A darkroom for screening gene-edited fluorescent poultry

Abstract

This invention provides a darkroom for screening gene-edited fluorescent poultry, relating to the field of poultry gene-edited fluorescent screening equipment. It includes a darkroom assembly comprising a housing for providing observation space, a light source for illuminating the poultry, an observation window on the surface of the housing for observing the poultry inside, and an operating hole for easy handling of the poultry. A ventilation mechanism includes a fan located at the back of the housing and an air intake assembly located at the upper end of the back of the housing. This invention effectively solves the problem of insufficient ventilation in existing poultry gene-edited fluorescent screening equipment by combining the darkroom assembly and the ventilation mechanism. By setting up the air intake and exhaust assemblies, air intake and exhaust within the housing cavity are achieved respectively. Combined with the fan, air intake filter, and exhaust filter, the air inside the housing is purified, effectively reducing the concentration of odors and improving the comfort of the operating environment.

Description

Technical Field

[0001] This utility model belongs to the field of poultry gene-edited fluorescent screening box equipment, specifically a dark observation box for screening gene-edited fluorescent poultry. Background Technology

[0002] Gene-edited fluorescent poultry screening technology introduces fluorescent markers into poultry through gene editing to achieve the screening of specific targets. This method marks specific genes to assist in the selection of superior traits, effectively improving breeding efficiency. The constructed gene vector is mixed with liposomes to form a liposome-gene complex, which is then introduced into the poultry's PGC (primordial germ cells) through injection or other means. The fluorescent protein gene binds to genes or regulatory elements related to the target trait, causing the poultry to emit fluorescence under specific conditions. By observing the fluorescence expression of the PGC, PGCs that have successfully integrated GFP (green fluorescent protein) and RFP (red fluorescent protein) genes can be screened out. With the help of fluorescence signal detection, poultry individuals with the target trait can be screened quickly and accurately.

[0003] In poultry gene editing fluorescence screening, the green fluorescent protein (GFP) gene and the red fluorescent protein (RFP) gene are integrated into the poultry PGC genome. The gene-edited PGC is then injected into egg embryos. The hatched poultry are placed in a dark chamber and illuminated with a light source to cause them to fluoresce under specific conditions. The efficiency of gene editing in poultry is determined by detecting and observing the expression of fluorescent proteins in the gonads or offspring. The dark chambers used in the fluorescence screening process typically rely on natural ventilation. However, natural ventilation can be insufficient, causing odors to linger inside the chamber. During continuous use, the foul air inside the chamber cannot be expelled in time, which can easily lead to excessively high concentrations of odor gases, causing odor to spread and affecting the operating environment.

[0004] In summary, this invention provides a darkroom for screening gene-edited fluorescent poultry to solve the aforementioned problems. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0006] A darkroom for screening gene-edited fluorescent poultry includes a darkroom assembly comprising a housing for providing an observation space, a light source for illuminating the poultry, an observation window on the surface of the housing for observing the poultry inside, and an operating hole for easy handling of the poultry. A ventilation mechanism includes a fan located at the back of the housing, an air intake assembly at the upper end of the back of the housing, and an exhaust assembly at the lower end of the back of the housing. The air intake assembly includes an air intake box, an air intake slot for air intake into the inner cavity of the housing, and an air intake filter element located within the air intake box for air filtration; the air intake box is connected to the inner cavity of the housing. The exhaust assembly includes an exhaust box, an exhaust port for exhausting air from the inner cavity of the housing, an exhaust filter element located within the exhaust box, and a cover plate at the top of the exhaust box; the exhaust box is connected to the inner cavity of the housing. The air inlet of the fan is connected to the inner cavity of the exhaust box, and the air outlet of the fan is connected to an external air purifier via a pipe.

[0007] Furthermore, in this utility model, the box body includes a bottom plate, side plates located on both sides of the top of the bottom plate, a front panel located at the front end of the top of the bottom plate, a back plate located at the rear end of the top of the bottom plate, a top plate located at the top of the side plates, and a top cover hinged to the top plate by a hinge.

[0008] Furthermore, in this utility model, the side plate is fixedly connected to the bottom plate, the front panel and the back plate are both fixedly connected to the bottom plate, and both sides of the front panel and the back plate are fixedly connected to the side plates on both sides.

[0009] Furthermore, in this utility model, the light source is provided in two sets and fixed to the top of the top plate, and the irradiation end of the light source extends through the inner cavity of the box.

