Composite polyester fiber, polyester fiber cloth, fiber cloth carrier, and preparation method and use

Abstract

A composite polyester fiber, a polyester fiber fabric comprising the composite polyester fiber, a fiber fabric carrier, a cell culture carrier, and preparation methods and uses thereof. The composite polyester fiber has a core-sheath structure; wherein the mass ratio of the core material to the sheath material is x, and 1.5 ≤ x ≤ 4; and the average diameter D of the composite polyester fiber is 10-40 µm. The polyester fiber fabric, the fiber fabric carrier or cell culture carrier made of the composite polyester fiber can improve the sugar metabolism capacity of cells when they are used for cell culture.

Description

Technical Field

[0001] The present invention relates to the technical field of composite polyester fibers, specifically to a composite polyester fiber, a polyester fiber fabric made of the composite polyester, a fiber fabric carrier, and preparation methods and uses thereof.Prior Art

[0002] Currently, large-scale animal cell culture technology is widely used in the production of various cells and cell products, including biological products such as enzymes, growth factors, vaccines, antibodies and the like. Most animal cells have the habit of anchorage-dependent growth, and therefore, during in vitro culture, it is usually required to provide a support for their growth, and such a support is commonly referred to by those skilled in the art as a cell culture carrier. Current cell culture carriers include particulate carriers with a diameter of 2-11 mm, porous spherical carriers, or sheet-like fiber carriers. Current microcarriers include silk fibroin and chitosan macroporous microcarriers, mixed chitosan and gelatin microcarriers and the like. Spherical microcarriers have the problems that cells can only adhere to the surface and the area-to-volume ratio is small, and although porous microcarriers have pores that facilitate cell adhesion, their pores are prone to blockage, which in turn impairs oxygen and substance exchange. Currently, research on fiber scaffold carriers is limited both domestically and internationally. The main fiber scaffolds are electrospun materials, which is primarily used in tissue engineering for in vivo transplantation, and its application in pure large-scale in vitro cell culture is relatively rare. The structural characteristics of electrospun fiber scaffolds are that the electrospun fibers are of nanoscale fine structures with abundant pores, which can simply mimic the distribution of extracellular matrix fibers to facilitate cell attachment and growth. However, the pores are small, and the cells tend to block the relevant pores after attaching in sheets, which severely restricts the exchange of substances. In addition, they tend to float on the surface of the culture medium, which is not conducive to the contact of cells with culture medium.

[0003] The smallest constituent unit of a carrier used for cell culture is a fiber. Said fiber can be, in particular, a polyester fiber.

[0004] Polyester fiber is a product that utilizes polyester in fibrous form. The most commonly used polyester material is polyethylene terephthalate (PET), but other polyesters such as polybutylene terephthalate and polylactic acid are also used. Methods for preparing polyester into fibers can be roughly categorized into melt spinning and solution spinning. The solution spinning is further divided into dry spinning and wet spinning, depending on whether the solvent used is volatile. The melt spinning can be simply divided into direct spinning, mixed spinning, and composite spinning, with composite spinning yielding composite fibers.

[0005] Composite fibers are chemical fibers made from two or more polymers, or the same type of polymer with different properties, through a composite spinning process. Based on the different distributions of the two types of polymers in the cross-section, they can be classified into core-sheath type, segmented-pie type, sea-island type, and side-by-side type.

[0006] However, in the prior art there is a lack of polyester fibers, polyester fiber fabrics, and fiber fabric carriers for efficient cell culture, and there is a lack of methods for obtaining said polyester fibers and for preparing polyester fiber fabrics / fiber fabric carriers to achieve efficient cell culture.Summary of the Invention

[0007] One object of the present invention is to overcome the problem of low cell viability in cell culture when using carriers for cell culture in prior art, and to provide a composite polyester fiber with good fineness uniformity, a polyester fiber fabric made of the composite polyester, a fiber fabric carrier, and preparation methods and uses thereof. The composite polyester fiber of the present invention, as well as the fiber fabric and carrier prepared therefrom, can significantly improve cell viability and accelerate cell proliferation when used for cell culture.

[0008] During research, the inventors found that composite polyester fibers with good fineness uniformity can be obtained by controlling one or more of the average diameter D of the composite polyester fiber, the standard deviation of diameter of the composite polyester fiber, the using amount ratio of the core material to the sheath material, the difference in melting point between the core material and the sheath material, and the difference in intrinsic viscosity between the core material and the sheath material and the standard deviation of the intrinsic viscosity, and by using a high-precision spinneret, preferably ensuring that the using amount ratio of the core material to the sheath material, the average fiber diameter D, and the standard deviation of diameter meet the conditions of the present invention; which fibers can then be used to manufacture polyester fiber fabrics and fiber fabric carriers for cell culture.

[0009] Therefore, a first aspect of the present invention provides a composite polyester fiber having a core-sheath structure; wherein the mass ratio of the core material to the sheath material is x, and 1.5 ≤ x ≤ 4, preferably 2 ≤ x ≤ 3.6, and more preferably 2 ≤ x ≤ 3; and wherein the average diameter D of the composite polyester fiber is 10-40 µm, preferably 17-32 µm, more preferably 18-30 µm, and the standard deviation of diameter is ≤2.2 µm, preferably ≤2.0 µm, more preferably ≤1.8 µm.

[0010] A second aspect of the present invention provides a method for preparing the above-described composite polyester fiber, including: melting a core material and a sheath material respectively to obtain melts, and spinning the melts to obtain the composite polyester fibers.

[0011] A third aspect of the present invention provides a polyester fiber fabric comprising the above-described composite polyester fiber or a composite polyester fiber prepared by the above-described method; for example, the polyester fiber fabric can be prepared by using the composite polyester fiber.

[0012] A fourth aspect of the present invention provides a method for preparing the above-described polyester fiber fabric, including web laying and hot pressing the composite polyester fibers to obtain the polyester fiber fabric.

[0013] A fifth aspect of the present invention provides a fiber fabric carrier for cell culture, wherein the fiber fabric carrier is made of the above-described polyester fiber fabric.

[0014] A sixth aspect of the present invention provides a method for preparing the above-described fiber fabric carrier, including pretreating and / or sterilizing the polyester fiber fabric.

[0015] A seventh aspect of the present invention provides use of the above-described composite polyester fiber for preparing a fiber fabric carrier for cell culture.

[0016] An eighth aspect of the present invention provides use of the above-described polyester fiber fabric or fiber fabric carrier in cell culture.

[0017] A ninth aspect of the present invention provides a method for culturing cells, including sterilizing the above-described polyester fiber fabric and then placing it in a culture medium as a cell carrier, or placing the above-described fiber fabric carrier in a culture medium as a cell carrier, and inoculating cells on the carrier for culturing in vitro, preferably the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0018] Through the above technical solutions, the present disclosure achieves the following beneficial effects: the composite polyester fiber of the present disclosure has a small diameter and a small standard deviation of diameter; when the composite polyester fiber of the present disclosure and the polyester fiber fabric or fiber fabric carrier made therefrom are used for cell culture, they can significantly improve the sugar metabolism capacity of cells; a higher sugar metabolism capacity indicates higher cell proliferation rate and a higher viability (only living cells can metabolize sugar, while dead cells cannot metabolize sugar); that is, the composite polyester fiber and the fiber fabric and carrier manufactured therefrom can achieve better results when used for cell culture; thus, the present disclosure can significantly improve cell viability and accelerate cell proliferation.Detailed description of present invention

[0019] The endpoints of ranges and any values disclosed herein are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of various ranges, the endpoint values of the various ranges and individual point values, as well as individual point values, can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0020] The first aspect of the present invention provides a composite polyester fiber having a core-sheath structure; wherein the mass ratio of the core material to the sheath material is x, and 1.5 ≤ x ≤ 4, preferably 2 ≤ x ≤ 3.6, and more preferably 2 ≤ x ≤ 3; and wherein the average diameter D of the composite polyester fiber is 10-40 µm, preferably 17-32 µm, more preferably 18-30 µm, and the standard deviation of diameter is ≤2.2 µm, preferably ≤2.0 µm, more preferably ≤1.8 µm.

