Constant-temperature automatic stirring developing box for developing solution

Abstract

The utility model discloses a developing box capable of automatically stirring developing liquid at constant temperature, which comprises a box body, the box body is provided with a first chamber, a second chamber and a top cover arranged on the box body, a central cylinder is arranged in the second chamber, a bearing box is arranged on the central cylinder, and the top cover is arranged on the bearing box. A constant temperature mechanism for maintaining the constant temperature of the developing solution and a stirring mechanism for stirring the developing solution are arranged around the central cylinder; the developing box disclosed by the utility model is simple in structure and reasonable in design, can refrigerate and continuously stir a developing solution, does not need manual intervention, and is good in developing uniformity; the central cylinder is convenient for fixing a biological membrane and preventing displacement; the temperature guide pipe is high in refrigerating efficiency and stable in temperature control; the top cover structure can shield light and prevent leakage, supports operation in a luminous environment, and improves developing efficiency and convenience.

Description

Technical Field

[0001] This utility model relates to the field of biological membrane developing box technology, specifically a developing box with constant temperature and automatic stirring of developing solution. Background Technology

[0002] Western blotting is a routine experimental technique that uses specific antibodies to identify proteins and semi-quantitatively analyze their expression differences. Its "front-end" process—electrophoretic separation, membrane transfer, blocking, and incubation with primary / secondary antibodies—is almost identical across different laboratories, all aiming to obtain a biofilm containing the target protein as a detection carrier. The difference lies in the final signal reading stage: some laboratories use biofilm-chemiluminescence (ECL + X-ray film), while others employ CCD / CMOS darkrooms for digital chemiluminescence imaging, and alternative methods include fluorescence scanning or chromogenic precipitation. Different methods lead to different equipment and operating environments, but the goal remains the same: to stably and uniformly convert the chemical signal on the membrane surface into an archiveable band image.

[0003] Traditional biomembrane-chemiluminescence synthesis remains widely used due to its low cost and high sensitivity. However, this approach relies on darkroom conditions and involves a chain of discrete devices connected in series, including tray-shaking incubator, light-shielding exposure box transport, and film washing and developing. The substrate reaction temperature fluctuates with room temperature, and the uniformity of stirring depends on the mechanical characteristics of the shaking incubator or manual handling. Membranes and biomembranes are susceptible to interference from light or positional deviations during transport. Furthermore, the chemical state of the developing and fixing solutions further affects strip quality. The combination of dispersed equipment, frequent manual handling, and unstable temperature control results in inconsistent strip depth, high background noise, and poor repeatability—a persistent problem. Utility Model Content

[0004] The purpose of this invention is to provide a developer box with constant temperature automatic stirring to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a constant-temperature automatic stirring developing box for developing solution, comprising a box body, the box body having a first chamber and a second chamber, and a top cover disposed on the box body, a central column disposed in the second chamber, a support box disposed on the central column, a constant-temperature mechanism for maintaining the constant temperature of the developing solution and a stirring mechanism for stirring the developing solution disposed around the central column; the stirring mechanism comprising: a motor disposed in the first chamber, the motor output end having a main gear; a plurality of rotating shafts, the rotating shafts being rotatably disposed in the box body around their own axes, a portion of each rotating shaft being located in the second chamber, and a plurality of stirring plates for stirring the developing solution disposed on its sidewall; one end of each rotating shaft extending into the first chamber and having a secondary gear meshing with the main gear.

[0006] By setting up a constant temperature mechanism and an automatic stirring mechanism, the developer can maintain a constant temperature and be continuously stirred at a slow speed during the development process, resulting in a more stable development effect; the carrier box of the central column is used to fix and place the biofilm, preventing the biofilm from drifting or being damaged during the development process.

[0007] Preferably, the temperature control mechanism is a temperature-conducting tube, the end of which is connected to a cold source device, and the temperature-conducting part of the temperature-conducting tube is disposed in the second chamber.

[0008] Preferably, a heat insulation plate is provided between the heat-conducting pipe and the central column.

[0009] By using a temperature-conducting tube as a temperature-regulating mechanism and placing its temperature-conducting part inside the second chamber, the temperature of the developer can be controlled, thereby reducing the temperature of the developer.

[0010] Preferably, the central column has a threaded end facing the top cover. The top cover includes: a cover body, which is disposed on the box body and has a notch at its edge; a shaped plate, which is fixedly connected to the cover body, with its middle part protruding towards the second chamber, and the edge of the shaped plate extending along the protruding part, forming a pyramid-shaped structure. A through hole is provided at the center of the protruding part of the shaped plate, and multiple first flow ports are provided around the through hole. A guide sleeve is provided inside the through hole; and a screw, which passes through the guide sleeve, and the thread at the end of the screw is adapted to the threaded end.

