Sheet changing equipment and microscopic observation system comprising same

Abstract

The utility model provides a piece changing device and a microscopic observation system comprising the same, the piece changing device comprises a rotating disc, an observation platform and a transfer module, the rotating disc can rotate in a horizontal plane around the axis of the rotating disc, the upper surface of the rotating disc is provided with a plurality of first fixed clamping grooves, and the first fixed clamping grooves are circumferentially arranged by taking the axis as the center; the first fixed clamping groove is used for accommodating a chip; the observation platform is provided with a second fixing clamping groove penetrating through the observation platform in the vertical direction, and the position, relative to the rotary disc, of the observation platform is arranged in the mode that the second fixing clamping groove is located above one of the first fixing clamping grooves. The transfer module is arranged in the second fixed clamping groove, the transfer module can be switched between a first state and a second state, when the transfer module is in the first state, the transfer module moves the chip from the first fixed clamping groove below to the second fixed clamping groove, and when the transfer module is in the second state, the transfer module moves the chip from the first fixed clamping groove below to the second fixed clamping groove. And the chip falls back to the first fixed clamping groove below from the second fixed clamping groove.

Description

Technical Field

[0001] The utility model relates to the technical field of life science instruments, in particular to a film changing device and a microscopic observation system comprising the same. Background Art

[0002] The biochip contains microchambers or microdroplets that can be used for microbial culture, and its volume can be from 1 ml to 1 picoliter. The microchambers or microdroplets can contain microorganisms of different types or combinations, and can contain antibiotics of different types, concentrations and combinations. Microbial culture usually requires a specific gas environment and temperature, so the chip usually needs to be incubated for a certain period of time (e.g., 6 hours, 12 hours, 24 hours, etc.) at a specific gas component and a specific temperature. The incubation medium, environment, temperature and incubation time need to be selected according to different microorganisms.

[0003] By using microscopic imaging equipment such as bright field microscopes, fluorescence microscopes, and Raman imaging equipment, the growth state of microorganisms in the biochip can be observed and analyzed, and the impact of the microenvironment in which the microorganisms are located on the microorganisms can be further analyzed. For example, a microchamber or microdroplet containing a specific concentration of antibiotics can inhibit the growth of microorganisms therein. For another example, the cultured gas contains oxygen, which prevents anaerobic bacteria from growing in the microchamber or microdroplet. Therefore, microscopic imaging equipment combined with microfluidic chips can be used for precise microbial analysis or bacterial resistance detection.

[0004] When biochips are used for microbial analysis or bacterial resistance testing, it is usually necessary to scan the microchambers or microdroplets on the biochip, or to perform multiple imaging analyses on one of the microchambers or microdroplets. Therefore, the microscope platform usually needs to be combined with an electric displacement device to form an automatic observation platform to control the biochip to move the biochip in the xy or xyz direction, thereby completing the automated scanning of different positions on the biochip.

[0005] Since the existing automatic observation platform can usually only hold one biochip, the chip needs to be replaced manually, which is very cumbersome and prone to errors. Using an automated robotic arm for replacement requires high precision, the overall size of the device is large, and the price of the device is high. Utility Model Content

[0006] The technical problem to be solved by the utility model is to overcome the defect of easy error in manual chip replacement in the prior art, and to provide a chip replacement device and a microscopic observation system containing the same.

[0007] The utility model solves the above technical problems through the following technical solutions:

[0008] A film changing device, comprising:

[0009] A turntable, the turntable can rotate in a horizontal plane around the axis of the turntable, a plurality of first fixed card slots are arranged on the upper surface of the turntable, the plurality of first fixed card slots are arranged around the axis, and the first fixed card slots are used to accommodate chips;

[0010] An observation platform, wherein a second fixed slot is provided on the observation platform and passes through the observation platform in a vertical direction, and the position of the observation platform relative to the turntable is set so that the second fixed slot is located above one of the first fixed slots;

[0011] A transfer module, wherein the transfer module is arranged in the second fixed card slot, and the transfer module can switch between a first state and a second state. When the transfer module is in the first state, the transfer module moves the chip from the first fixed card slot below to the second fixed card slot. When the transfer module is in the second state, the chip falls back from the second fixed card slot to the first fixed card slot below.

