Automatic chromatographic system with multiple columns connected in parallel

Abstract

The utility model discloses an automatic chromatographic system with multiple parallel columns, which is provided with more than two parallel chromatographic columns, and all the chromatographic columns share a mobile phase device, a detection device and other devices, and has the following advantages: (1) the parallel chromatographic columns share a set of software control system, so that the simultaneous operation of the multiple chromatographic columns is realized; the product analysis, separation and purification time is shortened, and the labor cost is reduced; (2) the chromatographic columns connected in parallel share a mobile phase device, a detector device, a fraction collector and other devices, and equipment is reduced; and (3) common parts are used, so that the plant area is reduced, the load-bearing requirement is reduced, and the load-bearing and area requirements of the plant are reduced, thereby reducing the construction cost.

Description

TECHNICAL FIELD

[0001] The utility model relates to a kind of chromatography systems, in particular to a kind of automatic chromatography systems of multiple column parallel connection. BACKGROUND

[0002] Chromatography system is the most effective method of current analysis, separation and purification of drugs, but limited to chromatography sample quantity, running time etc., capacity is limited, only used in high value-added products. The single cycle chromatography system commonly used in market at present, is using single column scheme, balance-sample-elution-column washing operation is circular, the chromatography system cannot realize automatic continuous operation, and only one chromatography column, purification efficiency is low, cannot meet the increasing demand of drug quality, and the need of production cost reduction. CN03146864.0 discloses another continuous chromatography system, using array chromatography column, solvent is equally sent into each column, using computer, feed control valve control, simulating annular chromatography feed mode sequentially, periodically, uniformly feeding chromatography column, eluting product is collected using special product collector, and is sent to each product storage container respectively, and sample is collected after detection. The chromatography column system uses single mobile phase, cannot purify multiple mobile phase samples, and chromatography column cannot be regenerated, reused, sample collection is not equipped with monitoring system, and there is risk. SUMMARY

[0003] Therefore, in order to overcome the deficiencies of the prior art, the utility model provides a kind of automatic chromatography systems of multiple column parallel connection, it is equipped with 2 or more parallel chromatography columns, all chromatography columns share mobile phase device and detection device and other devices, chromatography column is realized in a system Synchronous operation, greatly reduce the analysis, separation and purification time of product. Improve output efficiency, reduce manpower, equipment, plant cost at the same time.

[0004] To achieve the above object, the utility model provides a kind of scheme: a kind of automatic chromatography systems of multiple column parallel connection, including sample tank 1, mobile phase device 2, multiple-pass solvent selection valve 3, chromatography pump 4, chromatography column device 5, post-valve 6, detector device 7, fraction collector 8, waste liquid tank 9 and connecting pipeline;Mobile phase device includes at least 2 parallelly arranged mobile phase storage tanks, the number of chromatography pump is greater than or equal to 2, and is parallelly arranged, chromatography column device includes at least 2 identical and parallelly arranged chromatography columns, detector device includes at least 1 detector, and detector device is parallelly arranged with waste liquid tank;

[0005] The sample tank 1 and the mobile phase device 2 are connected with the multi-way solvent selection valve 3 through connecting pipes, the multi-way solvent selection valve 3 is connected with the chromatographic pump through connecting pipes, the chromatographic pump is connected with the chromatographic column device 5 through connecting pipes, the chromatographic column device 5 is connected with the post-column valve 6 through connecting pipes, the post-column valve 6 is connected with the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected with the fraction collector 8 through connecting pipes.

[0006] When the system is running, the liquid in the sample tank 1 and the mobile phase device 2 flows to the chromatographic pump through the multi-way solvent selection valve 3, the liquid treated by the chromatographic column is selected by the post-column valve 6 to flow to the detector device 7 or the waste liquid tank 9, and the liquid passing through the detector device 7 is collected by the fraction collector 8 connected with the detector device.

[0007] The utility model provides another scheme: a kind of multi-column parallel automatic chromatography system, including sample tank 1, mobile phase device 2, chromatographic pump 4, chromatographic column device 5, post-column valve 6, detector device 7, fraction collector 8, waste liquid tank 9, pre-column valve 10 and connecting pipe;

[0008] Its characterized in that, mobile phase device includes at least 2 parallelly arranged mobile phase storage tanks,

[0009] The number of chromatographic pump is greater than or equal to 3,

[0010] Chromatographic column device includes at least 2 same and parallelly arranged chromatographic columns,

[0011] Detector device includes at least 1 detector,

[0012] Detector device is parallelly arranged with waste liquid tank;

[0013] Among them, sample tank 1 is connected with chromatographic pump 4 through connecting pipe and forms sample delivery path, the parallelly arranged mobile phase storage tanks are connected with chromatographic pump 4 through connecting pipe respectively, and then are connected and communicated into mobile phase delivery path through connecting pipe, sample delivery path and mobile phase delivery path are connected with pre-column valve 10 through connecting pipe respectively, pre-column valve 10 is connected with chromatographic column device 5 through connecting pipe, chromatographic column device 5 is connected with post-column valve 6 through connecting pipe, post-column valve 6 is connected with parallel detector device 7 and waste liquid tank 9 through connecting pipe, and detector device 7 is connected with fraction collector 8 through connecting pipe.

[0014] When the system is running, the liquid in sample delivery path and mobile phase delivery path is selected to flow to chromatographic column device 5 by pre-column valve 10;The liquid treated by the chromatographic column is selected by post-column valve 6 to flow to detector device 7 or waste liquid tank 9, and the liquid passing through detector device (7) is collected by fraction collector 8 connected with detector device.

[0015] Preferably, the first scheme further comprises a pre-column valve 10, which is arranged before the chromatographic pump or the chromatographic column, and the liquid flows through the pre-column valve to the corresponding chromatographic pump or directly to the corresponding chromatographic column.

[0016] Preferably, in the two schemes, when the detector device comprises two or more detectors, the detectors are the same and are arranged in parallel; when the elution collection time is long, multiple detectors can be started at the same time to save the working time of the system and software.

[0017] Preferably, the chromatographic column in the two schemes is selected from a normal phase chromatographic column, a reversed phase chromatographic column, an ion exchange column, and a gel chromatographic column.

[0018] Preferably, the detector in the two schemes is selected from an ultraviolet detector, a conductivity detector, a diode array detector, an evaporative light scattering detector, and a mass spectrometry detector.

[0019] Preferably, in the two schemes, in the gradient mode, the chromatographic pump is selected from one or both of a common pump and a high-precision pump, which reduces the cost under the premise of meeting the elution requirements and ensuring the stability of the peak time.

[0020] Preferably, in the first scheme, in the gradient mode, the automatic chromatographic system further comprises a proportional valve 11, and the gradient elution mode can be controlled by the proportional valve 11.

[0021] Preferably, in the second scheme, in the gradient mode, the gradient elution mode can be controlled by the chromatographic pump 4.

[0022] Compared with the prior art, the above technical scheme has the following advantages:

[0023] ①The parallel chromatographic columns share a set of software control systems, realize the simultaneous operation of multiple chromatographic columns, reduce the analysis, separation and purification time of products, and reduce the labor cost;

[0024] ②The parallel chromatographic columns share the mobile phase device, the detector device, the fraction collector and other devices, and reduce the equipment;

[0025] ③The use of shared components reduces the factory area, reduces the bearing requirement, and reduces the factory bearing and area requirements, thereby reducing the construction cost. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 It is a structure diagram of the automatic chromatographic column system without a pre-column valve in the double-column isocratic mode described in embodiment 1.

[0027] Wherein: 1, sample tank, 2-1, the first mobile phase storage tank, 2-2, the second mobile phase storage tank, 2-3, the third mobile phase storage tank, 3-1, the first multi-pass solvent selection valve, 3-2, the second multi-pass solvent selection valve, 4-1, the first chromatographic pump, 4-2, the second chromatographic pump, 5-1, the first chromatographic column, 5-2, the second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank.

[0028] Figure 2 Structure diagram of the automated chromatographic column system with pre-column valve in dual-column isocratic mode described in Example 2;

[0029] Wherein: 1, sample tank, 2-1, the first mobile phase storage tank, 2-2, the second mobile phase storage tank, 2-3, the third mobile phase storage tank, 3-1, the first multi-pass solvent selection valve, 3-2, the second multi-pass solvent selection valve, 4-1, the first chromatographic pump, 4-2, the second chromatographic pump, 5-1, the first chromatographic column, 5-2, the second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank, 10, pre-column valve.

