A kit for detecting a stem rust resistance gene

Abstract

This utility model relates to the technical field of rust detection kits, specifically a stripe rust resistance gene detection kit. The kit includes a reagent kit with a crushing tank, a storage tank, and a protective tank arranged sequentially from left to right at its upper end. A crushing barrel is installed on the inner wall of the crushing tank, and a cutting head is installed on the inner bottom wall of the crushing barrel. A micro-motor is detachably connected to the lower end of the crushing barrel, and the output end of the micro-motor passes through the bottom end of the crushing barrel and is engaged with the inner wall of the cutting head. This stripe rust resistance gene detection kit, through the coordinated arrangement of the crushing barrel, cutting head, and micro-motor, allows the operator to place the leaf sample into the crushing barrel when needed. The micro-motor then drives the cutting head to rotate, rapidly crushing the leaf sample. This allows for thorough fragmentation of the leaf tissue in a short time, enabling the rapid release of target substances such as nucleic acids from the cells.

Description

Technical Field

[0001] This utility model relates to the technical field of stripe rust detection kits, specifically a stripe rust resistance gene detection kit. Background Technology

[0002] Wheat stripe rust is a disease caused by the fungus *Strombus styracifolius*, which can occur from wheat emergence to maturity. It primarily affects the leaves, followed by the leaf sheaths and stems, and also infects the ears, glumes, and awns. In seedlings, bright yellow spots appear on the leaves. In mature plants, when the disease first appears, numerous bright yellow uredinia form on the upper surface of the leaves. Later in the disease, the leaf epidermis ruptures, releasing a rust-colored powdery substance, eventually leading to leaf withering and death. Wheat stripe rust is one of the most important wheat diseases worldwide and a significant biological disaster affecting safe wheat production in China. In China, it mainly occurs in Hebei, Henan, Shandong, Shanxi, and Shaanxi provinces.

[0003] In field disease control practices, to achieve rapid diagnosis of wheat stripe rust, a stripe rust resistance gene detection kit is generally used for on-site screening. First, 5-10 representative plants are selected from each wheat field to be tested, and newly infected leaves are collected. One to two typical diseased leaves are collected from each plant as samples, and the diseased leaves are cut into pieces of about 1 cm. 2 After processing the small pieces, place them in the extraction tube provided with the kit and inject buffer. Due to the limitations of field operation conditions, it is difficult to use professional grinding equipment. At this time, the operator can manually grind the leaves with a grinding rod or directly squeeze the leaves with their fingers to break the cells and release the nucleic acid into the buffer. Let it stand for 1-2 minutes to wait for extraction. Then, use a pipette to draw an appropriate amount of extraction liquid and add it to the sample well of the test card. Let it stand for 5-10 minutes and observe the results. Through the rapid screening process, it is possible to efficiently identify individual plants or groups carrying disease resistance genes from a large amount of wheat germplasm resources (including varieties, lines and hybrid offspring) in the field environment. This provides important preliminary data support for subsequent disease resistance variety breeding, gene mapping research and molecular breeding work, which helps to improve the efficiency of wheat disease resistance breeding and reduce the risk of stripe rust.

[0004] However, existing technologies have the following problems in practical use;

[0005] When existing reagent kits are used in the field, operators need to manually or with a grinding rod break the leaves to break the cells inside and release the nucleic acid into the buffer solution. Manual processing is time-consuming and laborious, and it is difficult to break the cells completely. In addition, the field working environment is complex, and impurities such as soil and weed debris can easily mix into the ground sample, thus interfering with the accuracy of the test results. Utility Model Content

[0006] (a) Technical problems to be solved

[0007] To overcome the aforementioned deficiencies of the prior art, this utility model provides a stripe rust resistance gene detection kit, which solves the problem mentioned in the background art that when using existing kits in the field, operators need to manually or use a grinding rod to break the leaves to break the cells inside and fully release the nucleic acid into the buffer solution. Manual processing is time-consuming and laborious, and it is difficult to fully break the cells. In addition, the field working environment is complex, and impurities such as soil and weed debris can easily mix into the ground sample, thereby interfering with the accuracy of the detection results.

[0008] (II) Technical Solution

[0009] To achieve the above objectives, this utility model is implemented through the following technical solution: a stripe rust resistance gene detection kit, comprising a kit, wherein the upper end of the kit is provided with a crushing trough, a storage trough and a protective trough sequentially from left to right; a crushing barrel is provided on the inner wall of the crushing trough; a cutting head is provided on the inner bottom wall of the crushing barrel; a micro motor is detachably connected to the lower end of the crushing barrel; the output end of the micro motor passes through the bottom end of the crushing barrel and is engaged with the inner wall of the cutting head; a support base is detachably connected to the outer surface of the micro motor; the upper surface of the support base is detachably connected to the lower surface of the crushing barrel; and a sealing cap is threadedly connected to the upper end of the crushing barrel.

