Cryopreservation tube capable of directly carrying out flow type detection

Abstract

The utility model relates to the technical field of flow type detection, and discloses a cryopreservation tube capable of directly carrying out flow type detection, which comprises an outer cavity, an inner cavity is arranged in the outer cavity, a protective agent layer is arranged between the outer cavity and the inner cavity, a threaded connection part is arranged on the upper side of the outer cavity, and the threaded connection part is connected with the inner cavity. And a sealing cover corresponding to the threaded connection part is arranged on the upper side of the outer cavity. By adopting the integrated double-cavity structure, the whole cryopreservation tube can be subjected to cryopreservation operation before flow type detection, when the cryopreservation tube is used, the outer cavity can be directly removed, the inner cavity is directly inserted into a flow type instrument, pipetting is not needed, the whole cryopreservation tube is convenient to use, and the protective agent layer is arranged, so that after a sample is injected into the inner cavity, the cryopreservation tube is convenient to use. When the cell sample cryopreservation device is used, an operator presses the pressing movable cover, the protective agent layer can be broken, so that the protective agent is discharged, the cell sample can be protected in the cryopreservation process, and the cryopreservation effect of the cell sample is improved.

Description

Technical Field

[0001] This utility model relates to the field of flow cytometry technology, specifically a cryopreservation tube that can be directly used for flow cytometry. Background Technology

[0002] Flow cytometry is a technique for rapid quantitative analysis and sorting of single cells or other biological particles in a liquid stream.

[0003] In current practice, samples are generally aliquoted and frozen using cryopreservation tubes or 1.5 / 2 mL centrifuge tubes. However, during the aliquoting and freezing process, the samples need to be transferred from the 1.5 mL centrifuge tubes to flow cytometry tubes, which increases the pipetting steps. Repeated freeze-thaw cycles can lead to data deviations. In addition, multiple opening operations can easily introduce microbial or aerosol contamination, and residual liquid during the transfer process can cause cell / protein loss. Utility Model Content

[0004] To address the aforementioned technical problems, this invention provides a cryopreservation tube that can be directly subjected to flow cytometry analysis, offering advantages such as ease of operation and excellent cryopreservation results.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A cryopreservation tube that can be directly analyzed by flow cytometry includes an outer cavity, an inner cavity inside the outer cavity, a protective agent layer between the outer cavity and the inner cavity, a threaded connection part on the upper side of the outer cavity, and a sealing cap corresponding to the threaded connection part on the upper side of the outer cavity.

[0007] The inner wall of the lower side of the sealing cap is provided with an internal thread corresponding to the threaded connection part. The middle part of the upper side of the sealing cap is provided with an opening. Guide grooves are provided on both sides of the inner wall of the opening. A pressure block is slidably connected to the guide groove. A movable cover is provided on the upper side of the pressure block. A puncture membrane is provided on the lower side of the pressure block. A puncture part is provided on the lower side of the protective agent layer. The upper side of the puncture part is designed as an arc that matches the lower side of the inner cavity.

[0008] In the above scheme: a pull ring is provided on the upper side of the movable cover.

[0009] In the above scheme: a first magnetic block is provided on both sides of the upper side of the pressure block, and a corresponding second magnetic block is provided on both sides of the lower side of the movable cover.

[0010] In the above scheme: the distance between the two pressure blocks is slightly smaller than the outer diameter of the upper side of the inner cavity, and the inner cavity is provided with a scale.

[0011] In the above scheme: a sealing ring is provided on the lower side of the sealing cover.

[0012] In the above scheme: a temperature sensing tag is provided on the outer side of the outer cavity.

[0013] Beneficial effects

[0014] This invention provides a cryopreservation tube that can be directly analyzed by flow cytometry, and has the following advantages:

[0015] 1. This technical solution adopts an integrated dual-chamber structure, which allows the cryopreservation tube to be frozen as a whole before flow cytometry. When in use, the outer chamber can be removed directly and the inner chamber can be directly inserted into the flow cytometer without the need for pipetting, making it convenient to use as a whole.

