Tissue culture bottle for tissue culture seedlings
By introducing a ventilating mechanism into the tissue culture flask, the problem of impurity intrusion caused by the ventilating tube was solved, and the ventilating effect was made controllable and the tissue culture effect was improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- 济南格艾特仪器设备有限公司
- Filing Date
- 2025-07-08
- Publication Date
- 2026-04-21
AI Technical Summary
Existing tissue culture bottles for tissue culture seedlings have simple vent tube designs, which easily allow dust and other impurities to enter, affecting the plant tissue culture effect.
A tissue culture bottle with a venting mechanism was designed, including a venting tube, a fixing plate, a sealing block, an end rod, a guide rod, and a spring structure. Through the coordinated use of these components, controllable venting of the venting tube can be achieved, preventing the intrusion of impurities.
It achieves controllable air permeability, avoids the intrusion of dust and other impurities, and improves the diversity and effectiveness of tissue culture plants.
Smart Images

Figure CN224139819U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of tissue culture bottle technology, specifically a tissue culture bottle for tissue culture seedlings. Background Technology
[0002] As is well known, tissue culture bottles are specialized containers used in plant tissue culture to culture plant cells, tissues, or organs. They play an important role in plant propagation, genetic improvement, and germplasm preservation.
[0003] A utility model patent with patent authorization announcement number CN213153361U discloses a tissue culture bottle for tissue culture seedlings, including a bottle body, a bottle base provided on the lower outer surface of the bottle body, a grid room provided inside the bottle body, the grid room being located at the upper end of the bottle base, and a venting tube provided on the upper outer surface of the bottle body.
[0004] However, existing tissue culture bottles for tissue culture seedlings also have certain shortcomings. Most existing tissue culture bottles for tissue culture seedlings simply use a combination of bottle cap and bottle body to complete the tissue culture and observation treatment of plants. Due to the influence of plant respiration and other production processes, simply using a ventilator for ventilation will cause the intrusion of dust and other impurities. Utility Model Content
[0005] The purpose of this utility model is to provide a tissue culture bottle for tissue culture seedlings, which solves the problem that existing tissue culture bottles for tissue culture seedlings mostly use a simple combination of bottle cap and bottle body to complete the tissue culture and observation treatment of plants. Due to the influence of plant respiration and other production processes, simply using a ventilator for ventilation will cause dust and other impurities to enter.
[0006] To achieve the above objectives, this utility model provides the following technical solution: a tissue culture bottle for tissue culture seedlings, comprising a bottle body, a bottle cap connected to the inner wall of the bottle body by a thread, a venting tube fixedly connected to the inner wall of the bottle cap, the vertical part of the venting tube being located inside the bottle body, a venting mechanism being provided on the horizontal part of the venting tube, an installation cylinder contacting the bottom inner side of the bottle body, a partition plate fixedly connected to the inner wall of the installation cylinder, a slot being provided on the side of the installation cylinder, a locking plate being slidably connected to the inner wall of the slot, a spring being welded to the end face of the locking plate away from the partition plate, and the other end of the spring being fixedly connected to the bottle body.
[0007] Preferably, there are two slots, which are symmetrically distributed on the mounting cylinder. The slots facilitate insertion and use with the card plate.
[0008] Preferably, the surface of the card plate is fixedly connected with protrusions, which are made of rubber. The protrusions facilitate the movement of the card plate, and the rubber material has good durability.
[0009] Preferably, the ventilation mechanism includes a fixed plate, the fixed plate is fixedly connected to the horizontal part of the ventilation tube, a sealing block is slidably connected to the inner wall of the fixed plate, an end rod is fixedly connected to the right end of the sealing block, an end block is fixedly connected to the inner wall of the ventilation tube, a guide rod is fixedly connected to the side of the end rod, the guide rod is slidably connected to the ventilation tube, and a second spring is provided on the outer side of the end rod. Through the cooperation of the end rod, the second spring, and other structures, the sealing block can be disengaged from the fixed plate, thereby ensuring the ventilation effect of the ventilation tube.
