A sampling device for stem cells

Abstract

The utility model discloses a kind of stem cell sampling devices, including sample-keeping outer tube, the inner tube structure is arranged on the sample-keeping outer tube, the sample-keeping outer tube upper end movable sleeve is equipped with buckle cap structure;The utility model relates to cell sampling device technical field, device uses sample-keeping outer tube and pipe body double-layer structure, when placing umbilical cord tissue sample, under the action of guiding assembly, head pipe stretches out the top end of sample-keeping outer tube, try to avoid umbilical cord tissue sample contact the outer wall of sample-keeping outer tube, improve the sterile quality of umbilical cord tissue sample, when handling umbilical cord tissue sample, tail cover and top cover are taken off, PBS is discharged from bottom pipe, isolation net is used to prevent umbilical cord tissue sample from bottom pipe discharge, subsequently limit rod and broken rubber septum are taken out from pipe body, while umbilical cord tissue sample remains in pipe body, and handle liquid is poured into pipe body to flush umbilical cord tissue sample, without needing to transfer umbilical cord tissue sample to other parts, reduce the risk of contamination of umbilical cord tissue sample.

Description

Technical Field

[0001] This utility model relates to the field of cell sampling device technology, specifically a stem cell sampling device. Background Technology

[0002] Umbilical cord mesenchymal stem cells (UC-MSCs) are a type of pluripotent stem cells extracted from Wharton's jelly or perivascular tissue of the newborn's umbilical cord. They possess self-renewal, multi-lineage differentiation, and immunomodulatory capabilities, and are widely used in regenerative medicine and disease treatment research. During umbilical cord sampling, after the fetus is delivered and the umbilical cord is cut, usually before or after placental delivery, the two ends of the umbilical cord are quickly clamped with sterile hemostatic forceps, and a 15-25 cm segment of the umbilical cord is cut from the middle and stored in a sterile container containing a specific sterile preservation solution. Existing storage containers include, for example, a storage box for umbilical cord tissue disclosed in CN222967786U. This storage box includes a cylindrical main body with sidewalls and a bottom, and an opening at the top. The main body is divided into an upper chamber and a lower chamber. The lower chamber is separated from the main body by a ruptureable membrane, which is connected to the inside of the main body to ensure the closure of the lower chamber. The device is designed with only one storage box. Medical staff usually hold the storage box and put the umbilical cord sample into it. Medical staff need to carefully put the end of the umbilical cord into the storage box. If the operation is not careful, the umbilical cord sample may come into contact with the outer wall of the storage box, increasing the probability of umbilical cord contamination. Furthermore, when cleaning the umbilical cord tissue sample in the storage box, it is necessary to transfer the umbilical cord tissue sample to other locations, which also increases the probability of umbilical cord contamination.

[0003] While existing technologies may already offer solutions to the aforementioned problems, this case aims to provide an alternative or replacement technical solution. Utility Model Content

[0004] To address the problems mentioned in the background art, this utility model is implemented through the following technical solution: a stem cell sampling device, comprising an outer tube for sample retention, an inner tube structure provided on the outer tube for sample retention, and a cap structure movably fitted onto the upper end of the outer tube for sample retention;

[0005] The inner tube structure includes a tube body, which comprises a bottom tube, a middle tube, and a head tube. The bottom tube is movably inserted into the lower wall of the outer sample retention tube. The middle tube is located at one end of the bottom tube inside the outer sample retention tube. The head tube is located at the upper end of the middle tube, and its outer wall is movably attached to the inner wall of the outer sample retention tube. A sealing component is installed at the outer end of the bottom tube outside the outer sample retention tube. A mesh is fixedly installed on the inner wall of the bottom tube inside the outer sample retention tube. A rubber diaphragm is fixedly installed at the center of the inner wall of the middle tube. Multiple limiting rods are installed in a circular array at the center of the upper wall of the rubber diaphragm. The bottom tube, sealing component, middle tube, and rubber diaphragm form a liquid storage space filled with a treatment liquid. A guide component is provided between the outer wall of the middle tube and the outer sample retention tube.

