Exosome extraction device

Abstract

The utility model discloses an exosome extraction device, including main part, the top cover of setting in the top of main part, the inside of main part's top end is provided with the base, and the bottom of base and located inside main part is provided with rotor, be provided with a plurality of support on the base, the outside of base and the inside of main part are provided with limiting component, through the limiting component of design, improve the exosome extraction process in the beginning, if centrifugal tube is placed quantity, improper position or sample quantity is uneven, will lead to rotor unbalance, make centrifuge produce vibration when running, make the component wear and tear of differential centrifuge device, and there is certain influence problem to test structure, through setting limiting component between base and main part, satisfy the balance of rotor when rotating when extracting exosome to different quantity biological sample.

Description

Technical Field

[0001] This utility model belongs to the field of extraction device technology, specifically relating to an exosome extraction device. Background Technology

[0002] Exosomes, as small extracellular vesicles with a diameter of nanometers, play an important role in cell communication and epigenetic regulation. They have been extensively studied in disease diagnosis, treatment, prognosis, and monitoring of post-treatment response based on exosomes. Differential centrifugation can be used to extract exosomes.

[0003] In existing exosome extraction processes, when using differential centrifugation devices, biological samples are placed in centrifuge tubes and then into the centrifuge. If the number or position of the centrifuge tubes is improper or the sample volume is uneven, it will lead to rotor imbalance, causing the centrifuge to vibrate during operation. Uneven force on the shaft will increase the friction between the rotor and internal components of the centrifuge, such as bearings and seals, resulting in accelerated wear and shortening the centrifuge's service life. At the same time, centrifuge vibration will interfere with the normal sedimentation and separation of samples during centrifugation, preventing the effective separation of target substances such as exosomes from impurities. This will lead to reduced purity and recovery rate of the extracted exosomes, affecting the accuracy and reliability of the experiment. To address these issues, this invention proposes an exosome extraction device. Utility Model Content

[0004] The purpose of this invention is to provide an exosome extraction device to solve the problem mentioned in the background art that when the number or position of centrifuge tubes is improper or the sample volume is uneven, the differential centrifuge device will vibrate during operation, which will accelerate the wear of the components of the differential centrifuge device and affect the experiment.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an exosome extraction device, comprising a main body and a top cover disposed on the top of the main body, a base disposed on the inner side of the top of the main body, and a rotor disposed on the bottom end of the base and located inside the main body, a plurality of supports disposed on the base, a limiting component disposed on the outer side of the base and the inner side of the main body, the limiting component being composed of a locking component and a plurality of pillars, the locking component being composed of a locking groove and a locking block, the locking groove being opened on the inner side of the main body, the locking block being engaged with the inner side of the locking groove, and the plurality of pillars being disposed on the inner side of the main body.

[0006] Preferably, the surface of the card block is in contact with the inner wall of the card slot.

[0007] Preferably, the card block has a circular cross-section.

[0008] Preferably, one end of the support column is fixed to the inner side of the locking block, and the other end of the support column is fixed to the outer side of the base.

[0009] Preferably, a rolling assembly is provided at the engagement point between the card block and the card slot. The rolling assembly consists of a rolling groove, multiple balls, and multiple rolling cavities. The multiple rolling cavities are opened on the inner side of the card slot, the rolling groove is opened on the outer side of the card block, and the balls are disposed on the inner side of the rolling cavities and the inner side of the rolling groove.

[0010] Preferably, the ball has a spherical structure, and the surface of the ball is in contact with the inner wall of the rolling cavity.

[0011] Preferably, the main body has a heat dissipation vent on its side, a display screen on its front, and a knob on its front.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] By designing a limiting component, the problem of rotor imbalance caused by improper placement of centrifuge tubes or uneven sample volume during the original exosome extraction process when using a differential centrifuge device is improved. This imbalance leads to vibration of the centrifuge during operation, causing wear on the components of the differential centrifuge device and affecting the experimental structure. By setting a limiting component between the base and the main body, the rotor balance during rotation is ensured when extracting exosomes from biological samples of different quantities. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This utility model Figure 1 Enlarged schematic diagram of region A in the image;

[0016] Figure 3 This is a schematic diagram of the card block structure of this utility model;

[0017] Figure 4 This is a partial cross-sectional view of the engagement point between the limiting component and the main body of this utility model;

