A tissue culture medium filling machine

Abstract

The utility model relates to plant culture technical field, concretely is a kind of tissue culture medium filling machine, including workbench, liquid storage cavity and filling mechanism, the liquid storage cavity and filling mechanism are all fixedly connected in the top of workbench, the inside of liquid storage cavity is provided with stirring mechanism, the surface of stirring mechanism is fixedly connected with cleaning assembly, and the liquid storage cavity and filling mechanism are fixedly connected through output pipe between them.The utility model, by setting cleaning assembly on the surface of stirring mechanism, the medium left on inner wall is scraped clean, without manual disassembly cleaning, effectively avoid medium to attach and breed bacteria, ensure that liquid storage cavity inside is clean, by setting the structure of telescopic spring and fixed half-arc plate on fixed base, this elastic fixing mode can adapt to different specifications of culture cup, and in filling process, can effectively buffer external force impact, prevent culture cup from shaking, deviation, ensure that medium filling amount is accurate, improve filling quality.

Description

Technical Field

[0001] This utility model relates to the field of plant culture technology, and in particular to a tissue culture medium filling machine. Background Technology

[0002] In plant tissue culture, the filling of culture medium is a crucial and fundamental step. With the continuous expansion of the application scope and scale of tissue culture technology, the demand for culture medium filling machines is also increasing. Large-scale tissue culture production, such as some large plant tissue culture bases and factories, can produce millions or even tens of millions of plants annually. This requires filling machines with high-efficiency filling capabilities to meet the demands of rapid, high-volume production and improve overall production efficiency.

[0003] Existing technologies, such as the utility model patent with publication number CN216549579U, disclose a culture medium filling machine, including a mixing tank with a stirring device inside. The mixing tank has a feed inlet with a cover at the top and a discharge outlet on the lower side of one side, with a solenoid valve on the discharge outlet. A dispensing plate with a hollow dispensing chamber is located on one side of the bottom of the mixing tank, and the dispensing chamber and discharge outlet are connected by a connecting pipe. A base is located directly below the dispensing plate, and a support plate is located between the dispensing plate and the base. A slide rail is located on the support plate, and a lifting plate is located between the two slide rails. Cylinders are attached to both ends of the bottom of the dispensing plate, and a filling head is located on the lifting plate. A groove is formed on the upper surface of the base, and a detachable mounting seat is located within the groove. A placement groove is formed on the upper surface of the mounting seat, and a culture bottle is placed in the placement groove. This utility model has the advantages of high filling efficiency, easy disassembly and cleaning of the filling needle, strong applicability, and wide range of applications.

[0004] The above-mentioned technologies have certain drawbacks. First, the mixing tank lacks an effective internal wall cleaning mechanism. During long-term use, the culture medium easily adheres to the inner wall of the tank, making it difficult to clean. This not only affects the mixing effect but may also breed bacteria and contaminate subsequent culture media. Second, the culture bottles are fixed only by the placement slot. During the filling process, if they are subjected to slight shaking or impact from the filling head, the culture bottles are prone to shifting or even tipping over, resulting in inaccurate filling volume of culture medium, spillage, and waste of resources and environmental pollution. Utility Model Content

[0005] The purpose of this invention is to solve the problems of difficulty in cleaning the inner wall and insecure fixation of culture cups in the prior art, and to propose a tissue culture medium filling machine.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a tissue culture medium filling machine, comprising a workbench, a liquid storage chamber and a filling mechanism, wherein the liquid storage chamber and the filling mechanism are both fixedly connected to the top of the workbench, a stirring mechanism is provided inside the liquid storage chamber, a cleaning component is fixedly connected to the surface of the stirring mechanism, and the liquid storage chamber and the filling mechanism are fixedly connected through an output pipe.

[0007] The filling mechanism includes a fixed frame fixedly connected to the surface of the workbench, a lifting plate fixedly connected to the top inner part of the fixed frame, a sterilization transition box fixedly connected to the top of the fixed frame, a filling head body connected through the surface of the lifting plate, the sterilization transition box and the filling head body being fixedly connected by a connecting hose, a fixed base slidably connected to the bottom of the fixed frame, and a culture cup placed inside the fixed base.

