A sample grinding and lysing device and detection device

Abstract

This utility model discloses a sample grinding and lysis device and a detection device; the lysis device includes a grinding assembly and a lysis cup. The grinding assembly includes a lower grinding cup, an upper grinding cup, and a grinding head. The grinding head is fixed inside the grinding chamber of the lower grinding cup. The lower grinding cup includes a main body and a grinding sleeve. The main body is coaxially rotatably connected to the upper grinding cup. The grinding sleeve is fixed inside the main body and surrounds the grinding head. The sample grinding and lysis device provided by this utility model fixes the grinding head and grinding sleeve to the coaxially rotatably connected lower and upper grinding cups, respectively, so that sample grinding can be achieved by manually rotating the upper grinding cup, which is convenient to operate and suitable for outdoor use. This utility model places the lysis cup below the grinding chamber and connects it with multiple inlet holes, so that the ground sample particles automatically enter the lysis solution for lysis, making the operation very convenient.

Description

Technical Field

[0001] This utility model belongs to the field of solid sample detection technology, specifically relating to a sample grinding and pyrolysis device and a detection device. Background Technology

[0002] In the field of biological sample testing, especially in rapid outdoor testing scenarios, pretreatment of solid samples with high fiber content, such as plant leaves and fungal fruiting bodies, is often required. Taking the on-site detection of poisonous mushrooms as an example, the mushrooms need to be ground into fine fragments before being fully contacted with lysis buffer to release the target toxin molecules (such as amatoxins). Traditional procedures have significant drawbacks: first, the sample is physically broken up in a mortar using a hand-held pestle; then, the ground product is added to the lysis buffer with a scraper and shaken to mix; finally, the supernatant is aspirated with a pipette for detection. This process involves multiple different instruments, is cumbersome, and cannot meet the needs of rapid outdoor testing. Summary of the Invention

[0003] The purpose of this invention is to provide a sample grinding and pyrolysis device and a detection device.

[0004] In a first aspect, this utility model provides a sample grinding and lysis device, which includes a grinding assembly and a lysis cup. The grinding assembly includes a lower grinding cup, an upper grinding cup, and a grinding head. A partition is provided in the inner cavity of the lower grinding cup. The partition divides the inner cavity of the lower grinding cup into a grinding chamber and a connecting chamber. The top opening of the lysis cup is connected to the connecting chamber. The grinding head is fixed in the grinding chamber. The upper grinding cup includes a main body and a grinding sleeve. The main body is coaxially rotatably connected to the lower grinding cup. The grinding sleeve is fixed inside the main body. The grinding sleeve extends into the lower grinding cup and surrounds the grinding head. A grinding groove is formed between the grinding sleeve and the grinding head. The grinding chamber communicates with the inner cavity of the lysis cup through an outlet hole.

[0005] Preferably, the outer peripheral surface of the grinding head is provided with a plurality of first grinding teeth. The inner wall of the grinding sleeve is provided with a plurality of second grinding teeth. The first grinding teeth and the second grinding teeth are oriented in opposite directions.

[0006] Preferably, a screw is fixed to the bottom of the grinding head. The screw passes through the central through hole of the partition and is fixed by a nut. The orientation of the first grinding tooth on the grinding head is opposite to the direction in which the grinding head is tightened relative to the nut.

[0007] Preferably, the outer peripheral surface of the grinding head and the inner wall of the grinding sleeve are both frustum-shaped, and their diameters gradually decrease along the direction away from the partition. The cone angle of the inner wall of the grinding sleeve is smaller than the cone angle of the grinding head.

[0008] Preferably, the top of the lower grinding cup is provided with an annular groove; the bottom of the main body of the upper grinding cup is provided with an annular protrusion. The annular protrusion and the annular groove cooperate to form a rotatable connection.

[0009] Preferably, the grinding assembly further includes a grinding cover. The grinding cover is installed at the top opening of the grinding chamber.

[0010] Preferably, the pyrolysis cup body and the lower grinding cup body are connected by a threaded connection.

[0011] Preferably, the outlet hole is formed on the outer peripheral surface of the grinding head and extends through the grinding head and the partition.

[0012] Secondly, this utility model provides a detection device, which includes a detection component and the aforementioned sample grinding and lysis device. The detection component includes a detection cup, a dual-purpose cap, and reagent strips. The top of the detection cup has an opening. One or more reagent strips are provided on the inner sidewall of the detection cup. The dual-purpose cap is detachably connected to the top opening of the detection cup.

[0013] Preferably, the dual-purpose cap has a drip tip structure in the middle. The top opening of the detection cup has the same structure as the top opening of the pyrolysis cup, allowing the dual-purpose cap to be connected to the pyrolysis cup.

[0014] The present invention has the following beneficial effects.

