Online liquid changing device for 96-well plate

Abstract

The utility model discloses an on-line liquid changing device for a 96-well plate. The on-line liquid changing device comprises a mounting table and an adjusting assembly, a 96-well plate is placed on the upper side of the mounting table, a storage assembly is mounted at the left end of the upper side of the mounting table, a liquid pumping assembly and a liquid injection assembly are mounted on the upper side of the mounting table, and the left ends of the liquid pumping assembly and the liquid injection assembly are both connected with the storage assembly; the adjusting assembly comprises electric telescopic rods, a movable frame, a fixed frame, a motor, a gear and a toothed plate, the two corresponding electric telescopic rods are mounted on the left side of the mounting table, the movable frame is fixed to telescopic arms of the two electric telescopic rods, an opening is formed in the upper side of the movable frame, the fixed frame is fixed into the opening, and the motor is mounted on the left side of the fixed frame; a gear is fixed to an output shaft of the motor, and two parallel toothed plates are arranged in the fixing frame. Liquid can be rapidly replaced, and the liquid replacing efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of pharmacokinetics, in particular to an online liquid replacement device for a 96-well plate. Background Art

[0002] 96-well plates are a common consumable in biological experiments, mainly used for PCR, fluorescent quantitative PCR and other experiments; 96-well plates have many and dense holes, the entire plate body is colorless and transparent, and there is no obvious marking between the holes. When plating cells on 96-well plates, the intermediate process requires multiple liquid changes. In drug screening, different drug concentration groups are involved, and different drug groups are also involved in in vitro efficacy. At present, when replacing the culture medium in cell experiments, an electric aspirator connected to a disposable 1ml gun head is used to suck the waste liquid from each hole one by one, and then add it one by one, which is relatively cumbersome. To this end, an online liquid exchange device for 96-well plates is proposed. Utility Model Content

[0003] The technical problem to be solved by the utility model is to overcome the existing defects and provide an online liquid changing device for a 96-well plate, which can quickly change the liquid, improve the liquid changing efficiency, and effectively solve the problems in the background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an online liquid replacement device for a 96-well plate, comprising a mounting platform and an adjustment component;

[0005] A 96-well plate is placed on the upper side of the mounting platform, a storage component is installed on the left end of the upper side of the mounting platform, a liquid extraction component and a liquid injection component are installed on the upper side of the mounting platform, and the left ends of the liquid extraction component and the liquid injection component are both connected to the storage component;

[0006] The adjusting component comprises an electric telescopic rod, a mobile frame, a fixed frame, a motor, a gear and a toothed plate. Two corresponding electric telescopic rods are installed on the left side of the mounting platform. The mobile frame is fixed on the telescopic arms of the two electric telescopic rods. An opening is provided on the upper side of the mobile frame. A fixed frame is fixed inside the opening. A motor is installed on the left side of the fixed frame. A gear is fixed on the output shaft of the motor. Two parallel toothed plates are arranged inside the fixed frame. The gear is meshed with the two toothed plates. A liquid changing component is installed inside the fixed frame. The input ends of the electric telescopic rod and the motor are electrically connected to the output end of the external control switch group. The positions of the liquid extraction tube and the liquid inlet tube are adjusted by setting the adjusting component.

[0007] Furthermore, the fluid exchange component includes a connecting frame, a connecting tube, a liquid extraction tube, a liquid exchange head and a liquid inlet tube, the interior of the fixed frame is slidably connected to two corresponding connecting frames, the tooth plate is fixed on the side of the connecting frame, a fixing hole is opened on the left side of the connecting frame, a connecting tube is fixed inside the fixing hole, and evenly distributed liquid extraction tubes are fixed on the circumferential surface of the connecting tube on the rear side, all liquid extraction tubes are communicated with the connecting tube on the rear side, and evenly distributed liquid inlet tubes are fixed on the circumferential surface of the connecting tube on the front side, all liquid inlet tubes are communicated with the connecting tube on the front side, all liquid inlet tubes are staggered with all liquid extraction tubes, and evenly distributed liquid exchange heads are installed at the lower ends of the circumferential surfaces of all liquid inlet tubes and all liquid extraction tubes, the liquid exchange heads located at the lower ends of the circumferential surfaces of all liquid extraction tubes correspond one-to-one to the holes on the 96-well plate, and liquid exchange is performed by setting up a fluid exchange component.