[0012] A turntable is arranged in the film changing device, and a plurality of first fixed card slots are arranged in a ring shape around the axis of the turntable. The first fixed card slots are used to accommodate chips respectively, so that the openings of the first fixed card slots face upward so that the chips can leave the first fixed card slots upward. The observation platform above the turntable is provided with second fixed card slots corresponding to the first fixed card slots. The second fixed card slot is arranged directly above one of the first fixed card slots, so that the first fixed card slots arranged in a circle with the axis as the center can be located below the second fixed card slots in sequence through the rotation of the turntable, so as to realize the switching of the chip accommodated in each first fixed card slot and the position of the observation platform.

[0013] On this basis, a transfer module is provided in the second fixed card slot, and the chip is transferred between the corresponding first fixed card slot and the second fixed card slot by switching between the first state and the second state, so that the chip can be observed when the chip is located in the second fixed card slot of the observation platform. At the same time, the chip is dropped back to the first fixed card slot and the turntable is further rotated to achieve the purpose of replacing the chip.

[0014] Preferably, when the transfer module is in the first state, the transfer module is magnetic; when the transfer module is in the second state, the transfer module is not magnetic.

[0015] This technical solution provides a relatively more preferred solution for transferring chips by a transfer module, by making the transfer module magnetic in the first state, so as to adsorb the chip from the corresponding first fixed card slot to the second fixed card slot by magnetic adsorption. At the same time, by making the transfer module non-magnetic in the second state, the chip located in the second fixed card slot falls to the corresponding first fixed card slot under the action of gravity.

[0016] Preferably, the transfer module comprises a fixed block and a plurality of electromagnet sheets, the fixed block is provided with a first hollow portion penetrating the fixed block in a vertical direction, and the size of the first hollow portion is greater than or equal to the size of the observation area of ​​the chip;

[0017] The electromagnet sheets are dispersedly arranged on the surface of the fixed block, the electromagnet sheets can generate magnetism, and there are positioning sheets on the chip for the electromagnet sheets to adsorb. The number of the positioning sheets is equal to the number of the electromagnet sheets, and the positioning sheets are arranged one by one with the electromagnet sheets.

[0018] Magnetism is generated by setting multiple electromagnet sheets in the transfer module, and positioning sheets with the same number and corresponding positions as the electromagnet sheets are set at the same time. Each electromagnet sheet adsorbs the corresponding point chip, so that the transfer module can better position the chip through magnetic adsorption, avoiding the route deviation when the chip moves up. Fixed blocks are used to fix the multiple electromagnet sheets, so that the transfer module becomes an integral structure, which is more convenient to install and use relative to the observation platform.

[0019] Preferably, the size of the electromagnet sheet is equal to the size of the positioning sheet, and the maximum size of the electromagnet sheet is defined as a, where a≥2mm.

[0020] This technical solution provides a preferred size range for the electromagnet sheet and the positioning sheet. The suction force of the electromagnet sheet with a size greater than or equal to 2 mm on the positioning sheet is sufficient to suck up the chip.

[0021] Preferably, the size of the electromagnet sheet is equal to that of the positioning sheet, and the maximum size of the electromagnet sheet is defined as a, where a≤10 mm.

[0022] The technical solution provides an optimal size range for the electromagnet sheet and the positioning sheet. The electromagnet sheet with a size less than or equal to 10 mm ensures that it can be fully aligned when the positioning sheet is adsorbed. The first fixed card slot and the second fixed card slot can be made to just the size to accommodate the chip without too much margin.

[0023] Preferably, the positioning sheet is arranged on the upper surface of the chip.

[0024] By arranging the positioning sheet on the upper surface of the chip, the chip will not affect the magnetic field of the electromagnet sheet.

[0025] Preferably, the electromagnet sheet is arranged on the lower surface of the fixing block.

[0026] By arranging the electromagnet sheet on the lower surface of the fixing block, the fixing block will not affect the magnetic field of the electromagnet sheet.

[0027] Preferably, the number of the electromagnet pieces is defined as N, where N≥3.

[0028] Preferably, the number of the positioning plates is four, and the four positioning plates are evenly distributed on the chip.

[0029] Preferably, the number of the first fixed slots is fourteen.

[0030] Preferably, the turntable is circular.

[0031] Compared with a square or other polygonal turntable, a circular turntable is more convenient for setting the first fixed slots distributed in a ring shape, and will not leave too much space outside the first fixed slots, making the structure more compact.