[0011] The top cover structure, through the combination design of the cover body and the irregular plate, maintains the airtightness while setting through holes and guide sleeve structure. With the help of screw control for opening and closing, it not only facilitates the addition and discharge of developer, but also effectively blocks external light and prevents light from entering the developing chamber, thus ensuring that the developing process can be carried out in a light environment. It is easy to operate and has high developing efficiency.

[0012] Preferably, the edge portion of the irregularly shaped plate has multiple second flow openings, and in a top view, all the second flow openings are blocked by the cover and are not located within the visible range corresponding to the notch in the cover.

[0013] Preferably, the carrier box includes: a tray, which is fixedly disposed on the central column and has flow holes; and a lid, which is disposed on the tray. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the internal structure of the present invention from a downward viewing angle;

[0016] Figure 3 This is a top-view schematic diagram of the internal structure of this utility model;

[0017] Figure 4 This is a schematic diagram of the internal structure of the second chamber of this utility model;

[0018] Figure 5 This is the three-dimensional structure of the top cover edge after being cut apart according to this utility model;

[0019] Figure 6 This is a schematic diagram of the internal structure of the carrier box of this utility model.

[0020] In the diagram: 1. Box body; 2. First chamber; 3. Second chamber; 4. Top cover; 401. Cover body; 402. Notch; 403. Irregular plate; 404. Through hole; 405. Guide sleeve; 406. First flow port; 407. Screw; 5. Central column; 6. Thermostatic mechanism; 601. Temperature conducting pipe; 7. Stirring mechanism; 701. Motor; 702. Main gear; 703. Rotating shaft; 704. Stirring plate; 705. Secondary gear; 8. Insulation plate; 9. Thread; 10. Second flow port; 11. Carrier box; 1101. Support box; 1102. Flow hole; 1103. Box cover. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0023] In the description of this patent, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integrated connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this patent according to the specific circumstances.

[0024] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "several" means two or more, unless otherwise explicitly specified.

[0025] Example

[0026] Please see Figure 1-5 As shown, the present invention provides a developer solution constant temperature automatic stirring developing box technical solution: a developer solution constant temperature automatic stirring developing box includes a box body 1, the box body 1 is provided with a first chamber 2 and a second chamber 3, and a top cover 4 provided on the box body 1, a central column 5 is provided in the second chamber 3, a carrier box 1 is provided on the central column 5, and a constant temperature mechanism 6 for maintaining the constant temperature of the developer solution and a stirring mechanism 7 for stirring the developer solution are provided around the central column 5.

[0027] The stirring mechanism 7 includes components such as a motor 701 and several rotating shafts 703. The motor 701 is located in the first chamber 2, and the output end of the motor 701 is provided with a main gear 702. The rotating shafts 703 are rotatably arranged in the housing 1 around their own axes. A portion of each rotating shaft 703 is located in the second chamber 3, and its side wall is provided with multiple stirring plates 704 for stirring the developer. One end of the rotating shaft 703 extends into the first chamber 2 and is provided with a secondary gear 705 that meshes with the main gear 702.

[0028] In this embodiment, the motor 701 is a low-speed stable motor, such as a DC geared motor, to ensure smooth and reliable operation.

[0029] By setting up a constant temperature mechanism 6 and a stirring mechanism 7, the developer can maintain a constant temperature and be continuously stirred during the development process, eliminating the need for manual stirring or shaking, making the operation more convenient and the development effect more stable; the central column 5 is used to fix and place the biofilm, preventing the biofilm from drifting or being damaged during the development process.

[0030] The temperature control mechanism 6 is a temperature-conducting pipe 601. Part of the heat conduction of the temperature-conducting pipe 601 is located in the second chamber 3. The other end of the temperature-conducting pipe 601 is connected to a cold source device (not shown in the figure) to achieve the function of temperature control.

[0031] In this embodiment, the cold source device can be a small semiconductor refrigeration chip. The refrigeration section of the refrigeration chip is directly attached to / wrapped in the end of the temperature-conducting pipe 601 to achieve the refrigeration effect. The temperature-conducting pipe 601 can be a copper pipe in the prior art.

[0032] A heat insulation plate 8 is provided between the heat-conducting tube 601 and the central column 5. This design can effectively prevent the temperature generated by the heat-conducting tube 601 from being directly conducted to the biofilm, preventing the biofilm from becoming too cold and causing a decrease in reaction rate or failure.

[0033] By using a temperature-conducting tube 601 as a temperature-regulating mechanism 6 and placing its temperature-conducting part inside the second chamber 3, the temperature of the developer can be controlled and the temperature of the developer can be reduced.

[0034] like Figures 1-5 As shown, the central column 5 has a thread 9 at one end facing the top cover 4. The top cover 4 includes components such as a cover body 401, a shaped plate 403, and a screw 407. The cover body 401 is mounted on the box body 1 and has a notch 402 at its edge. The shaped plate 403 is fixedly connected to the cover body 401, with its middle part protruding towards the second chamber 3. The edge of the shaped plate 403 extends along the protruding part, forming a pyramidal structure. A through hole 404 is provided at the center of the protruding part of the shaped plate 403. Multiple first flow ports 406 are provided around the through hole 404. A guide sleeve 405 is provided inside the through hole 404. The screw 407 is rotatably inserted into the guide sleeve 405. The thread at the end of the screw 407 is adapted to the thread 9.