[0021] In the present invention, preferably, the fiber fabric prepared by using the core-sheath structure composite polyester fiber that meets the above-described mass ratio, average diameter and diameter standard deviation can improve the cell proliferation rate and viability, so that the cell sugar metabolism value is at least 15 g / day; however, when the average diameter and diameter standard deviation of the polyester fiber deviate from above ranges, the cell sugar metabolism value is at most 10 g / day.

[0022] In the present invention, the core material is polyethylene terephthalate homopolymer, polyethylene terephthalate copolymer, or a combination thereof, preferably polyethylene terephthalate homopolymer, and the sheath material is polyethylene terephthalate homopolymer, polyethylene terephthalate copolymer, or a combination thereof, preferably polyethylene terephthalate copolymer.

[0023] In the present invention, the amount of the core material used is higher than that of sheath material, and the mass ratio (weight ratio) of the core material to the sheath material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, or a range made by any two of the above values, preferably 2-3.6, more preferably 2-3. Limiting the weight ratio of core material to sheath material within the above preferred ranges can further achieve a smaller diameter standard deviation of the composite polyester fiber.

[0024] In the present invention, the core-sheath mass ratio can be determined by the following method: approximately 10 mg of fiber or fiber fabric sample is dissolved in 50-100 mL of o-chlorophenol at 120-140°C, and then is measured by using liquid chromatography, for example, a high-temperature liquid chromatography, with a nonpolar or weakly polar packing material, such as C18 (ODS); the mobile phase of the liquid chromatography can use a highly polar solvent, such as trifluoroacetic acid, a mixture of phenol and 1,1,2,2-tetrachloroethane (mass ratio 60:40), a mixture of phenol and chloroform (volume ratio 1:1), etc.; two main peaks will appear, namely the sheath material peak and the core material peak, respectively; the area of each peak is obtained by integration and the ratio of the peak areas represents the mass ratio of the core material to the sheath material, i.e., the core-sheath mass ratio. For example, when cyclohexanedimethanol is used as a comonomer in a copolyester, the homopolyester elutes first, and the copolyester elutes later; when isophthalic acid is used as a comonomer in a copolyester, the copolyester elutes first, and the homopolyester elutes later.

[0025] In the present invention, the average diameter of the composite polyester fiber can be 10µm, 15µm, 17µm, 18µm, 20µm, 22µm, 24µm, 26µm, 28µm, 30µm, 32µm, 40µm, or a range made by any two of the above values.

[0026] The standard deviation of diameter of the composite polyester fiber can be 2.2µm, 2.1µm, 2.0µm, 1.9µm, 1.8µm, 1.6µm, 1.4µm, 1.2µm, 1µm, 0.8µm, 0.6µm, 0.4µm, 0.2µm, 0.1µm, or a range made by any two of the above values, such as 0.1-2.2µm.

[0027] In the present invention, the method for measuring the standard deviation of diameter of the composite polyester fiber is as follows: 50 composite polyester fibers are taken randomly, and a small segment is randomly cut therefrom, respectively, for which scanning electron microscope (SEM) image is taken; the diameter of each fiber is measured from the image (one measurement per fiber), with accuracy of 0.1 µm; the arithmetic mean of the diameters of the 50 fibers is the average diameter of the composite polyester fiber; then, the standard deviation of these diameter values is calculated according to standard deviation calculation method. The formula for calculating the standard deviation of diameter is as follows: S d = ∑ i = 1 n d i − d ¯ 2 n − 1 , wherein n is the number of composite polyester fiber samples (i.e., 50), i is the fiber sample serial number, d i is the diameter of the fiber with the serial number i, and d is the arithmetic mean of the diameters of the 50 fiber samples.

[0028] In the present invention, in some embodiments, the composite polyester fiber is a composite polyester fiber with a length of greater than 150 mm.

[0029] According to the present invention, preferably, the intrinsic viscosity of the sheath material is 0.01-0.18 dL / g higher, more preferably 0.01-0.15 dL / g higher than that of the core material, and the standard deviation of intrinsic viscosity of the core material and the sheath material is, respectively, ≤0.02 dL / g, more preferably ≤0.015 dL / g.

[0030] In the present invention, the testing method of the intrinsic viscosity of polyester is the intrinsic viscosity testing method described in GB T 14190-2017. Wherein, the standard deviation of intrinsic viscosity is tested as follows: six samples are randomly taken, and the intrinsic viscosities thereof are measured according to the intrinsic viscosity testing method described in GB T 14190-2017; and the arithmetic mean of the tested intrinsic viscosity values is taken as the intrinsic viscosity of the polyester. A smaller standard deviation of intrinsic viscosity indicates a higher uniformity of the intrinsic viscosity of the same batch of material. The formula for calculating the standard deviation of intrinsic viscosity is as follows: S η = ∑ i = 1 m η i − η ¯ 2 m − 1 , wherein m is the number of samples (i.e., 6), i is the sample serial number, η i is the intrinsic viscosity of the sample with serial number i, and d is the arithmetic mean of the intrinsic viscosities of the 6 samples.

[0031] According to the present invention, the standard deviation of intrinsic viscosity of the core material and the sheath material can each be independently 0.02 dL / g, 0.018 dL / g, 0.015 dL / g, 0.01 dL / g, 0.009 dL / g, 0.008 dL / g, 0.007 dL / g, 0.006 dL / g, 0.005 dL / g, 0.004 dL / g, 0.003 dL / g, 0.002 dL / g, 0.001 dL / g, 0.0001 dL / g, or a range made by any two of the above values, for example, 0.0001-0.02 dL / g.

[0032] According to the present invention, the melting point of the core material is at least 20°C higher than that of the sheath material, for example, 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, or a range made by any two of the above values, such as 20-160°C, higher than that of the sheath material. Preferably, the melting point of the core material is at least 22°C higher, more preferably at least 24°C higher, than that of the sheath material.

[0033] In the present invention, the melting points of the core material and the sheath material are measured by differential scanning calorimetry. Specifically, the sample is heated from 20°C to 300°C at a heating rate of 10°C / min, and the peak temperature of the endothermic peak existing between 100°C and 260°C is taken as the melting point.

[0034] According to the present invention, the inventors further found unexpectedly that the sugar metabolism capacity of cells can be further improved when a fiber fabric prepared from polyester fibers with core material to sheath material mass ratio x and polyester fiber average diameter D satisfying the following formula (1) is used for culturing cells: D / 2 − 3 2 3 D − 9 ≤ x ≤ D / 2 − 1.1 2 1.1 D − 1.21 wherein the numerical value of average diameter D of the composite polyester fiber used in formula (1) is expressed in µm. For example, if the average diameter D is 20 µm, then the numerical value of D in formula (1) is 20.

[0035] Further preferably, the sugar metabolism capacity of cells can be further improved when a composite polyester fiber as wells as a polyester fiber fabric or carrier prepared therefrom is used for culturing cells, wherein the mass ratio of core material to sheath material of the composite polyester fiber is 2-3 and formula (1) is satisfied.

