Automatic dyeing mechanism of biological tissue sample section dyeing equipment

Abstract

The utility model provides an automatic dyeing mechanism of biological tissue sample section dyeing equipment, which comprises an automatic dyeing glass slide feeding and clamping component, an automatic dyeing reaction disc and an automatic dyeing glass slide discharging and clamping component, a main console of the biological tissue sample section dyeing equipment is in control connection with an automatic dyeing glass slide feeding and clamping assembly, an automatic dyeing reaction disc and an automatic dyeing glass slide discharging and clamping assembly, the automatic dyeing reaction disc is provided with a plurality of dyeing grooves, and the dyeing grooves can be filled with dyeing reagents and are matched with microscope glass slides. The automatic dyeing glass slide feeding and clamping assembly and the automatic dyeing glass slide discharging and clamping assembly are both matched with the feeding and discharging temporary storage mechanism, and the feeding and discharging temporary storage mechanism can place a plurality of microscope glass slide vertical placing supports in an array mode. The microscope glass slide dyeing tank has the beneficial effects that the microscope glass slides can be separated by the dyeing tank for dyeing operation, so that cross contamination is avoided.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue sample staining technology, specifically to an automatic staining mechanism for a biological tissue sample staining device. Background Technology

[0002] In existing biological sample analysis techniques, commonly used analytical methods include microscopy, microarray analysis (e.g., protein and nucleic acid microarray analysis), and mass spectrometry. Preparing samples for these and other types of analysis typically involves contacting the biological sample with a series of processing liquids. Some of these processing liquids (e.g., staining reagents and counterstaining reagents) can add color and, conversely, alter the visual properties of invisible or difficult-to-see sample components (e.g., at least some types of cells and intracellular structures). Other processing liquids (e.g., deparaffin liquids) can be used to achieve other processing purposes. If multiple processing liquids are used to treat the sample, the application and subsequent removal of each liquid can be crucial for producing a sample suitable for analysis. In some cases, treating samples with multiple processing liquids involves manually applying the liquids to the microscope slides holding the samples. However, this manual sample preparation method tends to be labor-intensive and inaccurate, leading to the development of biological tissue section staining equipment.

[0003] Automated staining technology for biological tissue samples is a crucial and indispensable step in the entire automated staining, drying, and mounting process for biological tissue samples. To ensure that the processed biological tissue samples have an easily observable appearance and maintain consistency and efficiency in the processing, the staining step is an essential aspect that must be strictly controlled during biological tissue sample processing. The purpose of staining is to make different structures within the cellular tissue appear in different colors for easier observation. The classic hematoxylin and eosin staining method is the routine staining method for histological and pathological specimens, abbreviated as HE staining. After staining, the cell nuclei are stained purple-blue by hematoxylin, while most cytoplasm and non-cellular components are stained pink by eosin.

[0004] Existing biological tissue slide staining equipment can replace manual staining. These machines automate sample processing by immersing racks carrying batches of microscope slides in an open bath of processing liquid. However, existing biological tissue slide staining equipment places batches of microscope slides containing biological tissue sample sections horizontally in slide holders. Although there is some vertical space between each microscope slide, the entire box of slides is processed together during the staining operation. When the spacers are immersed in the shared staining reagent, cross-contamination of the microscope slides within the same box is inevitable. For example, cells from a biological tissue sample section can leave the sample on one microscope slide and move to another microscope slide in the shared staining reagent. This form of contamination significantly reduces the accuracy of certain types of sample analysis. Furthermore, when a box of microscope slides is placed horizontally, the osmotic pressure of the staining reagent on the upper microscope slide is lower than that on the lower microscope slide after immersion in the shared staining reagent. This inevitably leads to uneven staining between the upper and lower microscope slides, resulting in poor consistency in the quality of the final stained biological tissue sample slides and a very poor user experience. Utility Model Content

[0005] To address the problems in existing technologies, this utility model provides an automatic staining mechanism for a biological tissue sample section staining device. This mechanism comprises a cooperating automatic staining slide feeding and gripping assembly, an automatic staining reaction disk, and an automatic staining slide discharging and gripping assembly. The staining tank of the automatic staining reaction disk can separate each microscope slide for staining, avoiding the risk of cross-contamination between microscope slides containing biological tissue samples. This improves the user experience and solves the problems of cross-contamination risk and poor product consistency in existing biological tissue sample section staining devices.