[0015] 1. The sample grinding and lysis device provided by this utility model fixes the grinding head and grinding sleeve on the lower grinding cup and the upper grinding cup, which are coaxially rotatably connected, respectively. Thus, the grinding of the sample can be achieved by manually rotating the upper grinding cup. It is convenient to operate and suitable for outdoor operation.

[0016] 2. This utility model places the lysis cup below the grinding chamber and connects it with multiple inlet holes, so that the sample particles obtained from grinding automatically enter the lysis solution for lysis, making the operation very convenient.

[0017] 3. This utility model allows for convenient addition of lysis solution containing the sample to the detection cup by removing the lysis cup and installing a dual-purpose cap with a dropper structure, thus achieving rapid and accurate detection. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the exploded structure of the sample grinding and pyrolysis device provided in Example 1.

[0019] Figure 2 This is a schematic diagram of the internal structure of the sample grinding and pyrolysis device provided in Example 1.

[0020] Figure 3This is a schematic diagram of the exploded structure of the detection component in Example 2.

[0021] Reference numerals in the attached diagram: 1. Pyrolysis cup; 2. Lower grinding cup; 2-1. Partition plate; 2-2. Nut; 3. Upper grinding cup; 3-1. Main body; 3-2. Grinding sleeve; 3-3. Annular protrusion; 4. Grinding head; 4-1. Outlet hole; 5. Grinding cover; 6. Grinding groove; 7. Detection cup; 8. Dual-purpose cover; 9. Mounting piece; 10. Reagent strip; 11. Sealing cap. Detailed Implementation

[0022] The technical solution of this utility model will be further described in detail below with reference to the accompanying drawings.

[0023] Example 1

[0024] like Figure 1 and Figure 2 As shown, a sample grinding and lysis device includes a grinding assembly and a lysis cup 1 installed at the bottom of the grinding assembly. The grinding assembly includes a lower grinding cup 2, an upper grinding cup 3, a grinding head 4, and a grinding cover 5. The lower grinding cup 2 has an open structure at both the top and bottom, and its inner cavity is integrally formed with a partition 2-1. The partition 2-1 is located in the lower middle part of the inner cavity of the lower grinding cup 2, dividing the inner cavity of the lower grinding cup 2 into an upper grinding cavity and a lower connecting cavity. The grinding cover 5 is installed on the top opening of the grinding cavity and can be opened or removed.

[0025] The pyrolysis cup 1 and the lower grinding cup 2 are detachably connected via a connecting cavity. In some embodiments, the inner wall of the connecting cavity is provided with an internal thread. The top of the pyrolysis cup 1 is provided with an external thread. The pyrolysis cup 1 and the connecting cavity of the lower grinding cup 2 are threadedly connected.

[0026] The grinding head 4 is coaxially fixed to the top surface of the partition 2-1 in the lower grinding cup body 2. In this embodiment, a screw is integrally formed at the bottom center of the grinding head 4. The screw passes through the central through hole of the partition 2-1 from top to bottom and is connected to a nut 2-2. The nut 2-2 abuts against the bottom surface of the partition 2-1, thereby fixing the grinding head 4 and the lower grinding cup body 2 together. In some embodiments, the nut 2-2 is bonded or welded to the partition 2-1. In other embodiments, the nut 2-2 and the partition 2-1 are integrally formed, and the nut 2-2 is formed by machining threads in the central hole of the partition 2-1. The grinding head 4 is frustum-shaped, and its diameter gradually decreases along the direction away from the partition 2-1. The outer circumferential surface of the grinding head 4 is provided with first grinding teeth.

[0027] The upper grinding cup 3 includes a main body 3-1 and a grinding sleeve 3-2. The main body 3-1 and the grinding sleeve 3-2 can be either an integral structure or a separate, fixed structure. The bottom edge of the main body 3-1 is rotatably connected to the top edge of the lower grinding cup 2. The upper grinding cup 3 and the lower grinding cup 2 are coaxially arranged. The main body 3-1 is a hollow, rotating body. The grinding sleeve 3-2 has a hollow structure. The top edge of the grinding sleeve 3-2 is integrally connected to the inner wall of the main body 3-1. The grinding sleeve 3-2 extends downwards into the lower grinding cup 2 and surrounds the grinding head 4. An anti-slip sleeve is provided on the outer side of the main body 3-1.

[0028] The inner wall of the grinding sleeve 3-2 is frustum-shaped, and its diameter gradually decreases in the direction away from the partition 2-1. The bottom edge of the inner wall of the grinding sleeve 3-2 is close to the bottom edge of the grinding head 4. In some further embodiments, the gap between the bottom edge of the inner wall of the grinding sleeve 3-2 and the bottom edge of the grinding head 4 is as small as possible to avoid interference between the grinding sleeve 3-2 and the grinding head 4 during rotation, so as to ensure the grinding effect.