[0032] Preferably, the second fixing slot is arranged on one side of the observation platform.

[0033] By setting the second fixed card slot on one side of the observation platform, the turntable and the observation platform do not need to overlap too much in the vertical direction, and a smaller observation platform can be adapted to a larger turntable, so that the turntable can be provided with more first fixed card slots to accommodate more chips to be observed, thereby improving the chip changing efficiency.

[0034] Preferably, a second hollow portion penetrating the bottom wall in a vertical direction is provided on the bottom wall of the first fixing slot, and the size of the second hollow portion is smaller than the area of ​​the chip.

[0035] By arranging the second hollow portion on the bottom wall of each first fixed slot, it is convenient to take out the chip. At the same time, the size area of ​​the second hollow portion is smaller than the area of ​​the chip to prevent the chip from falling downward from the second hollow portion.

[0036] A microscopic observation system comprises the above-mentioned film changing device and a microscope objective lens, wherein the microscope objective lens is arranged above the observation platform and is arranged vertically toward the second fixed slot.

[0037] By arranging a chip changing device capable of improving chip changing efficiency in a microscopic observation system, the efficiency is higher when the microscopic observation system is used to observe the chip to achieve the experimental purpose.

[0038] The positive progressive effect of the utility model is that by arranging a turntable in the wafer changing device, the turntable can rotate around its axis and has a plurality of first fixed card slots distributed along the circumference, and a second fixed card slot that can be aligned with one of the first fixed card slots is arranged on the observation platform, and by using a transfer module, the chip can be switched between the first fixed card slot and the second fixed card slot to realize the observation of the chip, and by rotating the turntable, different first fixed card slots and the second fixed card slots can be aligned to achieve the purpose of replacing the observed chip, thereby improving the wafer changing efficiency, and compared with manual chip replacement, the wafer changing arrangement is less prone to errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 The figure is a schematic diagram of the three-dimensional structure of a microscopic observation system according to a preferred embodiment of the utility model.

[0040] Figure 2 It is a side view schematic diagram of a microscopic observation system according to a preferred embodiment of the utility model.

[0041] Figure 3 This is a schematic diagram of the structure of a chip according to a preferred embodiment of the utility model.

[0042] Description of reference numerals:

[0043] Microscope Observation System 001

[0044] Film changing equipment 1

[0045] Turntable 11

[0046] First fixed slot 111

[0047] The second hollow portion 1111

[0048] Observation Platform 12

[0049] Second fixed slot 121

[0050] Transfer Module 13

[0051] Fixed block 131

[0052] The first hollow portion 1311

[0053] Microscope objective 2

[0054] Chip 3

[0055] Micro chamber 31

[0056] Positioning piece 4 DETAILED DESCRIPTION

[0057] A preferred embodiment is given below, and the present invention is described more clearly and completely in conjunction with the accompanying drawings.

[0058] like Figure 1-Figure 3 As shown, this embodiment provides a microscopic observation system 001, which includes a microscope objective lens 2 and a film changing device 1, and the microscope objective lens 2 is arranged above the film changing device 1. Specifically, the film changing device 1 includes a turntable 11, an observation platform 12 and a transfer module 13. The turntable 11 can rotate in a horizontal plane around the axis of the turntable 11 (not shown in the figure), and a plurality of first fixed card slots 111 are arranged on the upper surface of the turntable 11. The plurality of first fixed card slots 111 are arranged around the axis as the center of the circumference, and the first fixed card slots 111 are used to accommodate the chip 3; the observation platform 12 is provided with a second fixed card slot 121 that passes through the observation platform 12 in the vertical direction, and the position of the observation platform 12 relative to the turntable 11 is The second fixed card slot 121 is arranged so as to be located above one of the first fixed card slots 111; the transfer module 13 is arranged in the second fixed card slot 121, and the transfer module 13 can switch between a first state and a second state. When the transfer module 13 is in the first state, the transfer module 13 moves the chip 3 from the first fixed card slot 111 below to the second fixed card slot 121; when the transfer module 13 is in the second state, the chip 3 falls back from the second fixed card slot 121 to the first fixed card slot 111 below.