[0008] Furthermore, the liquid extraction component includes a liquid extraction hose, a liquid extraction pump and a return pipe. The liquid extraction hose is fixed to the left end inside the front connecting pipe, and the left end of the liquid extraction hose is fixed inside the water inlet of the liquid extraction pump. The liquid extraction pump is installed on the upper side of the mounting platform, and a return pipe is fixed inside the water outlet of the liquid extraction pump. The input end of the liquid extraction pump is electrically connected to the output end of the external control switch group, and the waste liquid is extracted by setting the liquid extraction component.

[0009] Furthermore, the injection assembly includes an injection hose, an injection pump and a fixed tube. The left end of the rear connecting tube is fixed with the injection hose, the left end of the injection hose is fixed inside the water outlet of the injection pump, the injection pump is installed on the upper side of the mounting table, the water inlet of the injection pump is fixed with a fixed tube, the input end of the injection pump is electrically connected to the output end of the external control switch group, and the new solution is injected into the interior of the 96-well plate by setting the injection assembly.

[0010] Furthermore, the storage assembly includes a storage box, a storage slot and a cover plate. The storage box is fixed on the left end of the upper side of the mounting platform. Two corresponding storage slots are arranged inside the storage box. The reflux pipe and the fixed pipe are fixed on the right side of the storage box. The reflux pipe and the fixed pipe are respectively communicated with the inner cavities of the two storage slots. The upper side of the storage box is clamped with a cover plate. By setting two storage slots, the waste liquid box medicine is stored separately.

[0011] Furthermore, two corresponding observation ports are provided on the front and rear sides of the storage box, and transparent plates are fixed inside the observation ports. The transparent plates enable the user to observe the situation inside the storage tank very conveniently.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] By setting the adjustment component, the motor can be started during use to rotate the gears and drive the two tooth plates to move in opposite directions. In this case, all the liquid changing heads fixed at the lower ends of the circumferential surfaces of all the liquid extraction tubes can be driven downward to be inserted into the holes on the inlet plate. The liquid extraction component can then be started to extract the waste liquid. After extraction, all the liquid changing heads are controlled to reset. After resetting, the two electric telescopic rods are controlled to contract so that all the liquid changing heads at the lower ends of the circumferential surfaces of all the liquid inlet tubes correspond one to one with the holes on the orifice plate. The liquid injection component can then be started to inject new liquid into the holes on the inlet plate. In this case, the liquid change can be completed without the need to change the liquid one by one, which greatly improves the efficiency of the liquid change. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the structure of the adjustment component of the utility model;

[0016] Figure 3 This is a schematic diagram of the structure of the liquid replacement component of the utility model;

[0017] Figure 4 This is a schematic diagram of the storage component structure of the utility model.

[0018] In the figure: 1 mounting table, 2 96-well plate, 3 adjustment component, 31 electric telescopic rod, 32 moving frame, 33 fixed frame, 34 motor, 35 gear, 36 tooth plate, 4 liquid exchange component, 41 connecting frame, 42 connecting pipe, 43 liquid extraction pipe, 44 liquid exchange head, 45 liquid inlet pipe, 5 liquid extraction component, 51 liquid extraction hose, 52 liquid extraction pump, 53 reflux pipe, 6 liquid injection component, 61 liquid injection hose, 62 liquid injection pump, 63 fixed pipe, 7 storage component, 71 storage box, 72 storage tank, 73 cover plate, 8 transparent plate. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] See also Figure 1-4 The present embodiment provides an online liquid exchange device for a 96-well plate, comprising a mounting platform 1 and an adjustment component 3; a 96-well plate 2 is placed on the upper side of the mounting platform 1, a storage component 7 is installed at the left end of the upper side of the mounting platform 1, and a liquid extraction component 5 and a liquid injection component 6 are installed on the upper side of the mounting platform 1.