[0029] The cone angle of the inner wall of the grinding sleeve 3-2 is smaller than that of the grinding head 4, resulting in a grinding groove 6 between the grinding sleeve 3-2 and the grinding head 4, which gradually narrows in width along the direction close to the partition 2-1. A second grinding tooth is provided on the frustum-shaped inner wall of the grinding sleeve 3-2. The first grinding tooth on the grinding head 4 and the second grinding tooth on the grinding sleeve 3-2 are oriented oppositely, specifically, their circumferential components along the axis of the grinding head 4 are opposite.

[0030] In some embodiments, the top of the outer surface of the lower grinding cup 2 is provided with an annular groove. The bottom edge of the main body portion 3-1 of the upper grinding cup 3 is provided with an inwardly protruding annular convex edge 3-3. The annular convex edge 3-3 engages with the annular groove to form a rotatable connection. The annular convex edge 3-3 and the annular groove make the upper grinding cup 3 and the lower grinding cup 2 form a rotating pair. The rotation axis of the rotating pair coincides with the axis of the upper grinding cup 3 and the lower grinding cup 2.

[0031] The orientation of the first grinding tooth on the grinding head 4 (specifically, the circumferential component) is opposite to the tightening direction of the grinding head 4 relative to the nut 2-2. This ensures that the direction of the torque on the grinding head 4 during the grinding process is consistent with the tightening direction of the grinding head 4, thus preventing the grinding head 4 from being loosened during the grinding process.

[0032] In some embodiments, the grinding head 4 and the partition 2-1 are provided with a plurality of outlet holes 4-1. The outlet holes 4-1 penetrate the grinding head 4 and the partition 2-1, allowing sample debris with a particle size smaller than the diameter of the outlet holes 4-1 to leave the grinding chamber and enter the lysis cup 1. The axis of the outlet holes 4-1 is parallel to the axis of the grinding head 4. The outer circumferential surface of the grinding head 4 is provided with multiple rings of outlet holes 4-1. Each ring of outlet holes 4-1 includes a plurality of outlet holes 4-1 arranged along the circumference of the grinding head 4.

[0033] In some other embodiments, the partition 2-1 is provided with a plurality of outlet holes 4-1. The outlet holes 4-1 are located in the gap between the grinding head 4 and the bottom of the grinding sleeve 3-2, and are used to export the sample debris obtained from grinding out of the grinding chamber.

[0034] In some other embodiments, grinding beads are placed in the grinding groove 6. The grinding head 4, grinding sleeve 3-2, and grinding beads can be made of ceramic material, while other accessories can be made of high-strength plastics such as PET and PP.

[0035] The working principle of this embodiment is as follows:

[0036] Open the grinding cover 5 and put the sample to be processed (e.g., mushrooms that need to be tested for toxicity; the mushrooms can be chopped before being put in to increase the grinding speed) into the grinding chamber.

[0037] Cover the grinding chamber with the grinding cap 5, and manually rotate the lower grinding cup 2 relative to the upper grinding cup 3. The first and second grinding teeth move relative to each other, grinding the sample in the grinding chamber into fragments. The fragments enter the lysis cup containing lysis buffer through the outlet hole 4-1 for lysis for 3-5 minutes. The increased contact area between the fragmented sample and the lysis buffer accelerates the release of small molecules and improves lysis efficiency.

[0038] In some optional embodiments, the lysis buffer is an organic solvent and an inorganic solvent in a certain ratio (for example, the lysis buffer includes 10% to 20% methanol by mass, 6.057 g / L to 12 g / L Tris by mass, 6 to 8 g / L NaCl by mass, 2 g / L to 5 g / L Tween 20 by mass, 0.02% Proclin 300 by mass, and the remainder is pure water), and the lysis buffer yields the lysis product test solution.

[0039] Example 2

[0040] like Figure 3 As shown, a solid sample lysis detection device includes a sample grinding and lysis apparatus and a detection component. The sample grinding and lysis apparatus is the same as the sample grinding and lysis apparatus provided in Example 1.

[0041] The detection assembly includes a detection cup 7, a dual-purpose cover 8, a mounting plate 9, and reagent strips 10. The top of the detection cup 7 has an opening. The dual-purpose cover 8 is mounted on the detection cup 7 and can be removed. The mounting plate 9 is disposed on the inner wall of the detection cup 7. The reagent strips 10 are arranged sequentially on the mounting plate 9.

[0042] In some embodiments, the mounting piece 9 is a blister pack.

[0043] In some embodiments, the dual-purpose cover 8 is threadedly connected to the detection cup 7. The detection cup 7 has the same thread as the top of the lysis cup. The dual-purpose cover 8 can be installed onto the lysis cup. The dual-purpose cover 8 has a dropper structure in its middle. The dropper structure has a dropper hole. A sealing cap 11 for sealing the dropper hole can be detachably installed on the dropper structure. By installing the detection cup 7 onto the lysis cup, the lysis solution containing the sample can be added to the detection cup 7 for detection.