[0059] A turntable 11 is arranged in the film changing device 1, and a plurality of first fixed card slots 111 are arranged in a ring shape around the axis of the turntable 11. The first fixed card slots 111 are respectively used to accommodate the chips 3, so that the openings of the first fixed card slots 111 face upward so that the chips 3 can move upward out of the first fixed card slots 111. The observation platform 12 above the turntable 11 is provided with second fixed card slots 121 corresponding to the first fixed card slots 111. The second fixed card slots 121 are arranged just above one of the first fixed card slots 111, so that the first fixed card slots 111 arranged in a circle around the axis can be sequentially located below the second fixed card slots 121 through the rotation of the turntable 11, so as to realize the switching of the chip 3 accommodated in each first fixed card slot 111 and the observation platform 12.

[0060] On this basis, a transfer module 13 is provided in the second fixed card slot 121, and the chip 3 is transferred between the corresponding first fixed card slot 111 and the second fixed card slot 121 by switching between the first state and the second state, so as to observe the chip 3 when the chip 3 is located in the second fixed card slot 121 of the observation platform 12. At the same time, the chip 3 is dropped back to the first fixed card slot 111 and the turntable 11 is further rotated to achieve the purpose of replacing the chip 3.

[0061] In this embodiment, the turntable 11 can use any method in the prior art to achieve the rotation of the turntable 11 around its axis, such as setting a rotating shaft and a motor at the center of the turntable 11, or setting a driving mechanism on the side of the turntable 11 to drive the turntable 11 to rotate around its axis. The specific solution for achieving the rotation will not be repeated here.

[0062] Specifically, the transfer module 13 can drive the chip 3 to switch between the first fixed card slot 111 and the second fixed card slot 121, that is, the transfer module 13 can make the chip 3 move upward to the second fixed card slot 121, and the observation platform 12 can move with the chip 3. Since the second fixed card slot 121 passes through the observation platform 12 in the vertical direction, the observation platform 12 does not block the chip 3 in the vertical direction, so that the chip 3 can be observed from above by the microscopic imaging device. After the observation is completed, the observation platform 12 returns to its original position, the first fixed card slot 111 and the second fixed card slot 121 are realigned, and the transfer module 13 can switch the chip 3 from the second fixed card slot 121 back to the first fixed card slot 111. Since the turntable 11 can rotate around its axis and the first fixed slots 111 are arranged in a central ring around the turntable 11, any fixed slot can be rotated to be directly below the second fixed slot 121, so that the chip 3 in any first fixed slot 111 can be driven by the transfer module 13 to switch positions, thereby replacing the chip 3, avoiding the cumbersome process of manually replacing the chip 3 and making it less prone to errors.

[0063] Specifically, in this embodiment, a preferred transfer module 13 is provided to drive the chip 3 to switch positions between the second fixed card slot 121 and the corresponding first fixed card slot 111, which is specifically implemented by electromagnetic adsorption. Compared with other solutions, electromagnetic adsorption is a non-contact method with fewer moving parts and higher reliability. In order to allow the chip to be adsorbed by the transfer module 13, a positioning piece is provided on the chip, and the positioning piece can be magnetically adsorbed by the transfer module 13.

[0064] Specifically, in this embodiment, when the transfer module 13 is in the first state, the transfer module 13 has magnetism; when the transfer module 13 is in the second state, the transfer module 13 does not have magnetism. By making the transfer module 13 have magnetism in the first state, the chip 3 is adsorbed from the corresponding first fixed card slot 111 to the second fixed card slot 121 by magnetic adsorption. At the same time, by making the transfer module 13 not have magnetism in the second state, the chip 3 located in the second fixed card slot 121 falls to the corresponding first fixed card slot 111 under the action of gravity.

[0065] Of course, in other embodiments, when the chip 3 is transferred by magnetic adsorption, the transfer module 13 can also be set to have a magnetism sufficient to adsorb the chip 3 in the first state, and a magnetism that cannot keep the chip 3 adsorbed in the second fixed card slot 121 in the second state so that the chip 3 falls back. In more embodiments, the transfer module 13 can also realize the switching of the chip 3 between the first fixed card slot 111 and the second fixed card slot 121 in the way of the prior art, which will not be repeated here.

[0066] In addition, in other embodiments, other methods other than electromagnetic adsorption may be used to enable the transfer module 13 to move the chip 3 between the second fixed card slot 121 and the corresponding first fixed card slot 111, such as relying on a lifting manipulator to grab the chip 3. Among them, the electromagnetic adsorption solution of this embodiment is relatively simpler in structure and has higher operational reliability than other feasible solutions.