[0036] According to the present invention, in some embodiments, preferably, the core material is polyethylene terephthalate homopolymer. More preferably, the intrinsic viscosity of the polyethylene terephthalate homopolymer is 0.6-0.8 dL / g, such as 0.6 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, or a range made by any two of the above values, preferably 0.62-0.8 dL / g, more preferably 0.65-0.7 dL / g, and the melting point of the polyethylene terephthalate homopolymer is at least 240°C, such as 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, or a range made by any two of the above values, and more preferably 245-280°C.

[0037] According to the present invention, preferably, the standard deviation of intrinsic viscosity of the polyethylene terephthalate homopolymer is ≤0.015 dL / g, more preferably ≤0.01 dL / g, and even more preferably ≤0.005 dL / g.

[0038] According to the present invention, in some embodiments, preferably, the sheath material is polyethylene terephthalate copolymer. More preferably, the intrinsic viscosity of the polyethylene terephthalate copolymer is 0.601-0.9 dL / g, such as 0.61 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86dL / g, 0.88dL / g, 0.9dL / g, or a range made by any two of the above values, preferably 0.61-0.9dL / g, more preferably 0.61-0.82dL / g, and the melting point of the polyethylene terephthalate copolymer is at most 240°C , such as 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, or a range made by any two of the above values, more preferably 130-240°C.

[0039] According to the present invention, preferably, the standard deviation of intrinsic viscosity of the polyethylene terephthalate copolymer is ≤0.015 dL / g, more preferably ≤0.01 dL / g.

[0040] According to the present invention, preferably, the polyethylene terephthalate copolymer comprises structural units derived from terephthalic acid, ethylene glycol, and a comonomer Y, or consists of structural units derived from terephthalic acid, ethylene glycol, and a comonomer Y. The content of structural units derived from the comonomer Y in the polyethylene terephthalate copolymer can be 0.6-20 mol%, such as, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, or a range made by any two of the above values, preferably 0.6-18 mol%, more preferably 0.7-15 mol%. In the present invention, the content of structural units of the comonomer Y refers to the percentage of the molar amount of structural units of the comonomer Y over the total molar amount of structural units of terephthalic acid, ethylene glycol, and the comonomer Y.

[0041] According to the present invention, there is no particular limitation on the type of comonomer Y, as long as the differences in melting point and in intrinsic viscosity between the sheath material and the core material as well as the intrinsic viscosity standard deviations of the sheath material and the core material meet the requirements. Preferably, the comonomer Y can be at least one selected from dicarboxylic acids, diols, and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and further preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol with 3-20 carbon atoms, and further preferably at least one of butanediol, hexanediol, cyclohexanedimethanol and pentanediol (e.g., neopentyl glycol). More preferably, the tetraol is a tetraol with 3-20 carbon atoms. In the present invention, the number of carbon atoms of the diol or the tetraol can each be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, or 20.

[0042] In the present invention, the comonomer Y can also be selected from acid monomers present in other forms, such as acid anhydrides, acyl chlorides, methanol esters, ethanol esters and the like, and alcohol monomers present in other forms, such as ethers, hemiacetals, acetals and the like.

[0043] According to the present invention, preferably, the Sb element content in the core material and sheath material of the polyester fiber is less than 20 ppm. In the present invention, "ppm" refers to the content "by weight".

[0044] The second aspect of the present invention provides a method for preparing the above-described composite polyester fiber, wherein the method including: melting a core material and a sheath material respectively to obtain melts, and spinning the melts to obtain the composite polyester fiber.

[0045] In the present invention, each of the spinneret orifice in the spinneret used in the spinning process has a shape of two concentric circles, wherein the difference between the biggest diameter and smallest diameter of all inner circles of the spinneret orifices (also known as the distribution span, i.e., the diameter of the biggest hole minus the diameter of the smallest hole) is ≤2µm, and the difference between the biggest diameter and smallest diameter of all outer circles of the spinneret orifices (also known as the distribution span, i.e., the diameter of the biggest hole minus the diameter of the smallest hole) is ≤2µm. In the present invention, the difference between the biggest diameter and smallest diameters of all inner circles and the difference between the biggest diameter and smallest diameter of all outer circles can each be independently 0.1µm, 0.5µm, 1.5µm, 2µm, or a range made by any two of the above values, for example, 0.1-2µm.

[0046] In the present invention, it is understood that the core material forms the core structure of the composite polyester fiber after passing through the inner circle of the spinneret orifice, and the sheath material forms the sheath structure of the composite polyester fiber after passing through the outer circle of the spinneret orifice; and then, the composite polyester fiber with core-sheath structure is formed after cooling and stretching.

[0047] According to the present invention, preferably, the inner diameter of the inner circle of the spinneret orifice is 15-25 µm, and the inner diameter of the outer circle of the spinneret orifice is 25-35 µm. In the present invention, the precise orifice diameter of the spinneret is measured by using a profilometer. For example, a Contour GT Profilometer from Bruker can be used.

[0048] According to the present invention, preferably, the process of spinning the melts to obtain composite polyester fibers with core-sheath structure includes the following steps: (1) feeding the melts (the melt of core material and the melt of sheath material) into a spinneret for spinning to obtain filamentous melts with core-sheath structure; (2) cooling the filamentous melts with core-sheath structure to obtain nascent fibers; and (3) stretching the nascent fibers to obtain the composite polyester fibers.

[0049] According to the present invention, preferably, the melt of core material is obtained by melting the core material at 220-280°C (such as, 220°C, 240°C, 260°C, 280°C, or a range made by any two of the above values).

[0050] According to the present invention, preferably, the melt of sheath material is obtained by melting the sheath material at 200-280°C (such as, 200°C, 220°C, 240°C, 260°C, 280°C, or a range made by any two of the above values).

[0051] In the present invention, it is understood that the temperatures of the melt of core material and of the melt of sheath material refer to the temperature of the spinneret when they are extruded from the spinneret outlet.

[0052] According to the present invention, preferably, a side-blowing air is used for cooling, wherein the speed of the side-blowing air can be 1.5-2m / s, for example 1.5m / s, 1.6m / s, 1.7m / s, 1.8m / s, 1.9m / s, 2m / s, or a range made by any two of the above values, more preferably 1.5-1.8m / s; and the temperature thereof is 15-30°C, for example 15°C, 20°C, 25°C, 30°C, or a range made by any two of the above values, more preferably 15-20°C.

[0053] According to the present invention, preferably, the stretching is performed by using a high-speed airflow, wherein the flow velocity of the high-speed airflow is 2500-5500 m / min, for example, preferably 3000-5000 m / min. Preferably, stretching using a high-speed airflow allows the structure of fiber to be further preferentially oriented along the fiber processing direction, thereby in one aspect making the fiber fineness more uniform and in another aspect improving the mechanical properties of the fiber.

[0054] According to the present invention, preferably, the processes used for stretching includes at least one of circular tube stretching process and slit stretching process.

[0055] The circular tube stretching process in the present invention refers to a process in which a number of (e.g., 2-20) nascent fibers are fed into a long circular tube, and the nascent fibers are stretched by airflow at a certain velocity within the circular tube. In the circular tube stretching process, the velocity of airflow can be 2500-5500 m / min, preferably 3000-5000 m / min.

[0056] According to the present invention, preferably, the inner diameter of the circular tube used in the circular tube process is 0.5-5 cm, and the distance between two adjacent circular tubes is 0.5-10 cm. The distance between two adjacent circular tubes refers to the distance between the centers of the two adjacent circular tubes. The length of the circular tube can be 0.1-2 m. The length of the circular tube refers to the distance along the fiber ejection direction.

