Modified firefly luciferase, preparation method therefor and use thereof

Modified firefly luciferase mutants with specific amino acid changes enhance enzyme activity and thermal stability, addressing the limitations of existing technologies for improved ATP detection and cell viability assays.

EP4756016A1Pending Publication Date: 2026-06-10BEYOTIME BIOTECH INC
View PDF 1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
EP · EP
Patent Type
Applications
Current Assignee / Owner
BEYOTIME BIOTECH INC
Filing Date
2024-07-19
Publication Date
2026-06-10

Smart Images

  • Figure IMGF0001
    Figure IMGF0001
  • Figure IMGF0002
    Figure IMGF0002
  • Figure IMGF0003
    Figure IMGF0003
Patent Text Reader

Abstract

Provided in the present invention are a series of substrate binding pocket-modified firefly luciferases having high enzyme activity and high thermal stability, a preparation method therefor and the use thereof. Through the analysis of the spatial structure of the substrate binding pocket of the firefly luciferase, and on the basis of considering a large number of amino acid sites related to enzyme activity and thermal stability of firefly luciferase, the present inventors design, construct and detect a series of firefly luciferase mutants. The firefly luciferase has a high enzyme activity and thermal stability.
Need to check novelty before this filing date? Find Prior Art