Mesenchymal stem cell extraction device

Abstract

This utility model discloses a mesenchymal stem cell extraction device, belonging to the field of stem cell extraction technology. It includes an open-top mounting box, within which a centrifuge rotating seat is rotatably mounted. A first motor driving the centrifuge rotating seat is located at the bottom of the mounting box. Multiple centrifuge tubes are placed on the centrifuge rotating seat. A processing box is slidably mounted above the mounting box. A drive assembly driving the processing box to move up and down is located on one side of the mounting box. The processing box is internally divided into an upper processing chamber and a lower diversion chamber. This mesenchymal stem cell extraction device, through the drive assembly driving the processing box to move up and down, diverts the processed suspension in the processing chamber to multiple diversion nozzles and into multiple centrifuge tubes via the diversion chamber, thereby achieving simultaneous introduction of multiple centrifuge tubes. Compared to existing devices, this avoids the cumbersome problem of sequentially transferring and injecting multiple centrifuge tubes using a pipette, thus improving extraction efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of stem cell extraction technology, and in particular relates to a mesenchymal stem cell extraction device. Background Technology

[0002] Mesenchymal stem cells (MSCs) are pluripotent adult stem cells derived from the mesoderm that have the ability to self-renew. They can differentiate into various cell types, such as chondrocytes, adipocytes, osteoblasts, and other cell types, and are therefore used in many types of research.

[0003] In current mesenchymal stem cell extraction processes, taking adipose tissue extraction as an example, the adipose tissue needs to be collected, washed, and enzymatically digested before centrifugation to retain the underlying cell pellet. In practice, the processed stem cell extract needs to be manually transferred to centrifuge tubes using a pipette before centrifugation. This often requires multiple centrifuge tubes, and the sequential transfer and injection using a pipette is cumbersome and affects extraction efficiency.

[0004] To address this issue, we propose a mesenchymal stem cell extraction device. Utility Model Content

[0005] The purpose of this invention is to solve the problem of low extraction efficiency in the existing technology by proposing a mesenchymal stem cell extraction device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A mesenchymal stem cell extraction device includes an open-top mounting box, a centrifuge rotating seat rotatably mounted inside the mounting box, a first motor driving the centrifuge rotating seat to rotate at the lower part of the mounting box, multiple centrifuge tubes placed on the centrifuge rotating seat, a processing box sliding vertically above the mounting box, a driving assembly driving the processing box to move vertically on one side of the mounting box, the processing box being divided into an upper processing chamber and a lower diversion chamber, a valve assembly being provided between the processing chamber and the diversion chamber, and multiple diversion nozzles corresponding one-to-one with the centrifuge tubes being fixedly connected to the bottom of the processing box, and the diversion nozzles communicating with the diversion chamber.

[0008] Preferably, a centrifuge cap is fixedly connected to one side of the centrifuge tube by a soft material, and a pressure block is fixedly connected to the center of the bottom surface of the processing box.

[0009] Preferably, the flow-dividing cavity includes an upper flow-guiding cavity and a plurality of lower flow-guiding tubes, the top end of the flow-guiding tubes being connected to the flow-guiding cavity and the bottom end of the flow-guiding tubes being connected to the flow-dividing nozzle.

[0010] Preferably, the sum of the cross-sectional areas of the plurality of guide tubes is less than the cross-sectional area of ​​the guide cavity.

[0011] Preferably, a column is fixedly connected to one side of the mounting box, and a moving groove is opened on the side of the column facing the processing box. The driving assembly includes a lead screw rotatably disposed in the moving groove. A moving block is fixedly connected to one side of the processing box, and the moving block is threaded onto the lead screw. A second motor for driving the lead screw to rotate is fixedly installed at one end of the column.

[0012] Preferably, when the top of the mounting box is flush with the bottom of the processing box, the bottom of the diverter nozzle is higher than the top of the centrifuge tube.

