A fully automatic multifunctional workstation

Abstract

This utility model discloses a fully automated multifunctional workstation in the field of biological experimental equipment. It includes an automated culture system and a safety cabinet that are isolated from each other, with a transfer component between them to transport the target carrier. The safety cabinet contains multiple robotic arms at the top and various components at the bottom, including a live cell detection component, a shaker, and a liquid addition component. A pipetting system drives the movement of some components. It also includes a liquid addition component with a heating element, a specially structured cap opening and closing component, and a liquid aspiration component with disposable pipette tips. Furthermore, the safety cabinet contains a waste disposal component and a sterilization component, and on one side are a consumable storage component and a cryopreservation component. This application achieves automated operation of biological experiments, enabling multiple functions such as culture, observation, liquid addition, cap opening and closing, and centrifugation of the target carrier. It also possesses functions such as waste disposal, sterilization, consumable storage, and sample cryopreservation, thereby improving experimental efficiency and safety.

Description

Technical Field

[0001] This utility model relates to the technical field of biological experimental equipment, and more specifically, to a fully automatic multifunctional workstation. Background Technology

[0002] In today's biological research field, with the continuous growth in the number of domestic biological experiments, the demand for cell culture, virus experiments, and bacterial experiments is increasing. These experiments are of great significance in the medical field, such as assisting in disease diagnosis, optimizing treatment plans, promoting the development of new drugs, and fostering the development of the biotechnology industry. However, existing related products are often single-function and fail to meet the diversified needs of the market. This imposes numerous limitations on researchers when conducting various biological experiments, hindering the efficient completion of a series of tasks from basic research to clinical applications, and seriously affecting the efficiency and progress of scientific research. This not only hinders innovative breakthroughs in biomedicine but also impedes the enhancement of my country's competitiveness in the global biological research field. Therefore, the development of multifunctional and highly efficient experimental equipment has become an urgent need for the industry's development.

[0003] Previously, cell culture, virus experiments, and bacterial experiments mostly relied on artificial culture methods. Researchers had to manually perform cell passage procedures, including a series of complex steps such as washing, digestion, centrifugation, and dilution, and then transfer the cells to new culture media. Observing cell growth also required manual viability testing. Other tasks, such as opening and closing the culture media caps, adding culture medium, and removing waste liquid, also relied on manual operation. Furthermore, artificial culture involved sample storage and transportation, each step requiring manual intervention from the researchers. While this traditional method of artificial culture could accomplish experimental tasks to a certain extent, it has proven inadequate for the demands of modern large-scale, high-precision biological experiments.

[0004] Artificial culture methods have significant drawbacks. The highly repetitive nature of the experimental process leads to extreme workload, easily causing fatigue among researchers and increasing the probability of errors. Furthermore, the sheer volume of work involved in artificial culture consumes a substantial amount of time and energy. In addition, differences in operating habits and standards among researchers make it difficult to standardize culture procedures, affecting the accuracy and comparability of experimental results. Simultaneously, manual operation requires a large amount of consumables, significantly increasing resource consumption and experimental costs. Moreover, artificial culture is relatively slow, making it unsuitable for large-scale experiments. Finally, the direct contact between researchers and the target material during the experiment poses certain risks and may threaten their health.

[0005] In conclusion, designing a fully automated, multifunctional workstation that can replace manual labor, has automatic culture, subculture, and experimental functions, and is compatible with multiple carriers is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0006] In view of this, the purpose of this utility model is to provide a fully automatic multifunctional workstation that effectively solves the problems of high repetition, low efficiency, and danger of manual operation in the field of biological experiments. Through the integration of an automated system and modular design, it realizes full-process automation of cell culture, virus experiments, and fungal experiments.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A fully automated multifunctional workstation includes an automated culture system and a safety cabinet that are isolated from each other. A transfer component is provided between the automated culture system and the safety cabinet to transport the target carrier in the automated culture system to the safety cabinet.

[0009] The safety cabinet has an installation space inside. Multiple robotic arms are installed at the top of the safety cabinet in the installation space. At the bottom of the safety cabinet in the installation space, there are a live cell detection component for observing the state of the target carrier, a shaker for adjusting the angle of the target carrier, a liquid addition component for adding liquid to the target carrier, a cover opening and closing component for opening and closing the cover of the target carrier, a liquid suction component for aspirating waste liquid or separating liquid from the target carrier, a centrifugation component for centrifuging and extracting the target carrier, and an incubation component for heating and shaking the target carrier.

[0010] The safety cabinet is equipped with a pipetting system. The shaker, the liquid addition assembly, the liquid aspiration assembly, and the switch cap assembly are integrated into the pipetting system. The pipetting system is used to drive the shaker, the liquid addition assembly, the liquid aspiration assembly, and the switch cap assembly to move horizontally or vertically, respectively.