[0021] Among them, the left ends of the liquid extraction component 5 and the liquid injection component 6 are both connected to the storage component 7. The liquid extraction component 5 includes a liquid extraction hose 51, a liquid extraction pump 52 and a return pipe 53. The left end of the front connecting pipe 42 is fixed with the liquid extraction hose 51. The left end of the liquid extraction hose 51 is fixed inside the water inlet of the liquid extraction pump 52. The liquid extraction pump 52 is installed on the upper side of the mounting platform 1. The return pipe 53 is fixed inside the water outlet of the liquid extraction pump 52. The input end of the liquid extraction pump 52 is electrically connected to the output end of the external control switch group.

[0022] Among them, the injection component 6 includes an injection hose 61, an injection pump 62 and a fixed pipe 63. The injection hose 61 is fixed to the left end of the rear connecting pipe 42, and the left end of the injection hose 61 is fixed to the inside of the water outlet of the injection pump 62. The injection pump 62 is installed on the upper side of the mounting platform 1, and the fixed pipe 63 is fixed to the inside of the water inlet of the injection pump 62. The input end of the injection pump 62 is electrically connected to the output end of the external control switch group.

[0023] Among them, the storage component 7 includes a storage box 71, a storage groove 72 and a cover plate 73. The storage box 71 is fixed at the left end of the upper side of the mounting table 1. Two corresponding storage grooves 72 are arranged inside the storage box 71. The reflux pipe 53 and the fixed pipe 63 are both fixed on the right side of the storage box 71. The reflux pipe 53 and the fixed pipe 63 are respectively communicated with the inner cavities of the two storage grooves 72. The upper side of the storage box 71 is clamped with a cover plate 73. By setting two storage grooves 72, the waste liquid box liquid is stored respectively, and the new liquid is injected into the interior of the 96-well plate 2 by setting the injection component 6, and the waste liquid is extracted by setting the extraction component 5.

[0024] Among them, the adjustment component 3 includes an electric telescopic rod 31, a mobile frame 32, a fixed frame 33, a motor 34, a gear 35 and a toothed plate 36. Two corresponding electric telescopic rods 31 are installed on the left side of the mounting platform 1. The mobile frames 32 are fixed on the telescopic arms of the two electric telescopic rods 31. An opening is opened on the upper side of the mobile frame 32, and a fixed frame 33 is fixed inside the opening. A motor 34 is installed on the left side of the fixed frame 33, and a gear 35 is fixed on the output shaft of the motor 34. Two parallel toothed plates 36 are arranged inside the fixed frame 33, and the gear 35 is meshed with the two toothed plates 36.

[0025] Among them, a liquid replacement component 4 is installed inside the fixed frame 33, the input ends of the electric telescopic rod 31 and the motor 34 are electrically connected to the output end of the external control switch group, the liquid replacement component 4 includes a connecting frame 41, a connecting tube 42, a liquid extraction tube 43, a liquid replacement head 44 and a liquid inlet tube 45, and the interior of the fixed frame 33 is slidably connected to two corresponding connecting frames 41, the tooth plate 36 is fixed on the side of the connecting frame 41, a fixing hole is opened on the left side of the connecting frame 41, and a connecting tube 42 is fixed inside the fixing hole, and evenly distributed liquid extraction tubes 43 are fixed on the circumferential surface of the connecting tube 42 on the rear side, and all the liquid extraction tubes 43 are The front connecting tube 42 is connected to the rear connecting tube 42, and is fixed with evenly distributed liquid inlet tubes 45 on its circumferential surface. All the liquid inlet tubes 45 are connected to the front connecting tube 42. All the liquid inlet tubes 45 are staggered with all the liquid extraction tubes 43. Evenly distributed liquid changing heads 44 are installed at the lower ends of the circumferential surfaces of all the liquid inlet tubes 45 and all the liquid extraction tubes 43. The liquid changing heads 44 located at the lower ends of the circumferential surfaces of all the liquid extraction tubes 43 correspond one by one to the holes on the 96-well plate 2. Liquid is changed by setting the liquid changing component 4, and the positions of the liquid extraction tubes 43 and the liquid inlet tubes 45 are adjusted by setting the adjustment component 3.