[0008] Furthermore, the inner wall of the bottom of the fixed frame is provided with a sliding groove, and a fixed base is slidably connected inside the sliding groove. A fixed plate is fixedly connected to the upper surface of the fixed base, and a telescopic spring is fixedly connected to the surface of the fixed plate. A fixed semi-circular plate is fixedly connected to the end of the telescopic spring away from the fixed plate.

[0009] Furthermore, the fixing plates are symmetrically distributed on the upper surface of the fixing base, and the telescopic springs are distributed in several groups at linear intervals on the surface of the fixing plates.

[0010] Furthermore, the side of the fixed frame is provided with a groove, and the lifting plate is slidably connected to the groove. The lifting plate includes an electric push rod fixedly connected to the bottom of the disinfection transition box. The telescopic end of the electric push rod is fixedly connected to a connecting plate, and the filling head body is disposed inside the connecting plate.

[0011] Furthermore, the stirring mechanism includes a motor fixedly connected to the bottom of the liquid storage chamber, and a stirring rod fixedly connected to the output end of the motor, with stirring blades provided on the outer surface of the stirring rod.

[0012] Furthermore, the cleaning component includes a connecting ring fixedly connected to the outer surface of the stirring rod, and a scraper is fixedly connected to the outer surface of the connecting ring. The scraper has three sets that are spaced apart on the outer wall of the connecting ring.

[0013] Furthermore, a water pump is fixedly connected to the outer surface of the liquid storage chamber, the output end of the water pump is fixedly connected to one end of the output pipe, and a drain outlet is provided on the outer surface of the liquid storage chamber.

[0014] Furthermore, the top of the liquid storage chamber is provided with a liquid inlet pipe, and a sealing cap is threaded onto the outer surface of the liquid inlet pipe.

[0015] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0016] 1. In this utility model, by setting a cleaning component on the surface of the stirring mechanism, when the motor drives the stirring rod and stirring blade to stir the culture medium in the storage chamber, the scraper connected to the stirring rod will rotate synchronously with the stirring rod. The three sets of spaced scrapers can fully cover the inner wall of the storage chamber and scrape and clean the culture medium remaining on the inner wall without manual disassembly and cleaning. This effectively avoids the growth of bacteria on the culture medium, ensures the cleanliness of the inside of the storage chamber, and provides a good environment for the storage and mixing of the culture medium.

[0017] 2. In this utility model, by setting a telescopic spring and a fixed semi-circular plate on the fixed base, when the culture cup is placed on the fixed base, the fixed semi-circular plate is squeezed by the culture cup, and the telescopic spring contracts to generate elastic force. The symmetrically distributed fixed semi-circular plates firmly clamp the culture cup from both sides. This elastic fixing method can adapt to culture cups of different specifications, and during the filling process, it can effectively buffer external force impact, prevent the culture cup from shaking or shifting, ensure accurate filling of culture medium, avoid spillage, and improve filling quality and efficiency. Attached Figure Description

[0018] Figure 1 A three-dimensional structural diagram of a tissue culture medium filling machine is provided for this utility model;

[0019] Figure 2 This utility model provides a schematic diagram of the filling mechanism in a tissue culture medium filling machine;

[0020] Figure 3 This invention provides a cross-sectional structural diagram of the liquid storage chamber in a tissue culture medium filling machine;

[0021] Figure 4 This utility model provides a cross-sectional structural diagram of a tissue culture medium filling machine;

[0022] Figure 5 This utility model relates to a tissue culture medium filling machine. Figure 3 Enlarged view of point A;

[0023] Figure 6 This is a front view schematic diagram of a tissue culture medium filling machine according to the present invention.

[0024] Legend:

[0025] 1. Workbench; 2. Liquid storage chamber; 21. Stirring mechanism; 211. Motor; 212. Stirring rod; 213. Stirring blade; 22. Cleaning assembly; 221. Connecting ring; 222. Scraper; 23. Liquid inlet pipe; 24. Sealing cap; 3. Filling mechanism; 31. Fixing frame; 32. Lifting plate; 321. Electric push rod; 322. Connecting plate; 33. Sterilization transition box; 34. Filling head body; 35. Connecting hose; 36. Fixing base; 361. Fixing plate; 362. Telescopic spring; 363. Fixing semi-circular plate; 37. Culture cup; 4. Output pipe; 5. Water pump. Detailed Implementation

[0026] Please see Figure 1-6 This utility model provides a technical solution: a tissue culture medium filling machine, including a workbench 1, a liquid storage chamber 2 and a filling mechanism 3.