[0006] Preferably, the sealing structure includes a plug, which is movably inserted into the lower end of the bottom tube, and a bottom cover is installed at the lower end of the plug, which is movably fitted onto the bottom tube by means of threads.

[0007] Preferably, the cap structure includes a top cover, both ends of which are provided with grooves that match the sample retention tube, and a cone rod is installed in the groove at one end of the top cover.

[0008] Preferably, the guiding assembly includes a guide rod, which is installed on the inner bottom surface of the sample retention tube. A connecting block is movably fitted on the guide rod, and the connecting block is fixedly installed on the outer wall surface of the middle section tube. A spring is installed between the connecting block and the inner bottom surface of the sample retention tube.

[0009] Preferably, the lower end of the sample retention tube is fitted with a tail cap via a threaded connection, and the bottom cap is movably fitted to the inner bottom surface of the tail cap.

[0010] Preferably, the groove is connected to the sample retention tube by a thread.

[0011] Preferably, the outer wall surface of the sample retention tube is provided with multiple raised strips.

[0012] Preferably, one end of the plug has a conical surface.

[0013] Beneficial effects

[0014] This invention provides a stem cell sampling device, which has the following advantages compared with the prior art: The device adopts a double-layer structure of a sample retention tube and a tube body. When placing umbilical cord tissue samples, under the action of the guiding component, the head tube extends out of the top of the sample retention tube, minimizing contact between the umbilical cord tissue sample and the outer wall of the sample retention tube, thus improving the sterility of the umbilical cord tissue sample. When processing the umbilical cord tissue sample, the tail cap and top cap are removed, and PBS is discharged from the bottom tube. The mesh is used to prevent the umbilical cord tissue sample from being discharged from the bottom tube. Then, the limiting rod and the ruptured rubber diaphragm are removed from the tube body, while the umbilical cord tissue sample remains in the tube body. The processing liquid is poured into the tube body to rinse the umbilical cord tissue sample, eliminating the need to transfer the umbilical cord tissue sample to other parts of the body and reducing the risk of contamination of the umbilical cord tissue sample. Attached Figure Description

[0015] Figure 1 This is a three-dimensional exploded structural diagram of a stem cell sampling device according to the present invention.

[0016] Figure 2 This is a partial three-dimensional structural diagram of a stem cell sampling device according to the present invention.

[0017] Figure 3 This is a schematic diagram of the front cross-sectional structure of a stem cell sampling device according to the present invention.

[0018] In the diagram: 1. Sample retention tube, 2. Tube body, 201. Bottom tube, 202. Middle section tube, 203. Head tube, 3. Separator, 4. Rubber diaphragm, 5. Limiting rod, 6. Treatment fluid, 7. Plug column, 8. Bottom cover, 9. Top cover, 10. Conical rod, 11. Guide rod, 12. Connecting block, 13. Spring, 14. Tail cover, 15. Protruding strip. Detailed Implementation

[0019] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0020] Example: Please refer to Figure 1-3 A stem cell sampling device includes a sample retention outer tube 1, an inner tube structure provided on the sample retention outer tube 1, and a cap structure movably fitted on the upper end of the sample retention outer tube 1.

[0021] It should be noted that when not in use, the outer sample tube 1, the inner tube structure, and the cap structure can be packaged in a sealed medical plastic bag to ensure the sterility of the device. When the device is needed, it can be taken out of the medical plastic bag. The outer sample tube 1, the inner tube structure, and the cap structure are used to store umbilical cord tissue samples.