[0057] According to the present invention, preferably, the spacing distance of slit in the slit stretching process is 0.3-3 cm, and the width thereof is 10-60 cm. It is understood that the width of slit refers to the distance of the slit parallel to the long side of the spinneret. The length of slit can be 10-60 cm. The length of slit refers to the distance along the fiber ejection direction.

[0058] According to the present invention, preferably, the nascent fibers are stretched by a high-speed airflow in the slit, wherein the velocity of the high-speed airflow is 2500-5500 m / min, preferably 3000-5000 m / min.

[0059] The third aspect of the present invention provides a polyester fiber fabric, wherein the polyester fiber fabric comprises the above-described composite polyester fibers or composite polyester fibers prepared by the above-described method. In some embodiments, the polyester fiber fabric is produced by using the above-described composite polyester fibers, for example, the polyester fiber fabric is made of the above-described composite polyester fibers. In some embodiments, the polyester fiber fabric can comprise only the above-described composite polyester fibers, without other fibers. According to the present invention, preferably, the polyester fiber fabric is a nonwoven fabric.

[0060] According to the present invention, preferably, the thickness of the polyester fiber fabric is 0.35-0.6 mm, more preferably 0.4-0.5 mm, and the areal density thereof is 70-160 g / m 2< , more preferably 80-130 g / m 2< .

[0061] In the present invention, the thickness of the polyester fiber fabric can be 0.35mm, 0.4mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.50mm, 0.51mm, 0.52mm, 0.53mm, 0.54mm, 0.55mm, 0.56mm, 0.57mm, 0.58mm, 0.59mm, or 0.60mm, or a range made by any two of the above-described values. The areal density of the polyester fiber fabric can be 70 g / m 2< , 80 g / m 2< , 85 g / m 2< , 90 g / m 2< , 95 g / m 2< , 100 g / m 2< , 105 g / m 2< , 110 g / m 2< , 115 g / m 2< , 120 g / m 2< , 125 g / m 2< , 130 g / m 2< , 135 g / m 2< , 140 g / m 2< , 150 g / m 2< , or 160 g / m 2< , or a range made by any two of the above values.

[0062] Generally, the polyester fiber fabric of the present invention can be prepared from the composite polyester fiber of the present invention by various processes commonly known in the art.

[0063] In the present invention, the standard deviation of diameter of fibers in the polyester fiber fabric is ≤2.4 µm, preferably ≤2.2 µm, and more preferably ≤2.0 µm. The method for measuring the standard deviation of diameter of fibers in the polyester fiber fabric is as follows: a scanning electron microscope (SEM) image of polyester fiber fabric is taken, and the diameters of 50 fibers randomly selected from the image are measured (one measurement per fiber), with accuracy of 0.1 µm; then, the standard deviation of these diameter values is calculated according to the standard deviation calculation method. The formula for calculating the standard deviation of diameter is as follows: S d = ∑ i = 1 n d i − d ¯ 2 n − 1 , wherein n is the number of composite polyester fiber samples (i.e., 50), i is the fiber sample serial number, d i is the diameter of the fiber with the serial number i, and d is the arithmetic mean of the diameters of the 50 fiber samples.

[0064] The standard deviation of diameter of fibers in the polyester fiber fabric can be 2.4µm, 2.3µm, 2.2µm, 2.1µm, 2.0µm, 1.9µm, 1.8µm, 1.6µm, 1.4µm, 1.2µm, 1µm, 0.8µm, 0.6µm, 0.4µm, 0.2µm, 0.1µm, or a range made by any two of the above values, such as 0.1-2.4µm.

[0065] The method for testing the areal density of the fiber fabric in the present invention is as follows: a fiber fabric with an area of 1 cm 2< is weighed by using a balance with a readability of 0.1 mg, and then, the mass of the fiber fabric is divided by its area. In the present invention, the thickness of the fiber fabric is measured by using a thickness gauge, for example the thickness can be measured by a dial-type thickness gauge. For example, a Mitutoyo 547-313 thickness gauge from Mitutoyo, Japan can be used.

[0066] The fourth aspect of the present invention provides a method for preparing the above-described polyester fiber fabric, comprising web laying and hot pressing the composite polyester fiber of the present invention to obtain the polyester fiber fabric.

[0067] According to the present invention, preferably, the temperature for hot pressing is 180-220°C.

[0068] Instead of using long fibers, the method can also include, successively, wet laying a slurry containing short fibers of the composite polyester fibers and heat treating.

[0069] In the method, the length of the short polyester fibers can be 1 mm, 2 mm, 3 mm, 4 mm, 5 mm, 6 mm, 7 mm or 8 mm, or a range made by any two of the above values.

[0070] Preferably, the length of the short polyester fibers is 1-4 mm, more preferably 2-3 mm.

[0071] Preferably, the content of polyester fiber in the slurry containing the polyester fiber is 5-30 wt%, such as, 5 wt%, 8 wt%, 10 wt%, 12 wt%, 14 wt%, 16 wt%, 18 wt%, 20 wt%, 25 wt%, 30 wt%, or a range made by any two of the above values, more preferably 10-20 wt%.

[0072] According to the present invention, preferably, the slurry containing the polyester fiber further comprise a dispersant, wherein the dispersant is at least one selected from polyethylene glycols, fatty alcohols and polyacrylamides.

[0073] According to the present invention, preferably, the content of dispersant in the slurry containing the polyester fiber is 0.05-2 wt%; such as, 0.05 wt%, 0.5 wt%, 0.8 wt%, 0.9 wt%, 1 wt%, 1.1 wt%, 1.2 wt%, 1.3 wt%, 1.4 wt%, 1.5 wt%, 1.8 wt%, 2 wt%, or a range made by any two of the above values.

[0074] According to the present invention, preferably, the dispersant is a combination of a polyethylene glycol, a fatty alcohol and a polyacrylamide. More preferably, the weight ratio of the polyethylene glycol, the fatty alcohol and the polyacrylamide is 1:0.5-2:0.5-2, and even more preferably 1:1-1.2:1-1.2.

[0075] In the present invention, the weight ratio of the polyethylene glycol to the fatty alcohol can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, or a range made by any two of the above values.

[0076] In the present invention, the weight ratio of the polyethylene glycol to the polyacrylamide can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, or a range made by any two of the above values.

[0077] According to the present invention, preferably, the polyethylene glycol has a molecular weight of 400-4000 g / mol (such as, 400 g / mol, 500 g / mol, 600 g / mol, 700 g / mol, 800 g / mol, 900 g / mol, 1000 g / mol, 1500 g / mol, 1600 g / mol, 1700 g / mol, 2000 g / mol, or a range made by any two of the above values), more preferably 500-2000 g / mol.

[0078] According to the present invention, preferably, the fatty alcohol is a fatty alcohol with 3-12 carbon atoms; more preferably, the fatty alcohol has 1-3 hydroxyl groups; even more preferably, the fatty alcohol is at least one selected from octanol, heptanol, hexanol, pentanol, and butanol.

[0079] According to the present invention, preferably, the polyacrylamide has a molecular weight of 50000-5000000 g / mol (such as, 50000 g / mol, 100000 g / mol, 150000 g / mol, 180000 g / mol, 200000 g / mol, 220000 g / mol, 240000 g / mol, 250000 g / mol, 300000 g / mol, 400000 g / mol, 1000000 g / mol, 5000000 g / mol, or a range made by any two of the above values), more preferably 200000-250000 g / mol.

[0080] In the present invention, all molecular weights are number average molecular weights, unless otherwise specified.

[0081] According to the present invention, preferably, the temperature for the heat treating is 180-220°C.

[0082] The fifth aspect of the present invention provides a fiber fabric carrier for cell culture, wherein the fiber fabric carrier is made of the above-described polyester fiber fabric. In some embodiments, preferably, the fiber fabric carrier is a sheet-like fiber fabric carrier.