[0011] Preferably, the liquid addition assembly includes a liquid addition pipe and a heating element. The liquid addition pipe is connected to a container for storing culture medium, and the heating element is used to heat the culture medium in the liquid addition pipe to a specified temperature.

[0012] Preferably, the pipetting system includes an electric slide rail and a plurality of lifting components disposed on the electric slide rail, wherein the plurality of lifting components are respectively connected to the shaker, the liquid addition component, the liquid aspiration component and the cover opening and closing component.

[0013] Preferably, the cover assembly includes a clamping member and a driving member, wherein the clamping member is used to clamp the cover of the target carrier, and the driving member is used to drive the clamping member to perform the opening and closing operation of the cover.

[0014] Preferably, the aspiration assembly includes an aspiration gun disposed on one side of the pipetting system, and the aspiration gun is equipped with a detachable disposable pipette tip.

[0015] Preferably, the centrifugation assembly includes a centrifuge disposed within the safety cabinet.

[0016] Preferably, the safety cabinet is further provided with a waste disposal component within the installation space. The waste disposal component includes an installation slot opened in the safety cabinet, a waste collection box disposed in the installation slot, and an automatic door disposed at the opening of the waste collection box.

[0017] Preferably, the safety cabinet is equipped with a disinfection component within the installation space. The disinfection component includes multiple disinfection nozzles disposed within the safety cabinet, which are used to spray disinfectant into the safety cabinet.

[0018] Preferably, the safety cabinet is provided with multiple consumable storage racks, and consumables are placed in the multiple consumable storage racks;

[0019] A consumable storage component is provided on one side of the safety cabinet. The consumable storage component includes a rotary switcher and multiple consumable racks arranged circumferentially along the surface of the rotary switcher.

[0020] Preferably, a cryopreservation assembly is provided on one side of the safety cabinet, the cryopreservation assembly including a cryopreservation shell and cryopreservation tubes installed inside the cryopreservation shell.

[0021] This utility model provides a fully automatic multifunctional workstation, in which the automatic culture system and the safety cabinet are isolated from each other and the target carrier is transported through a transfer component, ensuring the safety of the operating environment; multiple robotic arms are set on the top of the safety cabinet to replace manual labor in tasks such as carrier transfer, improving work efficiency; a live cell detection component can observe the state of the target carrier, which helps to monitor the experimental progress; a shaker can adjust the angle of the target carrier for convenient liquid aspiration and addition; a liquid addition component can add liquid to the target carrier to meet experimental needs; a cap opening and closing component can open and close the cap of the target carrier; a liquid aspiration component can aspirate waste liquid from the target carrier or perform liquid separation; a centrifugation component can centrifuge and extract the target carrier; an incubation component can heat and shake the target carrier to accelerate digestion, mixing, and recovery processes; the pipetting system integrates the shaker, liquid addition component, liquid aspiration component, and cap opening and closing component, and can drive them to move horizontally or vertically, realizing the rapid installation and flexible operation of the multifunctional module, improving the automation level and compatibility of the workstation, and solving the problems of large workload and limited functions in artificial culture. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0023] Figure 1 This is a schematic diagram of the overall structure of the fully automatic multi-functional workstation in this embodiment;

[0024] Figure 2 This is a top view of the fully automated multifunctional workstation in this embodiment.

[0025] Figures 1-2 In the accompanying drawings, the reference numerals include:

[0026] 1. Automated culture system; 2. Safety cabinet; 3. Robotic arm; 4. Live cell detection component; 5. Shaker; 6. Liquid addition component; 7. Liquid aspiration component; 8. Waste disposal component; 9. Lid opening and closing component; 10. Centrifugation component; 11. Consumables pre-positioning rack; 12. Pipette system; 13. Sterilization component; 14. Consumables storage component; 15. Incubation component; 16. Cryopreservation component. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this utility model pertains. The terms "first," "second," and similar words used in this utility model do not indicate any order, quantity, or importance. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. Terms such as "up," "down," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly. This application discloses a fully automatic multifunctional workstation.

[0029] The core of this utility model is to provide a fully automatic multifunctional workstation.

[0030] Please refer to Figures 1 to 2 .

