A high-temperature-resistant bacillus directional separation device in a Maotai-flavor liquor pit mud
By designing a separation device that includes a stirring rod, a temperature control component, and a robotic arm, the problem of mixed bacteria during the separation of thermostable Bacillus from baijiu cellar mud was solved, achieving efficient separation and purification of thermostable Bacillus from cellar mud.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHENGDU ALCOHOL IN ALCOHOL DISTILLERS YEAST CO LTD
- Filing Date
- 2025-07-07
- Publication Date
- 2026-06-12
AI Technical Summary
Existing equipment often mixes in mesophilic bacteria and molds when separating thermostable Bacillus from baijiu cellar mud, and the lack of temperature treatment makes it difficult to distinguish them during separation.
A separation device comprising a shell, a pretreatment chamber, an incubator, and an ultra-clean working chamber was designed. Through components such as a stirring rod, a temperature control component, a UV lamp, and a robotic arm, the device enables the removal of impurities, cooling, cultivation, and precise picking of Bacillus spores from the pit mud.
It enables convenient separation and precise extraction of thermoresistant Bacillus from pit mud, improving separation efficiency and purity.
Smart Images
Figure CN224350650U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of Bacillus processing technology, specifically a device for the directional separation of heat-resistant Bacillus from the cellar mud of soy sauce-flavored liquor. Background Technology
[0002] Bacillus is named for its rod-shaped and elliptical cells. Different species of Bacillus have different external shapes, with differences ranging from micrometers. They are widely found in plants, soil, and the intestines, and they divide relatively quickly. They are easily affected by the surrounding environment and have characteristics such as heat resistance and the ability to secrete enzymes.
[0003] In order to quickly isolate high-spore-resistant Bacillus from the mud of baijiu cellars, most devices directly inject the treated mud liquid onto a culture medium for cultivation, and then select it in a targeted manner. However, such separation devices often lack temperature treatment, and the bacteria are easily mixed with mesophilic bacteria and molds, which are not easy to distinguish during separation.
[0004] Now, a novel directional separation device for thermoresistant Bacillus spores in the cellar mud of Maotai-flavor liquor is proposed to solve the above problems. Utility Model Content
[0005] The purpose of this invention is to provide a directional separation device for thermoresistant Bacillus spores in the cellar mud of Maotai-flavor liquor, so as to solve the problem of bacterial contamination mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution: a directional separation device for thermoresistant Bacillus subtilis in the cellar mud of Maotai-flavor liquor, comprising a shell and a pretreatment chamber. The pretreatment chamber is fixed to the upper left corner of the outer shell, and a second material pump is mounted at the center of the top of the outer shell of the pretreatment chamber. A large geared disc is movably connected between the bottom of the second material pump and the pretreatment chamber. A small geared disc is movably connected to the left side of the center of the top of the outer shell of the pretreatment chamber. A motor is fixed to the left side of the center of the top of the top of the pretreatment chamber. A vortex tube is movably connected to the center of the top of the inner shell of the pretreatment chamber, and stirring rods are fixed to the lower sides of the outer sides of the vortex tube. A temperature control component is installed on the inner wall of the pretreatment chamber. A valve is fixed to the center of the bottom of the pretreatment chamber. An inclined platform is horizontally fixed at the upper part of the inner shell, and a cooling coil is laid obliquely inside the inclined platform. A cooling chamber is provided between the top of the inclined platform and the shell. A liquid pump is fixed between the left side of the cooling coil and the shell.
[0007] As a further technical solution of this utility model, the top of the output end of the motor is fixedly connected to the small gear disk, and the top of the rotary tube is fixedly connected to the large gear disk.
[0008] As a further technical solution of this utility model, an incubator is fixed on the left side inside the shell, and a cavity is provided between the right side of the incubator and the shell. An ultraviolet lamp is installed at the rear inside the cavity. A first material pump is installed between the right side of the top of the cavity and the inclined platform. A hose is fixed to the bottom of the first material pump, and a nozzle is fixed to the end of the hose. A retainer is fixed to the right side outside the incubator. A partition is installed between the two sides inside the incubator, and a petri dish is placed on the partition.
[0009] As a further technical solution of this utility model, the nozzle is embedded in the sleeve, and the partition is on the same vertical plane as the incubator.