[0027] The specific setup and function of its liquid storage chamber 2 and filling mechanism 3 will be explained below.

[0028] In this embodiment: the liquid storage chamber 2 and the filling mechanism 3 are both fixedly connected to the top of the workbench 1. The liquid storage chamber 2 is equipped with a stirring mechanism 21. A cleaning component 22 is fixedly connected to the surface of the stirring mechanism 21. The liquid storage chamber 2 and the filling mechanism 3 are fixedly connected through an output pipe 4.

[0029] The filling mechanism 3 includes a fixed frame 31 fixedly connected to the upper surface of the workbench 1. A lifting plate 32 is fixedly connected to the top of the fixed frame 31. A sterilization transition box 33 is fixedly connected to the top of the fixed frame 31. A filling head body 34 is connected through the surface of the lifting plate 32. The sterilization transition box 33 and the filling head body 34 are fixedly connected by a connecting hose 35. A fixed base 36 is slidably connected to the bottom of the fixed frame 31. A culture cup 37 is placed inside the fixed base 36.

[0030] The effects achieved by the above components are as follows: the workbench 1 serves as a basic support structure, providing a stable installation platform for the liquid storage chamber 2 and the filling mechanism 3; the liquid storage chamber 2 is used to store culture medium, the stirring mechanism 21 can stir the culture medium to prevent sedimentation, the cleaning component 22 cleans the inner wall simultaneously, and the output pipe 4 realizes the transportation of culture medium from the liquid storage chamber 2 to the filling mechanism 3; in the filling mechanism 3, the fixed frame 31 provides an installation frame for other components, the lifting plate 32 can drive the filling head body 34 to rise and fall, the disinfection transition box 33 disinfects the filling head body 34, the connecting hose 35 ensures the transportation of culture medium between the disinfection transition box 33 and the filling head body 34, and the fixed base 36 is used to place and fix the culture cup 37.

[0031] Specifically, the inner wall of the bottom of the fixed frame 31 is provided with a sliding groove, and the inside of the sliding groove is slidably connected to the fixed base 36. The upper surface of the fixed base 36 is fixedly connected to the fixed plate 361, and the surface of the fixed plate 361 is fixedly connected to the telescopic spring 362. The end of the telescopic spring 362 away from the fixed plate 361 is fixedly connected to the fixed semi-arc plate 363.

[0032] The effects achieved by the above components are as follows: the sliding connection between the bottom groove of the fixed frame 31 and the fixed base 36 makes it convenient for the operator to push the fixed base 36 containing the culture cup 37 into or out of the fixed frame 31; the elastic fixing structure composed of the fixed plate 361, the telescopic spring 362 and the fixed semi-arc plate 363 can automatically adjust the clamping force according to the diameter of the culture cup 37, and firmly and elastically fix the culture cup 37 to prevent it from shaking or tipping over during the filling process.

[0033] Specifically, the fixing plates 361 are symmetrically distributed on the upper surface of the fixing base 36, and the telescopic springs 362 are distributed in several groups at linear intervals on the surface of the fixing plates 361.

[0034] The effect achieved by the above components is that the symmetrically distributed fixing plate 361, together with multiple sets of spaced telescopic springs 362 and fixing semi-arc plate 363, can uniformly clamp and fix the culture cup 37 from multiple directions, further enhancing the fixing effect, keeping the culture cup 37 stable during the filling process, and ensuring the accuracy of the filling volume.

[0035] Specifically, the side of the fixed frame 31 is provided with a groove, and the lifting plate 32 is slidably connected to the groove. The lifting plate 32 includes an electric push rod 321 fixedly connected to the bottom of the disinfection transition box 33. The telescopic end of the electric push rod 321 is fixedly connected to a connecting plate 322, and the filling head body 34 is located inside the connecting plate 322.