[0010] Preferably, the upper surface of the reagent kit is connected to a lid via a hinge, and a handle is detachably connected to one side of the lid.

[0011] Preferably, the inner wall of the storage tank is provided with a detection card, and the upper end of the detection card has a sample hole.

[0012] Preferably, a battery pack is provided on the inner bottom wall of the crushing trough, the output end of the battery pack is connected to the input end wire of the micro motor, the upper surface of the battery pack is detachably connected to the lower surface of the support base, and a control button is provided on the inner side wall of the crushing trough, the input end of the control button is connected to the output end wire of the micro motor.

[0013] Preferably, the inner wall of the crushing trough is provided with a plurality of sliding grooves, and the inner wall of each of the plurality of sliding grooves is slidably connected to a slider. A connecting rod is fixedly connected to the other side of the slider, and the other end of the connecting rod is fixedly connected to the outer surface of the crushing barrel.

[0014] Preferably, the inner wall of the protective groove is provided with a plurality of buffer components, which are arranged in a ring array around the vertical axis of the protective groove on the inner wall of the protective groove, and the other end of the buffer components is detachably connected to a limit ring.

[0015] Preferably, the inner wall of the limiting ring is fitted with a placement plate, the upper end of the placement plate is provided with multiple positioning holes, the multiple positioning holes are arranged in a circular array around the center point of the placement plate, and the lower end of the placement plate is detachably connected to a sponge base.

[0016] Preferably, the buffer assembly includes a damping rod, a return spring is sleeved on the outer surface of the damping rod, one end of the damping rod is detachably connected to the inner wall of the limiting ring, and the other end of the damping rod is detachably connected to the inner side wall of the protective groove.

[0017] (III) Beneficial Effects

[0018] This invention provides a stripe rust resistance gene detection kit, which has the following beneficial effects:

[0019] This stripe rust resistance gene detection kit, through the coordinated design of a crushing barrel, a cutting head, and a micro-motor, allows operators to place leaf samples inside the crushing barrel when needed. The micro-motor then drives the cutting head to rotate, rapidly crushing the leaf sample. This quickly and thoroughly breaks down the leaf tissue, enabling cells to rapidly release target substances such as nucleic acids. This avoids the problem of manual crushing, which often results in insufficient cell disruption. Furthermore, it eliminates the need for additional grinding and crushing tools, allowing for sample processing by a single person. This reduces the workload for operators and avoids inconsistent crushing results due to individual differences in skill. The sealed cap and crushing barrel ensure that the leaf sample is crushed in a sealed environment, preventing sample splashing and completely isolating it from field soil, weeds, and other impurities, reducing the risk of contamination and further improving the accuracy of the test results. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the crushing barrel structure of this utility model;

[0022] Figure 3 This is a schematic diagram of the limiting ring structure of this utility model;

[0023] Figure 4 This utility model Figure 3 Enlarged structural diagram at point A in the middle;

[0024] Figure 5 This is a schematic diagram of the battery pack structure of this utility model.

[0025] In the diagram: 1. Reagent kit; 2. Crushing tank; 3. Storage tank; 4. Protective tank; 5. Crushing bucket; 6. Cutting head; 7. Micro motor; 8. Support base; 9. Sealing cap; 10. Box lid; 11. Handle; 12. Test card; 13. Sample hole; 14. Battery pack; 15. Slide groove; 16. Slider; 17. Connecting rod; 18. Buffer assembly; 1801. Damping rod; 1802. Return spring; 19. Limiting ring; 20. Placement tray; 21. Positioning hole; 22. Sponge base. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0027] Example 1;

[0028] Please see Figure 1 and Figure 2 This utility model provides a technical solution: a stripe rust resistance gene detection kit, which is mainly used for wheat stripe rust detection. The kit includes a kit 1, with a lid 10 hinged to the upper surface of the kit 1. A handle 11 is detachably connected to one side of the lid 10. From left to right, the upper end of the kit 1 has a crushing trough 2, a storage trough 3, and a protective trough 4. A crushing barrel 5 is installed on the inner wall of the crushing trough 2, and a cutting head 6 is installed on the inner bottom wall of the crushing barrel 5. A micro motor 7 is detachably connected to the lower end of the crushing barrel 5. The output end of the micro motor 7 passes through the bottom end of the crushing barrel 5 and is engaged with the inner wall of the cutting head 6. The outer surface of the micro motor 7 is detachably connected to... The crushing tank 5 has a support base 8, the upper surface of which is detachably connected to the lower surface of the crushing tank 5. The upper end of the crushing tank 5 is threaded with a sealing cap 9. The inner bottom wall of the crushing trough 2 is provided with a battery pack 14. The output end of the battery pack 14 is connected to the input end wire of the micro motor 7. The upper surface of the battery pack 14 is detachably connected to the lower surface of the support base 8. The inner side wall of the crushing trough 2 is provided with a control button. The input end of the control button is connected to the output end wire of the micro motor 7. The inner side wall of the crushing trough 2 is evenly provided with multiple sliding grooves 15. The inner walls of the multiple sliding grooves 15 can be slidably connected with sliders 16. The other side of the sliders 16 is fixedly connected with a connecting rod 17. The other end of the connecting rod 17 is fixedly connected to the outer surface of the crushing tank 5.