[0013] In summary, the technical effects and advantages of this utility model are as follows: This mesenchymal stem cell extraction device drives the processing box to move up and down through the driving component, and diverts the processed suspension in the processing chamber to multiple diversion nozzles and into multiple centrifuge tubes through the diversion chamber, thereby realizing the synchronous introduction of multiple centrifuge tubes. Compared with the existing device, it avoids the problem of needing to use a pipette to transfer and inject multiple centrifuge tubes one by one, which is quite troublesome, and improves the extraction efficiency. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a cross-sectional front view of the present invention;

[0016] Figure 3 This is a cross-sectional side view of the present invention when it is injected into a centrifuge tube;

[0017] Figure 4 This is a top cross-sectional view of the present invention;

[0018] Figure 5 This is a cross-sectional side view of the present invention during centrifugation.

[0019] In the diagram: 1. Mounting box; 2. Centrifuge rotating seat; 3. First motor; 4. Centrifuge tube; 5. Processing box; 6. Processing chamber; 7. Diverting chamber; 8. Valve assembly; 9. Diverting nozzle; 10. Centrifuge cover; 11. Pressing block; 12. Guide chamber; 13. Guide pipe; 14. Column; 15. Moving groove; 16. Lead screw; 17. Moving block; 18. Second motor. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] Reference Figure 1-3A mesenchymal stem cell extraction device includes an open-top mounting box 1, a centrifugal rotating seat 2 rotatably mounted inside the mounting box 1, a first motor 3 driving the centrifugal rotating seat 2 to rotate at the lower part of the mounting box 1, multiple centrifuge tubes 4 placed on the centrifugal rotating seat 2, a processing box 5 sliding up and down above the mounting box 1, a driving component driving the processing box 5 to move up and down on one side of the mounting box 1, the processing box 5 is divided into an upper processing chamber 6 and a lower diversion chamber 7, a valve assembly 8 is provided between the processing chamber 6 and the diversion chamber 7, and multiple diversion nozzles 9 corresponding one-to-one with the centrifuge tubes 4 are fixedly connected to the bottom of the processing box 5, and the diversion nozzles 9 are connected to the diversion chamber 7.

[0022] In use, this mesenchymal stem cell extraction device first moves the processing box 5 upward via the drive assembly, inserting all the centrifuge tubes 4 into the centrifuge rotating seat 2. Afterward, the mounting box 1 is moved downward via the drive assembly until the bottom of the dispensing nozzle 9 is inserted into the centrifuge tube 4. Figure 3 At this point, tissues containing mesenchymal stem cells (such as adipose tissue, bone marrow tissue, or umbilical cord tissue) can be collected and enzymatically digested in processing chamber 6. After completion, valve assembly 8 is opened, and the processing solution moves to the splitting chamber 7 and flows into multiple centrifuge tubes 4 through multiple splitting nozzles 9. After completion, the mounting box 1 is moved upwards by the drive assembly, and the centrifuge tubes 4 are manually covered. Then, the mounting box 1 is moved downwards by the drive assembly until it completely covers the open top surface of processing box 5. Figure 5 The centrifuge rotor 2 is driven by the first motor 3 to rotate, starting the centrifugation of the liquid in the centrifuge tube 4. After centrifugation, the processing box 5 is driven upward by the drive assembly, and the centrifuge tube 4 is manually removed from the centrifuge rotor 2, thus completing the use of the mesenchymal stem cell extraction device.

[0023] Reference Figure 2-4 A centrifuge tube 4 has a centrifuge cap 10 fixedly connected to one side by a soft material, and a pressure block 11 is fixedly connected to the center of the bottom surface of the processing box 5. The centrifuge tube 4 and the centrifuge cap 10 are existing technologies and will not be described in detail. The pressure block 11 is a soft sponge block, which is used to press down the centrifuge cap 10 when the centrifuge tube 4 is being filled, preventing the centrifuge cap 10 from rotating and affecting the filling of the centrifuge tube 4, thus improving stability.

[0024] The diversion chamber 7 includes an upper guide chamber 12 and multiple lower guide pipes 13. The top end of the guide pipes 13 is connected to the guide chamber 12, and the bottom end of the guide pipes 13 is connected to the diversion nozzle 9. After the valve assembly 8 is opened, the treatment fluid in the treatment chamber 6 enters the guide chamber 12, and then enters the multiple guide pipes 13 for diversion, thereby realizing the diversion of the treatment fluid to the diversion nozzle 9 for outflow.