[0006] This utility model provides an automatic staining mechanism for a biological tissue sample section staining device, which cooperates with the loading and unloading buffer mechanism of the biological tissue sample section staining device. It includes an automatic staining slide feeding and gripping assembly, an automatic staining reaction tray, and an automatic staining slide discharging and gripping assembly. The automatic staining slide feeding and gripping assembly, the automatic staining reaction tray, and the automatic staining slide discharging and gripping assembly are all fixedly mounted on the worktable. The main control console of the biological tissue sample section staining device is controllably connected to the automatic staining slide feeding and gripping assembly, the automatic staining reaction tray, and the automatic staining slide discharging and gripping assembly. The automatic staining reaction tray is equipped with multiple components for individual display... A staining tank for microscopy slide staining is provided, which can be filled with staining reagent and is compatible with microscope slides. The automatic staining slide feeding and discharging components are both coordinated with the loading and unloading buffer mechanism. The loading and unloading buffer mechanism can array multiple microscope slide vertical supports. The automatic staining slide feeding and discharging components can pick up microscope slides from the vertical supports on the loading and unloading buffer mechanism and move them into the staining tank for staining. After the microscope slides have been stained and returned to the vertical supports, they are discharged by the loading and unloading buffer mechanism.

[0007] In a further improvement of this utility model, the automatic staining reaction disk is provided with a reaction disk rotating shaft in the middle, and the automatic staining reaction disk is fixedly connected to the top of the reaction disk rotating shaft.

[0008] In a further improvement of this utility model, a reaction disk rotation drive motor is provided at the bottom of the reaction disk shaft. The reaction disk rotation drive motor is fixedly mounted on the worktable and is connected to the reaction disk shaft for rotation drive.

[0009] This utility model is further improved in that the automatic staining slide feeding and gripping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven connected to the bottom of the automatic staining slide feeding shaft. The top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction disk.

[0010] This utility model is further improved in that the automatic staining slide feeding and gripping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven connected to the bottom of the automatic staining slide feeding shaft. The top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction disk.

[0011] The present invention is further improved by including a reagent adding mechanism, which cooperates with the automatic staining reaction plate. The main control console of the biological tissue sample section staining equipment is controlled and connected to the reagent adding mechanism, which is capable of adding staining reagent into the staining tank.

[0012] The present invention is further improved by including a staining tank cleaning component, which cooperates with the automatic staining reaction tray. The main control console of the biological tissue sample slide staining device is connected to the staining tank cleaning component for control. The staining tank cleaning component can extract the staining reagent in the staining tank.

[0013] The present invention is further improved by including a slide unloading and cleaning assembly, which works in conjunction with the automatic staining slide unloading robot, and the slide unloading and cleaning assembly is capable of cleaning microscope slides that have completed staining operations.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows: This utility model provides an automatic staining mechanism for a biological tissue sample section staining device. By setting up an automatically stained slide feeding and gripping assembly, an automatic staining reaction tray, and an automatically stained slide discharging and gripping assembly within the automatic staining mechanism, the automatic stained slide feeding and gripping assembly can grip microscope slides from the microscope slide vertical support on the loading and unloading buffer mechanism, move them into the staining tank for staining, and after the microscope slides have completed the staining process and are returned to the microscope slide vertical support, they are then transported back to the loading and unloading buffer mechanism. After the material is discharged, the staining tank of the automatic staining reaction tray can separate each microscope slide for staining, avoiding the risk of cross-contamination between microscope slides containing biological tissue sample sections. Moreover, each microscope slide is immersed in the shared staining reagent and experiences the same osmotic pressure, resulting in better consistency in the quality of the stained biological tissue sample microscope slides. This improves the quality of biological tissue sample microscope slides, enhances the user experience, and solves the problems of cross-contamination risk and poor product consistency in existing biological tissue sample staining equipment. Attached Figure Description

[0015] Figure 1 This is a structural diagram of the automatic staining mechanism of the biological tissue sample section staining device of this utility model.