[0016] 2. By setting a protective layer, after the sample is injected into the inner cavity, the operator can press down the movable cap to break the protective layer, thereby expelling the protective agent. This can enhance the protection of cell samples during cryopreservation and improve the cryopreservation effect of cell samples.

[0017] 3. By setting a sealing cap and cooperating with the movable cap and the puncture membrane, the sealing between the inner and outer cavities can be ensured, which will not affect the puncture of the flow cytometer injection needle and reduce the possibility of contamination when the cap is opened. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the front sectional view of the present invention.

[0019] Figure 2 This is a schematic diagram of the cross-sectional structure of the outer cavity of this utility model.

[0020] Figure 3 This is a schematic diagram of the sealing cap assembly state of this utility model.

[0021] Figure 4 This is a top view of the external cavity structure of this utility model.

[0022] Figure 5 This is a partially enlarged structural schematic diagram of the present invention.

[0023] In the diagram: 1. Outer cavity, 2. Inner cavity, 3. Protective layer, 4. Threaded connection, 5. Sealing cap, 6. Pressure block, 7. Movable cap, 8. Puncture membrane, 9. Puncture part, 10. Pull ring, 11. First magnetic block, 12. Second magnetic block, 13. Sealing ring, 14. Temperature sensing tag. Detailed Implementation

[0024] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0025] Example

[0026] Please see Figure 1-5 A cryopreservation tube that can be directly flow cytometry tested includes an outer cavity 1, an inner cavity 2 inside the outer cavity 1, a protective agent layer 3 between the outer cavity 1 and the inner cavity 2, a threaded connection part 4 on the upper side of the outer cavity 1, and a sealing cap 5 corresponding to the threaded connection part 4 on the upper side of the outer cavity 1.

[0027] The inner wall of the lower side of the sealing cap 5 is provided with an internal thread corresponding to the threaded connection part 4. The middle part of the upper side of the sealing cap 5 is provided with an opening. Guide grooves are provided on both sides of the inner wall of the opening. The guide grooves are slidably connected to the pressure block 6. The upper side of the pressure block 6 is provided with a movable cover 7. The lower side of the pressure block 6 is provided with a puncture membrane 8. The lower side of the protective agent layer 3 is provided with a puncture part 9. The upper side of the puncture part 9 is set with an arc shape that matches the lower side of the inner cavity 2.

[0028] It should be noted that in this technical solution, the inner cavity 2 is of flow cytometry specification, that is, the standard size of 12×75 mm, which is compatible with flow cytometers / centrifuges / PCR instruments. Meanwhile, the outer cavity 1 is compatible with standard cryopreservation racks. The outer layer of the cryoprotectant layer 3 is made of polyethylene and is filled with cell sedimentation stabilizer and cryoprotectant.

[0029] Before use, the operator first assembles the outer cavity 1 and the inner cavity 2, and with the help of the threaded connection part 4, the sealing cover 5 can be connected to the outer cavity 1 and the outer cavity 1 and the inner cavity 2 can be sealed. When it is necessary to use the flow cytometer to inject samples into the inner cavity 2, the movable cover 7 is first removed with the pull ring 10 to expose the puncture membrane 8. Finally, the injection needle can be used to puncture the puncture membrane 8 and inject samples into the inner cavity 2. After the injection operation is completed, the movable cover 7 can be connected to the pressure block 6 again with the first magnetic block 11 and the second magnetic block 12 to ensure the overall sealing performance of the sealing cover 5.

[0030] The operator then presses down the movable cover 7 and the inner cavity 2 through the pressure block 6, causing the inner cavity 2 to move slightly downward and drive the puncture part 9 to puncture the outer layer of the cryoprotectant layer 3, allowing the cell sedimentation stabilizer and cryoprotectant inside the cryoprotectant layer 3 to be discharged. Because cells are prone to sedimentation and mutual compression during cryopreservation due to gravity and other factors, the cryopreservation effect of cells is affected. The cell sedimentation stabilizer can prevent cells from sedimenting too quickly and prevent cells from compressing each other. At the same time, the cryoprotectant can prevent the formation of ice crystals inside the cells, maintain the osmotic pressure balance inside and outside the cells, and stabilize intracellular proteins and biomolecules. The combination of the two can increase the cryopreservation effect of cell samples and ensure the quality of cryopreservation.