[0031] The fully automatic multifunctional workstation provided by this utility model includes an automatic culture system 1 and a safety cabinet 2, which are isolated from each other. A transfer component is provided between the automatic culture system 1 and the safety cabinet 2 to transport the target carrier in the automatic culture system 1 to the safety cabinet 2. The safety cabinet 2 has an installation space. Multiple robotic arms 3 are set at the top of the safety cabinet 2 in the installation space. At the bottom of the safety cabinet 2 in the installation space, there are a live cell detection component 4 for observing the state of the target carrier, a shaker 5 for adjusting the angle of the target carrier, a liquid addition component 6 for adding liquid to the target carrier, a cover opening and closing component 9 for opening and closing the cover of the target carrier, a suction component 7 for aspirating waste liquid or separating liquid from the target carrier, a centrifugation component 10 for centrifuging and extracting the target carrier, and an incubation component 15 for heating and shaking the target carrier. The safety cabinet 2 is equipped with a pipetting system 12. The shaker 5, the liquid addition component 6, the suction component 7, and the cover opening and closing component 9 are integrated into the pipetting system 12. The pipetting system 12 is used to drive the shaker 5, the liquid addition component 6, the suction component 7, and the cover opening and closing component 9 to move horizontally or vertically, respectively.

[0032] Specifically, the automated culture system 1 and the biosafety cabinet 2 are isolated from each other, and the target carrier in the automated culture system 1 is transferred to the biosafety cabinet 2 via a transfer component. Multiple robotic arms 3 are installed at the top of the biosafety cabinet 2, and a live cell detection component 4, a shaker 5, a liquid addition component 6, a cap opening / closing component 9, a liquid aspiration component 7, a centrifugation component 10, and an incubation component 15 are installed at the bottom. The shaker 5, liquid addition component 6, liquid aspiration component 7, and cap opening / closing component 9 are integrated into a pipetting system 12, which can drive them to move horizontally or vertically. This layout allows for a clear division of labor among the workstation components. The robotic arm 3 can simulate a human hand to perform tasks such as carrier transfer. The live cell detection component 4 can monitor the status of the target carrier in real time. The shaker 5 can adjust the carrier angle for easy aspiration and addition of liquid. The liquid addition component 6 can add liquid to the carrier. The cap opening and closing component 9 can perform the opening and closing operation of the carrier. The aspiration component 7 can aspirate waste liquid or separate liquid. The centrifugation component 10 is used for centrifugation to extract the target substance. The incubation component 15 can heat and shake the target carrier. Furthermore, the pipetting system 12 allows for flexible adjustment of the positions of each component. This enables the workstation to automatically complete multiple operations such as cell culture, virus experiments, and bacterial experiments, improving work efficiency, reducing manual labor intensity, and avoiding non-standard problems that may be caused by manual operation.

[0033] It should be noted that the automated culture system 1 is a relatively independent area used for the initial culture of the target vector. It can employ a suitable chamber structure, internally equipped with temperature and humidity control devices, lighting devices, etc., to meet the culture conditions of different cells, viruses, microorganisms, etc. For example, the chamber can be made of high-strength plastic or metal with good thermal insulation properties. The temperature and humidity control device can include sensors and regulating equipment, such as humidifiers, dehumidifiers, and thermostats, capable of precisely controlling the temperature and humidity within the chamber. The lighting device can provide appropriate light intensity and spectrum according to different culture requirements. The transfer component can be a conveyor belt or a robotic arm. If a conveyor belt is used, it can be driven by a motor, transporting the target vector from the automated culture system to the safety cabinet via a belt. The surface of the conveyor belt can be made of anti-slip material to ensure that the target vector does not slip during transportation. If a robotic arm is used, it can accurately grasp and place the target vector through multi-joint movements, and the grasping force can be adjusted as needed.

[0034] It should also be noted that safety cabinet 2 is the core working area of ​​the entire workstation, providing a safe environment for the internal components. Safety cabinet 2 uses front and rear dual electric lifting doors or a single side lifting door. The outer shell of safety cabinet 2 can be made of stainless steel, which has good corrosion resistance and sealing performance. The internal installation space is spacious, allowing for the rational arrangement of various components. Multiple robotic arms 3 are suspended from the top of the safety cabinet. Robotic arms 3 can be multi-jointed industrial robotic arms, and their ends can be equipped with different grippers to adapt to different types of target carriers. For example, a claw-type gripper can be used for culture flasks, and a suction cup-type gripper can be used for orifice plates. The robotic arms 3 are driven by motors and a control system, which can precisely control their movement trajectory and force to complete tasks such as carrier transfer, opening and closing lids, and replacing consumables.

[0035] In some other embodiments, the robotic arm 3 may also be a magnetically levitated robotic arm. The magnetically levitated robotic arm achieves movement through magnetic force, eliminating the need for traditional mechanical transmission components, reducing friction and wear, and improving the movement accuracy and reliability of the robotic arm 3. The joints of the magnetically levitated robotic arm utilize a combination of electromagnetic coils and permanent magnets; by controlling the magnitude and direction of the current in the electromagnetic coils, multi-degree-of-freedom movement of the robotic arm can be achieved.