[0030] Figure 3 Structure diagram of the automated chromatographic column system in dual-column gradient mode described in Example 3;

[0031] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-A3, A3 mobile phase storage tank, 2-B1, B1 mobile phase storage tank, 2-B2, B2 mobile phase storage tank, 3-1, the first multi-pass solvent selection valve, 3-2, the second multi-pass solvent selection valve, 3-3, the third multi-pass solvent selection valve, 4-1, the first chromatographic pump, 4-2, the second chromatographic pump, 5-1, the first chromatographic column, 5-2, the second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank, 10, pre-column valve, 11-1, the first proportional valve, 11-2, the second proportional valve.

[0032] Figure 4 Structure diagram of the automated chromatographic column system without pre-column valve in three-column isocratic mode described in Example 4;

[0033] Wherein: 1, sample tank, 2-1, the first mobile phase storage tank, 2-2, the second mobile phase storage tank, 2-3, the third mobile phase storage tank, 3-1, the first multi-pass solvent selection valve, 3-2, the second multi-pass solvent selection valve, 3-3, the third multi-pass solvent selection valve, 4-1, the first chromatographic pump, 4-2, the second chromatographic pump, 4-3, the third chromatographic pump, 5-1, the first chromatographic column, 5-2, the second chromatographic column, 5-3, the third chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank.

[0034] Figure 5 Structure diagram of the automated chromatographic column system in three column gradient mode as described in Example 5;

[0035] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-B, B mobile phase storage tank, 3-1, first multi-pass solvent selection valve, 3-2, second multi-pass solvent selection valve, 3-3, third multi-pass solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank, 10-1, first pre-column valve, 10-2, second pre-column valve, 10-3, third pre-column valve, 11-1, first proportional valve, 11-2, second proportional valve.

[0036] Figure 6 Structure diagram of the automated chromatographic column system in four column isocratic mode without pre-column valve as described in Example 6;

[0037] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage, 2-3, third mobile phase storage tank, 3-1, first multi-pass solvent selection valve, 3-2, second multi-pass solvent selection valve, 3-3, third multi-pass solvent selection valve, 3-4, fourth multi-pass solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 4-4, fourth chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 5-4, fourth chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank. Figure 7 Structure diagram of the automated chromatographic column system in four column isocratic mode without pre-column valve and containing two sets of detectors as described in Example 7;

[0038] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 2-3, third mobile phase storage tank, 3-1, first multi-pass solvent selection valve, 3-2, second multi-pass solvent selection valve, 3-3, third multi-pass solvent selection valve, 3-4, fourth multi-pass solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 4-4, fourth chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 5-4, fourth chromatographic column, 6, post-column valve, 7-1, first detector, 7-2, second detector, 8-1, first fraction collector, 8-2, second fraction collector, 9, waste tank.

[0039] Figure 8This is a schematic diagram of the automated chromatographic column system without a column inlet valve in the dual-column isocratic mode with only two mobile phase tanks as described in Example 8;

[0040] The components are: 1. Sample container; 2-1. First mobile phase storage tank; 2-2. Second mobile phase storage tank; 3-1. First multi-port solvent selection valve; 3-2. Second multi-port solvent selection valve; 4-1. First chromatographic pump; 4-2. Second chromatographic pump; 5-1. First chromatographic column; 5-2. Second chromatographic column; 6. Post-column valve; 7. Detector device; 8. Fraction collector; 9. Waste liquid tank.

[0041] Figure 9 This is a schematic diagram of the automated chromatography column system in dual-column gradient mode with dual pumps as described in Example 9;

[0042] The components are: 1. Sample container; 2-1. First mobile phase storage tank; 2-2. Second mobile phase storage tank; 4-1. First chromatographic pump; 4-2. Second chromatographic pump; 4-3. Third chromatographic pump; 5-1. First chromatographic column; 5-2. Second chromatographic column; 6. Post-column valve; 7. Detector device; 8. Fraction collector; 9. Waste liquid tank; 10. Pre-column valve. Detailed Implementation

[0043] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model. For example, changing the type or quantity of valves to achieve the same effect is also within the scope.

[0044] Example 1

[0045] like Figure 1 The automated chromatographic column system without a pre-column valve in dual-column isocratic mode shown includes a sample tank 1, a first mobile phase reservoir 2-1, a second mobile phase reservoir 2-2, a third mobile phase reservoir 2-3, a first multi-port solvent selection valve 3-1, a second multi-port solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, and connecting pipes.

[0046] The sample tank 1 and the first mobile phase tank 2-1, the second mobile phase tank 2-2 and the third mobile phase tank 2-3 are connected in parallel, and connected with the first multi-pass solvent selection valve 3-1 and the second multi-pass solvent selection valve 3-2 connected in parallel through connecting pipes, and the multi-pass solvent selection valves are connected with the first chromatographic pump 4-1 and the second chromatographic pump 4-2 through connecting pipes; the chromatographic pumps are connected with the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipes, the chromatographic columns are connected with the post-column valve 6 through connecting pipes, the post-column valve 6 is connected with the detector device 7 and the waste liquid tank 9 connected in parallel through connecting pipes, and the detector device 7 is connected with the fraction collector 8 through connecting pipes.

[0047] The first mobile phase tank 2-1 is used for elution, the second mobile phase tank 2-2 is used for column washing, and the third mobile phase tank 2-3 is used for balance. According to the process characteristics, there are mainly the following steps: column balance (10 minutes)-sample injection (40 minutes)-elution (40 minutes)-column washing (10 minutes); according to the running time of each process, the adjacent running program combination is configured, the column balance, sample injection and elution, and column washing time are equivalent, and the switching program is divided into two sections, which are: column balance-sample injection and elution-column washing.

[0048] The working process of the chromatographic column system includes the following steps:

[0049] Step 1, the first chromatographic column 5-1 balance: switch the first multi-pass solvent selection valve 3-1 to communicate with the third mobile phase tank 2-3, switch the post-column valve 6 to communicate with the waste liquid tank 9, start the first chromatographic pump 4-1, and balance the first chromatographic column 5-1 according to the time program requirements;

[0050] Step 2, the first chromatographic column 5-1 sample injection: switch the first multi-pass solvent selection valve 3-1 to the sample tank, and the other valves and pumps run as in the previous step, and inject the first chromatographic column 5-1 according to the time program requirements;

[0051] Step 3, the first chromatographic column 5-1 elution-column washing: elution: switch the first multi-pass solvent selection valve 3-1 to the first mobile phase tank 2-1, and switch the post-column valve 6 to the detector device 7. The first chromatographic column 5-1 is eluted, detected by the detector device 7, and the fraction collector 8 collects samples; column washing: after the elution time runs out, switch the first multi-pass solvent selection valve 3-1 to the second mobile phase tank 2-2, and the others remain unchanged, and the first chromatographic column 5-1 is column washed until the end of the time program;

[0052] Step 4, Second Column 5-2 Equilibration-Injection: While running the above steps, simultaneously start this step by starting the second chromatographic pump 4-2. The post-column valve 6 should be in the state where the second chromatographic column 5-2 is connected to the waste tank 9, and the first chromatographic column 5-1 is connected to the detector device 7. Second Column 5-2 Equilibration: Switch the second multi-port solvent selection valve 3-2 to the third mobile phase tank 2-3, and equilibrate the second chromatographic column 5-2 using the second chromatographic pump 4-2. Injection: After the second chromatographic column 5-2 equilibration time is completed, switch the second multi-port solvent selection valve 3-2 to the sample tank 1 and inject the sample into the second chromatographic column 5-2. The program ends at this time.

[0053] Step 5, Cycling: While equilibrating and injecting the first chromatographic column 5-1, the second chromatographic column 5-2 is eluted and washed. The post-column valve 6 is switched to connect the first chromatographic column 5-1 to the waste liquid tank 9, and the equilibration-injection program of the first chromatographic column 5-1 is run through a time program. At this time, the second chromatographic column 5-2 is connected to the detector device 7 through the post-column valve 6, and the detector device 7 is connected to the fraction collector 8. The elution-washing program of the second chromatographic column 5-2 is run through a time program. The two chromatographic columns are equilibrated, injected, eluted, and washed in accordance with the methods in steps 3, 4, and 5.

[0054] Example 2

[0055] like Figure 2 The automated chromatographic column system with a pre-column valve in dual-column isocratic mode shown includes a sample tank, a first mobile phase reservoir 2-1, a second mobile phase reservoir 2-2, a third mobile phase reservoir 2-3, a first multi-port solvent selection valve 3-1, a second multi-port solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, a pre-column valve 10, and connecting pipes.