[0025] The sum of the cross-sectional areas of the multiple guide tubes 13 is less than the cross-sectional area of ​​the guide cavity 12. This ensures that when the guide tubes 13 are guiding the flow, the liquid level in the guide cavity 12 is always higher than the bottom surface of the guide cavity 12, which improves the stability of the flow distribution and avoids the problem of uneven flow distribution.

[0026] Reference Figure 1-3 A column 14 is fixedly connected to one side of the mounting box 1, and a moving groove 15 is opened on the side of the column 14 facing the processing box 5. The driving assembly includes a lead screw 16 rotatably disposed in the moving groove 15. A moving block 17 is fixedly connected to one side of the processing box 5, and the moving block 17 is threaded onto the lead screw 16. A second motor 18 that drives the lead screw 16 to rotate is fixedly installed at one end of the column 14. This driving assembly drives the lead screw 16 to rotate through the second motor 18, thereby realizing the up and down movement of the moving block 17, and thus realizing the up and down movement adjustment of the processing box 5.

[0027] Reference Figure 5 When the top of the mounting box 1 is flush with the bottom of the processing box 5, the bottom of the diverter 9 is higher than the top of the centrifuge tube 4. This allows the processing box 5 to act as the top cover of the mounting box 1 during high-speed centrifugation, preventing external interference from causing danger. At this time, the bottom of the diverter 9 and the bottom of the pressure block 11 should both be higher than the top of the centrifuge tube 4 to prevent the diverter 9 and the pressure block 11 from affecting the rotation of the centrifuge tube 4.

[0028] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A mesenchymal stem cell extraction device comprising an open-top installation box (1), characterized in that, The mounting box (1) is equipped with a centrifugal rotating seat (2) that rotates. The mounting box (1) is equipped with a first motor (3) that drives the centrifugal rotating seat (2) to rotate. Multiple centrifugal tubes (4) are placed on the centrifugal rotating seat (2). A processing box (5) is slidably mounted on the top of the mounting box (1). A driving component that drives the processing box (5) to move up and down is provided on one side of the mounting box (1). The processing box (5) is divided into a processing chamber (6) located at the top and a diversion chamber (7) located at the bottom. A valve assembly (8) is provided between the processing chamber (6) and the diversion chamber (7). Multiple diversion nozzles (9) that correspond one-to-one with the centrifugal tubes (4) are fixedly connected to the bottom of the processing box (5), and the diversion nozzles (9) are connected to the diversion chamber (7).

2. The mesenchymal stem cell extraction device of claim 1, wherein, The centrifuge tube (4) is fixedly connected to a centrifuge cover (10) by a soft material on one side, and a pressure block (11) is fixedly connected to the center of the bottom surface of the processing box (5).

3. The mesenchymal stem cell extraction device of claim 1, wherein, The diversion cavity (7) includes an upper diversion cavity (12) and a plurality of lower diversion tubes (13), the top end of the diversion tubes (13) being connected to the diversion cavity (12) and the bottom end of the diversion tubes (13) being connected to the diversion nozzle (9).

4. The mesenchymal stem cell extraction device of claim 3, wherein, The sum of the cross-sectional areas of the plurality of the guide tubes (13) is less than the cross-sectional area of ​​the guide cavity (12).

5. The mesenchymal stem cell extraction device of claim 1, wherein, A column (14) is fixedly connected to one side of the mounting box (1), and a moving groove (15) is provided on the side of the column (14) facing the processing box (5). The driving assembly includes a lead screw (16) rotatably disposed in the moving groove (15). A moving block (17) is fixedly connected to one side of the processing box (5), and the moving block (17) is threaded onto the lead screw (16). A second motor (18) for driving the lead screw (16) to rotate is fixedly installed at one end of the column (14).

6. The mesenchymal stem cell extraction device of claim 1, wherein, When the top of the mounting box (1) is flush with the bottom of the processing box (5), the bottom of the diverter (9) is higher than the top of the centrifuge tube (4).

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