An immune cell isolation device

Abstract

The utility model relates to cell separation device technical field discloses an immune cell separation device, including the case, the top rear side of case rotatory connection has the apron, the inside bottom fixed connection of case has brushless motor, the output fixed connection of brushless motor has rotor, the inside middle part fixed connection of case has magnetic suspension bearing, the inside fixed connection of magnetic suspension bearing has a plurality of electromagnetic blocks, the inside sliding connection of magnetic suspension bearing has a plurality of arc support plate, the outer wall fixed connection of arc support plate has butterfly spring. In the utility model, through starting brushless motor, makes rotor and conical rotary disc rotate, sample test tube is placed in the placement hole of rotary disc, rotates along with conical rotary disc, magnetic suspension bearing generates magnetic field through electromagnetic block, and cooperates with rotor, and arc support plate slides in bearing, and butterfly spring provides buffer shock attenuation, avoids the influence of vibration and separates effect.

Description

Technical Field

[0001] This utility model relates to the field of cell separation device technology, and in particular to an immune cell separation device. Background Technology

[0002] Immune cell separation devices are key equipment in the biomedical field used to accurately extract specific immune cells from complex biological samples. They play an irreplaceable role in disease diagnosis, cell therapy, and scientific research experiments. Their core function is to use the physical or biological properties of cells to separate target immune cells from blood and tissue fluid samples, providing high-purity, high-activity cell samples for subsequent research and clinical applications.

[0003] Early immune cell separation devices primarily used traditional mechanical bearings to support the centrifuge stand. During operation, friction between the bearing and the shaft was unavoidable. As separation continued for extended periods, the mechanical bearing generated significant heat due to friction. This heat was conducted into the centrifuge chamber, causing the sample temperature to rise. Immune cells are extremely sensitive to temperature; temperature fluctuations significantly reduced cell viability and could even lead to cell death, resulting in low cell viability retention after separation. Furthermore, the mechanical bearing vibrated and wore during high-speed rotation, affecting the stability of the centrifugation process, reducing cell separation purity, and requiring frequent bearing replacements, increasing equipment maintenance costs and downtime. Current devices incorporate magnetic levitation bearing technology. Magnetic levitation bearings use magnetic force to achieve non-contact support of the centrifuge stand, fundamentally eliminating mechanical friction and avoiding the impact of frictional heat on cell viability, thus improving cell viability retention. However, existing immune cell separation devices still suffer from uneven magnetic force distribution, causing slight radial displacement of the centrifuge stand during high-speed rotation. This disrupts the separation interface within the centrifuge chamber, affecting cell separation purity. Utility Model Content

[0004] To overcome the above deficiencies, this invention provides an immune cell separation device, which aims to improve the problem in the prior art where uneven magnetic force distribution causes slight radial displacement of the centrifuge support during high-speed rotation, resulting in disorder at the separation interface of the sample in the centrifuge chamber.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an immune cell separation device, comprising a chassis, a cover plate rotatably connected to the rear top of the chassis, a brushless motor fixedly connected to the bottom inside the chassis, a rotor fixedly connected to the output end of the brushless motor, a magnetic levitation bearing fixedly connected to the middle inside the chassis, multiple electromagnetic blocks fixedly connected inside the magnetic levitation bearing, multiple arc-shaped support plates slidably connected inside the magnetic levitation bearing, a butterfly spring fixedly connected to the outer wall of the arc-shaped support plate, a conical rotating disk fixedly connected to the top of the rotor, multiple placement holes opened inside the conical rotating disk, and a limiting mechanism provided on the outer wall of the conical rotating disk, the limiting mechanism being used to enhance the binding of magnetic beads to cells.

[0006] As a further description of the above technical solution:

[0007] The limiting mechanism includes a limiting ring, the outer wall of which is opened in the middle of the outer wall of the conical rotating disk. An electromagnetic ring is fixedly connected to the inner wall of the conical rotating disk. An annular magnetic shielding ring is fixedly connected to the outer wall of the limiting ring. A conical magnetic flux concentrator is fixedly connected to the middle of the top of the conical rotating disk. The top of the conical magnetic flux concentrator has multiple micro-guide holes.