[0036] Optionally, a pneumatic transfer device is used for the transfer assembly. This device includes pipes and pneumatic valves. The pipes can be made of plastic or metal and offer good airtightness. The pneumatic valves are controlled by air pressure, enabling rapid and accurate transfer of the target carrier. When the target carrier needs to be transferred from the automated culture system to the biosafety cabinet, the air pressure is adjusted to open the corresponding pneumatic valve, and the target carrier moves rapidly along the pipe into the biosafety cabinet under the influence of airflow. This method offers advantages such as high transfer speed and high accuracy, further improving the workstation's efficiency.

[0037] The aforementioned fully automated multifunctional workstation features an automated culture system 1 isolated from the safety cabinet 2, with the target carrier transported via a transfer assembly, ensuring a safe operating environment. Multiple robotic arms 3 on the top of the safety cabinet 2 can replace manual labor in tasks such as carrier transfer, improving work efficiency. A live cell detection assembly 4 can observe the target carrier's state, helping to monitor the experimental progress. A shaker 5 can adjust the target carrier's angle for convenient liquid aspiration and addition. A liquid addition assembly 6 can add liquid to the target carrier to meet experimental needs. A cap opening / closing assembly 9 can open and close the target carrier's cap. A liquid aspiration assembly 7 can aspirate waste liquid from the target carrier or perform liquid separation. A centrifugation assembly 10 can centrifuge and extract the target carrier. An incubation assembly 15 can heat and shake the target carrier to accelerate digestion, mixing, and recovery processes. A pipetting system 12 integrates the shaker 5, liquid addition assembly 6, liquid aspiration assembly 7, and cap opening / closing assembly 9, and can drive them horizontally or vertically, enabling rapid installation and flexible operation of the multifunctional module. This improves the workstation's automation and compatibility, solving problems such as high workload and limited functionality inherent in manual culture.

[0038] The fully automatic multifunctional workstation provided by this utility model will be described in more detail below with reference to the accompanying drawings and specific embodiments.

[0039] In one specific embodiment, the live cell detection component 4 is used to observe the growth status of cells in the target carrier, and it can be a device such as a microscope. The microscope can be an electron microscope or an optical microscope, with high resolution and magnification, enabling clear observation of the cell morphology and structure. The microscope can be mounted on an adjustable stand to adjust the observation angle and position.

[0040] In one specific embodiment, the shaker 5 is used to adjust the angle of the target carrier, facilitating liquid aspiration and addition operations. The shaker 5 can be an electric shaker, driven by a motor, capable of achieving different shaking modes and speeds. For example, it can perform circular shaking, reciprocating shaking, etc. The platform of the shaker can be made of rubber or silicone to increase friction and prevent the target carrier from slipping.

[0041] In one specific implementation, reference is made to... Figure 2The liquid addition assembly 6 includes a liquid addition pipe and a heating element. The liquid addition pipe is connected to a container for storing culture medium, and the heating element is used to heat the culture medium in the liquid addition pipe to a specified temperature.

[0042] Specifically, the liquid addition assembly 6 has a liquid addition pipe connected to a container storing culture medium, and a heating element that works in conjunction with the liquid addition pipe. When the culture medium enters the liquid addition pipe from the container, the heating element heats the culture medium in the liquid addition pipe to a specified temperature. This structure allows the liquid addition assembly to provide culture medium at a suitable temperature to the target carrier, avoiding the impact of unsuitable culture medium temperature on the growth environment of cells, viruses, or bacteria in the target carrier, thereby improving the success rate of culture and experiments.

[0043] The liquid addition assembly 6 is used to add the culture medium, which is stored at low temperature in the container, to the carrier after being instantaneously heated to a suitable temperature. The liquid addition pipeline can be made of stainless steel, which has good corrosion resistance and hygiene, and can be reused without consumables. The heating element can be an electric heating wire or heating plate, which is wrapped around the outside of the liquid addition pipeline. The heating temperature of the culture medium is precisely controlled by a temperature sensor and a control system.

[0044] Optionally, the heating element employs microwave heating. Microwave heating can rapidly and evenly heat the culture medium, improving heating efficiency. The microwaves generated by the microwave generator act directly on the culture medium in the liquid addition pipe, causing the culture medium molecules to vibrate rapidly and generate heat. Simultaneously, a temperature sensor and control system precisely control the heating time and power to prevent overheating of the culture medium.

[0045] Based on any of the above embodiments, refer to Figure 2 The pipetting system 12 includes an electric slide rail and multiple lifting components mounted on the electric slide rail. The multiple lifting components are respectively connected to the shaker 5, the liquid addition component 6, the liquid aspiration component 7, and the cover opening and closing component 9.

