A lactobacillus plantarum strain propagation and cultivation device

Abstract

The application relates to the technical field of strain fermentation processes, and discloses a lactobacillus plantarum strain propagation and cultivation device, which comprises a base frame, a group of test tube placing assemblies arranged in linear equidistance on the upper surface of the base frame through a hinging device. The lactobacillus plantarum strain propagation and cultivation device adopts a 90-degree self-locking hinge as the hinging device, when the test tube is vertically cultured, the hinge is automatically locked to the limiting frame, accidental toppling caused by external force or vibration is effectively avoided, and the safety of the culture process is ensured; when the test tube is obliquely cultured, the clamping plate and the supporting plate are matched to support, and the stable support after the angle is fixed is realized by combining the adjustable clamping block positioning structure, test tube shaking or liquid leakage caused by accidental touch or gravity deviation is avoided.

Description

Technical Field

[0001] This application relates to the field of microbial fermentation technology, specifically to a device for propagating and cultivating Lactobacillus plantarum strains. Background Technology

[0002] In the food research and development process, it is necessary to conduct culture experiments on beneficial bacteria. Lactobacillus plantarum is a type of lactic acid bacteria that can produce lactic acid from sugars and utilize oxygen through respiration under aerobic conditions. This bacterium is widely distributed in nature and is often found on the surface of plants, in dairy products, meat products, fermented vegetables, and other environments. Lactobacillus plantarum has a variety of biological functions, such as regulating intestinal flora, enhancing immunity, and protecting the gastric mucosa.

[0003] An existing patent (publication number: CN217677508U) discloses a device for propagating and cultivating *Lactobacillus plantarum* strains, including a cultivation tube and a support base. The cultivation tube has a movable, breathable rubber plug at its opening. The support base includes a fixed base at the front and a movable base at the rear. The bottoms of the fixed and movable bases are rotatably connected by a damping hinge. The bottom edge of the movable base is arc-shaped, allowing it to rotate along a pivot and hover at any position between 0 and 90 degrees. Multiple first grooves are formed on the side adjacent to the fixed and movable bases, and multiple second grooves are formed on the side adjacent to the movable bases. The first and second grooves correspond one-to-one. When the fixed and movable bases are closed, the first and second grooves combine to form a space for the cultivation tube. This invention allows for free and arbitrary adjustment of the cultivation tube's tilt angle. When not in use, the fixed and movable bases can be closed. The device has a simple structure, is convenient and quick to use, and improves space utilization.

[0004] The aforementioned device connects the bottom of the fixed seat and the movable seat via a damping hinge, allowing for free and arbitrary adjustment of the culture tube's tilt angle. However, the stability after adjustment is low. If the test tube is accidentally touched by external force, the movable seat will continue to flip, causing the culture inside the test tube to drip or flow out. Furthermore, the device cannot adjust the angle of a single sample tube at will, making it unsuitable for different liquid levels inside the sample tube and lacking flexibility. Utility Model Content

[0005] To address the shortcomings of existing technologies, this application provides a device for propagating and cultivating Lactobacillus plantarum strains, which has advantages such as high stability and individual adjustment of sample tubes, thus solving the problems mentioned in the background technology.

[0006] To achieve the above objectives, this application provides the following technical solution: a device for propagating and cultivating Lactobacillus plantarum strains, including a base frame, wherein a set of test tube placement components arranged linearly at equal intervals are connected to the upper surface of the base frame via a hinge device;

[0007] The test tube placement assembly includes a limiting frame, an adaptive frame fixedly connected to the middle of the limiting frame, a limiting shaft rotatably connected between the left and right inner sidewalls of the adaptive frame, a limiting plate fixedly connected to the middle of the limiting shaft, the other end of the limiting plate located inside the limiting frame, two limiting wheels rotatably connected to the two sides of the bottom end of the limiting plate, and torsion springs installed between the two inner sidewalls of the adaptive frame and the limiting plate.

[0008] Furthermore, a bottom support plate is provided at the bottom of the limiting frame.

[0009] The above solution enables the bottom plate to limit the position of the sample tube.