[0016] In the figure, 2-loading and unloading buffer mechanism, 3-automatic staining mechanism, 31-automatic staining slide feeding and gripping assembly, 32-automatic staining reaction tray, 321-staining tank, 33-automatic staining slide discharge and gripping assembly, 4-reagent adding mechanism, 35-staining tank cleaning assembly, and 36-slide discharge and cleaning assembly. Detailed Implementation

[0017] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0018] like Figure 1As shown, the automatic staining mechanism 3 of the biological tissue sample section staining device provided by this utility model cooperates with the loading and unloading buffer mechanism 2 of the biological tissue sample section staining device. It includes an automatic staining slide feeding and gripping assembly 31, an automatic staining reaction tray 32, and an automatic staining slide discharging and gripping assembly 33. The automatic staining slide feeding and gripping assembly 31, the automatic staining reaction tray 32, and the automatic staining slide discharging and gripping assembly 33 are all fixedly mounted on the worktable. The main control console of the biological tissue sample section staining device is controlled and connected to the automatic staining slide feeding and gripping assembly 31, the automatic staining reaction tray 32, and the automatic staining slide discharging and gripping assembly 33. The 32 is equipped with multiple staining tanks 321 for staining individual microscope slides. The staining tanks 321 can be filled with staining reagents and are compatible with microscope slides. The automatic staining slide feeding and clamping assembly 31 and the automatic staining slide discharging and clamping assembly 32 are both coordinated with the loading and unloading buffer mechanism 2. The loading and unloading buffer mechanism 2 can arrange multiple microscope slide vertical supports in an array. The automatic staining reaction disk 32 has a reaction disk rotating shaft in the middle. The automatic staining reaction disk 32 is fixedly connected to the top of the reaction disk rotating shaft. The bottom of the reaction disk rotating shaft is equipped with a reaction disk rotation drive motor. The reaction disk rotation drive motor is fixedly mounted on the worktable and is rotated and driven by the reaction disk rotating shaft. In this embodiment, the automatic staining slide feeding and gripping component can grip the microscope slides in the microscope slide vertical support on the loading and unloading buffer mechanism, move them into the staining tank for staining, and after the microscope slides have completed the staining process, they are returned to the microscope slide vertical support and then discharged through the loading and unloading buffer mechanism. The staining tank of the automatic staining reaction disk can separate each microscope slide for staining, avoiding the risk of cross-contamination between microscope slides containing biological tissue sample sections. Moreover, each microscope slide is immersed in the shared staining reagent and experiences the same osmotic pressure of the staining reagent, resulting in good consistency in the quality of the stained biological tissue sample section microscope slides. This improves the product quality of the biological tissue sample section microscope slides and enhances the user experience.

[0019] like Figure 1As shown, the automatic staining slide feeding and gripping assembly 31 includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven to the bottom of the automatic staining slide feeding shaft, and the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading / unloading buffer mechanism 2 and the automatic staining reaction plate 32. In this embodiment, driven by the automatic staining slide feeding motor, the automatic staining slide feeding robot can grip microscope slides in the microscope slide vertical support on the loading / unloading buffer mechanism and move them into the staining tank for staining.

[0020] like Figure 1 As shown, the automatic staining slide feeding and gripping assembly 33 includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven to the bottom of the automatic staining slide feeding shaft, and the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading / unloading buffer mechanism 2 and the automatic staining reaction disk 32. In this embodiment, the automatic staining slide feeding robot, driven by the automatic staining slide feeding motor, can grip the microscope slides that have completed staining in the staining tank and move them into the microscope slide vertical support on the loading / unloading buffer mechanism.

[0021] like Figure 1 As shown, it also includes a reagent adding mechanism 4, which cooperates with the automatic staining reaction tray 32. The main control console of the biological tissue sample section staining equipment is connected to the reagent adding mechanism 4, and the reagent adding mechanism 4 can add staining reagent into the staining tank 321. In this embodiment, the reagent adding mechanism can add staining reagent into the staining tank according to the control instructions of the main control console of the biological tissue sample section staining equipment.

[0022] like Figure 1 As shown, the system also includes a staining tank cleaning assembly 35, which works in conjunction with the automatic staining reaction tray 32. The main control console of the biological tissue sample section staining equipment is connected to the staining tank cleaning assembly 35, which is capable of extracting staining reagents from the staining tank 321. In this embodiment, the staining tank cleaning assembly can extract staining reagents from the staining tank according to the control commands from the main control console of the biological tissue sample section staining equipment.

[0023] like Figure 1As shown, the system also includes a slide unloading and cleaning assembly 36, which works in conjunction with an automated staining slide unloading robot. The slide unloading and cleaning assembly 36 cleans microscope slides that have completed staining. In this embodiment, the slide unloading and cleaning assembly contains cleaning reagents. Microscope slides that have completed staining can be cleaned by the slide unloading and cleaning assembly before being unloaded. During the staining process, each microscope slide can be placed into the slide unloading and cleaning assembly for cleaning after each staining operation in the staining tank, and then placed into the next staining tank for staining according to the staining procedure.