[0010] Preferably, an end ball is fixedly connected to the right end of the end rod. The end ball is made of silicone. The end ball facilitates the movement of the end rod by pulling it, and the silicone material has a good skin-friendly feel.
[0011] Preferably, two guide rods are provided, which are symmetrically distributed on the end rod. The end rod can be moved and guided by the guide rods.
[0012] Preferably, one end of the second spring is fixedly connected to the end block, and the other end of the second spring is fixedly connected to the guide rod. The guide rod can be connected and used by means of the second spring.
[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:
[0014] 1. This utility model pushes the installation cylinder into contact with the bottom of the inner side of the bottle. With the help of the clamping plate, the clamping groove, the spring and other structures, the installation cylinder is limited and stabilized. With the help of the partition, the internal growth space of the installation cylinder can be divided to improve the subsequent tissue culture diversity of plants.
[0015] 2. This utility model, through the setting of end balls, end rods and other structures, allows the sealing block to detach from the fixed plate, thereby allowing the fixed plate to be in a flow state. In conjunction with the setting of the ventilator, it can ensure normal air circulation and allow for timed ventilation and air exchange for tissue culture plants, avoiding the problem of dust and other particulate impurities easily generated by the open ventilator. Attached Figure Description
[0016] Figure 1 This is a perspective view of the overall structure of this utility model;
[0017] Figure 2 For the present utility model Figure 1 A schematic diagram of the internal structure of the bottle;
[0018] Figure 3 For the present utility model Figure 1 A front sectional view;
[0019] Figure 4 For the present utility model Figure 2 Enlarged view of point A;
[0020] Figure 5 For the present utility model Figure 3 Enlarged view of the ventilation mechanism.
[0021] In the diagram: 1. Bottle body; 2. Bottle cap; 3. Vent tube; 4. Vent mechanism; 5. Mounting cylinder; 6. Partition; 7. Slot; 8. Plate; 9. Protrusion; 10. Spring 1; 41. Fixing plate; 42. Sealing block; 43. End rod; 44. End ball; 45. End block; 46. Guide rod; 47. Spring 2. Detailed Implementation
[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0023] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 A tissue culture bottle for tissue culture seedlings includes a bottle body 1. A bottle cap 2 is threadedly connected to the inner wall of the bottle body 1. A vent tube 3 is fixedly connected to the inner wall of the bottle cap 2. The vertical part of the vent tube 3 is located inside the bottle body 1. An installation cylinder 5 is in contact with the bottom of the inner side of the bottle body 1. A partition 6 is fixedly connected to the inner wall of the installation cylinder 5. A slot 7 is provided on the side of the installation cylinder 5. A retaining plate 8 is slidably connected to the inner wall of the slot 7. A spring 10 is welded to the end face of the retaining plate 8 away from the partition 6. The other end of the spring 10 is fixedly connected to the bottle body 1.
[0024] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 There are two slots 7, which are symmetrically distributed on the mounting cylinder 5. The slots 7 facilitate insertion and use with the card plate 8. The surface of the card plate 8 is fixedly connected with protrusions 9, which are made of rubber. The protrusions 9 facilitate the movement of the card plate 8, and the rubber material has good durability.
[0025] Please see Figure 1 , Figure 2 , Figure 3 , Figure 5A ventilation mechanism 4 is provided on the horizontal part of the ventilation pipe 3. The ventilation mechanism 4 includes a fixed plate 41. The fixed plate 41 is fixedly connected to the horizontal part of the ventilation pipe 3. A sealing block 42 is slidably connected to the inner wall of the fixed plate 41. An end rod 43 is fixedly connected to the right end of the sealing block 42. An end block 45 is fixedly connected to the inner wall of the ventilation pipe 3. A guide rod 46 is fixedly connected to the side of the end rod 43. The guide rod 46 is slidably connected to the ventilation pipe 3. A second spring 47 is provided on the outer side of the end rod 43. Through the cooperation of the end rod 43, the second spring 47 and other structures, the sealing block 42 can be disengaged from the fixed plate 41, thereby ensuring the ventilation effect of the ventilation pipe 3.