[0046] Specifically, the motorized slide rail of the pipetting system 12 is located inside the safety cabinet 2. Multiple lifting components are mounted on the motorized slide rail and are respectively connected to the shaker 5, the liquid addition component 6, the liquid aspiration component 7, and the cap opening / closing component 9. The motorized slide rail drives the lifting components to move horizontally, while the lifting components themselves drive the connected shaker 5, liquid addition component 6, liquid aspiration component 7, and cap opening / closing component 9 to move vertically. This allows the shaker 5, liquid addition component 6, liquid aspiration component 7, and cap opening / closing component 9 to move flexibly in both horizontal and vertical directions, thereby accurately and efficiently completing operations such as liquid addition, liquid aspiration, and cap opening / closing on the target carrier, effectively improving the workstation's work efficiency and operational accuracy. It also allows each component to flexibly reach the required operating position on the target carrier, enhancing operational flexibility and accuracy, and further improving the workstation's automation level and work efficiency.

[0047] It should be noted that the electric slide rail can be a linear guide rail, with a motor driving a slider to slide on the rail. The lifting assembly can be a screw jack or a cylinder, achieving height adjustment of each component through lifting motion. The pipetting system 12 allows each component to move flexibly in both horizontal and vertical directions, improving the workstation's efficiency and flexibility.

[0048] Based on any of the above embodiments, refer to Figure 2 The cover assembly 9 includes a clamping component and a driving component. The clamping component is used to clamp the cover of the target carrier, and the driving component is used to drive the clamping component to perform the opening and closing operation of the cover.

[0049] Specifically, the cover opening / closing assembly 9 is located within the installation space of the safety cabinet and integrated into the pipetting system 12, which can drive it to move horizontally or vertically. The cover opening / closing assembly 9 includes a clamping member for holding the cover of the target carrier and a driving member for opening and closing the cover. The driving member drives the clamping member to move, which can realize the automatic opening and closing of the cover of the target carrier, avoid manual operation, reduce workload, improve work efficiency, and ensure standardized operation.

[0050] It should be noted that the clamping component can be a gripper made of elastic material to avoid damaging the cover. The driving component can be a motor or a cylinder, which drives the clamping component to move through a transmission mechanism.

[0051] Based on any of the above embodiments, refer to Figure 2 The aspiration assembly 7 includes an aspiration gun disposed on one side of the pipetting system 12, and the aspiration gun is equipped with a detachable disposable pipette tip.

[0052] Specifically, the pipette is located on one side of the pipetting system 12 and is equipped with a detachable disposable pipette tip. When it is necessary to aspirate waste liquid from the target carrier or perform liquid separation, the operation can be completed using the pipette and the disposable pipette tip. After use, the disposable pipette tip can be removed and replaced to avoid cross-contamination and ensure the safety and accuracy of the aspiration operation. The pipette can be an electric pipette, which uses the principle of negative pressure to aspirate waste liquid from the target carrier or perform liquid separation. The disposable pipette tip can be replaced as needed to ensure hygiene and experimental accuracy.

[0053] Based on any of the above embodiments, refer to Figure 2 The centrifugation assembly 10 includes a centrifuge housed within the safety cabinet 2.

[0054] Specifically, the centrifuge, housed within a biosafety cabinet, is used for the centrifugal extraction of target carriers. Once the target carrier is placed in the centrifuge, its high-speed operation effectively separates the target substance from the carrier, facilitating subsequent research or experimental operations. This avoids the tediousness and uncertainty of manual operation, improving work efficiency and accuracy. The centrifuge can be a high-speed centrifuge, providing sufficient centrifugal force to separate substances from the target carrier. The centrifuge rotor can be selected from different specifications to accommodate different types of target carriers.

[0055] Based on any of the above embodiments, refer to Figure 2 The safety cabinet 2 is located within the installation space and is also equipped with a waste disposal component 8. The waste disposal component 8 includes an installation slot opened in the safety cabinet 2, a waste collection box set in the installation slot, and an automatic door set at the opening of the waste collection box.

[0056] Specifically, the waste disposal component 8 includes an installation slot within a safety cabinet, a waste collection bin located within the slot, and an automatic door at the bin's opening. When waste needs to be disposed of, the automatic door opens automatically, allowing the waste to be disposed of into the waste collection bin. This effectively facilitates centralized collection and disposal of waste, preventing indiscriminate waste disposal from polluting the work environment.

