic separation module, the magnetic rod 42 and the magnetic rod cover 52 move upward in the sleeve state to the separation reagent kit, and move horizontally to the elution hole according to the set hole transfer path, and then move downward in the sleeve state to the set elution working position. The magnetic rod 42 moves upward alone for the third time, separates from the magnetic rod cover 52, and the magnetic beads fall into the elution solution. The magnetic rod cover 52 elutes at the set washing speed and set washing time to elute and separate the plasmid DNA from the magnetic beads.

[0083] In one embodiment, taking the purification system for protein purification as an example, the operation steps are:

[0084] A. The raw material magnetic bead suspension to be separated is contacted in the first hole group, and under the control of the mixing module, the magnetic rod sleeve 52 moves left or right or up and down according to the set stirring path, mixing speed, and mixing time to incubate the raw material magnetic bead suspension to be separated in the reaction chamber;

[0085] B. After the incubation reaction, under the control of the magnetic adsorption module, the magnetic rod 42 moves downward for the first time to be inserted into the magnetic rod sleeve 52, and moves downward to the set magnetic attraction working position in the first hole group while maintaining the sleeve state, and maintains the sleeve magnetic attraction state according to the set first magnetic attraction time and the first magnetic attraction speed; under the control of the magnetic separation module, the magnetic rod 42 and the magnetic rod sleeve 52 move upward for the first time in the sleeve state to be separated from the reagent kit, and move horizontally to the second hole group according to the set hole shifting path, and then move downward in the sleeve state to the set elution working position in the second hole group, the magnetic rod 42 moves upward alone for the first time to be separated from the magnetic rod sleeve 52, and the magnetic beads fall into the elution solution, and the magnetic rod sleeve 52 elutes according to the set first washing speed and the first washing time, so that the magnetic beads are fully eluted;

[0086] C. Under the control of the magnetic adsorption module, the magnetic rod 42 moves downward for the second time and is inserted into the magnetic rod sleeve 52, and moves downward to the set magnetic attraction working position in the second hole group while maintaining the sleeve state, and maintains the sleeve magnetic attraction state according to the set second magnetic attraction time and the second magnetic attraction speed; under the control of the magnetic separation module, the magnetic rod 42 and the magnetic rod sleeve 52 move upward for the second time in the sleeve state to be separated from the reagent kit, and are translated to the third hole group according to the set hole shifting path, and then move downward in the sleeve state to the set elution working position in the third hole group, the magnetic rod 42 moves upward alone for the second time, is separated from the magnetic rod sleeve 52, and the magnetic beads fall into the elution solution, and the magnetic rod sleeve 52 elutes according to the set second washing speed and the second washing time, so that the magnetic beads are fully eluted;

[0087] D. Under the control of the magnetic adsorption module, the magnetic rod 42 moves downward for the third time and is inserted into the magnetic rod sleeve 52, and moves downward to the set magnetic attraction working position in the third hole group while maintaining the sleeved state, and maintains the sleeved magnetic attraction state according to the set third magnetic attraction time and the third magnetic attraction speed; under the control of the magnetic separation module, the magnetic rod 42 moves upward alone for the third time, separates from the magnetic rod sleeve 52, and the magnetic beads fall into the eluent. The magnetic rod sleeve 52 elutes according to the set third washing speed and the third washing time, so that the magnetic beads are fully eluted to obtain the purified protein eluent.

[0088] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A purification device, characterized in that: include: A magnetic bar assembly, a magnetic bar sleeve assembly and a placement assembly, a mounting frame, a first vertical moving mechanism and a second vertical moving mechanism and a horizontal moving mechanism; The magnetic bar assembly comprises a magnetic bar rack for installing the magnetic bar; The magnetic rod sleeve assembly comprises a magnetic rod sleeve frame for installing the magnetic rod sleeve, the magnetic rod sleeve frame is located below the magnetic rod frame, and each magnetic rod can enter the corresponding magnetic rod sleeve; The placement component is used to place at least one multi-porous reagent box, and the placement component is located below the magnetic rod sleeve rack, and each of the magnetic rod sleeves can be correspondingly inserted into the corresponding multi-porous reagent box; The mounting frame is equipped with a vertical slide rail, and the vertical slide rail is provided with a first slide block for fixing the magnetic rod frame and a second slide block for fixing the magnetic rod sleeve frame; The first vertical movement mechanism is used to drive the first sliding block to move vertically; The second vertical moving mechanism is used to drive the second sliding block to move vertically; The horizontal movement mechanism is used to drive the mounting frame to move horizontally.

2. The purification device according to claim 1, characterized in that: Also included is the magnetic bar and / or the magnetic bar sleeve.

3. The purification device according to claim 1, characterized in that: The first vertical moving mechanism is a screw transmission structure. Further, the first vertical moving mechanism includes: a first motor, a first screw, and a first screw nut. The first motor is located at the bottom of the mounting frame. The output shaft of the first motor is vertically upward. The first screw is located on the output shaft of the first motor. The first screw nut is sleeved on the first screw, and the first screw nut is fixedly connected to the first slider.

4. The purification device according to claim 3, characterized in that: The second vertical moving mechanism is a screw transmission structure. Further, the second vertical moving mechanism includes: a second motor, a second screw, and a second screw nut. The second motor is located at the top of the mounting bracket, the output shaft of the second motor is vertically downward, the second screw rod is located on the output shaft of the second motor, the second screw rod nut is sleeved on the second screw rod, and the second screw rod nut is fixedly connected to the second slider.