[0008] As a further description of the above technical solution:

[0009] A display screen is fixedly connected to the front left side of the chassis, and multiple function buttons are fixedly connected to the front right side of the chassis.

[0010] As a further description of the above technical solution:

[0011] A power switch is fixedly connected to the right side of the chassis. The power switch is electrically connected to the brushless motor, the electromagnetic block, the display screen, and the function buttons.

[0012] As a further description of the above technical solution:

[0013] The bottom of the inner wall of the cover plate is provided with a conical notch, and the top of the chassis is provided with a limiting notch.

[0014] As a further description of the above technical solution:

[0015] The chassis has an inclined heat dissipation vent on the rear side, and multiple guide plates are fixedly connected to the inner wall of the inclined heat dissipation vent.

[0016] As a further description of the above technical solution:

[0017] The bottom of the chassis is threaded with a spiral support block at each of the four corners, and an arc-shaped buffer block is fixedly connected to the outer wall of the electromagnetic block.

[0018] As a further description of the above technical solution:

[0019] The outer wall dimension of the rotor is smaller than the inner wall dimension of the magnetic levitation bearing, and the inner wall dimension of the limiting ring is the same as the outer wall dimension of the electromagnetic ring.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, by starting the brushless motor, the rotor and the conical rotating disk are rotated. The sample tube is placed in the placement hole of the rotating disk and rotates with the conical rotating disk. The magnetic levitation bearing generates a magnetic field through the electromagnetic block, which cooperates with the rotor to realize the magnetic levitation support of the rotor, ensuring contactless and low friction during high-speed rotation. The arc-shaped support plate slides in the bearing, and the butterfly spring provides buffering and shock absorption to ensure rotational stability and avoid vibration affecting the separation effect.

[0022] 2. In this invention, the magnetic field generated by the energized electromagnetic ring can attract and assist in the separation of specifically labeled cells. The annular magnetic shielding ring is used to shield stray magnetic fields to avoid interference. In addition, the conical magnetic flux concentrator can concentrate magnetic lines of force and form a spiral flow under the action of centrifugal force through micro-guide holes, thereby accelerating the cell separation process. Attached Figure Description

[0023] Figure 1 This is a perspective view of an immune cell separation device proposed in this utility model;

[0024] Figure 2 This is a front view of an immune cell separation device proposed in this utility model;

[0025] Figure 3 This is a cross-sectional view of an immune cell separation device proposed in this utility model;

[0026] Figure 4 This is a split view of the conical rotating disk of an immune cell separation device proposed in this utility model;

[0027] Figure 5 This is a schematic diagram of the structure of a magnetic levitation bearing for an immune cell separation device proposed in this utility model.

[0028] Legend:

[0029] 1. Chassis; 2. Limiting mechanism; 201. Limiting ring; 202. Electromagnetic ring; 203. Annular magnetic shielding ring; 204. Conical magnetic flux concentrator; 205. Miniature flow guide hole; 3. Cover plate; 4. Brushless motor; 5. Rotor; 6. Arc-shaped support plate; 7. Butterfly spring; 8. Electromagnetic block; 9. Magnetic levitation bearing; 10. Conical rotating disk; 11. Placement hole; 12. Display screen; 13. Function button; 14. Power switch; 15. Conical notch; 16. Limiting notch; 17. Inclined heat dissipation vent; 18. Spiral support block; 19. Arc-shaped buffer block; 20. Guide plate. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figure 1 , Figure 3 and Figure 5 This utility model provides an embodiment of an immune cell separation device, comprising a housing 1, a cover plate 3 rotatably connected to the rear top of the housing 1, a brushless motor 4 fixedly connected to the bottom inside the housing 1, a rotor 5 fixedly connected to the output end of the brushless motor 4, a magnetic levitation bearing 9 fixedly connected to the middle inside the housing 1, and multiple electromagnetic blocks 8 fixedly connected inside the magnetic levitation bearing 9. When energized, the electromagnetic blocks 8 inside the magnetic levitation bearing 9 generate a magnetic field, which, in conjunction with the rotor 5, levitates and supports the rotor 5 using magnetic force, allowing the rotor 5 and the conical rotating disk 10 to rotate at high speed without contact and with low friction. Multiple arc-shaped supports are slidably connected inside the magnetic levitation bearing 9. Plate 6, the outer wall of the arc-shaped support plate 6 is fixedly connected to a butterfly spring 7, the arc-shaped support plate 6 slides in the magnetic levitation bearing 9, the butterfly spring 7 plays the role of buffering and shock absorption, ensuring the stability of rotation, the top of the rotor 5 is fixedly connected to a conical rotating disk 10, the brushless motor 4 starts first, drives the rotor 5 to start rotating, the rotor 5 then drives the conical rotating disk 10 fixedly connected to it to rotate, the conical rotating disk 10 has multiple placement holes 11 inside, the placement holes 11 on the conical rotating disk 10 are used to load sample test tubes, the test tubes rotate together with the conical rotating disk 10, the outer wall of the conical rotating disk 10 is provided with a limit mechanism 2, the limit mechanism 2 is used to enhance the binding of magnetic beads and cells;