[0067] Specifically in this embodiment, Figure 1 and Figure 3 As shown, the transfer module 13 includes a fixed block 131 and four electromagnet sheets. The fixed block 131 is provided with a first hollow portion 1311 that penetrates the fixed block 131 in the vertical direction. The size of the first hollow portion 1311 is greater than or equal to the size of the area where the microchamber 31 to be observed in the chip 3 is located, that is, the size of the observation area; the electromagnet sheets are dispersedly arranged on the surface of the fixed block 131, and the electromagnet sheets can generate magnetism. The number of positioning sheets 4 is equal to the number of electromagnet sheets, and the positioning sheets 4 are arranged one-to-one with the electromagnet sheets. Magnetism is generated by arranging multiple electromagnet sheets in the transfer module 13, and positioning sheets 4 are arranged that are equal to the number of electromagnet sheets and correspond to each other in position. Each electromagnet sheet adsorbs the corresponding point chip 3, so that the transfer module 13 has a better positioning effect when magnetically adsorbing the chip 3, and avoids the chip 3 from being offset when the route is moved up. The fixed block 131 is used to fix multiple electromagnet sheets, so that the transfer module 13 becomes an integral structure, which is more convenient to install and use relative to the observation platform 12.

[0068] Of course, in other embodiments, the transfer module 13 may also be formed by a plurality of dispersedly arranged electromagnet sheets, which are respectively fixed in the second fixed slots 121. The same number of positioning sheets 4 are arranged on the chip 3 and correspond to the electromagnet sheets one by one, so that multi-point positioning can be achieved to ensure that the electromagnet sheets accurately adsorb the chip 3. At the same time, the number of electromagnet sheets may also be three, five, etc. As long as the number of electromagnet sheets is greater than or equal to three, a better adsorption effect can be achieved.

[0069] In this embodiment, the size of the electromagnet sheet is equal to the size of the positioning sheet 4. The electromagnet sheet and the positioning sheet 4 in this embodiment are both circular, and the distance between the electromagnet sheet and the chip 3 is a, a=2mm. By setting the maximum size of the electromagnet sheet and the positioning sheet 4 to 2mm, the suction force of the electromagnet sheet on the positioning sheet 4 under this size is sufficient to suck up the chip 3 and fix it in the second fixed card slot 121, and the force of the electromagnet sheet on the chip 3 under this size is vertical, and the chip 3 will not be skewed when being sucked up. Therefore, the first fixed card slot 111 and the second fixed card slot 121 can be made into a size that can just accommodate the chip 3, and there is no need to set a margin to accommodate the offset of the chip 3.

[0070] Of course, in other embodiments, the maximum size range of the electromagnet sheet and the positioning sheet 4 can be arbitrarily selected from 2 mm to 10 mm, that is, if the electromagnet sheet and the positioning sheet 4 are circular, their maximum size is the diameter, and if the electromagnet sheet and the positioning sheet 4 are square, their maximum size is the diameter of the smallest circumscribed circle of the square. It has been experimentally determined that the suction force of the electromagnet sheet with a maximum size within this range is sufficient to accurately suck up the chip 3 and saves raw materials.

[0071] In this embodiment, if Figure 3 As shown, four positioning pieces 4 are symmetrically arranged on the upper surface of the chip 3, and the electromagnet piece is arranged on the lower surface of the fixing block 131. By arranging the positioning piece 4 on the upper surface of the chip 3 and the electromagnet piece on the lower surface of the fixing block 131, the chip 3 and the fixing block 131 will not affect the magnetic field of the electromagnet piece.

[0072] In this embodiment, if Figure 1 As shown, the turntable 11 is circular, and the number of the first fixed slots 111 is fourteen, and the fourteen first fixed slots 111 are evenly arranged along the circumference of the turntable 11. Compared with a square or other polygonal turntable 11, a circular turntable 11 is more convenient for setting the first fixed slots 111 distributed in an annular shape, and does not leave too much space outside the first fixed slots 111, making the structure more compact.

[0073] Of course, in other embodiments, the turntable 11 may also be a square, a regular hexagon, etc. The closer the shape of the turntable 11 is to a circle, the more material and space are saved. At the same time, the number of the first fixed slots 111 may also be freely set according to actual conditions.

