Sheep in-vitro insemination device

By setting up the separating cavities between sperm and egg cells in the sheep in vitro insemination device and connecting through channels, ensuring that sperm needs to move upstream to bind egg cells, the problem of poor embryo quality caused by direct contact between goat sperm and egg in the prior art is solved, and the probability of fertilization and embryo quality are improved.

CN222889068UActive Publication Date: 2025-05-23ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202421303606.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-07
Publication Date
2025-05-23
Estimated Expiration
2034-06-07

AI Technical Summary

Technical Problem

In existing assisted reproductive culture devices for sheep, the sperm of sheep is in direct contact with the egg, resulting in an increase in the probability of embryo formation with weak vitality and poor quality, affecting the clinical pregnancy rate.

Method used

A sheep in vitro insemination device is designed. By setting a first volume cavity in the dish for placing the sperm of the sheep and the second volume cavity for placing the egg cells of the sheep, and connecting both through channels, ensuring that the sperm needs to move upward into the cavity where the egg cells are located, so that the higher active sperm is preferred to bind to the egg cells.

Benefits of technology

Through spatial separation and height difference design, the probability of sheep sperm binding to egg cells and fertilization quality are improved, thereby improving the clinical pregnancy rate.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model belongs to the technical field of breeding instruments, and particularly relates to a sheep in-vitro insemination device. The sheep sperm dish comprises a dish body, a first containing cavity used for containing sheep sperms is embedded in the bottom of the dish body, the peripheral side of the first containing cavity is communicated with a plurality of second containing cavities used for containing sheep egg cells, and the second containing cavities are higher than the first containing cavity. The space where the sperms of the sheep are located is separated from the space where the egg cells of the sheep are located, during fertilization, the sperms of the sheep with high activity can be preferentially combined with the egg cells of the sheep, and then the fertilization probability and the fertilization quality are improved.
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Description

Technical Field

[0001] The utility model belongs to the technical field of breeding equipment, and in particular relates to a sheep in vitro insemination device. Background Art

[0002] Assisted in vitro fertilization is to remove sperm or egg cells from the body, process or culture them into embryos, and then implant them into the recipient, overcoming the obstacles of traditional treatments. The most familiar one is in vitro fertilization. In fact, the simplest sperm washing combined with uterine in vitro fertilization is also a type of in vitro fertilization.

[0003] The existing assisted reproductive culture device for sheep adopts a fertilization method of adding processed sheep sperm of a certain concentration directly into a container containing sheep eggs. Therefore, all sheep sperm can contact sheep eggs, which greatly increases the probability of weak, poor quality, or even deformed sheep sperm combining with sheep eggs, resulting in uneven quality of the obtained embryos and affecting the clinical pregnancy rate. Utility Model Content

[0004] In order to overcome the defects in the prior art, the utility model provides a sheep in vitro insemination device, which can improve the probability and quality of fertilization.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A sheep in vitro insemination device comprises a dish body, a first cavity for placing sheep sperm is embedded in the bottom of the dish body, a plurality of second cavities for placing sheep eggs are connected to the periphery of the first cavity, and the height of the second cavity is higher than that of the first cavity.

[0007] Preferably, a first cavity and a plurality of second cavities surrounding the first cavity form a group of culture rooms, and the device includes a plurality of groups of culture rooms.

[0008] Preferably, a passage opening is provided at the connection between the first cavity and the second cavity, and the height of the passage opening is higher than the height of the bottom of the second cavity.

[0009] Preferably, the device further comprises a dish cover used in conjunction with the dish body.

[0010] Preferably, a fixing ring is sleeved on the outer wall of the dish body, a plurality of protrusions are arranged at intervals on the fixing ring, and a groove is arranged on the protrusion, and the groove is engaged with the dish cover.

[0011] Preferably, the height of the top of the second cavity is lower than the height of the mouth of the dish body.

[0012] Preferably, the inner bottom surface of the second cavity is provided with anti-slip grooves.

[0013] The advantages of the utility model are:

[0014] The utility model separates the space where the sheep's sperm and the sheep's egg cells are located by arranging the chamber and the compartment, and the second chamber where the sheep's egg cells are located is higher than the first chamber where the sheep's sperm are located, so that when the sperm and the egg cells combine, they need to swim upward to enter the second chamber, so that the sheep's sperm with higher activity can combine with the sheep's egg cells first, thereby improving the fertilization probability and fertilization quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of the utility model.

[0016] Figure 2 It is a schematic diagram of the front structure of the dish body of the utility model.

[0017] Figure 3 It is a schematic diagram of the top view of the dish body of the utility model.