[0024] As can be seen from the above, the automatic staining mechanism of the biological tissue sample section staining device provided by this utility model, by setting up an automatic staining slide feeding and gripping component, an automatic staining reaction tray, and an automatic staining slide discharging and gripping component that cooperate with each other, allows the automatic staining slide feeding and gripping component to grip microscope slides in the microscope slide vertical support on the loading and unloading buffer mechanism, move them into the staining tank for staining, and after the microscope slide has completed the staining operation and is put back into the microscope slide vertical support, it is discharged by the loading and unloading buffer mechanism, thus automatically staining the slides. The staining tank of the color reaction disk can separate each microscope slide for staining, avoiding the risk of cross-contamination between microscope slides containing biological tissue sample sections. Moreover, each microscope slide is immersed in the shared staining reagent and experiences the same osmotic pressure, resulting in better consistency in the quality of the stained biological tissue sample microscope slides. This improves the quality of biological tissue sample microscope slides, enhances the user experience, and solves the problems of cross-contamination risk and poor product consistency in existing biological tissue sample staining equipment.

[0025] The specific embodiments described above are preferred embodiments of this utility model, and are not intended to limit the specific scope of this utility model. The scope of this utility model includes but is not limited to the specific embodiments described above. All equivalent changes made in accordance with this utility model are within the protection scope of this utility model.

Claims

1. An automatic staining mechanism for a biological tissue sample section staining device, which cooperates with the loading and unloading buffer mechanism of the biological tissue sample section staining device, characterized in that: The system includes an automated slide feeding and gripping assembly, an automated staining reaction tray, and an automated slide dispensing and gripping assembly. These three assemblies are all fixedly mounted on the worktable. The main control console of the biological tissue sample section staining equipment is connected to these assemblies. The automated staining reaction tray has multiple staining tanks for staining individual microscope slides, and each tank can be filled with staining reagents. The staining agent is compatible with microscope slides. The automatic staining slide feeding and discharging components are both coordinated with the loading and unloading buffer mechanism. The loading and unloading buffer mechanism can array multiple microscope slide vertical supports. The automatic staining slide feeding and discharging components can clamp microscope slides from the microscope slide vertical supports on the loading and unloading buffer mechanism and move them into the staining tank for staining. After the microscope slides have completed the staining process and are returned to the microscope slide vertical supports, they are discharged through the loading and unloading buffer mechanism.

2. The automatic staining mechanism of the biological tissue sample section staining device according to claim 1, characterized in that: The automatic staining reaction disk has a reaction disk shaft in the middle, and the automatic staining reaction disk is fixedly connected to the top of the reaction disk shaft.

3. The automatic staining mechanism of the biological tissue sample section staining device according to claim 2, characterized in that: The bottom of the reaction disk shaft is equipped with a reaction disk rotation drive motor, which is fixedly mounted on the worktable and is connected to the reaction disk shaft for rotation drive.

4. The automatic staining mechanism of the biological tissue sample section staining device according to claim 3, characterized in that: The automatic staining slide feeding and gripping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven connected to the bottom of the automatic staining slide feeding shaft. The top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction disk.

5. The automatic staining mechanism of the biological tissue sample section staining device according to claim 4, characterized in that: The automatic staining slide feeding and gripping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft, and an automatic staining slide feeding robot. The automatic staining slide feeding motor is fixedly mounted on the worktable. The automatic staining slide feeding motor is rotatably driven connected to the bottom of the automatic staining slide feeding shaft. The top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding robot. The automatic staining slide feeding robot can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction disk.

6. The automatic staining mechanism of the biological tissue sample section staining device according to claim 5, characterized in that: It also includes a reagent adding mechanism, which works in conjunction with the automatic staining reaction tray. The main control console of the biological tissue sample section staining equipment is connected to the reagent adding mechanism, which is capable of adding staining reagents into the staining tank.

7. The automatic staining mechanism of the biological tissue sample section staining device according to claim 6, characterized in that: It also includes a staining tank cleaning assembly, which works in conjunction with the automatic staining reaction tray. The main control console of the biological tissue sample slide staining equipment is connected to the staining tank cleaning assembly, which is capable of extracting staining reagents from the staining tank.

8. The automatic staining mechanism of the biological tissue sample section staining device according to claim 7, characterized in that: It also includes a slide unloading and cleaning assembly, which works in conjunction with the automatic staining slide unloading robot, and the slide unloading and cleaning assembly can clean microscope slides that have completed staining operations.

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