An ex vivo leaf inoculation and moisturizing device
By designing sliders and pressure plates inside the box to hold the leaves and petioles, and combining transparent and opaque plates to control light, the problems of cumbersome leaf movement and water replenishment in traditional devices are solved, achieving stable moisture retention and efficient cultivation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHINA NAT TOBACCO CORP HENAN PROVINCIAL CO
- Filing Date
- 2025-05-21
- Publication Date
- 2026-06-12
AI Technical Summary
Traditional detached leaf humidification devices are prone to leaf movement during transportation and light exposure, resulting in poor humidification effects. Watering the petioles is cumbersome, and the alternation of light and dark cannot be effectively controlled, affecting the cultivation results.
A device comprising a box, a cover, a slider support, a slider, a pressure plate, and a light control plate was designed. The slider and pressure plate clamp the leaf and petiole to achieve stable fixation. The light is controlled by alternating transparent and opaque plates. The liquid addition slot allows for convenient water replenishment, ensuring full contact between the leaf and the culture paper.
This method achieves stable fixation of leaves during transportation, improves moisture retention, simplifies the petiole watering process, facilitates alternating light and dark cultivation, and enhances the cultivation efficiency of detached leaves.
Smart Images
Figure CN224343958U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of in vitro inoculation technology, specifically to an in vitro leaf inoculation and moisturizing device. Background Technology
[0002] In the process of studying the pathogenicity of pathogens, some plants require the removal of plant leaves for in vitro inoculation. This requires moisturizing the plant leaves for culture. The traditional method is to first collect the leaves, trim, disinfect, and clean their surfaces, then place the leaves in a box with sterile culture paper at the bottom. The leaves are placed with their upper surface facing down, and the petioles are wrapped with sterile absorbent cotton. The absorbent cotton is then moisturized with sterile water. The container is then sealed with plastic film or cling film. Subsequently, alternating light and dark conditions are implemented, and the temperature is controlled until the leaves meet the requirements for transplanting.
[0003] Traditional humidification equipment has several drawbacks: First, the leaves, lacking proper clamping and fixation, are prone to movement during transport and handling. Furthermore, the lack of contact between the leaflets facing down and the culture paper reduces humidification effectiveness. Second, the petioles, wrapped in absorbent cotton, require regular replenishment of sterile water. Due to light exposure and other cultivation steps, water evaporates and is lost, necessitating the removal of the plastic wrap for liquid replenishment. While the plastic wrap helps prevent evaporation, this replenishment step remains essential, making the traditional process cumbersome. The petiole is significantly thicker than the leaf, and without proper fixation and replenishment, the final cultivation outcome is negatively impacted. Third, traditional leaf containers often use fully transparent boxes, which accelerate water evaporation during light exposure. The cultivation process requires alternating light and dark periods, and traditional boxes fail to effectively block light and meet the requirements of light exposure, leading to cultivation failure.
[0004] Therefore, a simple structure, easy operation, obvious fixation effect, no leaf movement, obvious leaf surface contact effect, convenient and quick water replenishment at the petiole position, obvious petiole fixation and clamping effect, which is conducive to the scientific and reasonable distribution of leaves and facilitates alternating light and dark culture, is provided for the in vitro leaf inoculation and moisturizing device, which has broad market prospects. Utility Model Content
[0005] To address the shortcomings of existing technologies, this utility model provides an in vitro leaf inoculation and moisturizing device that is simple in structure, easy to operate, has a significant fixing effect, prevents leaf movement, provides a significant leaf surface contact effect, facilitates quick and easy water replenishment at the petiole position, has a significant petiole fixing and clamping effect, is conducive to the scientific and reasonable distribution of leaves, and is convenient for alternating light and dark culture, thus overcoming the deficiencies in existing technologies.
[0006] The technical solution of this utility model is implemented as follows: an in vitro leaf inoculation and moisturizing device includes a box body and a cover body installed on the top of the box body. Two symmetrical slider support seats are fixedly installed on two corresponding inner walls of the box body. Each slider support seat has a slider longitudinally engaged. The bottom of the slider is fixedly connected to a pressure plate. A liquid adding slot is opened on the cover body. An openable cover is installed on the liquid adding slot. A light control plate is provided on the cover body on one side of the liquid adding slot.
[0007] Furthermore, the box body has a square box structure, with an outer edge at the top of the box body, and a fastening piece that matches the outer edge is fixedly installed on the outer side of the cover.