5. The purification device according to claim 4, characterized in that: in, The vertical slide rail is located at the front side of the mounting frame, and the vertical slide rail is close to the magnetic bar assembly, the magnetic bar sleeve assembly and the placement assembly; and / or The thrust range of the second motor is greater than that of the first motor; and / or the diameter of the second screw rod is greater than that of the first screw rod.

6. The purification device according to claim 5, characterized in that: The first screw rod is close to the vertical slide rail, and the second screw rod is arranged parallel to the first screw rod.

7. The purification device according to claim 1, characterized in that: The horizontal moving mechanism is a pulley transmission structure. Further, the horizontal moving mechanism includes: a synchronous pulley, an idler wheel, a belt, a third motor, and a horizontal slide rail. The belt connects the synchronous pulley and the idler wheel. The third motor is rotatably connected to the synchronous pulley. A pair of third sliders located at the bottom of the mounting frame are respectively arranged on the two single rails of the horizontal slide rail. The third slider is driven by the belt to drive the mounting frame to move horizontally. Further, the horizontal moving mechanism also includes a connecting block, one end of the connecting block is fixed to the belt, and the other end is fixedly connected to the mounting frame. Further, the connecting block and the mounting frame are integrally formed.

8. The purification device according to claim 1, characterized in that: It also includes a base, and a working room is arranged at the front end of the base, and the working room is used to arrange the magnetic rod assembly, the magnetic rod sleeve assembly and the placement assembly. The working room is composed of a top plate, a back plate and two left and right side plates. The working room has an opening facing the user. Furthermore, a vertical opening is provided in the middle of the back plate for the first slider and the second slider to partially extend into the working room.

9. The purification device according to claim 8, characterized in that: The rear end of the base is used to set the mounting frame and the horizontal moving mechanism. Furthermore, the mounting frame and the horizontal moving mechanism are close to the back plate; the circuit structure integrated board and the power supply device of the purification equipment are installed behind the base. Furthermore, the circuit structure integrated board is parallel to the back plate in the vertical direction.

10. The purification device according to claim 8, characterized in that: A partition is also provided between the rear plate and the mounting frame. The partition can move with the mounting frame and can cover the opening in the middle of the rear plate without affecting the first slider and the second slider partially extending into the working chamber.

11. The purification device according to claim 8, characterized in that: It also includes a heat dissipation device for dissipating heat from the working room. Further, the heat dissipation device is a fan. Further, the heat dissipation device has one or more of the following characteristics: (1) The fan is installed in any one of the following ways: embedded in the side panel, installed on the outside of the side panel, or installed on the inside of the side panel; (2) There are two fans; (3) The fan is mounted on the side panel and is located at the lower part of the side panel; (4) The lower part of the side panel is provided with a heat dissipation hole, and the fan dissipates heat through the heat dissipation hole.

12. The purification device according to claim 8, characterized in that: It also includes a disinfection device for disinfecting the studio. Further, the disinfection device is an ultraviolet lamp. Further, an ultraviolet lamp is arranged in the top plate.

13. The purification device according to claim 1, characterized in that: The placement assembly includes a limiting device for fixing the multi-porous reagent kit.

14. The purification device according to claim 1, characterized in that: The placement component also includes a heating device for heating the porous reagent box. Further, the heating device has a depression matching the bottom of the holes of the porous reagent box, and the porous reagent box is placed by matching the depression. Further, the heating device is a heating copper bar.

15. The purification device according to claim 1, characterized in that: Also includes an outer body structure, the outer body structure includes: a frame, and a shell surrounding the frame, further, The frame is a rectangular frame, and a pair of first extension parts and second extension parts extending forward are symmetrically fixed on the left and right frames of the front side of the frame facing the user, the second extension part is longer than the first extension part, and the second extension part on the same frame is parallel to the first extension part and is located below the first extension part; The outer shell includes a mounting front shell, which has a first opening and a second opening parallel to each other for mounting a display screen and a hatch, and both sides of the mounting front shell are fitted in contact with the first extension portion and the second extension portion and are obliquely mounted on the frame, and the upper rear side of the mounting front shell is fitted in contact with the upper front end side of the frame.

16. A purification system, characterized in that: A purification device as described in any one of claims 1 to 15 and a controller for controlling the purification device to complete a purification operation.

17. The purification system according to claim 16, characterized in that: The controller includes any one or more of a cavity breaking module, a mixing module, a magnetic adsorption module, and a magnetic separation module: The cavity breaking module controls the magnetic rod to be inserted into the magnetic rod sleeve, controls the magnetic rod and the magnetic rod sleeve to move downward to a specified position in the sleeve state, and vertically moves back and forth according to the set cavity breaking force and cavity breaking times to break the separation layer in the porous reagent box, so that the reaction liquids in the two reaction chambers separated by the separation layer are mixed; The mixing module controls the magnetic rod sleeve to move vertically or horizontally according to the set stirring path, mixing speed, and mixing time to mix the reaction solution in the porous reagent box; The magnetic adsorption module controls the magnetic rod and the magnetic rod sleeve to adsorb the target biological molecules bound to the magnetic beads in the sleeve state; The magnetic separation module controls the magnetic rod to move upward and separate from the magnetic rod sleeve to obtain the eluate of the target biomolecule.

18. The purification system according to claim 17, characterized in that: When the magnetic rod sleeve performs a mixing action, the magnetic rod and the magnetic rod sleeve are separated.

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