[0026] There are two corresponding observation ports on the front and rear sides of the storage box 71 , and a transparent plate 8 is fixed inside the observation ports. The transparent plate 8 allows the user to observe the situation inside the storage tank 72 very conveniently.

[0027] The working principle of the utility model is as follows:

[0028] First, the motor 34 is started to rotate the gear 35 to drive the two tooth plates 35 to move in opposite directions. The two tooth plates 35 move in opposite directions to drive the two connecting frames 41 to move in opposite directions. In this case, all the liquid extraction tubes 43 can be driven to move downward, and all the liquid inlet tubes 45 can be driven to move upward. At this time, all the liquid exchange heads 44 fixed at the lower ends of the circumferential surfaces of all the liquid extraction tubes 43 are inserted downward into the holes on the 96-well plate 2, and then the liquid extraction pump 52 is started to extract the waste liquid in the holes on the 96-well plate 2 into the interior of the storage tank 72 at the rear side;

[0029] Then the motor 34 is controlled to rotate, and the motor 34 rotates to make the gear 35 rotate to drive all the liquid changing heads 44 to reset. After resetting, the two electric telescopic rods 31 are controlled to contract to make all the liquid changing heads 44 at the lower ends of the circumferential surfaces of all the liquid inlet tubes 45 move. When all the liquid changing heads 44 at the lower ends of the circumferential surfaces of all the liquid inlet tubes 45 correspond to the holes on the 96-well plate 2 one by one, the injection pump 62 is started to inject the new liquid medicine inside the front storage tank 72 into the holes on the 96-well plate 2. In this case, the liquid change can be completed without changing the liquid one by one, which greatly improves the efficiency of the liquid change.

[0030] It is worth noting that the external control switch group disclosed in the above embodiments is provided with buttons corresponding to the electric telescopic rod 31, the motor 34, the liquid extraction pump 52 and the liquid injection pump 62. The electric telescopic rod 31 can use the TGC-A high-thrust electric telescopic rod, the motor 34 can use the 1LE0003 three-phase asynchronous motor, and the liquid extraction pump 52 and the liquid injection pump 62 can use the WQD6-12-0.55L1 water pump.

[0031] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An online liquid exchange device for a 96-well plate, characterized in that: It comprises a mounting platform (1) and an adjustment component (3); A 96-well plate (2) is placed on the upper side of the mounting platform (1), a storage assembly (7) is installed on the left end of the upper side of the mounting platform (1), a liquid extraction assembly (5) and a liquid injection assembly (6) are installed on the upper side of the mounting platform (1), and the left ends of the liquid extraction assembly (5) and the liquid injection assembly (6) are both connected to the storage assembly (7); The adjustment component (3) comprises an electric telescopic rod (31), a mobile frame (32), a fixed frame (33), a motor (34), a gear (35) and a toothed plate (36). Two corresponding electric telescopic rods (31) are installed on the left side of the mounting platform (1). The mobile frame (32) is fixed on the telescopic arms of the two electric telescopic rods (31). An opening is provided on the upper side of the mobile frame (32). A fixed frame (33) is fixed inside the opening. A motor (34) is installed on the left side of the fixed frame (33). A gear (35) is fixed on the output shaft of the motor (34). Two parallel toothed plates (36) are arranged inside the fixed frame (33). The gear (35) meshes with the two toothed plates (36). A liquid replacement component (4) is installed inside the fixed frame (33). The input ends of the electric telescopic rod (31) and the motor (34) are electrically connected to the output end of an external control switch group.