[0008] Furthermore, one side of the slider support is fixedly connected to the inner wall of the box. A square through hole is provided in the longitudinal direction of the slider support. A sliding limit through hole is provided on the outer side of the square through hole. The slider is a square block structure. The slider is installed in the square through hole. A push rod fixedly connected to the outer wall of the slider is installed in the sliding limit through hole.
[0009] Furthermore, the length of the slider is matched with the length of the slider support, the installation direction of the slider is perpendicular to the bottom surface of the box, and the bottom surface of the slider support is located 3-5 cm above the bottom surface of the box.
[0010] Furthermore, the pressure plate is a square plate structure, the width of the pressure plate is equal to the width of the slider support, the width of the pressure plate is not less than the width of the slider, the bottom of the slider is vertically fixed to the top of one side of the pressure plate, and one side of the pressure plate is in contact with the inner wall of the box.
[0011] Furthermore, the pressure plate is parallel to the bottom surface of the box, and the length of the pressure plate is one-sixth of the length of the box.
[0012] Furthermore, the liquid adding slot is a directional through-hole structure, and the top surface of the liquid adding slot is provided with a raised edge. The cover is installed on the raised edge. The length of the liquid adding slot is not less than the distance between the two pressure plates. The opening position of the liquid adding slot corresponds perpendicularly to the setting position of the two pressure plates.
[0013] Furthermore, there are two light control boards, one of which is a transparent plastic board and the other is an opaque board. The light control board has a square plate structure, and a square mounting slot is provided on the box for installing the light control board. The two light control boards are alternately and movably engaged in the square mounting slot.
[0014] Furthermore, the box is 30 cm long, 24 cm wide, and 10 cm high.
[0015] This utility model has the following positive effects:
[0016] 1. This product uses a clamping plate to hold and fix some of the leaves and petioles. During the inoculation and moisturization process of detached leaves, it can ensure full contact between the front and bottom of the leaf and the culture paper, and ensure that the leaves will not shift during the movement of the box, thus enhancing the moisturization and culture effect.
[0017] 2. This product features a clamping and fixing mechanism at the petiole location. Unlike traditional methods where the petiole is completely wrapped in absorbent cotton, this product uses two layers of clamping cotton under the pressure plate during later use. The petiole is clamped between these two layers of sterile absorbent cotton, and the pressure plate presses down on the sides of the clamping cotton layers. This not only ensures that sterile water can reach the petiole after addition but also further secures the petiole. The entire box has a sealed structure, resulting in slow evaporation. Even if evaporation occurs, water can be precisely added to the clamping cotton layers through the liquid addition slot without opening the lid, thus improving work and operating efficiency.
[0018] 3. This product incorporates light control on the box body. A square mounting slot is provided on the top of the box body, and a light control board is installed accordingly. The light control board consists of two alternating plate-like structures, one for light transmission and the other for light blocking. It can provide light transmission during light transmission and block light when darkness is required. Unlike traditional transparent boxes, this product facilitates light control and improves inoculation and moisture retention efficiency. Attached Figure Description
[0019] Figure 1 This is a top view of the structure of this utility model.
[0020] Figure 2 This is a schematic diagram of the main structure of this utility model.
[0021] Figure 3 This is a schematic diagram of the internal structure of the present invention from the front view.
[0022] Figure 4 This is a top view of the internal structure of this utility model.
[0023] Figure 5 This is a top view of the structure of the present invention in use.
[0024] Figure 6 This is a front view structural diagram of the utility model in use. Detailed Implementation
[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0026] In the following description of the utility model, it should be noted that the terms "upper," "lower," "left," "right," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation. The term "connection" only indicates the connection between devices and has no special meaning.
[0027] Example 1: As Figure 1 , 2 As shown in Figures 3 and 4, an in vitro leaf inoculation and humidification device includes a box body 1 and a cover body 3 installed on the top of the box body 1. Two symmetrical slider support seats 8 are fixedly installed on two corresponding inner walls of the box body 1. Each slider support seat 8 has a slider 16 longitudinally engaged. The bottom of the slider 16 is fixedly connected to a pressure plate 10. A liquid adding slot hole 5 is opened on the cover body 3. An opening and closing cover 6 is installed on the liquid adding slot hole 5. A light control plate 7 is provided on the cover body 3 on one side of the liquid adding slot hole 5.