[0056] Sample container 1 is connected in parallel with first mobile phase storage tank 2-1, second mobile phase storage tank 2-2, and third mobile phase storage tank 2-3. It is connected to first multi-port solvent selection valve 3-1 and second multi-port solvent selection valve 3-2 in parallel via connecting pipes. The multi-port solvent selection valve is connected to column inlet valve 10 via connecting pipes. Column inlet valve 10 is connected to first chromatographic pump 4-1 and second chromatographic pump 4-2 in parallel via connecting pipes. The chromatographic pumps are connected to first chromatographic column 5-1 and second chromatographic column 5-2 respectively via connecting pipes. The chromatographic column is connected to post-column valve 6 via connecting pipes. Post-column valve 6 is connected to detector device 7 and waste liquid tank 9 in parallel via connecting pipes. Detector device 7 is connected to fraction collector 8 via connecting pipes.

[0057] The first mobile phase tank 2-1 is used for elution; the second mobile phase tank 2-2 is used for column washing; and the third mobile phase tank 2-3 is used for balancing. According to the characteristics of the process, there are mainly the following steps: column balancing (10 minutes) - sample injection (15 minutes) - elution (40 minutes) - column washing (10 minutes). According to the running time of each process, adjacent running program combinations are configured, and the column balancing, sample injection and column washing time are basically five minutes shorter than the elution time, which needs to be continuously run. A zero flow rate program is added, and this program is added to the column washing program, so that the program runs with the following programs: column balancing (10 minutes) - sample injection (15 minutes) - elution (40 minutes) - column washing (10 minutes) - zero flow rate (5 minutes). The switching program is divided into two parts: column washing - zero flow rate - column balancing - sample injection and elution.

[0058] The working process of the chromatographic column system includes the following steps:

[0059] Step 1, balancing of the first chromatographic column 5-1: the first multi-pass solvent selection valve 3-1 to the third mobile phase tank 2-3, switching the pre-column valve 10 to make the first multi-pass solvent valve 3-1 connected to the first chromatographic pump 4-1 through the pre-column valve 10, and switching the post-column valve 6 to the waste liquid tank 9. The third mobile phase tank 2-3 is used as the mobile phase, the first chromatographic pump 4-1 is started, and the first chromatographic column 5-1 is balanced according to the time program requirements;

[0060] Step 2, sample injection of the first chromatographic column 5-1: switching the first multi-pass solvent selection valve 3-1 to the sample tank 1, and the other valves and pumps are operated as in the previous step. The first chromatographic column 5-1 is injected according to the time program requirements;

[0061] Step 3, elution of the first chromatographic column 5-1: after the sample injection time is completed, the pre-column valve 10 is switched to connect the first multi-pass solvent valve 3-1 to the second chromatographic column 5-2, and the second multi-pass solvent valve 3-2 is connected to the second chromatographic column 5-1. The post-column valve 6 is switched to connect the first chromatographic column 5-1 to the detector 7 through the post-column valve 6, and finally the sample is collected by the fraction collector 8. The second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6. The second multi-pass solvent valve 3-2 is switched to the first mobile phase tank 2-1, and the first chromatographic pump 4-1 is started to elute the first chromatographic column 5-1 according to the time program.

[0062] Step 4, Second Column 5-2 Wash - Zero Flow Rate - Column Equilibration - Injection: While running the above steps, simultaneously start this step. Since this is the first run of the column, the column has already been washed. This section of the program can be set to zero flow rate (i.e., pump not started), or the column can be washed again. This embodiment uses the second washing method. At this time, keep the pre-column valve 10 and post-column valve 6 unchanged. Column Wash: Start the second chromatographic pump 4-2 and run it according to the washing program. Zero Flow Rate: Switch the first multi-port solvent selection valve 3-1 to the second mobile phase tank 2-2, set the pump not to start, and wait five minutes. Column Equilibration: Switch the first multi-port solvent selection valve 3-1 to the third mobile phase tank 2-3 pipeline, start the first chromatographic pump 4-2, and run it according to the time program of this step. Injection: Switch the first multi-port solvent selection valve 3-1 to the sample tank pipeline, start the second chromatographic pump 4-2, and run it according to the time program of this step.

[0063] Step 5, Cycle: The process involves washing the first column 5-1, achieving zero flow, equilibrating the column, and injecting the sample. Simultaneously, elute the second column 5-2. At the same time, switch the pre-column valve 10 and the post-column valve 6, connecting the first multi-port solvent valve 3-1 to the first chromatographic pump 4-1 via the pre-column valve 10, and then connecting it to the waste tank 9 after passing through the first column 5-1. The second multi-port solvent selection valve 3-2 is connected to the first chromatographic pump 4-2 via the pre-column valve 10, and then connects it to the detector device 7 after passing through the second column 5-2. Finally, the sample is collected through the fraction collector 8. The first column 5-1 washing-zero flow-equilibrating-injection program is run using a time program; simultaneously, the second column 5-2 elution program is run using a time program, and this cycle continues.

[0064] Example 3

[0065] like Figure 3 The automated chromatographic column system in dual-column gradient mode shown includes a sample tank 1, mobile phase reservoirs A1, A2, and A3, mobile phase reservoirs B1 and B2, a first multi-port solvent selection valve 3-1, a second multi-port solvent selection valve 3-2, a third multi-port solvent selection valve 3-3, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first column 5-1, a second column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste tank 9, a pre-column valve 10, a first proportional valve 11-1, a second proportional valve 11-2, and connecting pipes.

[0066] The sample tank 1 and the A1 mobile phase storage tank 2-A1, the A2 mobile phase storage tank 2-A2, the A3 mobile phase storage tank 2-A3, the B1 mobile phase storage tank 2-B1 and the B2 mobile phase storage tank 2-B2 are connected in parallel, and the sample tank 1 and the A1 mobile phase storage tank 2-A1, the A2 mobile phase storage tank 2-A2, the A3 mobile phase storage tank 2-A3, the B1 mobile phase storage tank 2-B1 and the B2 mobile phase storage tank 2-B2 are connected with the first multi-way solvent selection valve 3-1 through a connecting pipe, the A1 mobile phase storage tank 2-A1, the A2 mobile phase storage tank 2-A2 and the A3 mobile phase storage tank 2-A3 are connected with the second multi-way solvent selection valve 3-2, the B1 mobile phase storage tank 2-B1 and the B2 mobile phase storage tank 2-B2 are connected with the third multi-way solvent selection valve 3-3, the first multi-way solvent selection valve 3-1 is connected with the first chromatographic pump 4-1, the second multi-way solvent selection valve 3-2 and the third multi-way solvent selection valve 3-3 are connected with the first proportional valve 11-1 and the second proportional valve 11-2 connected in parallel respectively and then connected with the second chromatographic pump 4-2, the chromatographic pump is then connected with the pre-column valve 10 through a connecting pipe, the pre-column valve 10 is correspondingly connected with the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipes, the chromatographic column is connected with the post-column valve 6 through a connecting pipe, the post-column valve 6 is connected with the detector device 7 and the waste liquid tank 9 connected in parallel through a connecting pipe, and the detector device 7 is connected with the fraction collector 8 through a connecting pipe.

[0067] According to the process characteristics, there are mainly the following steps: column equilibration (A1 mobile phase, 10 minutes) - sample injection (sample injection, 40 minutes) - elution 1 (A2 / B2 mobile phase gradient operation, 20 minutes) - elution 2 (A3 / B2 mobile phase gradient operation, 40 minutes) - column washing (B1 mobile phase, 10 minutes). According to the running time of each process, the adjacent running program combination is configured, and the column washing, column equilibration, sample injection and elution 1 and elution 2 have the same time, and the switching program is divided into two sections, which are: column washing-equilibrium-sample injection and elution 1-elution 2.

[0068] The working process of the chromatographic column system includes the following steps:

[0069] Step 1, column washing of the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the B1 mobile phase storage tank 2-B1, pass through the first chromatographic pump 4-1, and perform column washing on the first chromatographic column 5-1 by the pre-column valve 10, while switching the post-column valve 6 to the waste liquid tank 9, and starting the first chromatographic pump 4-1 to perform column washing on the first chromatographic column 5-1 according to the time program requirement;

[0070] Step 2, column equilibration of the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the A1 mobile phase storage tank 2-A1, and the other valves and pumps operate as in the previous step, and the first chromatographic column 5-1 is equilibrated according to the time program requirement;

[0071] Step 3, sample injection of first column 5-1: switch the first multi-port solvent selection valve 3-1 to the sample tank 1, and the other valves and pumps are the same as the previous step. Sample injection of the first column 5-1 is performed according to the time program;

[0072] Step 4, elution of the first column 5-1 (elution 1-elution 2): elution 1: switch the pre-column valve 10 to connect the second chromatographic pump 4-2 with the first column 5-1, and the first chromatographic pump 4-1 with the second column 5-2. Switch the post-column valve 6 to connect the first column 5-1 with the detector device 7, and the second column 5-2 with the waste tank 9. Switch the second multi-port solvent selection valve 3-2 to connect with the mobile phase storage tank 2-A2, and the third multi-port solvent selection valve 3-3 to connect with the mobile phase storage tank 2-B2. Run the elution 1 program according to the time program. Elution 2: keep the pre-column valve 10 and the post-column valve 6 unchanged, switch the second multi-port solvent selection valve 3-2 to connect with the mobile phase storage tank 2-A3, and the third multi-port solvent selection valve 3-3 to connect with the mobile phase storage tank 2-B2. Run the elution 2 program according to the time program. Finally, collect the sample through the fraction collector 8;

[0073] Step 5, column washing-equilibrium-sample injection of the second column 5-2: keep the pre-column valve 10 and the post-column valve 6 unchanged as in the previous step. Run the current step at the same time as the above step. Column washing: switch the first multi-port solvent selection valve 3-1 to the B1 mobile phase storage tank 2-B1, and perform column washing of the second column 5-2 through the first chromatographic pump 4-1 according to the time program. Equilibrium: switch the first multi-port solvent selection valve 3-1 to the A1 mobile phase storage tank 2-A1, and perform equilibrium of the second column 5-2 through the first chromatographic pump 4-1 according to the time program. Sample injection: switch the first multi-port solvent selection valve 3-1 to the sample tank 1, and perform sample injection of the second column 5-2 through the first chromatographic pump 4-1 according to the time program.