[0032] Specifically, when the immune cell separation device is started, the brushless motor 4 starts first, driving the rotor 5 to rotate. The rotor 5 then drives the conical rotating disk 10, which is fixedly connected to it, to rotate. The conical rotating disk 10 has holes 11 for loading sample tubes. The sample tubes rotate together with the conical rotating disk 10. During the rotation, the magnetic levitation bearing 9 plays a key role. When the electromagnetic block 8 inside is energized, it generates a magnetic field, which works with the rotor 5 to levitate and support the rotor 5 using magnetic force. This allows the rotor 5 and the conical rotating disk 10 to rotate at high speed without contact and with low friction. At the same time, the arc-shaped support plate 6 slides within the magnetic levitation bearing 9, and the butterfly spring 7 acts as a buffer and shock absorber to ensure the stability of the rotation and prevent vibration from affecting the sample separation effect.

[0033] Reference Figure 1 , Figure 3 and Figure 4 The limiting mechanism 2 includes a limiting ring 201. The outer wall of the limiting ring 201 is opened in the middle of the outer wall of the conical rotating disk 10. An electromagnetic ring 202 is fixedly connected to the inner wall of the conical rotating disk 10. When the electromagnetic ring 202 is energized, it generates a magnetic field, which exerts a magnetic force on the magnetic beads that are labeled with target cells in the sample tube, thus assisting in cell separation. An annular magnetic shielding ring 203 is fixedly connected to the outer wall of the limiting ring 201. The annular magnetic shielding ring 203 can shield the stray magnetic field generated by the electromagnetic ring 202, thus preventing interference with other components. A conical magnetic flux converging device 204 is fixedly connected to the middle of the top of the conical rotating disk 10. The top of the conical magnetic flux converging device 204 has multiple micro-guide holes 205. The conical magnetic flux converging device 204 converges the divergent magnetic lines generated by the electromagnetic ring 202. Through the micro-guide holes 205 at the top, under the action of centrifugal force, the liquid in the test tube forms a spiral flow, which accelerates the binding of the magnetic beads with the target cells.

[0034] Specifically, the electromagnetic ring 202 generates a magnetic field by being energized, which exerts a magnetic force on the magnetic beads labeled with target cells in the sample tube, assisting in cell separation. The annular magnetic shielding ring 203 can shield the stray magnetic field generated by the electromagnetic ring 202 to avoid interference with other components. The conical magnetic flux converging device 204 converges the divergent magnetic lines of force generated by the electromagnetic ring 202. Through the micro-guide hole 205 at the top, under the action of centrifugal force, the liquid in the test tube is made to form a spiral flow, which accelerates the binding of the magnetic beads with the target cells and further improves the separation efficiency.