[0010] As a further technical solution of this utility model, a cleanroom is fixed to the left side of the outer shell, and a robotic arm is installed at the top inside the cleanroom. A fixing block is movably installed at the bottom of the robotic arm, and a camera is fixed to the right side of the bottom of the fixing block, and an inoculation rod is fixed to the left side of the bottom of the fixing block.
[0011] As a further technical solution of this utility model, a placement platform is fixed at the bottom inside the ultra-clean work box, and sliders are movably connected to both sides inside the placement platform. A limit block is fixed at the top of the slider, and a bolt is threaded to the front side of the limit block. A screw hole is provided between the two sides at the front of the top of the placement platform.
[0012] Compared with the prior art, the beneficial effects of this utility model are: the directional separation device for heat-resistant Bacillus in the cellar mud of soy sauce-flavored liquor not only realizes the easy removal of miscellaneous bacteria and the integration of processing and cultivation, but also realizes the precise picking of Bacillus.
[0013] (1) Stirring rods are fixed on the lower sides of the outer side of the spiral tube. The second material pump injects the pit mud material into the pretreatment chamber through the large toothed disc and the spiral tube. The motor rotates the small toothed disc and pushes the large toothed disc and the spiral tube to rotate together. The temperature control component is used to remove impurities from the pit mud in the pretreatment chamber. After the process is completed, the valve is opened and the mud enters the cooling chamber. It flows along the inclined platform with the cooling coil to the first material pump to complete the cooling process of the pit mud material so that it can be coated with inoculum.
[0014] (2) An incubator is fixed on the left side inside the shell. After the first material pump is turned on, it is injected into the nozzle through a hose to open the incubator. The mixture of pit mud and bacterial solution is injected into the culture dish through the nozzle. A certain temperature is maintained in the incubator to achieve rapid growth of Bacillus spores so that bacteria can be selected for inoculation later. After the incubator is finished, the injection mechanism is disinfected with a UV lamp.
[0015] (3) By fixing an ultra-clean working box on the left side of the outer shell, the culture dish with Bacillus spores is placed on the placement platform. The slider is moved left and right along the cavity inside the placement platform. After aligning with the screw hole, the bolt is screwed in to reinforce it, which can restrict the culture dish in the center and prevent it from shaking. The inoculation rod can also be observed and selected by a camera through a flexible and extendable robotic arm. The bacterial culture is extracted in the ultra-clean working box and injected into a test tube for preservation. Attached Figure Description
[0016] Figure 1 This is a frontal cross-sectional view of the present invention.
[0017] Figure 2 This is a front view cross-sectional structural diagram of the pretreatment chamber of this utility model;
[0018] Figure 3 This is a front view cross-sectional structural diagram of the shell of this utility model;
[0019] Figure 4 This is a front view cross-sectional structural diagram of the ultra-clean work box of this utility model.
[0020] In the diagram: 1. Shell; 2. Hose; 3. UV lamp; 4. Cavity; 5. Sleeve; 6. Nozzle; 7. First material pump; 8. Cooling chamber; 9. Inclined platform; 10. Cooling coil; 11. Pretreatment chamber; 12. Second material pump; 13. Large gear disc; 14. Small gear disc; 15. Motor; 16. Rotary tube; 17. Temperature control component; 18. Stirring rod; 19. Valve; 20. Liquid pump; 21. Incubator; 22. Cleanroom; 23. Petri dish; 24. Partition; 25. Placement platform; 26. Robotic arm; 27. Fixing block; 28. Camera; 29. Inoculation rod; 30. Limiting block; 31. Bolt; 32. Slider; 33. Screw hole. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0022] Please see Figures 1 to 4This utility model provides an embodiment of a directional separation device for thermoresistant Bacillus spp. in the cellar mud of a soy sauce-flavored liquor, comprising a shell 1 and a pretreatment chamber 11. The pretreatment chamber 11 is fixed to the upper left corner of the shell 1, and a second material pump 12 is mounted at the center of the top of the pretreatment chamber 11. A large geared disc 13 is movably connected between the bottom of the second material pump 12 and the pretreatment chamber 11. A small geared disc 14 is movably connected to the left side of the center of the top of the pretreatment chamber 11. The left side of the center of the top of the pretreatment chamber 11 is fixed. A motor 15 is fixed. A vortex tube 16 is movably connected to the center of the top of the pretreatment chamber 11. Stirring rods 18 are fixed to the lower sides of the outer sides of the vortex tube 16. A temperature control component 17 is installed on the inner wall of the pretreatment chamber 11. A valve 19 is fixed to the center of the bottom of the pretreatment chamber 11. An inclined platform 9 is horizontally fixed to the upper part of the shell 1. A cooling coil 10 is laid obliquely inside the inclined platform 9. A cooling chamber 8 is set between the top of the inclined platform 9 and the shell 1. A liquid pump 20 is fixed between the left side of the cooling coil 10 and the shell 1.