[0029] Through the above technical solution, when processing wheat leaf samples, the battery pack 14 can provide power to the micro motor 7. The operator can activate the micro motor 7 by pressing the control button, thereby driving the cutting head 6 to cut the leaf samples inside the crushing barrel 5. The support base 8 fixes the position of the micro motor 7 and connects the micro motor 7 to the crushing barrel 5 to ensure the stability of the micro motor 7 during operation and prevent the micro motor 7 from shaking and affecting the crushing effect. The slider 16 and connecting rod 17 on the side of the crushing barrel 5 can lock the crushing barrel 5 into the groove 15 on the inner wall of the crushing trough 2. In this way, the stability of the crushing barrel 5 during the cutting and crushing process is ensured. After the crushing is completed, the cooperation between the slide groove 15 and the slider 16 allows the crushing barrel 5 to slide up and down in the crushing groove 2, which is convenient for the operator to install and remove the crushing barrel 5. At the same time, the connecting rod 17 connects the slider 16 and the crushing barrel 5 to ensure the stability and synchronization of the crushing barrel 5 during the sliding. After the operation is completed, the box cover 10 is closed to seal the reagent kit 1 and protect the internal components. When not in use, it prevents dust, dirt and other impurities from entering the reagent kit 1 and affecting the detection accuracy. The handle 11 on the side of the box cover 10 makes it convenient for the operator to hold.

[0030] Example 2;

[0031] Please see Figure 3 , Figure 4 and Figure 5 This utility model provides a technical solution based on Embodiment 1. The inner wall of the storage tank 3 is provided with a detection card 12, and the upper end of the detection card 12 is provided with a sample hole 13. The inner side wall of the protective tank 4 is provided with multiple buffer components 18. The multiple buffer components 18 are arranged in a ring around the vertical axis of the protective tank 4 on the inner side wall of the protective tank 4. The other end of the buffer component 18 is detachably connected to a limiting ring 19. The inner wall of the limiting ring 19 is clamped with a placement plate 20. The upper end of the placement plate 20 is provided with multiple positioning holes 21. The multiple positioning holes 21 are arranged in a ring around the center point of the placement plate 20. The lower end of the placement plate 20 is detachably connected to a sponge base 22. The buffer component 18 includes a damping rod 1801. The outer surface of the damping rod 1801 is sleeved with a return spring 1802. One end of the damping rod 1801 is detachably connected to the inner wall of the limiting ring 19, and the other end of the damping rod 1801 is detachably connected to the inner side wall of the protective tank 4.

[0032] Through the above technical solution, the positioning holes 21 on the surface of the placement tray 20 can accurately engage small containers such as sample tubes and aspirators during use. The circular array of holes forms a physical limit, ensuring that the containers remain vertical within the protective groove 4, preventing sample spillage due to shaking during transportation or operation. Meanwhile, the sponge base 22 at the bottom of the placement tray 20 is made of high-density cushioning material. On the one hand, it increases the coefficient of friction to prevent the placement tray 20 from sliding within the protective groove 4; on the other hand, it absorbs bottom vibrations through elastic deformation, providing secondary protection for the placed containers. This is particularly suitable for field transportation. During transport, the limiting ring 19 fixes the position of the placement tray 20 through the slot, forming multiple layers of protection with the surrounding buffer components 18. When the reagent kit 1 is subjected to external impact, the damping rod 1801 in the buffer component 18 quickly absorbs the lateral vibration through the hydraulic damping effect. After absorption, the return spring 1802 on its outer surface generates a reverse elastic force, causing the damping rod 1801 to reset. The broken leaf sample is then sucked out through the suction tube or other container in the placement tray 20 and sent into the sample hole 13 at the top of the detection card 12 for detection.

[0033] All electrical components mentioned in this article are connected to an external main controller and 220V AC mains power via standard interfaces. The main controller can be any commercially available known device. There are no special restrictions on the specific models of the electrical components; any commercially available ordinary products can be selected, as long as they meet the usage requirements of this utility model.