[0035] In the specific implementation process, a sealing ring can also be set at the edge of the cover 401 to prevent liquid from flowing out when it is poured.

[0036] The top cover 4 of this structure, through the combination design of the cover body 401 and the irregular plate 403, maintains the sealing while setting through holes 404 and guide sleeves 405. With the help of screws 407 to control opening and closing, it not only facilitates the addition and discharge of developer, but also effectively blocks external light and prevents light from entering the second chamber 3 for development, thereby ensuring that the process development can be carried out in the presence of light in the environment, making the operation convenient and the development efficiency high.

[0037] The edge of the irregular plate 403 has multiple second flow ports 10. This design ensures that when viewed from above, all second flow ports 10 are blocked by the cover 401 and are not located within the visible range corresponding to the notch 402 of the cover 401.

[0038] The carrier box 11 includes components such as a tray 1101 and a lid 1103. The tray 1101 is fixedly installed on the central column 5. A flow hole 1102 is opened on the tray 1101. The lid 1103 is detachably installed on the tray 1101.

[0039] In this embodiment, the bottom distance between the tray 1101 and the second chamber 3 can be adaptively reduced to facilitate development operations with a small amount of developer. The edge of the lid 1103 is provided with a positioning angle. In addition, magnetic strips are provided inside the tray 1101 and the lid 1103 for further fixation after installation.

[0040] The working principle of this utility model is as follows:

[0041] In this embodiment, the operator first places the biofilm in the tray 1101 and closes the lid 1103. Then, the screw 407 is threaded onto the central column 5 and tightened, so that the biofilm is in the closed space of the second chamber 3. Next, the operator pours the developing solution into the second chamber 3 through the notch 402 of the top cover 401. Under the guidance of the shaped plate 403, the developing solution flows into the second chamber 3 through the first flow port 406 and into the tray 1101 through the flow hole 1102, completing the solution injection process.

[0042] During the development process, the temperature-conducting tube 601 cools and controls the temperature of the second chamber 3 under the drive of an external cold source, ensuring that the temperature is around 4°C. At the same time, the motor 701 drives the main gear 702 and the auxiliary gear 705 to mesh, thereby driving the rotating shaft 703 to rotate slowly. The stirring plate 704 stirs the developer solution evenly, making the temperature distribution of the developer solution in the chamber more uniform, which is beneficial to improving the development quality.

[0043] After development is complete, the operator turns the notch 402 to the downward position, allowing the developer to drain smoothly through the second flow port 10. The fixing operation can then be performed following the same steps. Finally, the top cover 4 is removed, the cartridge cover 1103 is opened, and the developed biofilm is taken out.

[0044] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A developer box with constant temperature and automatic stirring, characterized in that: The device includes a housing with a first chamber and a second chamber, and a top cover. A central column is located in the second chamber, and a support box is mounted on the central column. A thermostatic mechanism for maintaining a constant temperature of the developer and a stirring mechanism for agitating the developer are located around the central column. The stirring mechanism includes: a motor located in the first chamber, with a main gear at its output end; several rotating shafts rotatably disposed within the housing, each shaft partially located in the second chamber, and having multiple agitator plates on its sidewalls for stirring the developer; one end of each rotating shaft extends into the first chamber and is connected to a secondary gear meshing with the main gear.

2. The constant temperature automatic developing box according to claim 1, wherein: The temperature control mechanism is a temperature-conducting tube, and a cold source device is connected to the end of the temperature-conducting tube. The temperature-conducting part of the temperature-conducting tube is located in the second chamber.

3. The constant temperature automatic developing tank according to claim 2, wherein: A heat insulation plate is provided between the heat-conducting pipe and the central column.

4. The constant temperature automatic developing box according to claim 1, characterized in that: The central column has a threaded end facing the top cover. The top cover includes: a cover body, which is disposed on the box body and has a notch at its edge; a shaped plate, which is fixedly connected to the cover body, with its middle part protruding towards the second chamber, and the edge of the shaped plate extending along the protruding part, forming a pyramid-shaped structure. A through hole is provided at the center of the protruding part of the shaped plate, and multiple first flow ports are provided around the through hole. A guide sleeve is provided inside the through hole; and a screw, which passes through the guide sleeve, and the end of the screw is adapted to the thread.

5. The constant temperature automatic developing tank according to claim 4, wherein: The irregularly shaped plate has multiple second flow openings on its edge, and these second flow openings are all blocked by the cover when viewed from above.

6. The constant temperature automatic developing box according to claim 1, wherein: The carrier box includes: a tray, which is fixedly mounted on the central column and has flow holes; and a lid, which is mounted on the tray.

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