[0036] The effects achieved by the above components are as follows: the groove on the side of the fixed frame 31 is slidably connected to the lifting plate 32, providing guidance and support for the up and down movement of the lifting plate 32; the electric push rod 321 drives the connecting plate 322 and the filling head body 34 to rise and fall through extension and retraction, and can adjust the position of the filling head body 34 according to the height of the culture cup 37, so that the filling head body 34 can accurately extend into the culture cup 37 for filling. At the same time, when not filling, the filling head body 34 can be raised to facilitate operations such as replacing the culture cup 37.

[0037] Specifically, the stirring mechanism 21 includes a motor 211 fixedly connected to the bottom of the liquid storage chamber 2, a stirring rod 212 fixedly connected to the output end of the motor 211, and stirring blades 213 provided on the outer surface of the stirring rod 212.

[0038] The effect achieved by the above components is as follows: after the motor 211 starts, it drives the stirring rod 212 and stirring blade 213 to rotate, stirring the culture medium in the liquid storage chamber 2, so that the components of the culture medium are fully mixed and uniform, avoiding precipitation and stratification, ensuring that the concentration of the culture medium in the filling is consistent, and providing a high-quality culture environment for plant tissue culture.

[0039] Specifically, the cleaning component 22 includes a connecting ring 221 fixedly connected to the outer surface of the stirring rod 212. A scraper 222 is fixedly connected to the outer surface of the connecting ring 221, and three sets of scrapers 222 are spaced apart on the outer wall of the connecting ring 221.

[0040] The effect achieved by the above components is as follows: when the stirring rod 212 rotates and stirs the culture medium, the connecting ring 221 and the scraper 222 connected to the stirring rod 212 rotate synchronously. The three sets of spaced scrapers 222 can fully fit the inner wall of the liquid storage chamber 2, scrape off the culture medium attached to the inner wall, prevent the culture medium residue from breeding bacteria, realize the automatic cleaning of the inner wall of the liquid storage chamber 2, reduce the amount of manual cleaning work, and ensure the cleanliness of the inside of the liquid storage chamber 2.

[0041] Specifically, a water pump 5 is fixedly connected to the outer surface of the liquid storage chamber 2, and the output end of the water pump 5 is fixedly connected to one end of the output pipe 4. A drain outlet is provided on the outer surface of the liquid storage chamber 2.

[0042] The effects achieved by the above components are as follows: the water pump 5 transports the culture medium that is stirred evenly in the storage chamber 2 to the filling mechanism 3 through the output pipe 4, so as to realize the quantitative delivery of the culture medium; the sewage outlet is used to discharge the culture medium and cleaning sewage in the storage chamber 2, so as to facilitate the cleaning and maintenance of the storage chamber 2.

[0043] Specifically, the top of the liquid storage chamber 2 is provided with a liquid inlet pipe 23, and a sealing cap 24 is threadedly connected to the outer surface of the liquid inlet pipe 23.

[0044] The above components achieve the following effects: the inlet pipe 23 is used to add culture medium into the storage chamber 2, and the threaded sealing cap 24 can seal the inlet pipe 23 when no culture medium is added, preventing external dust, bacteria, etc. from entering the storage chamber 2 and ensuring the purity of the culture medium in the storage chamber 2.

[0045] Working principle: When in use, first open the sealing cap 24 of the liquid inlet pipe 23 at the top of the liquid storage chamber 2, and inject the culture medium into the liquid storage chamber 2 through the liquid inlet pipe 23. After the injection is completed, tighten the sealing cap 24. Start the motor 211. The motor 211 drives the stirring rod 212 and stirring blade 213 to rotate, stirring the culture medium. At the same time, the scraper 222 of the cleaning component 22 rotates synchronously to clean the inner wall of the liquid storage chamber 2.