[0074] Step 6, cycle: after completing the above two steps, switch the pre-column valve 10 and the post-column valve 6 at the same time, and perform column washing-equilibrium-sample injection of the first column 5-1 and elution 1-elution 2 of the second column 5-2 according to the time program. Repeat the above steps for the two columns.

[0075] Example 4

[0076] As Figure 4The illustrated three-column isocratic mode without pre-column valve automated chromatographic column system includes a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a third mobile phase storage tank 2-3, a first multi-pass solvent selection valve 3-1, a second multi-pass solvent selection valve 3-2, a third multi-pass solvent selection valve 3-3, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a third chromatographic pump 4-3, a first chromatographic column 5-1, a second chromatographic column 5-2, a third chromatographic column 5-3, a post-column valve 6, a detector device 7, a fraction collector 8, a waste tank 9 and connecting pipes.

[0077] The sample tank 1 and the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2 and the third mobile phase storage tank 2-3 are connected in parallel and connected to the parallel first multi-pass solvent selection valve 3-1, the second multi-pass solvent selection valve 3-2 and the third multi-pass solvent selection valve 3-3 through connecting pipes, and the multi-pass solvent selection valves are connected to the first chromatographic pump 4-1, the second chromatographic pump 4-2 and the third chromatographic pump 4-3 through connecting pipes; the chromatographic pumps are connected to the first chromatographic column 5-1, the second chromatographic column 5-2 and the third chromatographic column 5-3 through connecting pipes, and the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and waste tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0078] According to the process characteristics, there are mainly the following steps: column equilibration (15 minutes) - sample injection (40 minutes) - elution (40 minutes) - column washing (15 minutes); according to the running time of each process, configure the adjacent running program combination, the column washing, column equilibration, sample injection and elution three sections of time are equivalent, adjust the column washing and column equilibration time to be 20 minutes, divide the switching program into three sections, which are: column washing (20 minutes) - column equilibration (20 minutes), sample injection (40 minutes) and elution (40 minutes); 1, the sample tank, 2-1 tank is used for elution, 2-2 tank is used for column washing, and 2-3 tank is used for equilibration.

[0079] The working process of the chromatographic column system includes the following steps:

[0080] Step 1, the first chromatographic column 5-1 is washed and equilibrated.

[0081] The first chromatographic column 5-1 is washed and equilibrated: switch the first multi-pass solvent selection valve 3-1 to the second mobile phase storage tank 2-2, switch the post-column valve 6 to the waste tank 9, use the second mobile phase storage tank 2-2, start the first chromatographic pump 4-1, and wash the first chromatographic column 5-1 according to the time program requirements; the first chromatographic column 5-1 is equilibrated: switch the first multi-pass solvent selection valve 3-1 to the third mobile phase storage tank 2-3, keep the post-column valve 6 switched to the waste tank 9, use the equilibration mobile phase, start the first chromatographic pump 4-1, and equilibrate the chromatographic column first chromatographic column 5-1 according to the time program requirements;

[0082] Step 2, first chromatographic column 5-1 injection, second chromatographic column 5-2 column washing-chromatographic column balance.

[0083] First chromatographic column 5-1 injection: switch the first multi-pass solvent selection valve 3-1 to the sample tank 1, and the other valves and pumps continue to operate as in the previous step. The first chromatographic column 5-1 is injected according to the time program; second chromatographic column 5-2 column washing-chromatographic column balance: while the first chromatographic column 5-1 is being injected, start the second chromatographic pump 4-2, and switch the second multi-pass solvent selection valve 3-2 to perform column washing-chromatographic column balance on the second chromatographic column 5-2 according to the time program;

[0084] Step 3, first chromatographic column 5-1 elution, second chromatographic column 5-2 injection, third chromatographic column 5-3 column washing-chromatographic column balance.

[0085] First chromatographic column 5-1 elution: switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7, switch the first multi-pass solvent selection valve 3-1 to the first mobile phase storage tank 2-1, and operate the first chromatographic pump 4-1. The first chromatographic column 5-1 is eluted according to the time program, and finally the sample is collected by the fraction collector 8; second chromatographic column 5-2 injection: while the above step is being performed, start the second chromatographic pump 4-2, and switch the second multi-pass solvent selection valve 3-2 to the sample tank 1. The second chromatographic column 5-2 is injected according to the time program; third chromatographic column 5-3 column washing-chromatographic column balance: while the first chromatographic column 5-1 is being eluted and the second chromatographic column 5-2 is being injected, start the third chromatographic pump 4-3, and switch the third multi-pass solvent selection valve 3-3 to perform column washing-chromatographic column balance on the third chromatographic column 5-3 according to the time program;

[0086] Step 4, first chromatographic column 5-1 column washing-chromatographic column balance, second chromatographic column 5-2 elution, second chromatographic column 5-3 injection.

[0087] First chromatographic column 5-1 column washing-chromatographic column balance: when the first chromatographic column 5-1 elution time program is completed, switch the post-column valve 6 to connect the second chromatographic column 5-2 to the detector device 7, start the first chromatographic pump 4-1, and switch the first multi-pass solvent selection valve 3-1 to perform column washing-chromatographic column balance on the first chromatographic column 5-1 according to the time program; second chromatographic column 5-2 elution: while the above step is being performed, start the second chromatographic pump 4-2, and switch the second multi-pass solvent selection valve 3-2 to the mobile phase storage tank 2-1. The second chromatographic column 5-2 is eluted according to the time program, and finally the sample is collected by the fraction collector 8; third chromatographic column 5-3 injection: while the above step is being performed, start the third chromatographic pump 4-3, and switch the third multi-pass solvent selection valve 3-3 to the sample tank 1. The third chromatographic column 5-3 is injected according to the time program;

[0088] Step 5, first column 5-1 injection, second column 5-2 column washing-column equilibration, third column 5-3 column elution.

[0089] First column 5-1 injection: when the above step 4 is running, switch the post-column valve 6 to connect the third column 5-3 with the detector device 7, start the first chromatographic pump 4-1, and switch the first multi-port solvent selection valve 3-1 according to the time program to inject the first column 5-1; Second column 5-2 column washing-column equilibration: at the same time as running the above step, start the second chromatographic pump 4-2, and switch the second multi-port solvent selection valve 3-2 to the mobile phase storage tank 2-2, the mobile phase storage tank 2-3 according to the time program requirements to wash and equilibrate the second column 5-2; Third column 5-3 column elution: at the same time as running the above step, start the third chromatographic pump 4-3, switch the third multi-port solvent selection valve 3-3 to the mobile phase storage tank 2-1, and elute the third column 5-3 according to the time program requirements, and finally collect the sample through the fraction collector 8;

[0090] Step 6, cycle: according to the above three steps, the three columns are cycled for elution-column equilibration-injection-column washing.

[0091] Example 5

[0092] As shown in the three-column gradient mode automatic chromatographic column system, comprising Figure 5

[0093] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-B, B mobile phase storage tank, 3-1, first multi-port solvent selection valve, 3-2, second multi-port solvent selection valve, 3-3, third multi-port solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump(high precision pump), 5-1, first column, 5-2, second column, 5-3, third column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste tank, 10-1, first pre-column valve, 10-2, second pre-column valve, 10-3, third pre-column valve, 11-1, first proportional valve, 11-2, second proportional valve and connecting pipe.