[0035] Reference Figure 1 , Figure 2 and Figure 3A display screen 12 is fixedly connected to the front left side of the chassis 1. The display screen 12 is used to display the operating parameters of the equipment in real time. Multiple function buttons 13 are fixedly connected to the front right side of the chassis 1. The function buttons 13 can quickly switch the working mode of the equipment and set the operating parameters. A power switch 14 is fixedly connected to the right side of the chassis 1. The power switch 14 is electrically connected to the brushless motor 4, the electromagnetic block 8, the display screen 12 and the function buttons 13 respectively. The power switch 14 serves as the main control hub for the power supply of the entire equipment. A conical recess 15 is provided at the bottom of the inner wall of the cover plate 3. The conical recess 15 can be tightly fastened to the top of the conical rotating disk 10 to form a relatively closed centrifugal space. A limiting recess 16 is provided at the top of the chassis 1. The limiting recess 16 can ensure the stable connection between the cover plate 3 and the chassis 1 and prevent the cover plate 3 from shaking or shifting during the operation of the equipment.

[0036] Specifically, the display screen 12 is used to display the equipment operating parameters in real time, which makes it convenient for operators to intuitively monitor the equipment operation and adjust the separation program in a timely manner. The function button 13 can quickly switch the working mode of the equipment and set the operating parameters to achieve precise control of the immune cell separation process. The power switch 14 serves as the main control hub for the power supply of the entire equipment. When the cover plate 3 is closed, the conical notch 15 can be tightly fastened above the conical rotating disk 10 to form a relatively closed centrifugation space, reducing external interference. The limiting notch 16 can ensure the stable connection between the cover plate 3 and the chassis 1, preventing the cover plate 3 from shaking or shifting during equipment operation, and ensuring the safety and stability of centrifugation operation.

[0037] Reference Figure 1 , Figure 3 and Figure 4 The rear side of the chassis 1 is provided with an inclined heat dissipation vent 17, which facilitates the natural upward discharge of hot air. Multiple guide plates 20 are fixedly connected to the inner wall of the inclined heat dissipation vent 17, which can guide the hot air to be discharged more smoothly through the heat dissipation vent. The bottom of the chassis 1 has four corners with threaded spiral support blocks 18, which can be adjusted in height according to the actual situation of the placement plane. The outer wall of the electromagnetic block 8 is fixedly connected with an arc-shaped buffer block 19, which can absorb the small vibration and impact force generated by the change of magnetic force of the electromagnetic block 8. The outer wall size of the rotor 5 is smaller than the inner wall of the magnetic levitation bearing 9, realizing the non-contact rotation of the rotor 5, effectively reducing mechanical friction and wear. The inner wall size of the limiting ring 201 is the same as the outer wall size of the electromagnetic ring 202, so that the magnetic field generated by the electromagnetic ring 202 acts more concentrated on the sample tube, enhancing the magnetic adsorption effect on the magnetic beads.

[0038] Specifically, the inclined heat dissipation vent 17 facilitates the natural upward expulsion of hot air, while the guide plate 20 guides the hot air to pass more smoothly through the heat dissipation vent, accelerating the heat dissipation speed and preventing the performance and service life of the brushless motor 4 and electromagnetic block 8 from being affected by excessive temperature, ensuring the stable operation of the equipment for a long time. The spiral support block 18 can be height adjusted according to the actual situation of the placement plane to ensure that the equipment is placed stably. The arc-shaped buffer block 19 can absorb the small vibrations and impacts generated by the electromagnetic block 8 due to changes in magnetic force, preventing the vibration from being transmitted to the rotor 5 and the conical rotating disk 10. The outer wall size of the rotor 5 is smaller than the inner wall size of the magnetic levitation bearing 9, realizing the non-contact rotation of the rotor 5, effectively reducing mechanical friction and wear, reducing operating noise and heat generation, thereby protecting the activity of immune cells. The inner wall size of the limiting ring 201 is the same as the outer wall size of the electromagnetic ring 202, which allows the magnetic field generated by the electromagnetic ring 202 to act more concentrated on the sample tube, enhancing the magnetic adsorption effect on the magnetic beads and improving the separation purity of immune cells.