[0028] Specifically, the box body 1 is a container for detached leaves, and the cover body 3 is used in conjunction with the box body 1 to isolate and hold the leaves. The slider support 8 is a component of the slider 16, which can move vertically up and down within the slider support 8. When the slider 16 moves down, the pressure plate 10 moves down accordingly. The liquid addition slot 5 is designed to add liquid to the petiole area inside the box body without opening the cover body 3, maintaining leaf humidity and achieving moisturized cultivation of the leaves. The installation of the light control board 7 meets the requirements for both light and darkness, achieving efficient light control.
[0029] The box body 1 is a square box structure. An outer edge 2 is provided on the top of the box body 1. A fastening piece 4 that cooperates with the outer edge 2 is fixedly installed on the outer side of the cover 3. One side of the slider support 8 is fixedly connected to the inner wall of the box body 1. A square through hole is opened in the longitudinal direction of the slider support 8. A sliding limit through hole is provided on the outer side of the square through hole. The slider 16 is a square block structure. The slider 16 is installed in the square through hole. A push rod 9 that is fixedly connected to the outer wall of the slider 16 is installed in the sliding limit through hole.
[0030] Specifically, the slider 16 is installed in the square through hole, and the push rod 9 is used for hand operation to improve the stability of the slider 16 during operation, while ensuring that the slider 16 can drive the pressure plate to press down when it moves down. During use, the pressure plate 10 clamps the two layers of clamping cotton at both ends, fixing the two layers of clamping cotton, and then fixing the petiole. This clamping state only applies to the two ends of the clamping cotton layers and does not apply pressure to fix the petiole placed in the middle, thus fixing the petiole without damaging its structure.
[0031] The length of the slider 16 matches the length of the slider support 8. The installation direction of the slider 16 is perpendicular to the bottom surface of the box 1. The bottom surface of the slider support 8 is located 3-5 cm above the bottom surface of the box 1. The pressure plate 10 is a square plate structure. The width of the pressure plate 10 is equal to the width of the slider support 8, and the width of the pressure plate 10 is not less than the width of the slider 16. The bottom of the slider 16 is vertically fixed to the top of one side of the pressure plate 10, and one side of the pressure plate 10 is in contact with the inner wall of the box 1.
[0032] Specifically, the slider 16 moves along the vertical direction of the box, causing the pressure plate 10, which is parallel to the bottom of the box, to move. During the movement, the pressure plate 10 remains parallel to the bottom plane of the box. The pressure plate is limited to lift and lower, ensuring that the leaves placed along the width of the box will not move during subsequent operations, and ensuring that the front of the leaves adheres to the culture paper layer with culture medium adsorbed on the bottom.
[0033] The pressure plate 10 is parallel to the bottom surface of the box body 1, and the length of the pressure plate 10 is one-sixth of the length of the box body 1. The liquid adding slot 5 is a directional through-hole structure, and the top surface of the liquid adding slot 5 is provided with a raised edge. The cover 3 is installed on the raised edge. The length of the liquid adding slot 5 is not less than the distance between the two pressure plates 10, and the opening position of the liquid adding slot 5 corresponds perpendicularly to the setting position of the two pressure plates 10. There are two light control plates 7, one is a transparent plastic plate and the other is an opaque plate. The light control plate 7 has a square plate structure, and a square mounting slot is opened on the box body 1 for mounting the light control plate 7. The two light control plates 7 are alternately and movably engaged in the square mounting slot. The box body 1 is 30 cm long, 24 cm wide, and 10 cm high.
[0034] Specifically, the length of the pressure plate 10 should not be too long. If it is too long, it will affect the later use and cause damage to the leaf petiole structure held by the pressure plate. At the same time, it will reduce the space for placing the leaves. This patent uses a box of the above-mentioned size to store the leaves during the in vitro leaf inoculation and moisturization operation. It can effectively implement the spatial layout. When the leaves are installed, they are arranged in a fixed direction or at a fixed interval, which can not only improve the stability of the leaves, but also improve the inoculation and moisturization efficiency.
[0035] In actual operation, after opening the box lid, the harvested plant leaves 12 are trimmed, cleaned, and sterilized, and then placed at the bottom of the box 1 for in vitro inoculation and moisturization. A layer of culture paper 11 containing culture medium is first laid at the bottom of the box 1, and then the leaves are placed face down on the culture paper 11. A first layer of retaining cotton 14 is laid below the petiole 13, and the petiole 13 is placed on the first layer of retaining cotton 14. A second layer of retaining cotton 14 is then laid on top of the petiole 13. These two layers of retaining cotton 14 hold the petiole 13 and the portion of the leaf near the petiole 13 in place, ensuring the overall stability of the leaf 12.