[0010] Furthermore, the cross-section of the limiting frame is an isosceles trapezoid, and the limiting frame is perpendicular to the base frame.

[0011] The above scheme, by setting the cross-section of the limiting frame to an isosceles trapezoid, facilitates the placement of test tubes of different diameters.

[0012] Furthermore, a handle is fixedly connected to the front end of the base plate.

[0013] The above solution, with the addition of a handle, allows users to easily adjust the tilt angle of the limiting frame.

[0014] Furthermore, the hinge device is a 90-degree self-locking hinge.

[0015] With the above solution, when the limiting frame and the base frame are in a vertical state, the 90-degree self-locking hinge is in a self-locking state, preventing the limiting frame from accidentally tipping over due to external force or device vibration, and ensuring the stability of the test tube during vertical culture.

[0016] Furthermore, the inner rear wall of the limiting frame is provided with a scale groove.

[0017] With the above method, when the test tube is inserted, after the bottom of the test tube contacts the base plate, the user can quickly determine the inoculation amount or growth amount of Lactobacillus plantarum in the test tube by observing the relative position of the culture medium liquid level and the graduated groove in the test tube, so as to complete the volume estimation without pouring out the liquid.

[0018] Furthermore, a card plate is slidably inserted into the rear end of the base frame, and card blocks arranged linearly at equal intervals are provided on the upper surface of the card plate. A support plate is hinged to the back of the limiting frame.

[0019] With the above scheme, when the sample tube needs to be tilted, the limiting frame is flipped backward by unlocking the hinge device. After determining the flipping range, the bottom end of the hinged plate is placed between two adjacent plates to support the plate. When the flipping angle is large, the center of gravity of the device may shift backward. By pulling the plate backward, the bottom contact area of ​​the base frame can be increased, thereby improving stability.

[0020] Furthermore, both sides of the base frame are threaded with threaded posts for fixing the card plate.

[0021] The above method uses threaded posts to press against both sides of the clamping plate, thus fixing the clamping plate in place and preventing it from shifting on its own.

[0022] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0023] This Lactobacillus plantarum strain propagation and cultivation device uses a 90-degree self-locking hinge as the hinge device. When the test tube is cultured vertically, the hinge automatically locks the limit frame, effectively preventing accidental tipping caused by external force or vibration, and ensuring the safety of the culture process. When cultured at an angle, the device is supported by a clamping plate and a support plate, combined with an adjustable clamping block positioning structure, to achieve stable support after the angle is fixed, avoiding test tube shaking or liquid leakage caused by accidental touch or shift of the center of gravity.

[0024] Each test tube placement component can be operated independently, and the tilt angle can be adjusted individually according to the actual liquid level or culture needs of a single test tube, adapting to the personalized operation requirements of different experimental stages and solving the problem of insufficient flexibility caused by overall adjustment in the existing technology.

[0025] The limiting frame adopts an isosceles trapezoidal cross-section design. Combined with the torsion spring and limiting wheel, it can accommodate the fixing needs of test tubes of different diameters, improving the overall applicability.

[0026] The built-in graduated groove design allows for quick estimation of inoculum quantity or growth rate by measuring the relative position of the liquid surface and the graduations without tilting the test tube, improving user convenience. Attached Figure Description

[0027] Figure 1 This is a three-dimensional schematic diagram of the overall structure of this application. Figure 1 ;

[0028] Figure 2 This is a three-dimensional schematic diagram of the overall structure of this application. Figure 2 ;

[0029] Figure 3 This is a cross-sectional view of the test tube placement component structure in this application;

[0030] Figure 4 This is a top view of the test tube placement component structure of this application.