[0083] In the present invention, the polyester fiber fabric of the present invention can be pretreated and / or sterilized to prepare the fiber fabric carrier.

[0084] Preferably, the pretreatment includes soaking the polyester fiber fabric in a hydrogen peroxide solution.

[0085] Preferably, the sterilization includes at least one of ethylene oxide sterilization, high-temperature steam sterilization, and irradiation sterilization, more preferably high-temperature steam sterilization.

[0086] According to the present invention, preferably, the conditions for high-temperature steam sterilization include: a temperature of 115-135°C, such as 115°C, 120°C, 125°C, 130°C, 135°C, or a range made by any two of the above values, and a time of 10-60 min, such as 10 min, 20 min, 30 min, 40 min, 50 min, 60 min, or a range made by any two of the above values.

[0087] According to the present invention, preferably, the conditions for irradiation sterilization include: irradiating the pretreated fiber fabric with high-energy electron beams, the irradiation dose being 5-30 kGy, and the irradiation time being not more than 10 seconds.

[0088] According to the present invention, preferably, the fiber fabric is pretreated before sterilization. In some embodiments, the pretreatment can be performed by pretreating the fiber fabric in a hydrogen peroxide solution.

[0089] According to the present invention, preferably, the concentration of H 2 O 2 in the hydrogen peroxide solution is 5-10 wt%.

[0090] According to the present invention, preferably, the conditions for pretreatment include: a temperature of 15-40°C and a time of 0.5-4 hours.

[0091] According to the present invention, preferably, the pretreated fiber fabric can be washed under ultrasonic treatment and then sterilized.

[0092] According to the present invention, preferably, the washing agent used for washing is water, preferably double-distilled water and / or ultrapure water.

[0093] According to the present invention, preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0094] In some embodiments, optionally, the fiber fabric carrier can include multiple, preferably 2-7, layers of the polyester fiber fabric of the present invention, or can be made of multiple, preferably 2-7, layers of the polyester fiber fabric of the present invention. Preferably, there are weld lines, such as, 2-5 weld lines, between the multiple layers of polyester fiber fabric. Preferably, the shortest distance between any two weld lines is >0.5 mm. More preferably, the weld lines are straight lines. In the present invention, the weld line refers to the line formed by the welding path, and the welding refers to the partial fusion of the multiple layers of polyester fiber fabric by using heat.

[0095] In some embodiments, the fiber fabric carrier is preferably circular, elliptical, or rectangular. Preferably, the diameter of the circle, the major axis of the ellipse, or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm.

[0096] The sixth aspect of the present invention provides a method for preparing the above-described fiber fabric carrier, wherein the method includes: pretreating and / or sterilizing the polyester fiber fabric.

[0097] Preferably, the pretreatment includes soaking the polyester fiber fabric in a hydrogen peroxide solution.

[0098] Preferably, the sterilization includes at least one of ethylene oxide sterilization, high-temperature steam sterilization, and irradiation sterilization, more preferably high-temperature steam sterilization.

[0099] The seventh aspect of the present invention provides use of the composite polyester fiber of the present invention or the composite polyester fiber prepared by the method of the present invention for preparing a composite polyester fiber for preparing a fiber fabric carrier for cell culture.

[0100] The eighth aspect of the present invention provides an use of the polyester fiber fabric or fiber fabric carrier of the present invention for cell culture.

[0101] According to the present invention, preferably, the use can increase the sugar metabolism rate of cells. Preferably, the sugar metabolism rate of cells can be increased to at least 15 g / day, such as 15-35 g / day, more preferably 20-33 g / day, and even more preferably 27-32 g / day, by using the fiber fabric of the present invention for culturing cells.

[0102] Preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0103] The ninth aspect of the present invention provides a method for culturing cells, the method including: sterilizing the polyester fiber fabric of the present invention and then placing it in a culture medium as a carrier, or placing the fiber fabric carrier of the present invention in a culture medium as a carrier, and inoculating cells on the carrier and culturing in vitro, preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0104] The present invention will be described in detail below through examples. In the following examples, the spinneret was obtained from Changzhou Jier Precision Machinery Manufacturing Co., Ltd, unless otherwise specified.

[0105] The Sb element contents in both the core material and the sheath material were less than 20 ppm.

[0106] The room temperature was approximately "15-20°C".

[0107] In the present invention, the measuring method of melting point was as follows: placing approximately 5 mg of sample in a differential scanning calorimeter (DSC) (Q100 from TA Instruments), heating the sample from 20°C to 300°C at a heating rate of 10°C / min, and observing the endothermic peak present between 100°C and 260°C. The sample exhibited an endothermic single peak within this temperature range. The temperature of the peak was taken as the melting point.

[0108] In the following examples, the preparing method of the self-made polyesters involved was as follows: mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and optional comonomer Y (e.g., 1,4-cyclohexanedimethanol or isophthalic acid, analytical grade) in a reaction vessel according to the amounts required by the final product, heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, evacuating by using an oil pump, and reacting for about 2 hours to obtain the required polyester.

[0109] Specifically, the preparing method of the self-made polyester A was as follows: adding 166g of PTA and 64g of EG to a reaction vessel, stirring and heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, evacuating by using an oil pump, and reacting for about 2 hours to obtain the required polyester. The preparing method of the self-made polyester B was as follows: adding 133g of PTA and 51g of EG to a reaction vessel, stirring and heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, evacuating by using an oil pump, and reacting for about 2 hours to obtain the required polyester. The preparing method of the self-made polyester C was as follows: adding 133g of PTA, 35.4g of EG, and 34.6g of 1,4-cyclohexanedimethanol to a reaction vessel, stirring and heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, evacuating by using an oil pump, and reacting for about 2 hours to obtain the required polyester.Example 1(1) Preparation of polyester fibers:

[0110] Polyethylene terephthalate homopolymer (manufactured by Sinopec Yizheng Chemical Fibre Co., Ltd., hereinafter referred to as "Yizheng Chemical Fibre", type FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261°C) was used as the core material. Polyethylene terephthalate copolymer (Yizheng Chemical Fiber, type FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer cyclohexanedimethanol, comonomer content 15 mol%, melting point 140°C) was used as the sheath material. The mass ratio of core material to sheath material was 3, and the materials were extruded out from the spinneret at an outlet temperature of approximately 280°C. Wherein, the spinneret had 48 spinneret orifices, each of the spinneret orifice had a shape of two concentric circles, wherein the inner circle had an inner diameter of 20 micrometers and the distribution span (the diameter of the biggest hole minus the diameter of the smallest hole) was 1.2 micrometers; the outer circle had an inner diameter of 30 micrometers and the distribution span was 1.1 micrometers. After the polymer melts were extruded out from the spinneret, they were cooled by blowing air at room temperature at 1.5-1.8 m / s; and the cooled fibers were fed averagely or nearly averagely into five circular tubes made of steel (the circular tubes had an inner diameter of 2 cm and a distance of 3.5 cm between two adjacent circular tubes, and the circular tubes had a length of 1 m); in the circular tubes, the cooled fibers were stretched at an air velocity of approximately 4000-4500 m / min to obtain composite polyester fibers.

[0111] The average diameter of the above-described composite polyester fibers was measured and the standard deviation of diameter was calculated. The testing results are shown in Table 1.