[0036] After the leaf is placed, push the push rod downwards. The push rod 9 drives the slider 16 to move downwards along the square through hole longitudinally opened in the slider support seat. At the same time, the pressure plate 10 moves downwards, applying pressure to the two ends of the two layers of clamping cotton 14 to fix them in place, ensuring that the two layers of clamping cotton 14 will not move. Add sterile water to the culture paper layer 11 and the clamping cotton layer 14. Then put on the cover and perform moisturizing operations as needed. This product can maintain the condition until the work related to the pathogenicity of the detached leaf is completed.
[0037] When implementing alternating light and dark operation, if liquid evaporation necessitates water replenishment, simply open the cover 6 and add sterile liquid through the liquid addition slot 5; there is no need to open the cover 3. During light operation, a light-transmitting light control plate 7, made of transparent plastic, is installed within the square mounting slot. When light-shielding operation is required, an opaque light control plate 7, also made of opaque material, is installed within the square mounting slot.
[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A device for moisturizing detached leaf inoculation, comprising a box (1) and a cover (3) mounted on the top of the box (1), characterized in that: Two symmetrical slider support seats (8) are fixedly installed on the two corresponding inner walls of the box body (1). Each slider support seat (8) has a slider (16) longitudinally engaged. The bottom of the slider (16) is fixedly connected to the pressure plate (10). A liquid adding slot (5) is opened on the cover body (3). An opening and closing cover (6) is installed on the liquid adding slot (5). A light control plate (7) is provided on the cover body (3) on one side of the liquid adding slot (5).
2. The detached leaf inoculation and moisture retention device according to claim 1, characterized in that: The box body (1) is a square box structure. An outer edge (2) is provided on the top of the box body (1), and a fastening piece (4) that matches the outer edge (2) is fixedly installed on the outer side of the cover (3).
3. The detached leaf inoculation and moisture retention device according to claim 1, characterized in that: One side of the slider support (8) is fixedly connected to the inner wall of the box (1). A square through hole is provided in the longitudinal direction of the slider support (8). A sliding limit through hole is provided on the outside of the square through hole. The slider (16) is a square block structure. The slider (16) is installed in the square through hole. A push rod (9) fixedly connected to the outer wall of the slider (16) is installed in the sliding limit through hole.
4. The detached leaf inoculation and moisture retention device according to claim 1 or 3, characterized in that: The length of the slider (16) matches the length of the slider support (8). The installation direction of the slider (16) is perpendicular to the bottom surface of the box (1). The bottom surface of the slider support (8) is located 3-5 cm above the bottom surface of the box (1).
5. The detached leaf inoculation and moisture retention device according to claim 1, characterized in that: The pressure plate (10) is a square plate structure. The width of the pressure plate (10) is equal to the width of the slider support (8). The width of the pressure plate (10) is not less than the width of the slider (16). The bottom of the slider (16) is vertically fixed to the top of one side of the pressure plate (10). One side of the pressure plate (10) is in contact with the inner wall of the box (1).
6. The detached leaf inoculation and moisture retention device according to claim 1 or 5, characterized in that: The pressure plate (10) is parallel to the bottom surface of the box (1), and the length of the pressure plate (10) is one-sixth of the length of the box (1).
7. The detached leaf inoculation and moisture retention device according to claim 1, characterized in that: The liquid adding slot (5) is a directional through hole structure. The top surface of the liquid adding slot (5) is provided with a raised edge. The cover (3) is installed on the raised edge. The length of the liquid adding slot (5) is not less than the distance between the two pressure plates (10). The opening position of the liquid adding slot (5) is perpendicular to the setting position of the two pressure plates (10).
8. The detached leaf inoculation and moisture retention device according to claim 1, characterized in that: There are two light control boards (7), one is a transparent plastic board and the other is an opaque board. The light control board (7) has a square plate structure. A square mounting slot is provided on the box body (1) for installing the light control board (7). The two light control boards (7) are alternately and movablely engaged in the square mounting slot.
9. The detached leaf inoculation and moisture retention device according to claim 2, characterized in that: The box (1) is 30 cm long, 24 cm wide, and 10 cm high.