[0057] It should be noted that the automatic door can be electrically controlled, automatically opening when the robotic arm approaches to pick up waste, allowing the waste to be smoothly deposited into the waste collection bin; or it can be controlled by sensors, such as infrared sensors, which automatically open the bin when they detect the approach of waste and automatically close it after the waste has been deposited, thereby effectively preventing waste from being exposed inside the biosafety cabinet and causing contamination, keeping the environment inside the biosafety cabinet clean, and ensuring the safety and accuracy of the experiment.

[0058] Based on any of the above embodiments, refer to Figure 1 The safety cabinet 2 is located in the installation space and is equipped with a disinfection component 13. The disinfection component 13 includes multiple disinfection nozzles installed inside the safety cabinet 2. The multiple disinfection nozzles are used to spray disinfection inside the safety cabinet 2.

[0059] Specifically, a disinfection assembly 13 is installed within the installation space of the biosafety cabinet. This assembly consists of multiple disinfection nozzles located inside the cabinet. These nozzles can directly spray disinfectant into the interior of the biosafety cabinet, primarily targeting the waste collection bin, pipetting system 12, and other spray areas within the biosafety cabinet. This placement and operation effectively disinfects waste materials, waste liquids, and consumables within the biosafety cabinet, preventing contamination and ensuring the safety and cleanliness of the experimental environment. The disinfection nozzles can be atomizing nozzles to ensure the disinfectant is evenly sprayed within the biosafety cabinet.

[0060] Based on any of the above embodiments, refer to Figure 1 The safety cabinet 2 is equipped with multiple consumable pre-placement racks 11, and consumables are placed in the multiple consumable pre-placement racks 11; a consumable storage component 14 is provided on one side of the safety cabinet 2, and the consumable storage component 14 includes a rotary switcher and multiple consumable racks arranged circumferentially along the surface of the rotary switcher.

[0061] Specifically, the consumable pre-placement rack 11 is used to place consumables (such as disposable pipette tips for the aforementioned suction gun), target objects, etc. The consumable storage component 14 is used to store various consumables required for the experiment, providing consumables for the entire workstation. Multiple consumable pre-placement racks 11 are provided inside the biosafety cabinet 2. The consumable pre-placement racks 11 can be located at the bottom or on the side wall of the biosafety cabinet 2. A consumable storage component 14 is located on one side of the biosafety cabinet. This component includes a rotary switcher and multiple consumable racks arranged circumferentially along its surface. With this layout, the consumable storage component 14 can use the rotary switcher to switch between different consumable racks to replenish the workstation with the required consumables. The consumable pre-placement racks 11 can provide readily available consumables for operations within the station, ensuring that the workstation can promptly obtain various consumables during operation, guaranteeing the smooth and efficient conduct of experiments.

[0062] Based on any of the above embodiments, refer to Figure 2 A cryopreservation assembly 16 is provided on one side of the safety cabinet 2. The cryopreservation assembly 16 includes a cryopreservation shell and cryopreservation tubes installed inside the cryopreservation shell.

[0063] Specifically, the cryopreservation unit is located on one side of the safety cabinet. The cryopreservation unit includes a cryopreservation shell and cryopreservation tubes installed inside the cryopreservation shell. It can be used for the cryopreservation of target objects, enabling the workstation to meet the needs of ultra-low temperature cryopreservation of target objects and improving the versatility and comprehensiveness of the workstation's functions.

[0064] Based on any of the above embodiments, the incubation component 15 is used to heat and agitate the target carrier to accelerate the digestion, mixing, and recovery of the target carrier. The incubation component 15 can be a box with heating and agitation functions, and is equipped with a temperature sensor and an agitation device inside, which can precisely control the temperature and agitation frequency.

[0065] The implementation principle of a fully automated multifunctional workstation according to this application embodiment is as follows: by isolating the automated culture system 1 and the safety cabinet 2, and using a transfer component to transport the target carrier, the independence and safety of the culture environment are ensured. Multiple components within the safety cabinet 2 work collaboratively: the robotic arm 3 handles the transport and manipulation of the carrier; the live cell detection component 4 provides real-time feedback on cell status; the shaker 5, liquid addition component 6, liquid aspiration component 7, cap opening / closing component 9, centrifugation component 10, and incubation component 15 each perform their respective functions; the waste disposal component 8 handles waste; the sterilization component 13 performs sterilization; the consumables pre-positioning rack 11 and consumables storage component 14 provide the necessary consumables; the cryopreservation component 16 cryopreserves the target material; and the pipetting system 12 supports the movement of each component. This clearly defined division of labor and collaborative approach effectively solves the problems of high repetitiveness, large workload, inability to standardize culture methods, large consumption of consumables, slow speed, and high risk in artificial culture methods, greatly improving work efficiency and experimental quality, and bringing convenience and innovation to the field of biological experiments.