[0031] After the protective layer 3 ruptures and the cell sedimentation stabilizer and cryoprotectant are expelled, the outer cavity 1 can be placed inside the cryopreservation rack for cryopreservation. When flow cytometry is required, the sealing cap 5 can be removed, the inner cavity 2 can be taken out, and the inner cavity 2 can be directly inserted into the flow cytometer without the need for pipetting. The overall operation is simple.

[0032] The movable cover 7 is provided with a pull ring 10 on the upper side, and the pressure block 6 is provided with first magnetic blocks 11 on both sides of the upper side. The movable cover 7 is provided with corresponding second magnetic blocks 12 on both sides of the lower side. The movable cover 7 can be removed as a whole, which facilitates flexible sample injection and pressing operations in the inner cavity 2, and facilitates pressing down the inner cavity 2 and puncturing the protective agent layer 3 with the puncture part 9.

[0033] The distance between the two pressure blocks 6 is slightly smaller than the outer diameter of the upper side of the inner cavity 2, which makes it easier to press down the inner cavity 2. The inner cavity 2 is provided with a scale on the front side, with the scale being 50 / 100 / 200μL.

[0034] A sealing ring 13 is provided on the lower side of the sealing cover 5 to increase the overall sealing performance of the sealing cover 5.

[0035] A temperature sensing tag 14 is provided on the outer side of the outer cavity 1. The temperature sensing tag 14 is mainly made of cholesterol material, and the specific color change temperature range is between -50 and 200℃, which facilitates real-time display of the freezing temperature and prevents abnormal freezing temperature.

[0036] This technical solution adopts an integrated dual-chamber structure and is equipped with a protective agent layer 3. The overall operation is simple and convenient, reducing the number of pipetting steps. At the same time, the sample cryopreservation effect is good. With the sealing cap 5, the wall can be opened multiple times, which can easily introduce microorganisms or aerosol contamination, and the sample loss can be reduced.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cryotube that can be directly subjected to flow detection, comprising an outer chamber (1), characterized in that, The outer cavity (1) is internally provided with an inner cavity (2), a protective agent layer (3) is arranged between the outer cavity (1) and the inner cavity (2), a threaded connection part (4) is arranged on the upper side of the outer cavity (1), and a sealing cover (5) corresponding to the threaded connection part (4) is arranged on the upper side of the outer cavity (1); Corresponding internal threads are formed in the inner wall of the lower side of the sealing cover (5), an opening is arranged in the middle part of the upper side of the sealing cover (5), guide grooves are formed in the inner walls on both sides of the opening, a pressing block (6) is slidably connected to the guide grooves, a movable cover (7) is arranged on the upper side of the pressing block (6), a puncture film (8) is arranged on the lower side of the pressing block (6), a puncture part (9) is arranged on the lower side of the protective agent layer (3), and the upper side of the puncture part (9) is arranged in an arc shape matching the lower side of the inner cavity (2).

2. The cryo tube capable of direct flow detection according to claim 1, characterized in that, The upper side of the movable cover (7) is provided with a pull ring (10).

3. The cryo tube capable of direct flow detection according to claim 2, characterized in that, First magnetic attraction blocks (11) are arranged on both sides of the upper side of the pressing block (6), and corresponding second magnetic attraction blocks (12) are arranged on both sides of the lower side of the movable cover (7).

4. The cryo tube capable of direct flow detection according to claim 3, characterized in that, The distance between the two pressing blocks (6) is slightly smaller than the outer diameter of the upper side of the inner cavity (2), and a scale is arranged on the front side of the inner cavity (2).

5. The cryo tube capable of direct flow detection according to claim 4, characterized in that, A sealing ring (13) is arranged on the lower side of the sealing cover (5).

6. The cryo tube capable of direct flow detection according to claim 5, characterized in that, A temperature sensing label (14) is arranged on the outer side of the outer cavity (1).

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