2. The online liquid replacement device for a 96-well plate according to claim 1, characterized in that: The liquid exchange assembly (4) comprises a connecting frame (41), a connecting pipe (42), a liquid extraction pipe (43), a liquid exchange head (44) and a liquid inlet pipe (45); two corresponding connecting frames (41) are slidably connected inside the fixing frame (33); the tooth plate (36) is fixed on the side of the connecting frame (41); a fixing hole is opened on the left side of the connecting frame (41); a connecting pipe (42) is fixed inside the fixing hole; liquid extraction pipes (43) are evenly distributed on the circumferential surface of the connecting pipe (42) at the rear side; all the liquid extraction pipes (43) are fixed to the connecting pipe (42) at the rear side; ) are all connected to the connecting tube (42) at the rear side, and evenly distributed liquid inlet tubes (45) are fixed on the circumferential surface of the connecting tube (42) at the front side, and all the liquid inlet tubes (45) are connected to the connecting tube (42) at the front side, and all the liquid inlet tubes (45) and all the liquid extraction tubes (43) are staggeredly distributed, and evenly distributed liquid replacement heads (44) are installed at the lower ends of the circumferential surfaces of all the liquid inlet tubes (45) and all the liquid extraction tubes (43), and the liquid replacement heads (44) located at the lower ends of the circumferential surfaces of all the liquid extraction tubes (43) correspond one to one to the holes on the 96-well plate (2).

3. The online liquid replacement device for a 96-well plate according to claim 2, characterized in that: The liquid extraction assembly (5) comprises a liquid extraction hose (51), a liquid extraction pump (52) and a return pipe (53); the liquid extraction hose (51) is fixed to the left end inside the front connecting pipe (42); the left end of the liquid extraction hose (51) is fixed inside the water inlet of the liquid extraction pump (52); the liquid extraction pump (52) is installed on the upper side of the mounting platform (1); the return pipe (53) is fixed inside the water outlet of the liquid extraction pump (52); the input end of the liquid extraction pump (52) is electrically connected to the output end of an external control switch group.

4. The online liquid replacement device for a 96-well plate according to claim 3, characterized in that: The liquid injection assembly (6) comprises a liquid injection hose (61), a liquid injection pump (62) and a fixed pipe (63); the liquid injection hose (61) is fixed to the left end inside the rear connecting pipe (42); the left end of the liquid injection hose (61) is fixed inside the water outlet of the liquid injection pump (62); the liquid injection pump (62) is installed on the upper side of the mounting platform (1); the fixed pipe (63) is fixed inside the water inlet of the liquid injection pump (62); the input end of the liquid injection pump (62) is electrically connected to the output end of an external control switch group.

5. The online liquid replacement device for a 96-well plate according to claim 4, characterized in that: The storage assembly (7) comprises a storage box (71), a storage slot (72) and a cover plate (73); the storage box (71) is fixed to the left end of the upper side of the mounting platform (1); two corresponding storage slots (72) are arranged inside the storage box (71); the return pipe (53) and the fixed pipe (63) are both fixed to the right side of the storage box (71); the return pipe (53) and the fixed pipe (63) are respectively communicated with the inner cavities of the two storage slots (72); and the cover plate (73) is clamped on the upper side of the storage box (71).

6. The on-line liquid replacement device for a 96-well plate according to claim 5, characterized in that: Two corresponding observation ports are provided on the front and rear sides of the storage box (71), and transparent plates (8) are fixed inside the observation ports.

Images