[0022] Specifically, the inner tube structure includes a tube body 2, which includes a bottom tube 201, a middle section tube 202, and a head tube 203. The bottom tube 201 is movably inserted into the lower wall of the sample retention outer tube 1. The middle section tube 202 is located at one end of the bottom tube 201 inside the sample retention outer tube 1. The head tube 203 is located at the upper end of the middle section tube 202, and its outer wall is movably attached to the inner wall of the sample retention outer tube 1. A plug is installed at the outer end of the bottom tube 201 outside the sample retention outer tube 1. The components include a bottom tube 201 with a mesh 3 fixedly installed on the inner wall of one end inside the sample retention tube 1, a rubber diaphragm 4 fixedly installed at the center of the inner wall of the middle tube 202, and multiple limiting rods 5 installed in a circular array at the center of the upper wall of the rubber diaphragm 4. The bottom tube 201, the sealing component, the middle tube 202 and the rubber diaphragm 4 form a liquid storage space, which is filled with a treatment liquid 6. A guide component is provided between the outer wall of the middle tube 202 and the sample retention tube 1.

[0023] It should be noted that the tube body 2 consists of a bottom tube 201, a middle tube 202 and a head tube 203. The treatment solution 6 is PBS, i.e., phosphate buffered salt solution. The sealing structure is sealed at the lower end of the bottom tube 201, and the rubber diaphragm 4 is sealed in the center of the middle tube 202 to seal the PBS in the tube body 2 and prevent the PBS from spilling out of the device when the device is not in use.

[0024] Specifically, the cap structure includes a top cover 9, both ends of which are provided with grooves that match the sample retention tube 1, and a cone rod 10 is installed in the groove at one end of the top cover 9.

[0025] Specifically, the guide assembly includes a guide rod 11, which is installed on the inner bottom surface of the sample retention tube 1. A connecting block 12 is movably mounted on the guide rod 11. The connecting block 12 is fixedly installed on the outer wall of the middle section tube 202. A spring 13 is installed between the connecting block 12 and the inner bottom surface of the sample retention tube 1.

[0026] Specifically, the sealing structure includes a plug 7, which is movably inserted into the lower end of the bottom tube 201. A bottom cover 8 is installed at the lower end of the plug 7, and the bottom cover 8 is movably fitted onto the bottom tube 201 by a thread.

[0027] It should be noted that when the sealing structure is installed in the bottom tube 201, the plug 7 extends into the bottom tube 201, which is equivalent to reducing the gap in the bottom tube 201 to save the amount of PBS used. The bottom cover 8 is used to seal the end of the bottom tube 201 to prevent PBS leakage.

[0028] As a preferred and further option, the lower end of the sample retention tube 1 is fitted with a tail cap 14 via a threaded movable sleeve, and the bottom cap 8 is movably fitted to the inner bottom surface of the tail cap 14.

[0029] Preferably, the groove is further connected to the sample retention tube 1 by a thread;

[0030] It should be noted that, before the device stores umbilical cord tissue samples, the tail cap 14 is threaded onto the lower end of the sample retention tube 1, while the groove on the top cap 9, without the cone rod 10, matches the sample retention tube 1. At this time, the spring 13 is compressed. After the umbilical cord tissue sample is taken, the top cap 9 is removed. Under the restoring force of the spring 13, the connecting block 12 moves on the guide rod 11, and the tube body 2 moves towards the top of the sample retention tube 1. At this time, the head tube 203 extends out from the top of the sample retention tube 1, and medical personnel insert the umbilical cord tissue sample from... The head tube 203 is inserted between multiple limiting rods 5 on the rubber diaphragm 4 inside the middle tube 202. The purpose of the head tube 203 extending beyond the top of the sample retention tube 1 is to minimize contact between the umbilical cord tissue sample and the outer wall of the sample retention tube 1, thereby improving the sterility of the umbilical cord tissue sample. Then, the end of the conical rod 10 is inserted into the head tube 203 and rotated to connect with the sample retention tube 1 through the groove on the top cover 9. This causes the conical rod 10 to puncture and cut the rubber diaphragm 4, allowing the PBS to come into contact with the umbilical cord tissue sample. The device is then transferred to the stem cell sample processing department.