[0112] The method for measuring the average diameter of the composite polyester fiber is as follows: 50 composite polyester fibers are taken randomly, and a small segment is randomly cut therefrom, respectively, for which scanning electron microscope (SEM) image (S-4800, Hitachi, Ltd.) is taken; the diameter of each fiber is measured from the image, with accuracy of 0.1 µm; the mean of the diameters is calculated according to the mean value calculation method; and, the standard deviation of these diameter values is calculated according to standard deviation calculation method. The testing results are shown in Table 1.(2) Preparation of polyester fiber fabric:

[0113] The obtained polyester fibers were laid into a web by using a web laying machine; then, the laid web of the fibers with a certain thickness was hot pressed at 190-200°C using a polished, non-embossed hot roller to obtain the fiber fabric.

[0114] The method for testing the areal density of the fiber fabric was as follows: a fiber fabric with an area of 1 cm 2< was weighed by using a balance with a readability of 0.1 mg, and then, the mass of the fiber fabric was divided by its area. The thickness of the fiber fabric was measured as follows: thickness was measured by using Mitutoyo thickness gauge from Mitutoyo, Japan (model 547-313). The areal density and thickness of the fiber fabric are shown in Table 1.Example 2

[0115] The method of Example 1 was repeated, except that polyethylene terephthalate copolymer (Yizheng Chemical Fiber, type BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol%, melting point 237°C) was used as the sheath material.Example 3

[0116] The method of Example 1 was repeated, except that the mass ratio of the core material to the sheath material was 2, and for each of the spinneret orifice of the spinneret used, the inner diameter of the inner circle was 18 micrometers with a distribution span of 1.1 micrometers, and the inner diameter of the outer circle was 32 micrometers with a distribution span of 1.6 micrometers.Example 4

[0117] The method of Example 1 was repeated, except that the mass ratio of the core material to the sheath material was 3.6.Example 5

[0118] The method of Example 1 was repeated, except that the mass ratio of the core material to the sheath material was 1.6.Example 6

[0119] The method of Example 1 was repeated, except that the cooled fibers were fed into a slit with a spacing distance of 1 cm, a slit width of 50 cm, and a length of 50 cm and the cooled fibers were stretched with an air speed of 3500-4000 m / min to obtain polyester fibers.Example 7

[0120] The method of Example 6 was repeated, except that polyethylene terephthalate copolymer (Yizheng Chemical Fiber, type BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol% based on all monomers, melting point 237°C) was used as the sheath material.Example 8

[0121] The method of Example 6 was repeated, except that the mass ratio of the core material to the sheath material was 2, and for the spinneret used, the inner diameter of the inner circle of the spinneret orifice was 18 micrometers with a distribution span of 1.1 micrometers, and the inner diameter of the outer circle of the spinneret orifice was 32 micrometers with a distribution span of 1.6 micrometers.Example 9

[0122] The method of Example 6 was repeated, except that the mass ratio of the core material to the sheath material was 3.6.Example 10

[0123] The method of Example 6 was repeated, except that the mass ratio of the core material to the sheath material was 1.6.Example 11(1) Preparation of polyester fibers:

[0124] Polyester fibers were obtained according to step (1) in Example 1.(2) Preparation of polyester fiber fabric:

[0125] The polyester fibers obtained in step (1) were cut to a cut length of 3 ± 0.1 mm to obtain polyester short fibers. The polyester short fibers were mixed evenly with water and a dispersant to obtain a slurry containing polyester short fibers; wherein the slurry containing polyester short fibers had a content of polyester short fibers of 15 wt% and a content of dispersant of 1 wt%, and wherein the dispersant was polyethylene glycol, fatty alcohol and polyacrylamide in a weight ratio of 1:1:1; wherein the molecular weight of the polyethylene glycol was 800 g / mol, the fatty alcohol was n-octanol, and the molecular weight of the polyacrylamide was 200000 g / mol. The slurry containing polyester short fibers was then conveyed to the web forming zone of a wet laying machine, where water was filtered out through a screen, and the polyester short fibers formed a fiber web on the web forming screen. After the fiber web was dried in air, it was hot-pressed at 190-200°C by using a polished, non-embossed hot roller to obtain a fiber fabric.Example 12

[0126] The method of Example 11 was repeated, except that polyethylene terephthalate copolymer (Yizheng Chemical Fiber, type BG804, intrinsic viscosity 0.801dL / g, standard deviation of intrinsic viscosity 0.005dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75mol% based on all monomers, melting point 237°C) was used as the sheath material in step (1).Example 13

[0127] The method of Example 11 was repeated, except that in step (1), the mass ratio of the core material to the sheath material was 2, and for the spinneret used, the inner diameter of the inner circle of the spinneret orifice was 18 micrometers with a distribution span of 1.1 micrometers, and the inner diameter of the outer circle of the spinneret orifice was 32 micrometers with a distribution span of 1.6 micrometers.Example 14

[0128] The method of Example 11 was repeated, except that the mass ratio of the core material to the sheath material was 3.6 in step (1).Example 15

[0129] The method of Example 11 was repeated, except that the mass ratio of the core material to the sheath material was 1.6 in step (1).Example 16

[0130] The method of Example 11 was repeated, except that the polyester fibers obtained in step (1) were cut to a cut length of 2 ± 0.1 mm to obtain polyester short fibers in step (2). The polyester short fibers were mixed evenly with water and a dispersant to obtain a slurry containing polyester short fibers; wherein the slurry containing polyester short fibers had a content of polyester short fibers of 18 wt% and a content of dispersant of 1.5 wt%, and wherein the dispersant was polyethylene glycol, fatty alcohol and polyacrylamide in a weight ratio of 1:1.2:1.2; wherein the molecular weight of the polyethylene glycol was 1600 g / mol, the fatty alcohol was n-octanol, and the molecular weight of the polyacrylamide was 250000 g / mol.Example 17

[0131] The method of Example 11 was repeated, except that the fatty alcohol and the polyacrylamide were replaced with polyethylene glycol in equal weight in step (2).Comparative Example 1

[0132] The method of Example 1 was repeated, except that the mass ratio of the core material to the sheath material was 1.Comparative Example 2

[0133] The method of Example 1 was repeated, except that the mass ratio of the core material to the sheath material was 5.Comparative Example 3

[0134] The method of Example 1 was repeated, except that the raw material used for the polyethylene terephthalate homopolymer was the self-made polyester A with an intrinsic viscosity of 0.51 dL / g, a standard deviation of intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.Comparative Example 4

[0135] The method of Example 1 was repeated, except that the spinneret orifices of the spinneret used did not have the structure of concentric circles, but rather a parallel structure with the circle being divided into two parts. Wherein the inner diameter of the circular spinneret orifices was 30 micrometers with a distribution span of 1.1 micrometers.Comparative Example 5

[0136] The method of Example 1 was repeated, except that the spinneret used had a larger distribution span of hole diameter, wherein the inner diameter of the inner circles was 20 micrometers with a distribution span of 3.6 micrometers and the inner diameter of the outer circles was 30 micrometers with a distribution span of 5.3 micrometers.Comparative Example 6

[0137] The method of Example 1 was repeated, except that polyethylene terephthalate homopolymer was replaced with BG85 from Yizheng Chemical Fiber, with intrinsic viscosity of 0.879 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 248°C.Comparative Example 7

[0138] The method of Example 1 was repeated, except that the polyethylene terephthalate homopolymer was replaced with the self-made polyester B with intrinsic viscosity of 0.675 dL / g, standard deviation of intrinsic viscosity of 0.031 dL / g, and melting point of 260°C; the polyethylene terephthalate copolymer was replaced with the self-made polyester C with intrinsic viscosity of 0.782 dL / g, standard deviation of intrinsic viscosity of 0.025 dL / g, and melting point of 143°C, comonomer being cyclohexanedimethanol, and the comonomer content being 15 mol%.Comparative Example 8

