A seed nematode isolation device

Abstract

The utility model provides a kind of seed nematode separation device, it is related to nematode separation technical field, including first water storage bucket, first filter cartridge, second water storage bucket and second filter cartridge, the top of first water storage bucket has water inlet, bottom has water outlet;First filter cartridge has the first filter hole that can intercept seed and supply nematode suspension to flow out, first filter cartridge is set in first water storage bucket;Second filter cartridge is set in second water storage bucket, second filter cartridge and second water storage bucket are all located below water outlet, second filter cartridge has the second filter hole that can intercept nematode and supply water to flow out;The utility model can utilize flowing water to make the nematode in seed dissociate, and screen and collect dissociated nematode, flowing water can keep seed, water body fresh, to ensure that dissociated nematode body is fresh and live, nematode body cannot be dead, also cannot be contaminated by other parasitic fungi, to be conducive to the research of nematode in seed.

Description

Technical Field

[0001] This utility model relates to the field of nematode separation technology, and in particular to a seed nematode separation device. Background Technology

[0002] Nematodes are a collective term for animals in the phylum Nematoda. They are diverse, widely distributed, and adapted to almost all habitats. Research on nematodes is an extremely efficient and powerful way to understand the fundamental laws of life, reveal the mechanisms of human diseases, and develop new therapies. It has made, and will continue to make, immeasurable contributions to basic science and translational medicine.

[0003] Current research primarily focuses on nematodes in soil, wood, and roots, with limited study on seed-borne nematodes. This is because seed-borne nematode populations are low, and the nematodes are in a dormant state, inactive, and slow to migrate from the seed, making separation difficult. However, seed-borne nematodes can still pose a threat to seed and plant development.

[0004] Traditional nematode isolation devices typically use prolonged immersion in water to separate nematodes from soil, wood, and roots. However, prolonged soaking of seeds can lead to rotting, bacterial growth, and oxygen depletion in the water, causing nematode death. Dead nematodes exhibit blurred structures, affecting morphological identification; furthermore, the nucleic acids of dead nematodes are lysed, hindering subsequent PCR amplification, or the nematodes may be infected by parasitic bacteria or fungi, affecting the purity of the nucleic acid template and leading to molecular detection failure. Utility Model Content

[0005] The purpose of this invention is to provide a seed nematode separation device to solve the problems existing in the prior art. It can use flowing water to release nematodes from seeds and then screen and collect the released nematodes. The flowing water can keep the seeds and water fresh, thus ensuring that the released nematodes are alive and do not die or become contaminated by other parasitic bacteria, which is beneficial for the study of nematodes in seeds.

[0006] To achieve the above objectives, this utility model provides the following solution:

[0007] A seed-nematode separation device includes a first water storage tank, a first filter cartridge, a second water storage tank, and a second filter cartridge. The first water storage tank has a water inlet at the top and a water outlet at the bottom. The first filter cartridge is used to hold seeds containing nematodes and has first filter holes capable of intercepting seeds and allowing a nematode suspension to flow out. The first filter cartridge is disposed in the first water storage tank, and the water level in the first water storage tank is higher than the seed spreading surface but does not exceed the top of the first filter cartridge. The second filter cartridge is disposed in the second water storage tank, and both the second filter cartridge and the second water storage tank are located below the water outlet. The second filter cartridge has second filter holes capable of intercepting nematodes and allowing water to flow out.

[0008] In one embodiment, the top of the first filter cartridge is higher than the first water storage tank.

[0009] In one embodiment, the top of the second filter cartridge is higher than the second water storage tank.

[0010] As one embodiment, the first water storage tank has a bracket for placing the first filter cartridge.

[0011] As one embodiment, the bottom of the first water storage tank is a conical structure with a diameter that gradually decreases from bottom to top, and the bottom of the conical structure has the water outlet.

[0012] As one implementation, a valve is provided at the water outlet.

[0013] As one embodiment, it also includes a spraying mechanism, wherein the spraying head of the spraying mechanism is located above the first water storage tank.

[0014] In one embodiment, the valve is an electrically controlled valve, and the second water storage tank is equipped with a level gauge for monitoring its liquid level. The level gauge is communicatively connected to the electrically controlled valve and the spray mechanism.

[0015] As one embodiment, the second filter cartridge is provided with a support arm for supporting the side wall of the second water storage tank.

[0016] As one embodiment, the pore size of the first filter pore is 0.2 mm to 1 mm, and the pore size of the second filter pore is 0.02 mm to 0.05 mm.