[0023] The top of the output end of the motor 15 is fixedly connected to the small gear 14, and the top of the rotary tube 16 is fixedly connected to the large gear 13.
[0024] Specifically, such as Figure 1 and Figure 2 As shown, the second material pump 12 injects the pit mud material into the pretreatment chamber 11 through the large gear plate 13 and the spiral tube 16. The motor 15 rotates the small gear plate 14 and pushes the large gear plate 13 and the spiral tube 16 to rotate together. With the help of the temperature control component 17, the pit mud in the pretreatment chamber 11 is treated to remove impurities and bacteria. After the treatment is completed, the valve 19 is opened and the mud enters the cooling chamber 8. It flows along the inclined platform 9 with the cooling coil 10 to the first material pump 7 for inoculation.
[0025] An incubator 21 is fixed to the left side inside the shell 1, and a cavity 4 is provided between the right side of the incubator 21 and the shell 1. An ultraviolet lamp 3 is installed at the rear inside the cavity 4. A first material pump 7 is installed between the right side of the top of the cavity 4 and the inclined platform 9. A hose 2 is fixed to the bottom of the first material pump 7, and a nozzle 6 is fixed to the end of the hose 2. A retaining sleeve 5 is fixed to the right side outside the incubator 21. A partition 24 is installed between the two sides inside the incubator 21, and a petri dish 23 is placed on the partition 24. The nozzle 6 is embedded in the retaining sleeve 5. The partition 24 and the incubator 21 are on the same vertical plane.
[0026] Specifically, such as Figure 1 and Figure 3 As shown, after the first material pump 7 is turned on, it is injected into the nozzle 6 through the hose 2 in preparation for opening the incubator 21. Then, the mixture of pit mud and bacterial solution is injected into the culture dish 23 through the nozzle 6. A certain temperature is maintained in the incubator 21 to achieve rapid development and growth of Bacillus, so that bacteria can be selected for inoculation later, and then the bacteria are disinfected again.
[0027] A cleanroom 22 is fixed to the left side of the outer shell 1, and a robotic arm 26 is installed at the top inside the cleanroom 22. A fixing block 27 is movably installed at the bottom of the robotic arm 26, and a camera 28 is fixed to the right side of the bottom of the fixing block 27. An inoculation rod 29 is fixed to the left side of the bottom of the fixing block 27. A placement platform 25 is fixed to the lower part inside the cleanroom 22, and sliders 32 are movably connected to both sides inside the placement platform 25. A limit block 30 is fixed to the top of the slider 32, and a bolt 31 is threaded to the front side of the limit block 30. A screw hole 33 is provided between the two sides at the front of the top of the placement platform 25.
[0028] Specifically, such as Figure 1 and Figure 4 As shown, the culture dish 23 containing Bacillus spores is placed on the placement platform 25. The slider 32 is moved left and right along the cavity inside the placement platform 25. After aligning with the screw hole 33, the bolt 31 is screwed in to secure it. This can restrain the culture dish 23 in the center and prevent it from shaking. The inoculation rod 29 can be observed by the flexible and extendable robotic arm 26 and the camera 28 to select Bacillus spore colonies and inoculate them to other places.