[0010] Furthermore, in this utility model, the observation window is located on the surface of the top cover, the operation hole is opened on the front of the panel, and a fan-shaped rubber strip is fixedly connected to the inner cavity of the operation hole.

[0011] Furthermore, in this utility model, the air intake groove is opened at the upper end of the back plate surface, the air intake box is fixed to the upper end of the back of the back plate by bolts, the air intake filter element is fixed to the inner cavity of the air intake box by bolts, and the surface of the air intake box is provided with several air intake holes.

[0012] Furthermore, in this utility model, the exhaust box is fixed to the lower end of the back of the back plate by bolts, the exhaust hole is opened at the lower end of the surface of the back plate, two sets of exhaust filter elements are provided, and both are fixed to the inner cavity of the exhaust box by bolts, and the cover plate is fixed to the top of the exhaust box by bolts.

[0013] Beneficial effects: This utility model has the following beneficial effects:

[0014] This invention effectively solves the problem of insufficient ventilation in existing poultry gene-editing fluorescent screening boxes by combining a dark box assembly and a ventilation mechanism. It avoids the retention of odors and the inability to expel polluted air in a timely manner. Furthermore, by setting up an air intake assembly and an exhaust assembly, air intake and exhaust of the inner cavity of the box are realized respectively. In conjunction with a fan, an air intake filter, and an exhaust filter, the air in the box is purified, effectively reducing the concentration of odors inside the box and improving the comfort of the operating environment. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the main structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the rear view structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the separated state of the housing and ventilation mechanism of this utility model;

[0018] Figure 4 This is a schematic diagram of the connection between the light source and the housing of this utility model.

[0019] In the picture:

[0020] 100. Darkroom assembly; 110. Cabinet body; 111. Base plate; 112. Side plate; 113. Front panel; 114. Back panel; 115. Top plate; 116. Top cover; 120. Light source; 130. Observation window; 140. Operating hole; 200. Ventilation mechanism; 210. Fan; 220. Air intake assembly; 221. Air intake box; 222. Air intake slot; 223. Air intake filter; 230. Exhaust assembly; 231. Exhaust box; 232. Exhaust hole; 233. Exhaust filter; 234. Cover plate. Detailed Implementation

[0021] To better understand the technical content of this utility model, specific embodiments are described below in conjunction with the accompanying drawings. Various aspects of this utility model are described in this disclosure with reference to the accompanying drawings, which illustrate numerous illustrative embodiments. The embodiments of this disclosure are not necessarily defined to include all aspects of this utility model. It should be understood that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed in this utility model are not limited to any particular implementation. Furthermore, some aspects of this utility model can be used alone or in any suitable combination with other aspects disclosed in this utility model.

[0022] Example 1

[0023] like Figure 1-4The image shows the first embodiment of this utility model. This embodiment provides a darkroom for screening gene-edited fluorescent poultry, including a darkroom assembly 100, comprising a housing 110 for providing observation space, a light source 120 for illuminating poultry, an observation window 130 on the surface of the housing 110 for observing poultry inside the housing, and an operating hole 140 for convenient handling of poultry. A ventilation mechanism 200 includes a fan 210 located on the back of the housing 110, an air intake assembly 220 located at the upper end of the back of the housing 110, and an exhaust assembly 230 located at the lower end of the back of the housing 110. The air intake assembly 220 includes an air intake box 2. 21. An air intake slot 222 for air intake into the inner cavity of the housing 110, and an air intake filter element 223 located in the inner cavity of the air intake box 221 for air intake filtration, and the air intake box 221 is connected to the inner cavity of the housing 110. The exhaust assembly 230 includes an exhaust box 231, an exhaust hole 232 for exhausting from the inner cavity of the housing 110, an exhaust filter element 233 located in the inner cavity of the exhaust box 231, and a cover plate 234 located on the top of the exhaust box 231, and the exhaust box 231 is connected to the inner cavity of the housing 110. The air inlet of the fan 210 is connected to the inner cavity of the exhaust box 231, and the air outlet of the fan 210 is connected to an external air purifier through a pipe.