[0017] This utility model has the following technical advantages over the prior art:

[0018] The seed nematode separation device of this invention can use flowing water to release nematodes from seeds and then sieve and collect the released nematodes. The flowing water keeps the seeds and water fresh, thus ensuring that the released nematodes are alive and do not die or become contaminated by other parasitic bacteria. This facilitates the study of nematodes in seeds and provides a theoretical basis for crop cultivation. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the structure of a seed nematode separation device in one embodiment of the present invention;

[0021] Figure 2 for Figure 1 Top view;

[0022] Figure 3 for Figure 1 A schematic diagram of the side section structure;

[0023] Figure 4 This is an exploded view of the seed nematode separation device in one embodiment of the present invention;

[0024] Figure 5 This is a schematic diagram of the internal structure of the first water storage tank in one embodiment of the present invention.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. First water storage tank; 2. First filter cartridge; 3. Second water storage tank; 4. Second filter cartridge; 5. Mounting frame; 6. Water outlet; 7. Bracket; 8. Support arm; 9. Spray head. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] The purpose of this invention is to provide a seed nematode separation device to solve the problems existing in the prior art. It can use flowing water to release nematodes from seeds and then screen and collect the released nematodes. The flowing water can keep the seeds and water fresh, thus ensuring that the released nematodes are alive and do not die or become contaminated by other parasitic bacteria, which is beneficial for the study of nematodes in seeds.

[0029] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] like Figures 1-5 As shown, this embodiment provides a seed-nematode separation device, including a first water storage tank 1, a first filter cartridge 2, a second water storage tank 3, and a second filter cartridge 4. The first water storage tank 1 is installed at a higher position relative to the second water storage tank 3; for example, the first water storage tank 1 can be installed on a mounting frame 5. The first water storage tank 1 has a water inlet at the top and a water outlet 6 at the bottom. Specifically, the top of the first water storage tank 1 can be an open structure, serving as a water inlet for direct water injection, which facilitates both water injection and the removal and placement of the first filter cartridge 2. The first filter cartridge 2 is used to hold seeds containing nematodes and has first filter holes capable of intercepting seeds and allowing a nematode suspension (a solution mixed with nematodes) to flow out. The pore size of the first filter holes can be 0.2 mm to 1 mm; specifically, the size of the first filter holes can be reasonably designed according to different seeds. The first filter cartridge 2 is placed inside the first water storage tank 1. The water level in the first water storage tank 1 is higher than the surface of the seeds to ensure that the seeds in the first filter cartridge 2 can be soaked. However, the water level in the first water storage tank 1 does not exceed the top of the first filter cartridge 2. This is because, in the initial stage of watering, the seeds are relatively dry, and the density of the seeds may vary. Some seeds may float on the water surface (the buoyancy of seeds changes over time; most seeds become denser than water, so some of the initially floating seeds will sink to the bottom of the first filter cartridge 2). If the water level in the first water storage tank 1 is higher than the top of the first filter cartridge 2, the floating seeds will flow out of the first filter cartridge 2, resulting in a shorter soaking time for these seeds. Nematodes will not have enough time to escape from the seeds, thus reducing the number of nematodes collected. The second filter cartridge 4 is installed inside the second water storage tank 3. Both the second filter cartridge 4 and the second water storage tank 3 are located below the water outlet 6. The bottom of the second filter cartridge 4 has a second filter hole that can intercept nematodes and supply water. The diameter of the second filter hole is 0.02mm to 0.05mm. Specifically, the size of the second filter hole can be reasonably designed according to the size of different types of nematodes.

[0031] In use, first seal the outlet 6, pour a certain amount of water into the first water storage tank 1 to submerge the seeds, then open the outlet 6 and continuously pour water into the first water storage tank 1 to maintain a balance between water inflow and outflow, ensuring that the water level in the first water storage tank 1 always submerges the seeds. The water in the first water storage tank 1 flows into the second filter cartridge 4, which filters out the nematodes in the water for subsequent research. The filtered water is collected by the second water storage tank 3. Initially, the amount of nematodes collected by the second filter cartridge 4 may be small, but over time, the number of nematodes filtered from the soaking water (the water that has soaked the seeds) will increase. Of course, in use, you can also seal the outlet 6, soak the seeds for a short time (control the soaking time to avoid prolonged soaking and rotting), and then open the outlet 6 to allow the flowing water to release the nematodes from the seeds.

[0032] Therefore, the seed nematode separation device in this embodiment can use flowing water to release nematodes from the seeds and then sieve and collect the released nematodes. The flowing water can keep the seeds and water fresh, thus ensuring that the released nematodes are alive and do not die or become contaminated by other parasitic bacteria. This is beneficial for studying nematodes in seeds and provides a theoretical basis for crop cultivation.

[0033] In order to ensure that the water level in the first water storage tank 1 does not exceed the top of the first filter cylinder 2, in this embodiment the top of the first filter cylinder 2 is higher than the first water storage tank 1.

[0034] During use, the bottom of the second filter cartridge 4 should be close to the bottom of the second water storage tank 3 to ensure that the bottom of the second filter cartridge 4 is submerged in water for most of the nematode separation process, preventing prolonged drying of the bottom area of ​​the second filter cartridge 4 and the resulting inactivation of nematodes in this dry area. In this embodiment, the top of the second filter cartridge 4 is higher than the second water storage tank 3 to prevent nematodes in the second filter cartridge 4 from flowing into the second water storage tank 3.