[0094] ​The sample tank 1 and the A1 mobile phase storage tank 2-A1, the A2 mobile phase storage tank 2-A2, and the B1 mobile phase storage tank 2-B are connected in parallel, and then connected with the first multi-pass solvent selection valve 3-1 and the second multi-pass solvent selection valve 3-2 connected in parallel, and the first multi-pass solvent selection valve 3-1 and the second multi-pass solvent selection valve 3-2 are respectively connected with the first chromatographic pump 4-1 and the second chromatographic pump 4-2 connected in parallel; the A1 mobile phase storage tank 2-A1 and the A2 mobile phase storage tank 2-A2 are connected with the third multi-pass solvent selection valve 3-3, the third multi-pass solvent selection valve 3-3 is connected with the first proportional valve 11-1, the B1 mobile phase storage tank 2-B is connected with the second proportional valve 11-2, and the proportional valve is connected with the third chromatographic pump 4-3; the chromatographic pump is then connected with the first pre-column valve 10-1, the second pre-column valve 10-2, and the third pre-column valve 10-3 connected in parallel through a connecting pipe, the pre-column valve 10 is connected with the first chromatographic column 5-1, the second chromatographic column 5-2, and the third chromatographic column 5-3 through a connecting pipe, the chromatographic column is connected with the post-column valve 6 through a connecting pipe, and the post-column valve 6 is connected with the detector device 7 and the waste tank 9 connected in parallel through a connecting pipe, and the detector device 7 is connected with the fraction collector 8 through a connecting pipe.

[0095] According to the process characteristics, there are mainly the following steps: column equilibration (first mobile phase storage tank 2-A1, 15 minutes)-chromatographic sampling (sample tank 1, 40 minutes)-elution (second mobile phase storage tank 2-A2 / third mobile phase storage tank 2-B gradient operation, 40 minutes)-column washing (third mobile phase storage tank 2-B, 15 minutes); according to the running time of each process, the adjacent running program combination is configured, and the switching program is divided into three sections, which are column washing-equilibrium, sampling, and elution. In order to switch three columns, the running time of each section is adjusted to be the same, and the adjustment is as follows: column washing (20 minutes)-equilibrium (20 minutes); sampling (40 minutes); elution (40 minutes). Elution program: when the chromatographic column runs the elution program, the third chromatographic pump 4-3 (high-precision pump) is used to connect the detector device through the post-column valve 6.

[0096] Step 1, column washing and equilibration of the first chromatographic column 5-1:

[0097] The first multi-pass solvent selection valve 3-1 is switched to the third mobile phase storage tank 2-B, the first chromatographic pump 4-1 is started, the first chromatographic column 5-1 is column-washed through the first pre-column valve 10-1 according to the time program, the first pre-column valve 10-1 is kept unchanged, the first multi-pass solvent selection valve 3-1 is switched to the first mobile phase storage tank 2-A1, and the column is equilibrated according to the equilibration time program;

[0098] Step 2, column washing and equilibration of the second chromatographic column 5-2, and sampling of the first chromatographic column 5-1

[0099] Switch the first multi-pass solvent selection valve 3-1 to the third mobile phase reservoir 2-B, start the first chromatographic pump 4-1, and wash the third chromatographic column 5-3 through the first pre-column valve 10-1 according to the time program. Keep the first pre-column valve 10-1 unchanged, switch the first multi-pass solvent selection valve 3-1 to the first mobile phase reservoir 2-A1, and balance the chromatographic column according to the balance time program;

[0100] At the same time of running the above operation, switch the second multi-pass solvent selection valve 3-2 to the sample tank 1, start the second chromatographic pump 4-2, and inject the sample into the first chromatographic column 5-1 through the second pre-column valve 10-2 according to the time program;

[0101] Step 3, column washing and balancing of the third chromatographic column 5-3, sample injection of the second chromatographic column 5-2, and elution of the first chromatographic column 5-1

[0102] Switch the first multi-pass solvent selection valve 3-1 to the third mobile phase reservoir 2-B, start the first chromatographic pump 4-1, and wash the third chromatographic column 5-3 through the first pre-column valve 10-1 according to the time program. Keep the first pre-column valve 10-1 unchanged, switch the first multi-pass solvent selection valve 3-1 to the first mobile phase reservoir 2-A1, and balance the chromatographic column according to the balance time program;

[0103] At the same time of running the above operation, switch the second multi-pass solvent selection valve 3-2 to the sample tank 1, start the second chromatographic pump 4-2, and inject the sample into the second chromatographic column 5-2 through the second pre-column valve 10-2 according to the time program;

[0104] At the same time of running the above operation, switch the third multi-pass solvent selection valve 3-3 to the second mobile phase reservoir 2-A2, start the third chromatographic pump 4-3, and elute the first chromatographic column 5-1 through the third pre-column valve 10-3 according to the time program. At this time, the post-column valve 6 is switched to be connected to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0105] Step 4, sample injection of the third chromatographic column 5-3, elution of the second chromatographic column 5-2, and column washing and balancing of the first chromatographic column 5-1

[0106] Switch the second multi-pass solvent selection valve 3-2 to the sample tank 1, start the second chromatographic pump 4-2, and inject the sample into the third chromatographic column 5-3 through the second pre-column valve 10-2 according to the time program;

[0107] At the same time of running the above operation, switch the third multi-pass solvent selection valve 3-3 to the second mobile phase reservoir 2-A2, start the third chromatographic pump 4-3, and elute the second chromatographic column 5-2 through the third pre-column valve 10-3 according to the time program. At this time, the post-column valve 6 is switched to be connected to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0108] While performing the above operations, switch the first multi-channel solvent selection valve 3-1 to the third mobile phase reservoir 2-B, start the first chromatographic pump 4-1, and wash the first chromatographic column 5-1 according to the time program through the first column inlet valve 10-1. Keep the first column inlet valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase reservoir 2-A1, and equilibrate the chromatographic column according to the equilibration time program;

[0109] Step 5: Elution on the third column (5-3), washing and equilibration on the second column (5-2), injection on the first column (5-1).

[0110] Switch the third multi-channel solvent selection valve 3-3 to the second mobile phase storage tank 2-A2, start the third chromatographic pump 4-3, and elute the third chromatographic column 5-3 according to the time program through the third column pre-valve 10-3. At this time, the post-column valve 6 is switched to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0111] While performing the above operations, switch the first multi-channel solvent selection valve 3-1 to the third mobile phase reservoir 2-B, start the first chromatographic pump 4-1, and wash the second chromatographic column 5-2 according to the time program through the first column inlet valve 10-1. Keeping the first column inlet valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase reservoir 2-A1, and equilibrate the chromatographic column according to the equilibration time program; while performing the above operations, switch the second multi-channel solvent selection valve 3-2 to the sample container 1, start the second chromatographic pump 4-2, and inject the sample into the first chromatographic column 5-1 according to the time program through the second column inlet valve 10-2.

[0112] Cycling: The three chromatographic columns were cyclically washed, equilibrated, loaded, and eluted according to the above method.

[0113] Example 6

[0114] like Figure 6 The automated column system without a column initiation valve shown in the four-column isocratic mode includes...

[0115] Sample container 1, first mobile phase storage tank 2-1, second mobile phase storage tank 2-2, third mobile phase storage tank 2-3, first multi-port solvent selection valve 3-1, second multi-port solvent selection valve 3-2, third multi-port solvent selection valve 3-3, fourth multi-port solvent selection valve 3-4, first chromatographic pump 4-1, second chromatographic pump 4-2, third chromatographic pump 4-3, fourth chromatographic pump 4-4, first chromatographic column 5-1, second chromatographic column 5-2, third chromatographic column 5-3, fourth chromatographic column 5-4, post-column valve 6, detector device 7, fraction collector 8, waste liquid tank 9, and connecting pipes.

[0116] The sample tank 1 and the first, second and third mobile phase storage tanks 2-1, 2-2 and 2-3 are connected in parallel and connected to the first, second, third and fourth multi-pass solvent selection valves 3-1, 3-2, 3-3 and 3-4 through connecting pipes, the multi-pass solvent selection valves are connected to the first, second, third and fourth chromatographic pumps 4-1, 4-2, 4-3 and 4-4 through connecting pipes, the chromatographic pumps are connected to the first, second, third and fourth chromatographic columns 5-1, 5-2, 5-3 and 5-4 through connecting pipes, the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the detector device 7 and the waste liquid tank 9 in parallel through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through a connecting pipe.

[0117] According to the process characteristics, there are mainly the following steps: chromatographic column equilibration (25 minutes)-sample injection (30 minutes)-elution (30 minutes)-column washing (25 minutes). According to the running time of each process, the running time is adjusted, and the switching program is divided into four sections, which are chromatographic column equilibration (30 minutes), sample injection (30 minutes), elution (30 minutes) and column washing (30 minutes) respectively.

[0118] When the chromatographic column elution program is running, the post-column valve 6 is switched to connect the chromatographic column to the detector device 7, and the sample is collected through the fraction collector 8.