[0031] In the picture:

[0032] 1. Base frame; 2. Hinge assembly;

[0033] 3. Test tube placement assembly; 301. Limiting frame; 302. Adaptive frame; 303. Limiting shaft; 304. Limiting plate; 305. Limiting wheel; 306. Torsion spring;

[0034] 4. Base plate; 5. Handle; 6. Scale groove; 7. Clamping plate; 8. Clamping block; 9. Support plate; 10. Threaded column. Detailed Implementation

[0035] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0036] Please see Figure 1 , Figure 2 and Figure 4 The Lactobacillus plantarum strain propagation and cultivation device in this embodiment includes a base frame 1. The upper surface of the base frame 1 is connected by a set of test tube placement components 3 arranged linearly at equal intervals through a hinge device 2. The test tube placement components 3 include a limiting frame 301. A bottom plate 4 is provided at the bottom of the limiting frame 301. The cross-section of the limiting frame 301 is an isosceles trapezoid. The limiting frame 301 is perpendicular to the base frame 1. A handle 5 is fixedly connected to the front end of the bottom plate 4. The handle 5 allows the user to adjust the tilt angle of the limiting frame 301.

[0037] Please see Figure 1 , Figure 2 and Figure 3 The limiting frame 301 has an isosceles trapezoidal cross-section, which facilitates the placement of test tubes of different diameters. The base plate 4 can limit the bottom of the sample tube. An adaptive frame 302 is fixedly connected to the middle of the limiting frame 301. A limiting shaft 303 is rotatably connected between the left and right inner walls of the adaptive frame 302. A limiting plate 304 is fixedly connected to the middle of the limiting shaft 303. The other end of the limiting plate 304 is located inside the limiting frame 301. The bottom two sides of the limiting plate 304 are rotatably connected to... Two limiting wheels 305 are installed between the two inner sidewalls of the self-adaptive frame 302 and the limiting plate 304. Since the bottom of the test tube is arc-shaped, when the test tube is inserted from the top of the limiting frame 301, the bottom of the test tube presses against the two limiting wheels 305, which can cause the limiting plate 304 to flip outward. The limiting wheels 305 can abut against the surface of the test tube through the restoring force of the torsion spring 306, so that the surface of the test tube is in close contact with the inner wall of the limiting frame 301, thereby achieving the purpose of fixing the test tube.

[0038] Please see Figure 1 , Figure 2 and Figure 3 The hinge device 2 is a 90-degree self-locking hinge. The 90-degree self-locking hinge is a special hinge device that can automatically lock when opened to 90 degrees to prevent parts or devices from accidentally moving or closing due to external force or vibration. When the limiting frame 301 is in a vertical state with the base frame 1, the 90-degree self-locking hinge is in a self-locking state to prevent the limiting frame 301 from accidentally tipping over due to external force or device vibration, ensuring the stability of the test tube during vertical culture. The inner rear wall of the limiting frame 301 is provided with a graduated groove 6. When the test tube is inserted, after the bottom of the test tube contacts the base plate 4, the user can quickly determine the inoculation amount or growth amount of Lactobacillus plantarum in the test tube by observing the relative position of the culture medium liquid level in the test tube and the graduated groove 6, so as to complete the volume estimation without pouring out the liquid.

[0039] Please see Figure 1 , Figure 2 and Figure 3 The base frame 1 has a sliding insert plate 7 at its rear end. The upper surface of the plate 7 is provided with locking blocks 8 arranged linearly at equal intervals. The back of the limiting frame 301 is hinged with a support plate 9. When the sample tube needs to be tilted, the limiting frame 301 is flipped backward by unlocking the hinge device 2. After determining the flipping range, the bottom end of the hinged plate 7 is placed between two adjacent locking blocks 8 to support the plate 7. When the flipping angle is large, the center of gravity of the device may shift backward. By pulling the plate 7 backward, the bottom contact area of ​​the base frame 1 can be increased, thereby improving stability. Both sides of the base frame 1 are threaded with threaded posts 10. The pressure of the threaded posts 10 on both sides of the plate 7 can achieve the purpose of fixing the plate 7 and prevent the plate 7 from shifting on its own.