[0066] Another core aspect of this utility model is to provide a method for using a fully automatic multi-functional workstation, which is applied to the aforementioned fully automatic multi-functional workstation.

[0067] The method of using the fully automatic multifunctional workstation provided by this utility model includes the following steps;

[0068] S1. The target carrier is placed in the fully automated culture system 1. According to the program settings, the target carrier is transported from the automated culture system 1 to the biosafety cabinet 2 using transfer components (such as conveyor belts, robotic arms, or pneumatic transfer devices). The robotic arm 3 inside the biosafety cabinet 2 picks up the target carrier and sends it to the live cell detection component 4 for observation to understand the growth status of the cells in the target carrier.

[0069] S2, taking cell passage as an example, the robotic arm 3 moves the target vector to the opening and closing cap assembly 9 for opening. Then, the opened vector is moved to the aspiration area. At this time, the pipetting system 12 drives the aspiration assembly 7 to move to the appropriate position. The aspiration gun on the aspiration assembly 7 uses the principle of negative pressure to aspirate the old culture medium or other waste liquid in the target vector through the detachable disposable pipette tip.

[0070] S3, after liquid aspiration, robotic arm 3 moves the carrier to the liquid addition point, and the liquid addition system 12 drives the liquid addition assembly 6 to move into place. The liquid addition pipe of the liquid addition assembly 6 is connected to the container storing the culture medium. The heating element (such as an electric heating wire, heating plate, or microwave heating) heats the culture medium in the liquid addition pipe to the specified temperature, and then adds the culture medium to the target carrier through the liquid addition pipe. After the liquid addition is completed, robotic arm 3 moves the carrier to the cover assembly 9 for the cover operation.

[0071] S4, the robotic arm 3 moves the covered carrier to the shaker 5 or the incubation assembly 15. The shaker 5 can be driven by a motor to adjust the angle of the target carrier, which facilitates subsequent liquid aspiration and addition operations. It can also perform circular shaking, reciprocating shaking, etc., to ensure that the culture medium and the target substance are fully mixed. The incubation assembly 15 can heat and shake the target carrier to accelerate the digestion, mixing, and recovery of the target carrier.

[0072] S5, perform the waste liquid aspiration operation again. The robotic arm 3 moves the carrier to the liquid addition point to add liquid again, and then moves it to the switch cover assembly 9 to open and close the cover, so that the culture medium can be spread evenly and ensure full contact with the surface of the target object.

[0073] S6. For the centrifugation operation involved in cell passage, robotic arm 3 delivers the centrifuge tube to the cap-opening assembly 9 to open the cap. After the shaker 5 is adjusted to the correct angle, robotic arm 3 places the centrifuge tube on the pipetting system 12. The aspiration assembly 7 transfers the cell suspension from the carrier into the centrifuge tube, and then robotic arm 3 delivers it to the cap-opening assembly 9 to tighten the cap. After centrifugation, robotic arm 3 delivers the centrifuge tube to the cap-opening assembly 9 to open the cap and places it on the pipetting system 12. The pipetting system 12 drives the aspiration assembly 7 to remove the supernatant, leaving the cell pellet. Next, robotic arm 3 moves the centrifuge tube to the liquid addition point to add culture medium and shakes to mix.

[0074] S7, robotic arm 3 takes the new carrier to the liquid addition area, adds an appropriate amount of complete culture medium, and places it on shaker 5. The pipetting system 12 drives the aspiration assembly 7 to complete the addition of the cell suspension, and shaker 5 shakes the carrier to mix the cells. Afterwards, robotic arm 3 moves the waste centrifuge tube to the capping assembly 9, screws on the cap, and throws it into the waste collection box of waste disposal assembly 8.

[0075] S8, the robotic arm 3 delivers the new carrier to the microscope at the live cell detection component 4 for observation. If the observation result is satisfactory, the robotic arm 3 places the new carrier on the external tray of the incubator, where it is picked up by the robotic arm 3 inside the incubator and placed on the tray for culture. If the result is unsatisfactory, the carrier is discarded and can be passaged again or used for other experimental operations. Throughout the process, multiple disinfection nozzles of the disinfection component 13 will spray disinfection inside the biosafety cabinet 2 as needed, disinfecting waste materials, waste liquids, consumables, etc. The consumable pre-placement rack 11 and the consumable storage component 14 provide various consumables required for the experiment.