[0139] The method of Example 1 was repeated, except that polyethylene terephthalate homopolymer was replaced with BG804 from Yizheng Chemical Fiber, with intrinsic viscosity of 0.801 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 237°C.Comparative Example 9

[0140] The method of Example 1 was repeated, except that the polyethylene terephthalate copolymer was replaced with BG85 from Yizheng Chemical Fiber with intrinsic viscosity of 0.879 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 248°C.Comparative Example 10

[0141] The method of Example 1 was repeated, except that the air speed in the circular tubes was 1500-2000 m / min.Comparative Example 11

[0142] The method of Example 1 was repeated, except that the melt filaments, after cooling, were not fed into the circular tubes, but were stretched and laid into a web using the oscillating layingprocess.Comparative Examples 12-13

[0143] The method of Example 1 was repeated, except that different areal densities and thicknesses of fiber fabrics were obtained by adjusting the process parameters of web laying and hot pressing; wherein the areal densities and thicknesses of the fiber fabrics are shown in Table 1.Comparative Example 14

[0144] The method of Example 6 was repeated, except that the mass ratio of the core material to the sheath material was 1.Comparative Example 15

[0145] The method of Example 6 was repeated, except that the mass ratio of the core material to the sheath material was 5.Comparative Example 16

[0146] The method of Example 6 was repeated, except that the raw material used for the polyethylene terephthalate homopolymer was the self-made polyester A with intrinsic viscosity of 0.51 dL / g, standard deviation of intrinsic viscosity of 0.01 dL / g, and melting point of 260°C.Comparative Example 17

[0147] The method of Example 6 was repeated, except that the spinneret orifices of the spinneret used did not have the structure of concentric circles, but rather a parallel structure with the circle being divided into two parts. Wherein the inner diameter of the circular spinneret orifices was 30 micrometers with a distribution span of 1.1 micrometers.Comparative Example 18

[0148] The method of Example 6 was repeated, except that the spinneret used had a larger distribution span of hole diameter, wherein the inner diameter of the inner circles was 20 micrometers with a distribution span of 3.6 micrometers and the inner diameter of the outer circles was 30 micrometers with a distribution span of 5.3 micrometers.Comparative Example 19

[0149] The method of Example 6 was repeated, except that the raw material used for polyethylene terephthalate homopolymer was BG85 from Yizheng Chemical Fiber with intrinsic viscosity of 0.879 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 248°C.Comparative Example 20

[0150] The method of Example 6 was repeated, except that the polyethylene terephthalate homopolymer was replaced with the self-made polyester B with intrinsic viscosity of 0.675 dL / g, standard deviation of intrinsic viscosity of 0.031 dL / g, and melting point of 260°C; the polyethylene terephthalate copolymer was replaced with the self-made polyester C with intrinsic viscosity of 0.782 dL / g, standard deviation of intrinsic viscosity of 0.025 dL / g, and melting point of 143°C, comonomer being cyclohexanedimethanol, and comonomer content being 15 mol%.Comparative Example 21

[0151] The method of Example 6 was repeated, except that polyethylene terephthalate homopolymer was replaced with BG804 from Yizheng Chemical Fiber with intrinsic viscosity of 0.801 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 237°C.Comparative Example 22

[0152] The method of Example 6 was repeated, except that the polyethylene terephthalate copolymer was replaced with BG85 from Yizheng Chemical Fiber with intrinsic viscosity of 0.879 dL / g, standard deviation of intrinsic viscosity of 0.005 dL / g, and melting point of 248°C.Comparative Example 23

[0153] The method of Example 6 was repeated, except that the air speed in the slit was 1500-2000 m / min.Comparative Example 24

[0154] The method of Example 11 was repeated, except that the mass ratio of the core material to the sheath material was 1.Comparative Example 25

[0155] The method of Example 11 was repeated, except that polyethylene terephthalate homopolymer was replaced with the self-made polyester A with intrinsic viscosity of 0.51 dL / g, standard deviation of intrinsic viscosity of 0.01 dL / g, and melting point of 260°C.Comparative Example 26

[0156] The method of Example 11 was repeated, except that the spinneret used had a larger distribution span of hole diameter, wherein the inner diameter of the inner circles was 20 micrometers with a distribution span of 3.6 micrometers and the inner diameter of the outer circles was 30 micrometers with a distribution span of 5.3 micrometers.Comparative Example 27

[0157] The method of Example 11 was repeated, except that the polyester fibers obtained in step (1) were cut to a cut length of 10 ± 0.1 mm to obtain polyester short fibers in step (2).Comparative Example 28

[0158] The method of Example 1 was repeated, except that the speed of the receiving screen of the web laying machine was increased to three times of the original speed during web laying.Test examples

[0159] Pretreatment and sterilization of the fiber fabrics and sheet-like fiber fabric carriers: they were soaked in a 7 wt% hydrogen peroxide aqueous solution at room temperature for a time of 60 min; then they were ultrasonically washed with ultrapure water, and were dried at 80°C. The dried fiber fabrics and sheet-like fiber fabric carriers were sterilized by high-temperature steam, wherein the temperature of high-temperature steam sterilization was 121°C and the time was 60 min.

[0160] Then, the sugar metabolism capacity of Vero cells was tested when sterilized fiber fabrics or sheet-like fiber fabric carriers were used as carriers. The sugar metabolism test method was as follows: the fiber fabrics or sheet-like fiber fabric carriers were cut into 6mm × 6mm pieces; 100g of 6mm × 6mm fiber fabrics or sheet-like fiber fabric carriers, 8L of PBS buffer, and approximately 200mL of 199 culture medium (purchased from Beijing Qingda Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor; and 10% of premium newborn calf serum (purchased from Lanzhou Minhai Bioengineering Co., Ltd.) relative to the culture medium was added, and an appropriate amount of glucose solution with a concentration of 20 wt% was added so that the total glucose concentration was 0.5 wt%. Vero cells (purchased from National Biomedical Experimental Cell Resource Bank, fourth-generation Vero cells, inoculating amount 0.5 × 10 6< cells per 1cm 2< fiber fabric) were inoculated and cultured at 37°C with aeration; during culture, the pH was set to be 7.3, DO (percentage of dissolved oxygen relate to saturated dissolved oxygen) to be 60%, and carbon dioxide concentration to be 5%; and it was stirred slowly and was cultured for 7 days. Samples were taken every 24 hours, and glucose content was measured by using a glucose assay kit (purchased from Nanjing Jiancheng Bioengineering Institute), and the glucose concentration was replenished to 0.5 wt% by using the glucose solution with a concentration of 20 wt%. The decrease in glucose content measured was converted into glucose consumption rate value, expressed in g / day. The glucose consumption rate was plotted against the culture time, and the value at 100 hours was taken as the 100-hour sugar metabolism rate value. The 100-hour sugar metabolism rate values are shown in Table 1. Table 1Average diameter of composite polyester fibers (µm)Standard deviation of diameter of composite polyester fibers (µm)Areal density of fiber fabrics (g / m 2< )Thickness of fiber fabrics (mm)Standard deviation of diameter of fibers in fiber fabrics (µm)100-hour sugar metabolis m rate (g / day)Satisfying formula (1)Ex. 1221.41180.421.530yesEx. 2181.51180.421.728yesEx. 3291.61200.441.728yesEx. 4181.41180.411.619noEx. 5291.81190.431.921noEx. 6211.91170.422.026yesEx. 7181.81180.431.923yesEx. 8282.21160.432.325yesEx. 9182.11190.442.216noEx. 10292.11180.432.217noEx. 11221.41150.431.629yesEx. 12181.51140.431.627yesEx. 13291.61160.441.627yesEx. 14181.41150.441.522noEx. 15291.81130.422.021noEx. 16221.41150.421.627yesEx. 17221.41130.411.716yesComp. Ex. 1143.51160.424.15yesComp. Ex. 2282.61190.432.93yesComp. Ex. 3274.11190.444.48yesComp. Ex. 4224.61160.414.91yesComp. Ex. 5226.11170.416.85yesComp. Ex. 6263.21190.443.42yesComp. Ex. 7205.11180.425.43yesComp. Ex. 8203.11190.443.28yesComp. Ex. 9252.41180.422.76yesComp. Ex. 10282.41170.422.89yesComp. Ex. 11386.81180.427.31yesComp. Ex. 12221.42360.841.69yesComp. Ex. 13221.42230.311.62yesComp. Ex. 14153.91170.414.33yesComp. Ex. 15294.21200.454.51yesComp. Ex. 16285.21180.435.71yesComp. Ex. 17234.81170.425.23yesComp. Ex. 18226.51160.406.92yesComp. Ex. 19254.11180.424.46yesComp. Ex. 20214.91170.425.12yesComp. Ex. 21203.51190.443.84yesComp. Ex. 22272.91170.413.13yesComp. Ex. 23282.81160.413.25yesComp. Ex. 24143.51150.423.73yesComp. Ex. 25274.11160.434.22yesComp. Ex. 26226.11150.436.63yesComp. Ex. 27221.41160.431.65yesComp. Ex. 28221.4400.151.68yes