[0039] Working principle: First, during the use of this immune cell separation device, the brushless motor 4 starts first, which drives the rotor 5 to rotate. The rotor 5 then drives the conical rotating disk 10, which is fixedly connected to it, to rotate. The conical rotating disk 10 is provided with placement holes 11 for placing sample tubes. The test tubes rotate synchronously with the conical rotating disk 10. During the rotation, the magnetic levitation bearing 9 plays a key role. When the electromagnetic block 8 inside is energized, it generates a magnetic field, which works in conjunction with the rotor 5 to achieve the levitation support of the rotor 5 using magnetic force. This ensures that the rotor 5 and the conical rotating disk 10 can rotate at high speed in a non-contact, low-friction environment. At the same time, the arc-shaped support plate 6 slides inside the magnetic levitation bearing 9, and the butterfly spring 7 plays a role in buffering and shock absorption, ensuring the stability of the rotation process and avoiding the impact of vibration on the sample separation effect.

[0040] Furthermore, through the limiting mechanism 2 and the energization of the electromagnetic ring 202, a magnetic field is generated, which then applies a magnetic force to the magnetic beads labeled with target cells in the sample tube to assist in the cell separation process. The annular magnetic shielding ring 203 serves to shield the stray magnetic field generated by the electromagnetic ring 202, preventing it from interfering with other components. The conical magnetic flux converging device 204 is responsible for converging the divergent magnetic lines of force generated by the electromagnetic ring 202, and through the micro-guide hole 205 at the top, under the action of centrifugal force, it promotes the liquid in the test tube to form a spiral flow, thereby accelerating the binding of the magnetic beads with the target cells and further improving the separation efficiency.

[0041] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An immune cell isolation device comprising a housing (1), characterized in that: A cover plate (3) is rotatably connected to the rear top of the chassis (1). A brushless motor (4) is fixedly connected to the bottom inside the chassis (1). A rotor (5) is fixedly connected to the output end of the brushless motor (4). A magnetic levitation bearing (9) is fixedly connected to the middle inside the chassis (1). Multiple electromagnetic blocks (8) are fixedly connected inside the magnetic levitation bearing (9). Multiple arc-shaped support plates (6) are slidably connected inside the magnetic levitation bearing (9). A butterfly spring (7) is fixedly connected to the outer wall of the arc-shaped support plate (6). A conical rotating disk (10) is fixedly connected to the top of the rotor (5). Multiple placement holes (11) are opened inside the conical rotating disk (10). A limiting mechanism (2) is provided on the outer wall of the conical rotating disk (10). The limiting mechanism (2) is used to enhance the binding of the magnetic beads to the cells.

2. The immune cell isolation device of claim 1, wherein: The limiting mechanism (2) includes a limiting ring (201). The outer wall of the limiting ring (201) is opened in the middle of the outer wall of the conical rotating disk (10). An electromagnetic ring (202) is fixedly connected to the inner wall of the conical rotating disk (10). An annular magnetic shielding ring (203) is fixedly connected to the outer wall of the limiting ring (201). A conical magnetic flux concentrator (204) is fixedly connected to the middle of the top of the conical rotating disk (10). A plurality of micro-guide holes (205) are opened at the top of the conical magnetic flux concentrator (204).

3. The immune cell isolation device of claim 1, wherein: A display screen (12) is fixedly connected to the front left side of the chassis (1), and multiple function buttons (13) are fixedly connected to the front right side of the chassis (1).

4. The immune cell isolation device of claim 3, wherein: A power switch (14) is fixedly connected to the right side of the chassis (1). The power switch (14) is electrically connected to the brushless motor (4), the electromagnetic block (8), the display screen (12), and the function button (13).

5. The immune cell isolation device of claim 1, wherein: The bottom of the inner wall of the cover plate (3) is provided with a conical notch (15), and the top of the chassis (1) is provided with a limiting notch (16).

6. The immune cell isolation device of claim 1, wherein: The chassis (1) has an inclined heat dissipation vent (17) on the rear side, and multiple guide plates (20) are fixedly connected to the inner wall of the inclined heat dissipation vent (17).

7. The immune cell isolation device of claim 1, wherein: The bottom of the chassis (1) is threaded with a spiral support block (18) at each of the four corners, and the outer wall of the electromagnetic block (8) is fixedly connected with an arc-shaped buffer block (19).

8. The immune cell isolation device of claim 2, wherein: The outer wall size of the rotor (5) is smaller than the inner wall size of the magnetic levitation bearing (9), and the inner wall size of the limiting ring (201) is the same as the outer wall size of the electromagnetic ring (202).

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