[0018] The meanings of the symbols in the figure are as follows:

[0019] 1-dish body, 2-dish cover, 3-fixing ring, 4-bump, 5-groove, 6-first cavity, 7-second cavity, 8-channel opening. DETAILED DESCRIPTION

[0020] like Figure 1-3 As shown, a device for in vitro insemination of sheep is made of glass. The device is composed of a dish cover 2 and an open dish body 1. A fixing ring 3 is sleeved on the outer wall of the dish body 1. A plurality of protrusions 4 are arranged at intervals on the fixing ring 3. The protrusions 4 are provided with grooves 5. The grooves 5 are connected with the dish cover 2 by snapping to form an openable and closable structure.

[0021] Specifically, a first cavity 6 and a second cavity 7 are provided inside the dish body 1. The first cavity 6 is connected to a plurality of second cavities 7 for placing sheep's egg cells on its periphery. The first cavity 6 is used to place sheep's sperm. The second cavity 7 is connected to the first cavity 6 through a channel opening 8. The second cavity 7 is used to place sheep's egg cells.

[0022] Furthermore, a first cavity 6 for placing sheep sperm is embedded at the bottom of the dish body 1, and the height of the second cavity 7 is higher than that of the first cavity 6. By increasing the swimming distance of the sheep sperm, after the sheep sperm is reactivated, the high-quality sheep sperm will preferentially swim into the compartment and combine with the sheep egg cell, thereby increasing the probability of fertilization and ensuring the quality of the sheep embryo. The height of the channel opening 8 is higher than the height of the bottom of the second cavity 7, which can prevent the sheep egg cell from moving out of the channel opening 8 when the device is shaken or moved.

[0023] Furthermore, a first cavity 6 and a plurality of second cavities 7 surrounding the first cavity 6 form a group of culture rooms. The device includes a plurality of groups of culture rooms, which facilitates the use of one dish body 1 for multiple groups of experiments, or fertilization experiments of different types of sheep, thereby improving the utilization rate of the device.

[0024] The height of the top of the second cavity 7 is lower than the height of the mouth of the dish body 1 , ensuring that the culture fluid overflows the second cavity 7 , allowing sperm to swim smoothly into the second cavity 7 .

[0025] The inner bottom surface of the second cavity 7 is provided with anti-slip grooves, which can increase the friction at the bottom of the second cavity 7 and further prevent the sheep's egg cells from moving out of the second cavity 7.

[0026] In specific application, the dish body 1 is filled with culture fluid, and the sheep sperm to be reactivated is first placed in the first cavity 6, and the sheep egg cell is placed in the second cavity 7. One sheep egg cell is placed in one second cavity 7, and several sheep sperm are placed in the first cavity 6. After the sheep sperm is reactivated, the sheep sperm with higher quality will swim upward, preferentially enter the compartment through the channel opening and combine with the sheep egg cell, so as to form a high-quality sheep embryo.

[0027] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A sheep in vitro fertilization device, characterized in that: The device comprises a dish body (1), wherein a first cavity (6) for accommodating goat sperm is embedded at the bottom of the dish body (1), and a plurality of second cavities (7) for accommodating goat ova are connected to the periphery of the first cavity (6), wherein the height of the second cavity (7) is higher than that of the first cavity (6).

2. A sheep in vitro insemination device according to claim 1, characterized in that: One of the first containing chambers (6) and a plurality of second containing chambers (7) surrounding the first containing chamber (6) form a group of culture rooms, and the device comprises a plurality of groups of culture rooms.

3. A sheep in vitro insemination device according to claim 1, characterized in that: A passage opening (8) is provided at the connection between the first cavity (6) and the second cavity (7), and the height of the passage opening (8) is higher than the height of the bottom of the second cavity (7).

4. A sheep in vitro insemination device according to claim 1, characterized in that: The device also comprises a vessel cover (2) used in conjunction with the vessel body (1).

5. A sheep in vitro insemination device according to claim 4, characterized in that: A fixing ring (3) is sleeved on the outer wall of the dish body (1), a plurality of protrusions (4) are arranged at intervals on the fixing ring (3), a groove (5) is provided on the protrusion (4), and the groove (5) is snap-fitted and connected with the dish cover (2).

6. The in vitro insemination device for sheep according to claim 1, characterized in that: The height of the top of the second cavity (7) is lower than the height of the mouth of the dish body (1).

7. The in vitro insemination device for sheep according to claim 1, characterized in that: The inner bottom surface of the second cavity (7) is provided with anti-slip grooves.