[0034] In this invention, the working steps of the device are as follows:

[0035] First, staff collect typical diseased leaf samples. Then, holding the handle 11 on the side of the box lid 10, they open the lid 10, revealing the internal crushing chamber 2, storage chamber 3, and protective chamber 4. They open the sealing cap 9 at the top of the crushing chamber 5, place the shredded leaf sample into the crushing chamber 5, add an appropriate amount of buffer solution, and tighten the sealing cap 9 to form a closed space. Pressing the control button on the inner wall of the crushing chamber 2 activates the battery pack 14, which powers the micro-motor 7, driving the cutting head 6 to rotate at high speed to cut and crush the leaf sample for 30 seconds to 1 minute. This allows for the efficient release of intracellular nucleic acid substances and rapid fusion with the buffer solution. Then, they place the sample on the placement tray 2. The sample solution is drawn up by the suction tube in the positioning hole 21 on the upper surface of the sample 0. Then, the test card 12 is taken out from the storage tank 3. After confirming that the sample hole 13 is not blocked, 3-4 drops of sample solution are added to the sample hole 13 and tested through the test card 12. By observing the color development on the test card 12, it is quickly determined whether there is a stripe rust resistance gene in the sample. At the same time, when the reagent kit 1 is subjected to vibration or impact during transportation, the damping rod 1801 can absorb the lateral vibration. After absorption, the reset spring 1802 quickly resets it, further protecting the sample and container in the placement tray 20 and reducing the risk of sample damage during transportation.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A test kit for detecting a stem rust resistance gene comprising a kit (1) characterized in that: The reagent kit (1) has a crushing tank (2), a storage tank (3) and a protective tank (4) arranged sequentially from left to right at its upper end. The inner wall of the crushing tank (2) is provided with a crushing barrel (5). The inner bottom wall of the crushing barrel (5) is provided with a cutting head (6). The lower end of the crushing barrel (5) is detachably connected to a micro motor (7). The output end of the micro motor (7) passes through the bottom end of the crushing barrel (5) and is clamped to the inner wall of the cutting head (6). The outer surface of the micro motor (7) is detachably connected to a support base (8). The upper surface of the support base (8) is detachably connected to the lower surface of the crushing barrel (5). The upper end of the crushing barrel (5) is threaded with a sealing cap (9).

2. The kit for detecting a stem rust resistance gene according to claim 1, characterized in that: The upper surface of the reagent kit (1) is connected to a lid (10) by a hinge, and a handle (11) is detachably connected to one side of the lid (10).

3. The kit for detecting a stem rust resistance gene according to claim 1, characterized in that: The inner wall of the storage tank (3) is provided with a detection card (12), and the upper end of the detection card (12) is provided with a sample hole (13).

4. The kit for detecting a stem rust resistance gene according to claim 1, characterized in that: The inner bottom wall of the crushing trough (2) is provided with a battery pack (14), the output end of the battery pack (14) is connected to the input end wire of the micro motor (7), the upper surface of the battery pack (14) is detachably connected to the lower surface of the support base (8), and the inner side wall of the crushing trough (2) is provided with a control button, the input end of the control button is connected to the output end wire of the micro motor (7).

5. The kit for detecting a stem rust resistance gene according to claim 1, characterized in that: The inner wall of the crushing trough (2) is evenly provided with multiple sliding grooves (15), and the inner wall of each of the multiple sliding grooves (15) can be slidably connected with a slider (16). The other side of the slider (16) is fixedly connected with a connecting rod (17), and the other end of the connecting rod (17) is fixedly connected to the outer surface of the crushing barrel (5).

6. The kit for detecting a stem rust resistance gene according to claim 1, characterized in that: The inner wall of the protective groove (4) is provided with a plurality of buffer components (18), which are arranged in a ring array around the vertical axis of the protective groove (4) on the inner wall of the protective groove (4). The other end of the buffer component (18) is detachably connected to a limit ring (19).

7. The kit for detecting a stem rust resistance gene according to claim 6, characterized in that: The inner wall of the limiting ring (19) is fitted with a placement plate (20). The upper end of the placement plate (20) is provided with multiple positioning holes (21). The multiple positioning holes (21) are arranged in a circular array around the center point of the placement plate (20). The lower end of the placement plate (20) is detachably connected to a sponge base (22).

8. The stripe rust resistance gene detection kit according to claim 6, characterized in that: The buffer assembly (18) includes a damping rod (1801), and a return spring (1802) is sleeved on the outer surface of the damping rod (1801). One end of the damping rod (1801) is detachably connected to the inner wall of the limiting ring (19), and the other end of the damping rod (1801) is detachably connected to the inner side wall of the protective groove (4).

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