[0040] The working principle of the above embodiment is as follows: A set of equally spaced test tube placement components 3 are hinged to the upper surface of the base frame 1 by a 90-degree self-locking hinge. In the initial state, the limiting frame 301 is perpendicular to the base frame 1. At this time, the self-locking hinge is in an automatic locking state to prevent the limiting frame 301 from accidentally tipping over due to external force or vibration, ensuring the stability of the test tubes during vertical culture. When the test tube is inserted from the top of the limiting frame 301, the arc-shaped structure at the bottom of the test tube presses down on the limiting wheel 305. After being compressed, the limiting wheel 305 drives the limiting plate 304 to rotate outward around the limiting axis 303. At this time, the torsion spring 306 is compressed and stores elastic potential energy. When the test tube continues to move down to the bottom end and contacts the base plate 4, the restoring force of the torsion spring 306 drives the limiting wheel 305 to press tightly against the surface of the test tube, so that the outer wall of the test tube is in close contact with the inner wall of the limiting frame 301, realizing the self-adaptive fixation of the test tube. The isosceles trapezoidal design of the limiting frame 301 can accommodate different types of test tubes. Using test tubes of the same diameter enhances the versatility of the device. After the test tube is fixed, its bottom end is limited by the base plate 4. The relative position of the scale groove 6 on the inner rear wall of the limiting frame 301 and the liquid surface of the culture medium in the test tube can intuitively reflect the inoculation amount or growth amount of the bacteria. The volume can be estimated without tilting the test tube, reducing the risk of contamination. When it is necessary to adjust the tilt angle of the test tube, the 90-degree self-locking hinge is released, and the limiting frame 301 can be flipped backward. According to the experimental requirements, the limiting frame 301 is flipped to the target angle, and the bottom end of the support plate 9 hinged to the back of the limiting frame 301 is inserted into the gap of the upper surface of the card plate 7 slidingly inserted at the rear end of the base frame 1 to fix the angle. If the tilt angle is large and causes the center of gravity of the device to shift backward, the card plate 7 can be pulled backward to increase the contact area at the bottom of the base frame 1 and balance the center of gravity. Then, the threaded columns 10 on both sides of the base frame 1 are rotated to press the sides of the card plate 7 to prevent the card plate 7 from sliding and further improve stability.

[0041] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0042] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for propagating and cultivating Lactobacillus plantarum strains, comprising a base frame (1), characterized in that: The upper surface of the base frame (1) is connected by a set of test tube placement components (3) arranged linearly at equal distances through a hinge device (2); The test tube placement assembly (3) includes a limiting frame (301), an adaptive frame (302) is fixedly connected to the middle of the limiting frame (301), a limiting shaft (303) is rotatably connected between the left and right inner sidewalls of the adaptive frame (302), a limiting plate (304) is fixedly connected to the middle of the limiting shaft (303), the other end of the limiting plate (304) is located inside the limiting frame (301), two limiting wheels (305) are rotatably connected to the two sides of the bottom end of the limiting plate (304), and torsion springs (306) are installed between the two inner sidewalls of the adaptive frame (302) and the limiting plate (304).

2. The Lactobacillus plantarum strain propagation and cultivation device according to claim 1, characterized in that: The bottom of the limiting frame (301) is provided with a bottom plate (4).

3. The Lactobacillus plantarum strain propagation and cultivation device according to claim 2, characterized in that: The cross-section of the limiting frame (301) is an isosceles trapezoid, and the limiting frame (301) is perpendicular to the base frame (1).

4. The Lactobacillus plantarum strain propagation and cultivation device according to claim 2, characterized in that: The front end of the base plate (4) is fixedly connected to a handle (5). The handle (5) allows the user to adjust the tilt angle of the limiting frame (301).

5. The Lactobacillus plantarum strain propagation and cultivation device according to claim 1, characterized in that: The hinge device (2) is a 90-degree self-locking hinge.

6. The Lactobacillus plantarum strain propagation and cultivation device according to claim 2, characterized in that: The inner rear wall of the limiting frame (301) is provided with a scale groove (6).

7. The Lactobacillus plantarum strain propagation and cultivation device according to claim 1, characterized in that: The rear end of the base frame (1) is slidably connected to a card plate (7), and the upper surface of the card plate (7) is provided with card blocks (8) arranged linearly at equal distances. The back of the limiting frame (301) is hinged to a support plate (9).

8. The Lactobacillus plantarum strain propagation and cultivation device according to claim 7, characterized in that: Both sides of the base frame (1) are threaded with threaded posts (10) for fixing the card plate (7).

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