[0024] like Figure 1-4 As shown, the fan 210 serves as the core of the ventilation power, achieving efficient air circulation within the chamber through forced airflow. Compared to natural ventilation, the fan 210 provides a stable air volume and air pressure, ensuring rapid exhaust and replenishment of air within the chamber. Air intake is achieved through natural intake, while exhaust is handled by the fan 210. The exhaust volume is higher than the intake volume, creating a negative pressure environment within the chamber, thereby reducing the spread of odors. The exhaust port of the fan 210 is connected to an external air purifier via a pipe for further deep purification of the exhaust gas. The intake filter 223 and exhaust filter 233 filter the incoming and outgoing air respectively. The intake filter 223 can be a HEPA filter, capable of filtering fine particulate matter in the air. The exhaust filter 233 has two sets, which can be either HEPA filters or activated carbon filters, capable of adsorbing odors within the chamber, thereby reducing the spread of odors.

[0025] Example 2

[0026] Reference Figure 1 , 2 3 and 4 represent the second embodiment of this utility model, which is based on the previous embodiment.

[0027] In this embodiment, the housing 110 includes a bottom plate 111, side plates 112 located on both sides of the top of the bottom plate 111, a front panel 113 located at the front end of the top of the bottom plate 111, a back plate 114 located at the rear end of the top of the bottom plate 111, a top plate 115 located at the top of the side plates 112, and a top cover 116 hinged to the top plate 115 via a hinge.

[0028] The side panel 112 is fixedly connected to the bottom plate 111, the front panel 113 and the back panel 114 are both fixedly connected to the bottom plate 111, and both sides of the front panel 113 and the back panel 114 are fixedly connected to the side panels 112 on both sides.

[0029] Two sets of light sources 120 are provided and fixed to the top of the top plate 115. The irradiation end of the light source 120 extends into the inner cavity of the housing 110.

[0030] The observation window 130 is located on the surface of the top cover 116, the operation hole 140 is opened on the front of the panel 113, and a fan-shaped rubber strip is fixedly connected to the inner cavity of the operation hole 140.

[0031] like Figure 1 , 2 As shown in Figure 3, the base plate 111 serves as the basic support structure of the box 110. The side plates 112, front panel 113, and back plate 114 constitute the four walls of the box, forming a closed space. The top plate 115 and the top cover 116 are connected by hinges for easy opening and closing. Two sets of light sources 120 on the top of the top plate 115 have their irradiation ends penetrating through the top plate 115 and entering the interior of the box. The light sources 120 can be ultraviolet or specific wavelength light sources to excite fluorescent proteins expressed by gene editing on or inside poultry. The high-brightness, specific wavelength light source effectively excites fluorescence signals, facilitating observation and screening. The observation window 130 is located on the top cover 116 and is made of a material with good light transmittance and UV protection, allowing operators to observe the fluorescence reaction on the poultry's surface through the observation window 130. The operation hole 140 is located on the front of the front panel 113, allowing operators to reach into the box to retrieve poultry. The operation hole 140 is equipped with a fan-shaped rubber strip, which not only ensures sealing during operation but also prevents external light interference and internal air leakage.

[0032] Example 3

[0033] Reference Figure 1-4 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0034] In this embodiment, the air intake groove 222 is opened at the upper end of the surface of the back plate 114, the air intake box 221 is fixed to the upper end of the back of the back plate 114 by bolts, the air intake filter element 223 is fixed to the inner cavity of the air intake box 221 by bolts, and the surface of the air intake box 221 is provided with several air intake holes.

[0035] The exhaust box 231 is fixed to the lower end of the back of the back plate 114 by bolts, the exhaust hole 232 is opened at the lower end of the surface of the back plate 114, two sets of exhaust filter elements 233 are provided, and both are fixed to the inner cavity of the exhaust box 231 by bolts, and the cover plate 234 is fixed to the top of the exhaust box 231.

[0036] like Figure 1-4 As shown, the air intake assembly 220 includes an air intake box 221, an air intake slot 222, and an air intake filter element 223. The exhaust assembly 230 includes an exhaust box 231, an exhaust port 232, an exhaust filter element 233, and a cover plate 234. By filtering the air intake and purifying the exhaust, the cleanliness of the air inside the box is maintained. External air naturally enters the air intake box 221, is filtered by the air intake filter element 223, and then enters the box through the air intake slot 222. The air inside the box is discharged through the exhaust port 232 under the action of the fan 210 and enters the exhaust box 231. After being purified by the exhaust filter element 233, the exhaust is discharged to the external air purifier through the pipe, thereby reducing the odor concentration inside the box and effectively reducing the spread of odor.