[0119] Step 1, equilibration of the first chromatographic column 5-1

[0120] The first solvent selection valve 3-1 is switched to connect the third mobile phase storage tank 2-3, the post-column valve 6 is switched to connect the waste liquid tank 9, the equilibration mobile phase is used, the first chromatographic pump 4-1 is started, and the first chromatographic column 5-1 is equilibrated according to the time program requirements.

[0121] Step 2, sample injection of the first chromatographic column 5-1 and equilibration of the second chromatographic column 5-2

[0122] The first solvent selection valve 3-1 is switched to connect the sample tank 1, the post-column valve 6 is switched to connect the first chromatographic column 5-1 to the waste liquid tank 9, the first chromatographic pump 4-1 is started, and the first chromatographic column 5-1 is injected according to the time program requirements.

[0123] At the same time as the above operation, the second solvent selection valve 3-2 is switched to connect the third mobile phase storage tank 2-3, the equilibration mobile phase is used, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is equilibrated according to the time program requirements.

[0124] Step 3, elution of the first chromatographic column 5-1, sample injection of the second chromatographic column 5-2 and equilibration of the third chromatographic column 5-3

[0125] Switch the first solvent selection valve 3-1 to connect the first mobile phase reservoir 2-1, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7, finally use the fraction collector 8 to collect the sample, use the elution mobile phase, start the first chromatographic pump 4-1, and elute the first chromatographic column 5-1 according to the time program requirements;

[0126] At the same time of running the above operation, switch the second solvent selection valve 3-2 to connect the sample injection tank 1, switch the post-column valve 6 to connect the second chromatographic column 5-2 to the waste tank 9, start the second chromatographic pump 4-2, and inject the sample into the second chromatographic column 5-2 according to the time program requirements;

[0127] At the same time of running the above operation, switch the third solvent selection valve 3-3 to connect the third mobile phase reservoir 2-3, use the equilibration mobile phase, start the third chromatographic pump 4-3, and equilibrate the third chromatographic column 5-3 according to the time program requirements;

[0128] Step 4, the first chromatographic column 5-1 is eluted, the second chromatographic column 5-2 is eluted, the third chromatographic column 5-3 is injected, and the fourth chromatographic column 5-4 is equilibrated Switch the first multi-pass solvent selection valve 3-1 to connect the second mobile phase reservoir 2-2, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the waste tank 9, use the second mobile phase reservoir 2-2, start the first chromatographic pump 4-1, and elute the first chromatographic column 5-1 according to the time program requirements;

[0129] At the same time of running the above operation, switch the second multi-pass solvent selection valve 3-2 to connect the first mobile phase reservoir 2-1, switch the post-column valve to connect the second chromatographic column 5-2 to the detector device 7, finally use the fraction collector 8 to collect the sample, use the elution mobile phase, start the second chromatographic pump 4-2, and elute the second chromatographic column 5-2 according to the time program requirements;

[0130] At the same time of running the above operation, switch the third multi-pass solvent selection valve 3-3 to the sample injection tank 1, switch the post-column valve 6 to connect the third chromatographic column 5-3 to the waste tank 9, start the third chromatographic pump 4-3, and inject the sample into the third chromatographic column 5-3 according to the time program requirements;

[0131] At the same time of running the above operation, switch the fourth multi-pass solvent selection valve 3-4 to connect the mobile phase reservoir 2-3, use the equilibration mobile phase, start the fourth chromatographic pump 4-4, and equilibrate the fourth chromatographic column 5-4 according to the time program requirements;

[0132] Step 5, the first chromatographic column 5-1 is equilibrated, the second chromatographic column 5-2 is eluted, the third chromatographic column 5-3 is eluted, and the fourth chromatographic column 5-4 is injected Switch the first solvent selection valve 3-1 to connect the mobile phase reservoir 2-3, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the waste tank 9, use the equilibration mobile phase, start the first chromatographic pump 4-1, and equilibrate the first chromatographic column 5-1 according to the time program requirements;

[0133] At the same time, switch the second multi-way solvent selection valve 3-2 to connect the second mobile phase tank 2-2, switch the post-column valve 6 to connect the second column 5-2 with the waste tank 9, use the column washing mobile phase, start the second pump 4-2, and follow the time program to wash the second column 5-2;

[0134] At the same time, switch the third multi-way solvent selection valve 3-3 to connect the second mobile phase tank 2-2, switch the post-column valve 6 to connect the third column 5-3 with the detector device 7, use the fraction collector 8 to collect the sample, use the elution mobile phase, start the third pump 4-3, and follow the time program to elute the third column 5-3;

[0135] At the same time, switch the fourth multi-way solvent selection valve 3-4 to connect the first mobile phase tank 2-1, start the fourth pump 4-4, and follow the time program to inject the sample into the fourth column 5-4;

[0136] Step 6, inject the sample into the first column 5-1, switch the first multi-way solvent selection valve 3-1 to connect the sample tank 1, switch the post-column valve 6 to connect the first column 5-1 with the waste tank 9, start the first pump 4-1, and follow the time program to inject the sample into the first column 5-1;

[0137] At the same time, switch the second multi-way solvent selection valve 3-2 to connect the mobile phase tank 2-3, switch the post-column valve 6 to connect the second column 5-2 with the waste tank 9, start the second pump 4-2, and follow the time program to balance the second column 5-2;

[0138] At the same time, switch the third multi-way solvent selection valve 3-3 to connect the second mobile phase tank 2-2, switch the post-column valve 6 to connect the third column 5-3 with the waste tank 9, start the third pump 4-3, and follow the time program to wash the third column 5-3;

[0139] At the same time, switch the fourth multi-way solvent selection valve 3-4 to connect the first mobile phase tank 2-1, switch the post-column valve 6 to connect the fourth column 5-4 with the detector device 7, use the fraction collector 8 to collect the sample, start the fourth pump 4-4, and follow the time program to elute the fourth column 5-4;

[0140] Step 7, elution of the first column 5-1, sample injection of the second column 5-2, equilibration of the third column 5-3, column washing of the fourth column 5-4, switching the first solvent selection valve 3-1 to connect the first mobile phase tank 2-1, switching the post column valve 6 to connect the first chromatographic pump 4-1 to the detector device 7, using the fraction collector 8 to collect the sample, using the elution mobile phase, starting the first chromatographic pump 4-1. Elution of the first column 5-1 according to the time program requirements;

[0141] At the same time as running the above operation, switching the second solvent selection valve 3-2 to connect the sample tank 1, switching the post column valve 6 to connect the second chromatographic column 5-2 to the waste tank 9, starting the second chromatographic pump 4-2, sample injection of the second column 5-2 according to the time program requirements;

[0142] At the same time as running the above operation, switching the third solvent selection valve 3-3 to connect the mobile phase storage tank 2-3, switching the post column valve 6 to connect the third chromatographic column 5-3 to the waste tank 9, starting the third chromatographic pump 4-3, equilibration of the third column 5-3 according to the time program requirements;

[0143] At the same time as running the above operation, switching the fourth solvent selection valve 3-4 to connect the second mobile phase storage tank 2-2, switching the post column valve 6 to connect the fourth chromatographic column 5-4 to the waste tank 9, starting the fourth chromatographic pump 4-4, column washing of the fourth column 5-4 according to the time program requirements;

[0144] Step 8, cycle: according to the above four steps, the four columns are cycled for column equilibration-sample injection-elution-column washing, and the sample is collected.

[0145] Example 7

[0146] As shown in the four-column isocratic mode without pre-column valve and with two sets of detectors, Figure 7

[0147] sample tank 1, first mobile phase storage tank 2-1, second mobile phase storage 2-2, third mobile phase storage tank 2-3, first multi-port solvent selection valve 3-1, second multi-port solvent selection valve 3-2, third multi-port solvent selection valve 3-3, fourth multi-port solvent selection valve 3-4, first chromatographic pump 4-1, second chromatographic pump 4-2, third chromatographic pump 4-3, fourth chromatographic pump 4-4, first chromatographic column 5-1, second chromatographic column 5-2, third chromatographic column 5-3, fourth chromatographic column 5-4, post column valve 6, first detector 7-1, second detector 7-8, first fraction collector 8-1, second fraction collector 8-2, waste tank 9 and connecting pipes.