[0076] The aforementioned fully automated multifunctional workstation operates on the basis of the coordinated work of its components, sequentially completing cell culture, passage, and experimental operations according to preset steps. The robotic arm 3 handles the transport and manipulation of the culture medium, while the components move flexibly under the drive of the pipetting system 12, achieving accurate functions such as liquid addition, aspiration, capping, centrifugation, and incubation. Simultaneously, the live cell detection component 4 monitors cell status in real time, the waste disposal component 8 handles waste, the sterilization component 13 ensures a hygienic and safe experimental environment, and the consumables rack 11 and consumables storage component 14 provide ample consumable support. This automated operation effectively avoids the drawbacks of manual culture methods, improves work efficiency and the accuracy and standardization of experiments, reduces experimental risks, and provides a more efficient and reliable solution for biological experiments.

[0077] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0078] The above provides a detailed description of the fully automatic multifunctional workstation provided by this utility model. Specific examples have been used to illustrate the principles and implementation methods of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that those skilled in the art can make several improvements and modifications to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of this utility model.

Claims

1. A fully automatic multifunctional workstation, characterized in that, The system includes an automated culture system (1) and a safety cabinet (2) that are isolated from each other. A transfer component is provided between the automated culture system (1) and the safety cabinet (2) to transport the target carrier in the automated culture system (1) to the safety cabinet (2). The safety cabinet (2) has an installation space inside. The safety cabinet (2) is equipped with multiple robotic arms (3) at the top of the installation space. The safety cabinet (2) is equipped with a live cell detection component (4) for observing the state of the target carrier, a shaker (5) for adjusting the angle of the target carrier, a liquid addition component (6) for adding liquid to the target carrier, a cover opening and closing component (9) for opening and closing the cover of the target carrier, a liquid suction component (7) for absorbing waste liquid or separating liquid from the target carrier, a centrifugation component (10) for centrifuging and extracting the target carrier, and an incubation component (15) for heating and shaking the target carrier. The safety cabinet (2) is equipped with a pipetting system (12). The shaker (5), the liquid addition assembly (6), the liquid aspiration assembly (7), and the switch cover assembly (9) are integrated on the pipetting system (12). The pipetting system (12) is used to drive the shaker (5), the liquid addition assembly (6), the liquid aspiration assembly (7), and the switch cover assembly (9) to move horizontally or vertically, respectively.

2. The fully automatic multifunctional workstation according to claim 1, characterized in that, The liquid addition assembly (6) includes a liquid addition pipe and a heating element. The liquid addition pipe is connected to a container for storing culture medium, and the heating element is used to heat the culture medium in the liquid addition pipe to a specified temperature.

3. The fully automatic multifunctional workstation according to claim 1, characterized in that, The pipetting system (12) includes an electric slide rail and a plurality of lifting components disposed on the electric slide rail. The plurality of lifting components are respectively connected to the shaker (5), the liquid addition component (6), the liquid suction component (7) and the cover switch component (9).

4. The fully automatic multifunctional workstation according to claim 1, characterized in that, The switch cover assembly (9) includes a clamping member and a driving member. The clamping member is used to clamp the cover of the target carrier, and the driving member is used to drive the clamping member to perform opening and closing operations.

5. A fully automatic multifunctional workstation according to claim 1, characterized in that, The aspiration assembly (7) includes an aspiration gun disposed on one side of the pipetting system (12), and the aspiration gun is equipped with a detachable disposable pipette tip.

6. The fully automatic multifunctional workstation according to claim 1, characterized in that, The centrifugation assembly (10) includes a centrifuge disposed within the safety cabinet (2).

7. A fully automatic multifunctional workstation according to any one of claims 1-6, characterized in that, The safety cabinet (2) is located in the installation space and is also equipped with a waste disposal component (8). The waste disposal component (8) includes an installation slot opened in the safety cabinet (2), a waste collection box set in the installation slot, and an automatic door set at the opening of the waste collection box.

8. A fully automatic multifunctional workstation according to any one of claims 1-6, characterized in that, The safety cabinet (2) is equipped with a disinfection component (13) located in the installation space. The disinfection component (13) includes multiple disinfection nozzles installed in the safety cabinet (2). The multiple disinfection nozzles are used to spray disinfectant into the safety cabinet (2).

9. A fully automatic multifunctional workstation according to any one of claims 1-7, characterized in that, The safety cabinet (2) is provided with multiple consumable pre-positioning racks (11), and consumables are placed in the multiple consumable pre-positioning racks (11); The safety cabinet (2) is provided with a consumable storage component (14) on one side. The consumable storage component (14) includes a rotary switcher and multiple consumable racks arranged circumferentially along the surface of the rotary switcher.

10. A fully automatic multifunctional workstation according to any one of claims 1-7, characterized in that, A cryopreservation assembly (16) is provided on one side of the safety cabinet (2). The cryopreservation assembly (16) includes a cryopreservation shell and a cryopreservation tube installed inside the cryopreservation shell.

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