[0018] In the diagram: 1. Main body; 2. Top cover; 3. Base; 30. Limiting component; 301. Engaging component; 3011. Slot; 3012. Block; 302. Support; 303. Rolling component; 3031. Rolling groove; 3032. Ball; 3033. Rolling cavity; 4. Rotor; 5. Support. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figures 1 to 4 This utility model provides a technical solution: an exosome extraction device, including a main body 1, a top cover 2 disposed on the top of the main body 1, a base 3 disposed on the inner side of the top of the main body 1, and a rotor 4 disposed on the bottom end of the base 3 and located inside the main body 1. Multiple supports 5 are disposed on the base 3. A limiting component 30 is disposed on the outer side of the base 3 and the inner side of the main body 1. The limiting component 30 ensures the stability of the base 3 during rotation via the rotor 4 when testing different amounts of biological samples. The limiting component 30 consists of a locking component 301 and multiple support pillars 302. The locking component 301 consists of a slot 3011 and a locking block 3012. The slot 3011 is located inside the main body 1, and the locking block 3012 engages with the inner side of the slot 3011. The multiple support pillars 302 are disposed inside the main body 1. The designed limiting component 30 improves the performance of the differential centrifuge device during the original exosome extraction process. If the number or position of centrifuge tubes is improper or the sample volume is uneven, it will cause rotor imbalance, resulting in vibration during centrifuge operation, wear of differential centrifugation device components, and certain impact on the experimental structure. By setting a limiting component 30 between the base 3 and the main body 1, the balance of the rotor 4 during rotation is satisfied when extracting exosomes from biological samples of different quantities. The surface of the locking block 3012 is in contact with the inner wall of the locking groove 3011. The cross-section of the locking block 3012 is circular. One end of the support column 302 is fixed to the inner side of the locking block 3012, and the other end of the support column 302 is fixed to the outer side of the base 3. The locking component 301 is connected to the base 3 through the support column 302. The side of the main body 1 is provided with a heat dissipation vent, and the front of the main body 1 is provided with a display screen and a knob. The parameters of the differential centrifugation device are set through the display screen and the knob.

[0021] In this embodiment, preferably, a rolling assembly 303 is provided at the engagement point of the locking block 3012 and the locking groove 3011. The rolling assembly 303 consists of a rolling groove 3031, multiple balls 3032, and multiple rolling cavities 3033. The multiple rolling cavities 3033 are opened on the inner side of the locking groove 3011, the rolling groove 3031 is opened on the outer side of the locking block 3012, and the balls 3032 are disposed on the inner side of the rolling cavities 3033 and the inner side of the rolling groove 3031. When the locking block 3012 rotates inside the locking groove 3011 through the rolling assembly 303, the rolling assembly 303 acts as a gap between the locking groove 3011 and the locking block 3012, thereby increasing wear resistance. The balls 3032 have a spherical structure, and the surface of the balls 3032 is in contact with the inner wall of the rolling cavities 3033.

[0022] The working principle and usage process of this utility model are as follows: During the exosome extraction process, when using the differential centrifuge device, pre-centrifugation is performed first. The biological sample is placed in a centrifuge tube and fixed inside the support 5 in the main body 1. Centrifugation is performed at a low speed for 5-10 minutes. Cells, large particles, etc., due to their large mass, quickly settle to the bottom of the centrifuge tube under the action of centrifugal force. The supernatant mainly contains exosomes, smaller particulate impurities, and other soluble components. Then, centrifugation is performed at a medium speed. The supernatant after pre-centrifugation is taken and centrifuged at a higher speed for 20-30 minutes. At this time, medium-sized particulate impurities such as apoptotic bodies settle. The supernatant is further purified, and the exosomes are still retained. Finally, high-speed centrifugation is performed. The supernatant after medium-speed centrifugation is centrifuged at a higher speed for 70-120 minutes. Due to their density and size characteristics, the exosomes precipitate to the bottom of the tube under this centrifugal force. The supernatant is discarded, and the precipitate is resuspended with buffer to obtain the exosome sample.

[0023] When the differential centrifuge is working, as the base 3 rotates through the rotor 4, the support column 302 drives the locking block 3012 to rotate inside the locking slot 3011, making the base 3 more stable when rotating.

[0024] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An exosome extraction device, comprising a main body (1) and a top cover (2) disposed on the top of the main body (1), characterized in that: A base (3) is provided on the inner side of the top of the main body (1), and a rotor (4) is provided at the bottom of the base (3) and on the inner side of the main body (1). Multiple supports (5) are provided on the base (3). A limiting component (30) is provided on the outer side of the base (3) and the inner side of the main body (1). The limiting component (30) is composed of a locking component (301) and multiple pillars (302). The locking component (301) is composed of a slot (3011) and a block (3012). The slot (3011) is opened on the inner side of the main body (1), and the block (3012) is inserted into the inner side of the slot (3011). Multiple pillars (302) are provided on the inner side of the main body (1).

2. The exosome extraction device according to claim 1, characterized in that: The surface of the card block (3012) is in contact with the inner wall of the card slot (3011).

3. The exosome extraction device according to claim 2, characterized in that: The cross-section of the card block (3012) is circular.

4. The exosome extraction device according to claim 1, characterized in that: One end of the support column (302) is fixed to the inside of the locking block (3012), and the other end of the support column (302) is fixed to the outside of the base (3).

5. The exosome extraction device according to claim 1, characterized in that: A rolling assembly (303) is provided at the engagement point between the locking block (3012) and the locking groove (3011). The rolling assembly (303) consists of a rolling groove (3031), multiple balls (3032), and multiple rolling cavities (3033). The multiple rolling cavities (3033) are opened on the inner side of the locking groove (3011), the rolling groove (3031) is opened on the outer side of the locking block (3012), and the balls (3032) are located on the inner side of the rolling cavities (3033) and the inner side of the rolling groove (3031).

6. The exosome extraction device according to claim 5, characterized in that: The ball (3032) has a spherical structure, and the surface of the ball (3032) is in contact with the inner wall of the rolling cavity (3033).

7. The exosome extraction device according to claim 1, characterized in that: The main body (1) has a heat dissipation vent on its side, a display screen on its front side, and a knob on its front side.

Images