[0044] The working principle of this embodiment is as follows:

[0045] The sample is ground and lysed using a sample grinding and lysis device. The lysate containing the sample is then added to the test cup 7. The reagent strip 10 is used to test the sample, and the test results are observed after a preset time.

[0046] In some optional embodiments, the reagent strip 10 includes a cyprotinin colloidal gold detection reagent strip 10, an amatoxins colloidal gold detection reagent strip 10, and a bufotenine colloidal gold immunochromatographic reagent strip 10, corresponding to the detection analytes cyprotinin, amatoxins (including α-amatoxins, β-amatoxins, and γ-amatoxins), and bufotenine, respectively. The detection principle is a competitive method, with the C line (control line) as the quality control line and the T line (test line) as the detection line; the appearance of only the C line indicates a positive result, the appearance of both the C line and the T line indicates a negative result, and the absence of the C line indicates an invalid result.

[0047] In some embodiments, the sample being tested is a mushroom. This embodiment uses fresh mushrooms, dried mushrooms, or cooked mushrooms that have been ground, pulverized, or pyrolyzed before testing.

Claims

1. A sample grinding and lysis apparatus, comprising a grinding assembly and a lysis cup (1); characterized in that: The grinding assembly includes a lower grinding cup (2), an upper grinding cup (3), and a grinding head (4); the lower grinding cup (2) has a partition (2-1) in its inner cavity; the partition (2-1) divides the inner cavity of the lower grinding cup (2) into a grinding cavity and a connecting cavity; the top opening of the pyrolysis cup (1) is connected to the connecting cavity; the grinding head (4) is fixed in the grinding cavity; the upper grinding cup (3) includes a main body (3-1) and a grinding sleeve (3-2); the main body (3-1) is coaxially rotatably connected to the lower grinding cup (2); the grinding sleeve (3-2) is fixed inside the main body (3-1); the grinding sleeve (3-2) extends into the lower grinding cup (2) and surrounds the grinding head (4); a grinding groove (6) is formed between the grinding sleeve (3-2) and the grinding head (4); the grinding cavity is connected to the inner cavity of the pyrolysis cup (1) through an outlet hole.

2. The sample grinding and lysis apparatus according to claim 1, characterized in that: The grinding head (4) has a plurality of first grinding teeth on its outer peripheral surface; the grinding sleeve (3-2) has a plurality of second grinding teeth on its inner wall; the first grinding teeth and the second grinding teeth are oriented in opposite directions.

3. The sample grinding and lysis apparatus according to claim 2, characterized in that: The bottom of the grinding head (4) is fixed with a screw; the screw passes through the central through hole of the partition (2-1) and is fixed by a nut; the orientation of the first grinding tooth on the grinding head (4) is opposite to the direction in which the grinding head (4) is tightened relative to the nut (2-2).

4. The sample grinding and pyrolysis apparatus according to claim 1, characterized in that: The outer circumferential surface of the grinding head (4) and the inner wall of the grinding sleeve (3-2) are both frustum-shaped, and their diameters gradually decrease along the direction away from the partition (2-1); the cone angle of the inner wall of the grinding sleeve (3-2) is smaller than the cone angle of the grinding head (4).

5. The sample grinding and lysis apparatus according to claim 1, characterized in that: The lower grinding cup (2) is provided with an annular groove at the top; the upper grinding cup (3) is provided with an annular protrusion (3-3) at the bottom of the main body (3-1); the annular protrusion (3-3) cooperates with the annular groove to form a rotatable connection.

6. The sample grinding and lysis apparatus according to claim 1, characterized in that: The grinding assembly also includes a grinding cover (5); the grinding cover (5) is installed at the top opening of the grinding chamber.

7. The sample grinding and lysis apparatus according to claim 1, characterized in that: The pyrolysis cup (1) is threadedly connected to the connecting cavity of the lower grinding cup (2).

8. The sample grinding and lysis apparatus according to claim 1, characterized in that: The outlet hole is opened on the outer peripheral surface of the grinding head (4) and penetrates the grinding head (4) and the partition (2-1).

9. A detection device, characterized in that: The device includes a detection component and a sample grinding and lysis apparatus as described in claim 1; the detection component includes a detection cup (7), a dual-purpose cap (8), and reagent strips (10); the top of the detection cup (7) is provided with an opening; one or more reagent strips (10) are provided on the inner sidewall of the detection cup (7); the dual-purpose cap (8) is detachably connected to the top opening of the detection cup (7).

10. A detection device according to claim 9, characterized in that: The dual-purpose cover (8) is provided with a drip head structure in the middle; the top opening of the detection cup (7) is the same as the top opening structure of the pyrolysis cup (1).

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