Methods and compositions for assessing RNA levels in biological samples
EP4751097A1Pending Publication Date: 2026-06-03DOT LABORATORIES INC
Patent Information
- Authority / Receiving Office
- EP · EP
- Patent Type
- Applications
- Current Assignee / Owner
- DOT LABORATORIES INC
- Filing Date
- 2024-07-24
- Publication Date
- 2026-06-03
AI Technical Summary
Technical Problem
There is an unmet need for accurately measuring microRNAs (miRNAs) and understanding their stability in minimally invasive patient samples like blood, as degraded RNA can interfere with identifying biomarkers and producing reliable diagnostics.
Method used
The methods involve extracting RNA from biological samples such as saliva, serum, or plasma within specific time frames (e.g., no more than 50 days, 30 days, 7 days, or 1 day) from collection to minimize RNA degradation, and using classifiers developed from these samples for diagnostic purposes.
Benefits of technology
This approach ensures a more uniform state of RNA in samples, reducing degradation and enhancing the accuracy of miRNA measurements, which can lead to reliable diagnostic and therapeutic decisions.
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Figure US2024039408_30012025_PF_FP_ABST
Abstract
This disclosure provides methods for assessing miRNA levels in bodily samples involve extracting RNA from the samples no more than 50 days after sample collection. This disclosure further provides methods of creating sample training sets in which RNA degradation is minimized. The methods involve extracting RNA from a biological sample, such as saliva, serum or plasma, in no more than 50 days from collection of the sample, for example, no more than 30 days, 110 more than seven days or no more than 1 day. This produces a collection of samples that is more uniform in the state of RNA present.
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