[0031] When processing umbilical cord tissue samples, the tail cap 14 and top cap 9 are removed, and PBS is discharged from the bottom tube 201. The mesh 3 is used to prevent the umbilical cord tissue sample from being discharged from the bottom tube 201. Then, multiple limiting rods 5 and the ruptured rubber diaphragm 4 are removed from the tube body 2, while the umbilical cord tissue sample remains in the tube body 2. This operation is carried out under sterile conditions. Then, sterile PBS or physiological saline containing antibiotics and antifungal agents is poured into the tube body 2 to repeatedly rinse the umbilical cord tissue sample to remove residual blood and contaminants. There is no need to transfer the umbilical cord tissue sample to other parts of the body, reducing the risk of contamination of the umbilical cord tissue sample.

[0032] As a preferred and further option, a plurality of protrusions 15 are provided on the outer wall of the sample retention tube 1. When the operator's hand grips the outer wall of the sample retention tube 1, the protrusions 15 are used to increase the friction between the operator's hand and the outer wall of the sample retention tube 1.

[0033] As a preferred and further option, one end of the plug 7 is provided with a conical surface to facilitate insertion into the bottom tube 201;

[0034] Except for spring 13, all other components in the device are made of medical-grade plastic materials, resulting in a simple structure and low production cost.

[0035] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sampling device for stem cells comprising a sample retaining outer tube (1), characterised in that, The outer tube (1) for sample retention is provided with an inner tube structure, and the upper end of the outer tube (1) for sample retention is movably fitted with a buckle structure; The inner tube structure includes a tube body (2), which includes a bottom tube (201), a middle section tube (202), and a head tube (203). The bottom tube (201) is movably inserted into the lower wall of the sample retention outer tube (1). The middle section tube (202) is located at one end of the bottom tube (201) inside the sample retention outer tube (1). The head tube (203) is located at the upper end of the middle section tube (202), and the outer wall of the head tube (203) is movably attached to the inner wall of the sample retention outer tube (1). The bottom tube (201) is equipped with a... The sealing assembly has a mesh (3) fixedly installed on the inner wall of one end of the bottom tube (201) inside the sample retention tube (1). A rubber diaphragm (4) is fixedly installed at the center of the inner wall of the middle section tube (202). Multiple limiting rods (5) are installed in a circular array at the center of the upper wall of the rubber diaphragm (4). The bottom tube (201), the sealing assembly, the middle section tube (202) and the rubber diaphragm (4) form a liquid storage space, which is filled with treatment liquid (6). A guide assembly is provided between the outer wall of the middle section tube (202) and the sample retention tube (1).

2. The stem cell sampling device according to claim 1, characterized in that, The sealing assembly includes a plug (7), which is movably inserted into the lower end of the bottom tube (201). A bottom cover (8) is installed at the lower end of the plug (7), and the bottom cover (8) is movably fitted onto the bottom tube (201) by means of threads.

3. The stem cell sampling device according to claim 1, characterized in that, The cap structure includes a top cover (9), both ends of which are provided with grooves that match the sample retention tube (1), and a cone rod (10) is installed in the groove at one end of the top cover (9).

4. A stem cell sampling device according to claim 1, characterized in that, The guiding assembly includes a guide rod (11), which is installed on the inner bottom surface of the sample retention tube (1). A connecting block (12) is movably fitted on the guide rod (11). The connecting block (12) is fixedly installed on the outer wall surface of the middle section tube (202). A spring (13) is installed between the connecting block (12) and the inner bottom surface of the sample retention tube (1).

5. A stem cell sampling device according to claim 2, characterized in that, The lower end of the sample retention tube (1) is fitted with a tail cap (14) via a threaded sleeve, and the bottom cap (8) is movably fitted to the inner bottom surface of the tail cap (14).

6. A stem cell sampling device according to claim 3, characterized in that, The groove is connected to the sample retention tube (1) by a thread.

7. A stem cell sampling device according to claim 1, characterized in that, The outer wall of the sample retention tube (1) is provided with multiple protrusions (15).

8. A stem cell sampling device according to claim 2, characterized in that, One end of the plug (7) has a conical surface.

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