[0035] like Figure 5 As shown, in this embodiment, the first water storage tank 1 has a bracket 7 for placing the first filter cartridge 2. The bracket 7 in this embodiment is cross-shaped and can support the first filter cartridge 2. In order to ensure the installation stability of the first filter cartridge 2, the bracket 7 and the first filter cartridge 2 can be connected by a connecting structure, such as a rope or bolt.

[0036] In this embodiment, the first filter cartridge 2 and the second filter cartridge 4 can be rigid filter screens. Since the first filter cartridge 2 is used to hold seeds, it can use a flexible filter screen or filter cloth. For example, the first filter cartridge 2 may include a metal frame and a filter screen (or filter cloth). The metal frame may include two metal rings at the top and bottom and a connecting rod connecting the two metal rings. Fixing the pocket-shaped filter screen (or filter cloth) to the top metal ring forms the first filter cartridge 2.

[0037] Nematodes are usually denser than water and sink to the bottom. In order to ensure the number of nematodes collected, the bottom of the first water storage tank 1 in this embodiment is a conical structure with a diameter that gradually decreases from bottom to top. The bottom of the conical structure has a water outlet 6, which is beneficial to the slope of the conical structure so that the water flow can flush all the nematodes into the second filter cartridge 4.

[0038] In this embodiment, valves are installed at the 6 water outlets. The valves can be electrically controlled valves and have flow regulation functions.

[0039] like Figure 3 As shown, this embodiment also includes a spraying mechanism, which may include a third water storage tank, a lift pump submerged in the third water storage tank, and a spray head 9 connected to the outlet 6 of the lift pump. The spray head 9 is located above the first water storage tank 1 and can spray water into the first water storage tank 1 and the first filter cartridge 2.

[0040] In this embodiment, the second water storage tank 3 is equipped with a level gauge for monitoring its liquid level. The level gauge is connected to the electric control valve and the lift pump in the spray mechanism. When the water level in the second water storage tank 3 reaches the set value, the electric control valve and the lift pump are turned off, thereby realizing the automatic shutdown of water supply without the need for staff to monitor the entire separation process for a long time.

[0041] In this embodiment, the second filter cartridge 4 is provided with a support arm 8 for supporting the side wall of the second water storage tank 3. The support arm 8 is in the shape of a hook bent downwards, which can be hung on the top of the side wall of the second water storage tank 3, making it convenient to disassemble and assemble the second filter cartridge 4.

[0042] In this embodiment, both the first water storage tank 1 and the second water storage tank 3 are made of stainless steel with smooth inner walls, which makes them easy to clean and dry, and avoids contamination of the isolation and detection of nematodes in other seeds.

[0043] Any adaptive changes made according to actual needs are within the protection scope of this utility model.

[0044] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A seed nematode separation device, characterized in that, include: The first water storage tank has a water inlet at the top and a water outlet at the bottom; A first filter cartridge is used to hold seeds containing nematodes. The first filter cartridge has a first filter hole that can intercept the seeds and allow the nematode suspension to flow out. The first filter cartridge is disposed in a first water storage tank. The water level in the first water storage tank is higher than the seed spreading surface and does not exceed the top of the first filter cartridge. The second water storage tank and the second filter cartridge are arranged inside the second water storage tank. Both the second filter cartridge and the second water storage tank are located below the water outlet. The second filter cartridge has a second filter hole that can intercept nematodes and supply water to flow out.

2. The seed nematode separation device according to claim 1, characterized in that, The top of the first filter cartridge is higher than the first water storage tank.

3. The seed nematode separation device according to claim 1, characterized in that, The top of the second filter cartridge is higher than the second water storage tank.

4. The seed nematode separation device according to claim 1, characterized in that, The first water storage tank has a bracket for placing the first filter cartridge.

5. The seed nematode separation device according to claim 4, characterized in that, The bottom of the first water storage tank is a conical structure with a diameter that gradually decreases from bottom to top, and the bottom of the conical structure has the water outlet.

6. The seed nematode separation device according to claim 5, characterized in that, A valve is installed at the water outlet.

7. The seed nematode separation device according to claim 6, characterized in that, It also includes a spraying mechanism, in which the spray head is located above the first water storage tank.

8. The seed nematode separation device according to claim 7, characterized in that, The valve is an electrically controlled valve, and the second water storage tank is equipped with a level gauge for monitoring the liquid level. The level gauge is communicatively connected to the electrically controlled valve and the spray mechanism.

9. The seed nematode separation device according to claim 3, characterized in that, The second filter cartridge is provided with a support arm for supporting the side wall of the second water storage tank.

10. The seed nematode separation device according to claim 1, characterized in that, The diameter of the first filter pore is 0.2 mm to 1 mm, and the diameter of the second filter pore is 0.02 mm to 0.05 mm.

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