[0026] Please see Figure 1 , Figure 2 , Figure 3 , Figure 5 The right end of the end rod 43 is fixedly connected to an end ball 44, which is made of silicone. The end ball 44 facilitates the movement of the end rod 43 by pulling it, and the silicone material has a good skin-friendly feel. There are two guide rods 46, which are symmetrically distributed on the end rod 43. The guide rods 46 guide the movement of the end rod 43. One end of the second spring 47 is fixedly connected to the end block 45, and the other end of the second spring 47 is fixedly connected to the guide rod 46. The second spring 47 allows the guide rod 46 to be connected and used.
[0027] The specific implementation process of this utility model is as follows: In use, by pushing the mounting cylinder 5 to contact the inner bottom of the bottle body 1, the clamping plate 8 moves under the pressure of the arc surface at the lower end of the mounting cylinder 5, so as to push the spring-10 to deform, so that the clamping plate 8 contacts the surface of the mounting cylinder 5. Under the action of force, the clamping plate 8 slides along the surface of the mounting cylinder 5. When the clamping plate 8 slides into the slot 7, the spring-10 returns to its deformation, so as to push the clamping plate 8 into the slot 7, thereby limiting the mounting cylinder 5 and avoiding the shaking problem of the mounting cylinder 5. Under the action of the partition 6 inside the mounting cylinder 5, the tissue culture space of the mounting cylinder 5 can be divided to improve the diversity of plant tissue culture.
[0028] By pulling the end ball 44 to the right, the end rod 43 is moved, causing the guide rod 46 to slide along the inside of the vent tube 3. Under the action of the end block 45, the spring 47 is deformed, and finally the end rod 43 drives the sealing block 42 to detach from the fixed plate 41, thus opening the vent tube 3 and ensuring the normal exchange of air inside the bottle 1.
[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A tissue culture bottle for tissue culture seedlings, comprising a bottle body (1), characterized in that: The inner wall of the bottle body (1) is connected to the bottle cap (2) by a thread. The inner wall of the bottle cap (2) is fixedly connected to the vent tube (3). The vertical part of the vent tube (3) is located inside the bottle body (1). The horizontal part of the vent tube (3) is provided with a venting mechanism (4). The bottom of the inner side of the bottle body (1) is in contact with the mounting cylinder (5). The inner wall of the mounting cylinder (5) is fixedly connected to the partition plate (6). The side of the mounting cylinder (5) is provided with a slot (7). The inner wall of the slot (7) is slidably connected to the card plate (8). The end face of the card plate (8) away from the partition plate (6) is welded with a spring (10). The other end of the spring (10) is fixedly connected to the bottle body (1).
2. The tissue culture bottle for tissue culture seedlings according to claim 1, characterized in that: There are two slots (7), which are symmetrically distributed on the mounting cylinder (5).
3. The tissue culture bottle for tissue culture seedlings according to claim 1, characterized in that: The surface of the card plate (8) is fixedly connected with protrusions (9), which are made of rubber.
4. The tissue culture bottle according to claim 1, wherein: The ventilation mechanism (4) includes a fixed plate (41), the horizontal part of the ventilation tube (3) is fixedly connected to the fixed plate (41), the inner wall of the fixed plate (41) is slidably connected to a sealing block (42), the right end of the sealing block (42) is fixedly connected to an end rod (43), the inner wall of the ventilation tube (3) is fixedly connected to an end block (45), the side of the end rod (43) is fixedly connected to a guide rod (46), the guide rod (46) is slidably connected to the ventilation tube (3), and a spring (47) is provided on the outer side of the end rod (43).
5. The tissue culture bottle according to claim 4, wherein: The right end of the end rod (43) is fixedly connected to an end ball (44), which is made of silicone.
6. A tissue culture bottle for tissue culture seedlings according to claim 4, characterized in that: There are two guide rods (46), which are symmetrically distributed on the end rod (43).
7. The tissue culture bottle according to claim 4, wherein: One end of the second spring (47) is fixedly connected to the end block (45), and the other end of the second spring (47) is fixedly connected to the guide rod (46).
Citation Information
Patent Citations
Tissue culture bottle for tissue culture seedlings
CN213153361U