[0074] In this embodiment, the second fixed card slot 121 is arranged on one side of the observation platform 12. By arranging the second fixed card slot 121 on one side of the observation platform, the turntable 11 and the observation platform 12 do not need to overlap too much in the vertical direction, and a smaller observation platform 12 can also adapt to a larger turntable 11, so that the turntable 11 can be provided with more first fixed card slots 111 to accommodate more chips 3 to be observed, thereby improving the chip changing efficiency.

[0075] In this embodiment, if Figure 1 As shown, a second hollow portion 1111 is provided on the bottom wall of the first fixed card slot 111 and passes through the bottom wall in the vertical direction, and the size and area of ​​the second hollow portion 1111 is smaller than the area of ​​the chip 3. By providing the second hollow portion 1111 on the bottom wall of each first fixed card slot 111, it is convenient to take the chip 3, and at the same time, the size and area of ​​the second hollow portion 1111 is smaller than the area of ​​the chip 3 to prevent the chip 3 from falling downward from the second hollow portion 1111.

[0076] Although the specific implementations of the utility model are described above, those skilled in the art should understand that this is only an example, and the protection scope of the utility model is defined by the attached claims. Those skilled in the art can make various changes or modifications to these implementations without departing from the principle and essence of the utility model, but these changes and modifications fall within the protection scope of the utility model.

Claims

1. A film changing device, characterized in that: These include: A turntable, the turntable can rotate around the axis of the turntable in a horizontal plane, a plurality of first fixed card slots are arranged on the upper surface of the turntable, the plurality of first fixed card slots are arranged around the axis, and the first fixed card slots are used to accommodate chips; An observation platform, wherein a second fixed slot is provided on the observation platform and passes through the observation platform in a vertical direction, and the position of the observation platform relative to the turntable is set so that the second fixed slot is located above one of the first fixed slots; A transfer module, wherein the transfer module is arranged in the second fixed card slot, and the transfer module can switch between a first state and a second state. When the transfer module is in the first state, the transfer module moves the chip from the first fixed card slot below to the second fixed card slot. When the transfer module is in the second state, the chip falls back from the second fixed card slot to the first fixed card slot below.

2. The film changing device according to claim 1, characterized in that: When the transfer module is in the first state, the transfer module has magnetism; When the transfer module is in the second state, the transfer module is not magnetic.

3. The film changing device according to claim 2, characterized in that: The transfer module includes a fixed block and a plurality of electromagnet sheets, the fixed block is provided with a first hollow portion penetrating the fixed block in a vertical direction, and the size of the first hollow portion is greater than or equal to the size of the observation area of ​​the chip; The electromagnet sheets are dispersedly arranged on the surface of the fixed block, the electromagnet sheets can generate magnetism, and there are positioning sheets on the chip for the electromagnet sheets to adsorb. The number of the positioning sheets is equal to the number of the electromagnet sheets, and the positioning sheets are arranged one by one with the electromagnet sheets.

4. The film changing device according to claim 3, characterized in that: The size of the electromagnet sheet is equal to the size of the positioning sheet, and the maximum size of the electromagnet sheet is defined as a, a≥2mm; And / or, the size of the electromagnet sheet is equal to the size of the positioning sheet, and the maximum size of the electromagnet sheet is defined as a, a≤10 mm.

5. The film changing device according to claim 3, characterized in that: The positioning sheet is arranged on the upper surface of the chip; And / or, the electromagnet sheet is arranged on the lower surface of the fixing block.

6. The film changing device according to claim 3, characterized in that: The number of the electromagnet pieces is defined as N, where N≥3.

7. The film changing device according to claim 6, characterized in that: The number of the positioning plates is four, and the four positioning plates are evenly distributed on the chip; And / or, the number of the first fixed slots is fourteen.

8. The film changing device according to any one of claims 1 to 7, characterized in that: The turntable is circular; And / or, the second fixing slot is arranged on one side of the observation platform.

9. The film changing device according to any one of claims 1 to 7, characterized in that: A second hollow portion is provided on the bottom wall of the first fixing slot and passes through the bottom wall in a vertical direction. The size of the second hollow portion is smaller than the area of ​​the chip.

10. A microscopic observation system, characterized in that: It includes: The film changing device according to any one of claims 1 to 9; A microscope objective lens is disposed above the observation platform and is disposed vertically toward the second fixing slot.

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