[0046] Place the culture cup 37 on the fixed base 36. The fixed semi-circular plate 363 is compressed, causing the telescopic spring 362 to contract and firmly clamp the culture cup 37 from both sides. Then, push the fixed base 36 into the fixed frame 31 along the sliding groove at the bottom of the fixed frame 31. According to the height of the culture cup 37, start the electric push rod 321. The electric push rod 321 drives the connecting plate 322 and the filling head body 34 to descend, so that the filling head body 34 extends into the culture cup 37 to a suitable position. Start the water pump 5. The water pump 5 delivers the evenly stirred culture medium in the liquid storage chamber 2 to the filling head body 34 through the output pipe 4 and the connecting hose 35, and fills it into the culture cup 37. After filling, the electric push rod 321 drives the filling head body 34 to rise and reset, remove the fixed base 36 from the fixed frame 31, and take out the culture cup 37. If it is necessary to clean the liquid storage chamber 2, the residual culture medium and cleaning wastewater can be discharged through the drain outlet.

Claims

1. A tissue culture medium filling machine, comprising a workbench (1), a liquid storage chamber (2), and a filling mechanism (3), characterized in that: The liquid storage chamber (2) and the filling mechanism (3) are both fixedly connected to the top of the workbench (1). The liquid storage chamber (2) is equipped with a stirring mechanism (21). A cleaning component (22) is fixedly connected to the surface of the stirring mechanism (21). The liquid storage chamber (2) and the filling mechanism (3) are fixedly connected through an output pipe (4). The filling mechanism (3) includes a fixed frame (31) fixedly connected to the upper surface of the workbench (1), a lifting plate (32) fixedly connected to the inner top of the fixed frame (31), a disinfection transition box (33) fixedly connected to the top of the fixed frame (31), a filling head body (34) being connected through the surface of the lifting plate (32), the disinfection transition box (33) and the filling head body (34) being fixedly connected by a connecting hose (35), a fixed base (36) being slidably connected to the bottom of the fixed frame (31), and a culture cup (37) being placed inside the fixed base (36).

2. The tissue culture medium filling machine according to claim 1, characterized in that: The inner wall of the bottom of the fixed frame (31) is provided with a sliding groove, and a fixed base (36) is slidably connected inside the sliding groove. A fixed plate (361) is fixedly connected to the upper surface of the fixed base (36), and a telescopic spring (362) is fixedly connected to the surface of the fixed plate (361). A fixed semi-arc plate (363) is fixedly connected to the end of the telescopic spring (362) away from the fixed plate (361).

3. The tissue culture medium filling machine according to claim 2, characterized in that: The fixing plates (361) are symmetrically distributed on the upper surface of the fixing base (36), and the telescopic springs (362) are distributed in several groups at linear intervals on the surface of the fixing plates (361).

4. The tissue culture medium filling machine according to claim 1, characterized in that: The fixed frame (31) has a groove on its side, and the lifting plate (32) is slidably connected to the groove. The lifting plate (32) includes an electric push rod (321) fixedly connected to the bottom of the disinfection transition box (33). The telescopic end of the electric push rod (321) is fixedly connected to a connecting plate (322), and the filling head body (34) is located inside the connecting plate (322).

5. The tissue culture medium filling machine according to claim 1, characterized in that: The stirring mechanism (21) includes a motor (211) fixedly connected to the bottom of the liquid storage chamber (2), and a stirring rod (212) fixedly connected to the output end of the motor (211). The outer surface of the stirring rod (212) is provided with stirring blades (213).

6. The tissue culture medium filling machine according to claim 1, characterized in that: The cleaning component (22) includes a connecting ring (221) fixedly connected to the outer surface of the stirring rod (212). A scraper (222) is fixedly connected to the outer surface of the connecting ring (221). The scraper (222) has three sets of scrapers that are spaced apart on the outer wall of the connecting ring (221).

7. A tissue culture medium filling machine according to claim 1, characterized in that: A water pump (5) is fixedly connected to the outer surface of the liquid storage chamber (2). The output end of the water pump (5) is fixedly connected to one end of the output pipe (4). A drain outlet is provided on the outer surface of the liquid storage chamber (2).

8. A tissue culture medium filling machine according to claim 1, characterized in that: The top of the liquid storage chamber (2) is provided with a liquid inlet pipe (23), and a sealing cap (24) is threadedly connected to the outer surface of the liquid inlet pipe (23).

Images