[0037] In use, the poultry to be screened is placed into the inner cavity of the box 110 through the operation port 140. The light source 120 is turned on to irradiate the fluorescent proteins on the surface or inside the poultry, causing them to emit fluorescence at a specific wavelength. The fluorescence expression is observed through the observation window 130 to determine whether the individual has been successfully gene-edited. External air enters the box through the air intake component 220 and is first purified by the air intake filter 223 to ensure that the air inside the box is clean. The air inside the box is discharged through the exhaust component 230 and then transported to an external air purifier for further purification by the fan 210, thereby maintaining the fresh air inside the box and a suitable working environment. In addition, the air intake slot 222 is located at the upper back of the box, which is conducive to the introduction of fresh air, while the exhaust port 232 is located at the lower back of the box. With the help of the fan 210, it can more effectively expel the polluted air inside the box, avoid the retention of odors and polluted air, thereby greatly reducing the odor concentration inside the box and improving the comfort of the operating environment.

[0038] All standard parts used in this application can be purchased from the market, and can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art. The control method is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art and is common knowledge in the field. Since this application is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail in this application.

[0039] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Those skilled in the art to which this invention pertains can make various modifications and refinements without departing from the spirit and scope of the present invention. Therefore, the scope of protection of this invention shall be determined by the claims.

Claims

1. A gene edited fluorescent poultry screening observation dark box, characterized in that: include, The dark box assembly (100) includes a box (110) for providing an observation space, a light source (120) for illuminating poultry, an observation window (130) located on the surface of the box (110) for observing the poultry inside the box, and an operation hole (140) for facilitating the handling of poultry. The ventilation mechanism (200) includes a fan (210) located on the back of the housing (110), an air intake assembly (220) located at the upper end of the back of the housing (110), and an exhaust assembly (230) located at the lower end of the back of the housing (110). The air intake assembly (220) includes an air intake box (221), an air intake slot (222) for air intake into the inner cavity of the housing (110), and an air intake filter element (223) located in the inner cavity of the air intake box (221) for air intake filtration. The air intake box (221) is connected to the inner cavity of the housing (110). The exhaust assembly (230) includes an exhaust box (231), an exhaust hole (232) for exhaust into the inner cavity of the housing (110), an exhaust filter element (233) located in the inner cavity of the exhaust box (231), and a cover plate (234) located on the top of the exhaust box (231). The exhaust box (231) is connected to the inner cavity of the housing (110). The air inlet of the fan (210) is connected to the inner cavity of the exhaust box (231), and the air outlet of the fan (210) is connected to an external air purifier through a pipe.

2. The dark box for observing the genetically edited fluorescent poultry for screening according to claim 1, wherein: The housing (110) includes a bottom plate (111), side plates (112) located on both sides of the top of the bottom plate (111), a front panel (113) located at the front end of the top of the bottom plate (111), a back plate (114) located at the rear end of the top of the bottom plate (111), a top plate (115) located at the top of the side plates (112), and a top cover (116) hinged to the top plate (115) by a hinge.

3. The dark box for observing the genetically edited fluorescent poultry for screening according to claim 2, characterized in that: The side plate (112) is fixedly connected to the bottom plate (111), the front panel (113) and the back plate (114) are both fixedly connected to the bottom plate (111), and both sides of the front panel (113) and the back plate (114) are fixedly connected to the side plates (112) on both sides.

4. The dark box for observing the genetically edited fluorescent poultry for screening according to claim 1, wherein: Two sets of light sources (120) are provided and fixed to the top of the top plate (115). The irradiation end of the light source (120) extends into the inner cavity of the box (110).

5. The dark box for observing the genetically edited fluorescent poultry for screening according to claim 1, characterized in that: The observation window (130) is located on the surface of the top cover (116), the operation hole (140) is opened on the front of the panel (113), and a fan-shaped rubber strip is fixedly connected to the inner cavity of the operation hole (140).

6. The dark box for observing the genetically edited fluorescent poultry for screening according to claim 1, wherein: The air intake groove (222) is opened at the upper end of the surface of the back plate (114), the air intake box (221) is fixed to the upper end of the back of the back plate (114) by bolts, the air intake filter (223) is fixed to the inner cavity of the air intake box (221) by bolts, and the surface of the air intake box (221) is provided with several air intake holes.

7. The gene edited fluorescent poultry screening viewing darkroom of claim 1 wherein: The exhaust box (231) is fixed on the lower end of the back of the back plate (114) by bolts, the exhaust hole (232) is arranged on the lower end of the surface of the back plate (114), the exhaust filter core (233) is provided with two groups, and is fixed in the inner cavity of the exhaust box (231) by bolts, and the cover plate (234) is fixed on the top of the exhaust box (231).

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