[0148] ​The sample tank 1 and the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2 and the third mobile phase storage tank 2-3 are connected in parallel, and are connected with the first multi-pass solvent selection valve 3-1, the second multi-pass solvent selection valve 3-2, the third multi-pass solvent selection valve 3-3 and the fourth multi-pass solvent selection valve 3-4 in parallel through connecting pipes, and the multi-pass solvent selection valves are connected with the first chromatographic pump 4-1, the second chromatographic pump 4-2, the third chromatographic pump 4-3 and the fourth chromatographic pump 4-4 through connecting pipes; the chromatographic pumps are connected with the first chromatographic column 5-1, the second chromatographic column 5-2, the third chromatographic column 5-3 and the fourth chromatographic column 5-4 through connecting pipes, and the chromatographic columns are connected with the post-column valve 6 through connecting pipes, and the post-column valve 6 is connected with the first detector 7-1, the second detector 7-2 and the waste liquid tank 9 in parallel through connecting pipes, and the first detector 7-1 and the second detector 7-2 are connected with the first fraction collector 8-1 and the second fraction collector 8-2 respectively through connecting pipes.

[0149] According to the process characteristics, there are mainly the following steps: chromatographic column equilibration (third mobile phase storage tank 2-3, 10 minutes) - sample injection (sample tank 1, 40 minutes) - elution (first mobile phase storage tank 2-1, 60 minutes) - column washing (second mobile phase storage tank 2-2, 10 minutes), the elution time is relatively long, and two sets of detector devices and fraction collectors are configured. According to the running time of each process, the switching program is divided into two parts, which are: column washing (10 minutes) - chromatographic column equilibration (10 minutes) - sample injection (40 minutes) and elution (60 minutes);

[0150] Elution program: when the chromatographic column runs the elution program, the post-column valve 6 is connected with the detector device (7-1, 7-2), and the sample is collected through the fraction collector.

[0151] The working process of the chromatographic column system includes the following steps:

[0152] Step 1, the first chromatographic column 5-1 is column-washed, the chromatographic column is equilibrated, the sample is injected, the third chromatographic column 5-3 is column-washed, the chromatographic column is equilibrated and the sample is injected, the first chromatographic pump 4-1 is started to operate the first chromatographic column 5-1, the first multi-pass solvent selection valve 3-1 is switched to the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column equilibration and the sample tank 1 for sample injection according to the time program, and the first chromatographic column 5-1 is connected with the waste liquid tank 9 through the post-column valve 6;

[0153] Meanwhile, the third chromatographic pump 4-3 is started to operate the first chromatographic column 5-3, the third multi-pass solvent selection valve 3-3 is switched to the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column equilibration and the sample tank 1 for sample injection according to the time program, and the third chromatographic column 5-3 is connected with the waste liquid tank 9 through the post-column valve 6;

[0154] Step 2: Elute from column 5-1, elute from column 5-3, elute from column 5-2, equilibrate from column 5-2, inject the sample; elute from column 5-2, equilibrate from column 5-3, inject the sample.

[0155] Start the first chromatographic pump 4-1 to elute the first chromatographic column 5-1, switch the first multi-channel solvent selection valve 3-1 to connect the first mobile phase storage tank 2-1 to elute according to the time program, the first chromatographic column 5-1 is connected to the first detector device 7-1 through the post-column valve 6, and the sample is collected through the first fraction collector 8-1.

[0156] Simultaneously, the third chromatographic pump 4-3 is started to elute the third chromatographic column 5-3, and the third multi-channel solvent selection valve 3-3 is switched to connect the first mobile phase storage tank 2-1 for elution according to the time program. The third chromatographic column 5-3 is connected to the first detector device 7-2 through the post-column valve 6, and the sample is collected through the first fraction collector 8-2.

[0157] Simultaneously, the second chromatographic pump 4-2 is started to operate the second chromatographic column 5-2, and the second multi-channel solvent selection valve 3-2 is switched to connect the second mobile phase tank 2-2 for column washing, the third mobile phase tank 2-3 for column equilibration, and the sample tank 1 for sample injection according to the time program. The second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6.

[0158] Simultaneously, the fourth chromatographic pump 4-4 is started to operate the fourth chromatographic column 5-4, and the fourth multi-channel solvent selection valve 3-4 is switched to connect the second mobile phase tank 2-2 for column washing, the third mobile phase tank 2-3 for column equilibration, and the sample tank 1 for sample injection according to the time program. The fourth chromatographic column 5-4 is connected to the waste liquid tank 9 through the post-column valve 6.

[0159] Step 3: Wash column 5-1, equilibrate column, inject sample; wash column 5-3, equilibrate column, inject sample; elute column 5-2, elute column 5-2; start the first chromatographic pump 4-1; switch the first multi-channel solvent selection valve 3-1 to connect to the second mobile phase tank 2-2 for washing column, the third mobile phase tank 2-3 for equilibration column, and sample tank 1 for injection according to the time program; the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6.

[0160] Simultaneously, start the third chromatographic pump 4-3, switch the third multi-channel solvent selection valve 3-3 to wash the column in the second mobile phase tank 2-2 according to the time program, balance the chromatographic column in the third mobile phase tank 2-3, and inject the sample tank 1. The third chromatographic column 5-3 is connected to the waste liquid tank 9 through the post-column valve 6.

[0161] At the same time, the second chromatographic pump 4-2 is started, the second multi-channel solvent selection valve 3-2 is switched to connect the first mobile phase storage tank 2-1 to perform elution according to a time program, the second chromatographic column 5-2 is connected to the first detector device 7-1 through the post-column valve 6, and sample collection is performed through the first fraction collector 8-1.

[0162] At the same time, the fourth chromatographic pump 4-4 is started, the fourth multi-channel solvent selection valve 3-4 is switched to connect the first mobile phase storage tank 2-1 to perform elution according to a time program, the fourth chromatographic column 5-4 is connected to the second detector device 7-2 through the post-column valve 6, and sample collection is performed through the second fraction collector 8-2.

[0163] Step 4, cycle: according to the above two steps, the four chromatographic columns are cycled for column equilibration, sample injection, elution, and column washing, and sample collection.

[0164] Example 8

[0165] As Figure 8 described above, the automatic chromatographic column system without a pre-column valve in a two-column isocratic mode includes a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a first multi-channel solvent selection valve 3-1, a second multi-channel solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste tank 9, and connecting pipelines.

[0166] The sample tank 1, the first mobile phase storage tank 2-1, and the second mobile phase storage tank 2-2 are connected in parallel and connected to the parallel first multi-channel solvent selection valve 3-1 and the second multi-channel solvent selection valve 3-2 through connecting pipelines, the multi-channel solvent selection valves are connected to the first chromatographic pump 4-1 and the second chromatographic pump 4-2 through connecting pipelines, respectively, the chromatographic pumps are connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipelines, respectively, the chromatographic columns are connected to the post-column valve 6 through connecting pipelines, the post-column valve 6 is connected to the parallel detector device 7 and waste tank 9 through connecting pipelines, and the detector device 7 is connected to the fraction collector 8 through a connecting pipeline.

[0167] According to the process characteristics, there are mainly the following steps: column equilibration (first mobile phase storage tank 2-1, 10 minutes)-sample injection (sample tank 1, 20 minutes)-elution (second mobile phase storage tank 2-2, 40 minutes)-column washing (second mobile phase storage tank 2-2, 10 minutes). According to the running time of each process, the switching program is divided into two parts: column washing (10 minutes)-column equilibration (10 minutes)-sample injection (20 minutes) and elution (40 minutes).

[0168] The working process of the chromatographic column system includes the following steps:

[0169] Step 1, the first chromatographic column 5-1 is washed, and the sample is injected

[0170] The first chromatographic pump 4-1 is started to operate the first chromatographic column 5-1, the first multi-channel solvent selection valve 3-1 is switched to the second mobile phase storage tank 2-2 according to a time program to wash the column, the first mobile phase storage tank 2-1 is balanced, and the sample tank 1 is injected, and the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6;

[0171] Step 2, the first chromatographic column 5-1 is eluted, and the second chromatographic column 5-2 is washed, balanced and injected:

[0172] The first chromatographic pump 4-1 is started, and the first multi-channel solvent selection valve 3-1 is switched to connect the second mobile phase storage tank 2-2 according to a time program to elute the column, the first chromatographic column 5-1 is connected to the detector device 7 through the post-column valve 6, and sample collection is performed through the fraction collector 8;

[0173] The second chromatographic pump 4-2 is started at the same time, and the second multi-channel solvent selection valve 3-2 is switched to connect the second mobile phase storage tank 2-2 according to a time program to wash the column, the first mobile phase storage tank 2-1 is balanced, and the sample tank 1 is injected, and the second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6;

[0174] Step 3, the first chromatographic column 5-1 is washed, balanced and injected, and the second chromatographic column 5-2 is eluted

[0175] The first chromatographic pump 4-1 is started, and the first multi-channel solvent selection valve 3-1 is switched to connect the second mobile phase storage tank 2-2 according to a time program to wash the column, the first mobile phase storage tank 2-1 is balanced, and the sample tank 1 is injected, and the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6;

[0176] The second chromatographic pump 4-2 is started at the same time, and the second multi-channel solvent selection valve 3-2 is switched to connect the second mobile phase storage tank 2-2 according to a time program to elute the column, the second chromatographic column 5-2 is connected to the detector device 7 through the post-column valve 6, and sample collection is performed through the fraction collector 8;

[0177] Step 4, cycle: according to the above two steps, the two chromatographic columns are cycled to balance the chromatographic column, inject, elute and wash the column, and collect the sample.