[0029] Working Principle: In use, the second material pump 12 first injects the pit mud material into the pretreatment chamber 11 through the large gear disc 13 and the spiral pipe 16. The motor 15 rotates the small gear disc 14, pushing the large gear disc 13 and the spiral pipe 16 to rotate together. The temperature control component 17 then removes bacteria from the pit mud in the pretreatment chamber 11. After this process, the valve 19 is opened, and the mud enters the cooling chamber 8. It flows along the inclined platform 9 with the cooling coil 10 to the first material pump 7, completing the cooling process. After the first material pump 7 is turned on, the mud is injected into the nozzle 6 through the hose 2, ready to be opened in the incubator 21, and then further injected through the nozzle. The head 6 injects a mixture of pit mud and bacterial solution into the culture dish 23, and maintains a certain temperature in the incubator 21 to achieve rapid development and growth of Bacillus. Finally, the culture dish 23 with Bacillus is placed on the placement platform 25. The slider 32 is moved left and right along the cavity inside the placement platform 25. After aligning with the screw hole 33, the bolt 31 is screwed in to reinforce it, which can restrain the culture dish 23 in the center and prevent it from shaking. The inoculation rod 29 can be observed and selected by the flexible and extendable robotic arm 26 and the camera 28. The bacterial colonies are extracted in the ultra-clean work box 22 and injected into the test tube for preservation.
[0030] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
Claims
1. A device for the directional separation of thermoresistant Bacillus spp. from the cellar mud of a soy sauce-flavored liquor, comprising a shell (1) and a pretreatment chamber (11), characterized in that: A pretreatment chamber (11) is fixed to the upper left corner of the outer shell (1), and a second material pump (12) is mounted at the center of the top of the pretreatment chamber (11). A large gear plate (13) is movably connected between the bottom of the second material pump (12) and the pretreatment chamber (11). A small gear plate (14) is movably connected to the left side of the center of the top of the pretreatment chamber (11). A motor (15) is fixed to the left side of the center of the top of the pretreatment chamber (11). A rotary tube (16) is movably connected to the center of the top of the pretreatment chamber (11). Furthermore, stirring rods (18) are fixed below the outer sides of the spiral tube (16), a temperature control component (17) is installed on the inner wall of the pretreatment chamber (11), a valve (19) is fixed at the center of the bottom of the pretreatment chamber (11), a ramp (9) is fixed horizontally above the inside of the shell (1), and a cooling coil (10) is laid obliquely inside the ramp (9). A cooling chamber (8) is provided between the top of the ramp (9) and the shell (1), and a liquid pump (20) is fixed between the left side of the cooling coil (10) and the shell (1).
2. The device for the directional separation of thermoresistant Bacillus spores in the cellar mud of a sauce-flavored liquor as described in claim 1, characterized in that: The top of the output end of the motor (15) is fixedly connected to the small gear plate (14), and the top of the rotary tube (16) is fixedly connected to the large gear plate (13).
3. The device for the directional separation of thermoresistant Bacillus spores in the cellar mud of a sauce-flavored liquor as described in claim 1, characterized in that: An incubator (21) is fixed on the left side inside the shell (1), and a cavity (4) is provided between the right side of the incubator (21) and the shell (1). A UV lamp (3) is installed at the rear inside the cavity (4). A first material pump (7) is installed between the right side of the top of the cavity (4) and the inclined platform (9). A hose (2) is fixed at the bottom of the first material pump (7). A nozzle (6) is fixed at the end of the hose (2). A ferrule (5) is fixed on the right side outside the incubator (21). A partition (24) is installed between the two sides inside the incubator (21), and a petri dish (23) is placed on the partition (24).
4. The directional separation device for thermoresistant Bacillus spp. in the cellar mud of a sauce-flavored liquor as described in claim 3, characterized in that: The nozzle (6) is embedded in the sleeve (5), and the partition (24) and the incubator (21) are on the same vertical plane.
5. The directional separation device for thermoresistant Bacillus spores in the cellar mud of a sauce-flavored liquor as described in claim 1, characterized in that: A cleanroom chamber (22) is fixed to the left side of the outer shell (1), and a robotic arm (26) is installed at the top inside the cleanroom chamber (22). A fixing block (27) is movably installed at the bottom of the robotic arm (26), and a camera (28) is fixed to the right side of the bottom of the fixing block (27). An inoculation rod (29) is fixed to the left side of the bottom of the fixing block (27).
6. The device for the directional separation of thermoresistant Bacillus spores in the cellar mud of a sauce-flavored liquor as described in claim 5, characterized in that: A placement platform (25) is fixed at the bottom inside the ultra-clean work box (22), and sliders (32) are movably connected to both sides inside the placement platform (25). A limit block (30) is fixed at the top of the slider (32), and a bolt (31) is threadedly connected to the front side of the limit block (30). A screw hole (33) is provided between the two sides at the front of the top of the placement platform (25).