[0178] Example 9

[0179] As Figure 9The double-pump gradient double-column gradient mode automatic chromatographic column system comprises a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a third chromatographic pump 4-3, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste tank 9, a pre-column valve 10 and connecting pipelines.

[0180] The sample tank 1, the first mobile phase storage tank 2-1 and the second mobile phase storage tank 2-2 are connected in parallel, the sample tank 1 is connected to the first chromatographic pump 4-1 and then connected to the pre-column valve 10, the first mobile phase storage tank 2-1 and the second mobile phase storage tank 2-2 are respectively connected to the second chromatographic pump 4-2 and the third chromatographic pump 4-3, the second chromatographic pump 4-2 and the third chromatographic pump 4-3 are connected through connecting pipelines and then connected to the pre-column valve 10; the pre-column valve 10 is connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipelines, the chromatographic columns are connected to the post-column valve 6 through connecting pipelines, the post-column valve 6 is connected to the parallel detector device 7 and waste tank 9 through connecting pipelines, and the detector device 7 is connected to the fraction collector 8 through connecting pipelines.

[0181] According to the process characteristics, the following steps are mainly included: column equilibration (10% first mobile phase storage tank 2-1 / 90% second mobile phase storage tank 2-2, 10 minutes) - sample injection (sample tank 1, 50 minutes) - elution (60% first mobile phase storage tank 2-1 / 40% second mobile phase storage tank 2-2, 30 minutes) - column washing (80% first mobile phase storage tank 2-1 / 20% second mobile phase storage tank 2-2, 10 minutes). According to the running time of each process, the switching program is divided into two parts, which are sample injection (50 minutes) and elution (30 minutes) - column washing (10 minutes) - column equilibration (10 minutes). Different proportions of mobile phase are operated at different flow rates by two pumps.

[0182] The working process of the chromatographic column system comprises the following steps:

[0183] Step 1, equilibration of the first chromatographic column 5-1 and the second chromatographic column 5-2

[0184] The second chromatographic pump 4-2 and the third chromatographic pump 4-3 are started, the pre-column valve 10 is switched to connect the first chromatographic column 5-1, the post-column valve 6 is switched to connect the first chromatographic column 5-1 and the waste tank 9, and the chromatographic pump is operated according to the time program and the proportion of the mobile phase to perform pre-injection equilibration of the first chromatographic column 5-1; after the above steps are completed, the second chromatographic pump 4-2 and the third chromatographic pump 4-3 are started, the pre-column valve 10 is switched to connect the second chromatographic column 5-2, the post-column valve 6 is switched to connect the second chromatographic column 5-2 and the waste tank 9, and the chromatographic pump is operated according to the time program and the proportion of the mobile phase to perform pre-injection equilibration of the second chromatographic column 5-2.

[0185] Step 2, sample injection of the first chromatographic column 5-1:

[0186] Start the first chromatographic pump 4-1, switch the pre-column valve 10 to connect the first chromatographic column 5-1, switch the post-column valve 6 to connect the first chromatographic column 5-1 with the waste tank 9, and run the chromatographic pump according to the time program to inject sample into the first chromatographic column.

[0187] Step 3, elution-column washing-equilibrium of the first chromatographic column 5-1, sample injection of the second chromatographic column 5-2:

[0188] Start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve 10 to connect the first chromatographic column 5-1, and at the same time, the first chromatographic pump connects the second chromatographic column 5-2. Switch the post-column valve 6 to connect the first chromatographic column 5-1 with the detector device 7, and at the same time, the second chromatographic column 5-2 is connected with the waste tank 9. Run the second chromatographic pump 4-2 and the third chromatographic pump 4-3 according to the time program and the proportion of the mobile phase to elute, wash, and balance the first chromatographic column 5-1, and at the same time, run the first chromatographic pump to inject sample into the second chromatographic column 5-2.

[0189] Step 4, sample injection of the first chromatographic column 5-1, elution-column washing-equilibrium of the second chromatographic column 5-2:

[0190] Start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve to connect the two chromatographic pumps with the second chromatographic column 5-2, and at the same time, the first chromatographic pump connects the first chromatographic column 5-1. Switch the post-column valve 6 to connect the second chromatographic column 5-2 with the detector device 7, and at the same time, the first chromatographic column 5-1 is connected with the waste tank 9. Run the second chromatographic pump 4-2 and the third chromatographic pump 4-3 according to the time program and the proportion of the mobile phase to elute, wash, and balance the second chromatographic column 5-2, and at the same time, run the first chromatographic pump to inject sample into the first chromatographic column 5-1.

[0191] Step 5, cycle: according to the above two steps, cycle the chromatographic column balance-sample-elution-column washing of the two chromatographic columns to collect the sample.

Claims

1. An automated chromatography system with multiple parallel columns, comprising a sample container (1), a mobile phase device (2), a multi-port solvent selection valve (3), a chromatography pump (4), a chromatography column device (5), a post-column valve (6), a detector device (7), a fraction collector (8), a waste liquid tank (9), and connecting pipes; characterized in that, The mobile phase device includes at least two mobile phase storage tanks connected in parallel. The number of chromatography pumps is greater than or equal to 2, and they are set in parallel. The chromatographic column apparatus includes at least two identical chromatographic columns arranged in parallel. The detector device includes at least one detector. The detector device is installed in parallel with the waste liquid tank (9); Among them, the sample container (1) and the mobile phase device (2) are connected in parallel and then connected to the multi-port solvent selection valve (3) through a connecting pipe. The multi-port solvent selection valve (3) is connected to the chromatography pump (4) through a connecting pipe. The chromatography pump (4) is connected to the chromatography column device (5) through a connecting pipe. The chromatography column device (5) is connected to the post-column valve (6) through a connecting pipe. The post-column valve (6) is connected to the detector device (7) and the waste liquid tank (9) in parallel through a connecting pipe. The detector device (7) is connected to the fraction collector (8) through a connecting pipe.

2. An automated chromatography system with multiple parallel columns, comprising a sample container (1), a mobile phase device (2), a chromatography pump (4), a chromatography column device (5), a post-column valve (6), a detector device (7), a fraction collector (8), a waste liquid tank (9), a pre-column valve (10), and connecting pipes; Its features are, The mobile phase device includes at least two mobile phase storage tanks connected in parallel. The number of chromatography pumps is greater than or equal to 3. The chromatographic column apparatus includes at least two identical chromatographic columns arranged in parallel. The detector device includes at least one detector. The detector device is connected in parallel with the waste liquid tank; Among them, the sample container (1) is connected to the chromatography pump (4) through a connecting pipe to form a sample delivery path. The mobile phase storage tanks set in parallel are connected to the chromatography pump (4) through connecting pipes and then connected to form a mobile phase delivery path through connecting pipes. The sample delivery path and the mobile phase delivery path are connected to the column valve (10) through connecting pipes. The column valve (10) is connected to the chromatography column device (5) through connecting pipes. The chromatography column device (5) is connected to the column valve (6) through connecting pipes. The column valve (6) is connected to the detector device (7) and the waste liquid tank (9) in parallel through connecting pipes. The detector device (7) is connected to the fraction collector (8) through connecting pipes.

3. The automated chromatography system according to claim 1, characterized in that, It also includes a column pre-valve (10), which is located before the chromatographic pump or the chromatographic column.

4. The automated chromatography system according to any one of claims 1 and 2, characterized in that, When the detector device includes two or more detectors, the detectors are identical and connected in parallel.

5. The automated chromatography system according to any one of claims 1 and 2, characterized in that, The chromatographic column is selected from normal phase chromatographic columns, reversed phase chromatographic columns, ion exchange columns, and gel chromatographic columns.

6. The automated chromatography system according to any one of claims 1 and 2, characterized in that, The detectors are selected from ultraviolet detectors, conductivity detectors, diode array detectors, evaporative light scattering detectors, and mass spectrometry detectors.

7. The automated chromatography system according to any one of claims 1 and 2, characterized in that, In gradient mode, the chromatography pump is selected from one or both of the following: a conventional pump and a high-precision pump.

8. The automated chromatography system according to claim 1, characterized in that, In gradient mode, the automated chromatography system also includes a proportional valve (11).

9. The automated chromatography system according to claim 8, characterized in that, In gradient mode, gradient elution can be controlled by a proportional valve (11).

10. The automated chromatography system according to claim 2, characterized in that, In gradient mode, gradient elution can be achieved by controlling the chromatographic pump (4).

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