[0161] As can be seen from the results in Table 1, the composite polyester fibers of the present invention or the composite polyester fibers prepared by the method of the present invention have a relatively small standard deviation of diameter. The carriers prepared from the composite polyester fibers of the present invention can significantly improve the sugar metabolism capacity of cells.

[0162] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the contents disclosed in the present invention and are all within the protection scopes of the present invention.

Claims

1. A composite polyester fiber, characterized in that the composite polyester fiber has a core-sheath structure; wherein the mass ratio of the core material to the sheath material is x, and 1.5 ≤ x ≤ 4, preferably 2 ≤ x ≤ 3.6, and more preferably 2 ≤ x ≤ 3; and wherein the average diameter D of the composite polyester fiber is 10-40 µm, preferably 17-32 µm, more preferably 18-30 µm, and the standard deviation of diameter is ≤2.2 µm, preferably ≤2.0 µm, more preferably ≤1.8 µm.

2. The composite polyester fiber according to claim 1, wherein the melting point of the core material is at least 20°C higher, preferably at least 22°C higher than that of the sheath material; wherein the intrinsic viscosity of the sheath material is 0.01-0.18 dL / g higher, preferably 0.01-0.15 dL / g higher than that of the core material, and wherein the standard deviation of intrinsic viscosity of the core material and the sheath material, respectively, is ≤0.02 dL / g, preferably ≤0.015 dL / g; and / or wherein the core material is polyethylene terephthalate homopolymer, polyethylene terephthalate copolymer, or a combination thereof, preferably polyethylene terephthalate homopolymer, and wherein the sheath material is polyethylene terephthalate homopolymer, polyethylene terephthalate copolymer, or a combination thereof, preferably polyethylene terephthalate copolymer.

3. The composite polyester fiber according to any one of claims 1 to 2, characterized in that the mass ratio x of the core material to the sheath material of the composite polyester fiber and the average diameter D of the composite polyester fiber satisfy the following formula (1): D / 2 − 3 2 3 D − 9 ≤ x ≤ D / 2 − 1.1 2 1.1 D − 1.21 wherein the numerical value of average diameter D of the composite polyester fiber used in formula (1) is expressed in micrometers.

4. The composite polyester fiber according to any one of claims 1 to 3, wherein the core material is polyethylene terephthalate homopolymer; preferably, the intrinsic viscosity of the polyethylene terephthalate homopolymer is 0.6-0.8 dL / g, more preferably 0.62-0.8 dL / g, more preferably 0.65-0.7 dL / g, and the melting point is at least 240°C, more preferably 245-280°C, and / or preferably, the standard deviation of intrinsic viscosity of the polyethylene terephthalate homopolymer is ≤0.015 dL / g, more preferably ≤0.01 dL / g, more preferably ≤0.005 dL / g.

5. The composite polyester fiber according to any one of claims 1 to 4, wherein the sheath material is polyethylene terephthalate copolymer; preferably, the intrinsic viscosity of the polyethylene terephthalate copolymer is 0.601-0.9 dL / g, more preferably 0.61-0.9 dL / g, and even more preferably 0.61-0.82 dL / g, and the melting point is not higher than 240°C, more preferably 130-240°C, and / or preferably, the standard deviation of intrinsic viscosity of the polyethylene terephthalate copolymer is ≤0.015 dL / g, and more preferably ≤0.01 dL / g.

6. The composite polyester fiber according to claim 5, wherein the polyethylene terephthalate copolymer comprises structural units derived from terephthalic acid, ethylene glycol, and a comonomer Y, and the content of structural units derived from the comonomer Y in the polyethylene terephthalate copolymer is 0.6-20 mol%, preferably 0.6-18 mol%, more preferably 0.7-15 mol%; preferably, the comonomer Y is at least one selected from dicarboxylic acids, diols, and tetraols, and more preferably at least one selected from isophthalic acid, phthalic acid, butanediol, hexanediol, cyclohexanedimethanol, and pentanediol.

7. A method for preparing composite polyester fibers according to any one of claims 1 to 6, characterized in that the method includes: melting a core material and a sheath material respectively to obtain melts, and spinning the melts to obtain the composite polyester fibers.

8. The method for preparing composite polyester fibers according to claim 7, wherein, each of the spinneret orifice in the spinneret used in the spinning process has a shape of two concentric circles, the difference between the biggest diameter and smallest diameter of all inner circles of the spinneret orifices is ≤2µm, and the difference between the biggest diameter and smallest diameter of all outer circles of the spinneret orifices is ≤2µm, and / or wherein the inner diameter of inner circles of the spinneret orifices is 15-25 µm, and the inner diameter of outer circles of the spinneret orifices is 25-35 µm.

9. The method according to any one of claims 7 to 8, wherein the method includes: (1) feeding the molten core material and sheath material into a spinneret to conduct spinning to obtain filamentous melts with core-sheath structure; (2) cooling the filamentous melts with core-sheath structure to obtain nascent fibers; and (3) stretching the nascent fibers to obtain the composite polyester fibers.

10. The method according to claim 9, wherein the nascent fibers are stretched by using a high-speed airflow, preferably a high-speed airflow of 2500-5500 m / min; preferably, the stretching includes at least one of the circular tube stretching process and the slit stretching process.

11. The method according to claim 10, wherein in the slit stretching process, the slit has a spacing distance of 0.3-3 cm, a width of 10-60 cm, and a length of 10-60 cm; or in the circular tube process, the circular tube has an inner diameter of 0.5-5cm, a distance between two adjacent circular tubes of 0.5-10cm, and a circular tube length of 0.1-2m.

12. A polyester fiber fabric, characterized in that the polyester fiber fabric comprises composite polyester fibers according to any one of claims 1 to 6 or composite polyester fibers prepared by the method according to any one of claims 7 to 11.

13. The polyester fiber fabric according to claim 12, wherein the thickness of the polyester fiber fabric is 0.35-0.6 mm, preferably 0.4-0.5 mm, and the areal density of the polyester fiber fabric is 70-160 g / m2, preferably 80-130 g / m2; and / or the polyester fiber fabric is a nonwoven fabric; and / or the standard deviation of diameter of fibers in the polyester fiber fabric is ≤2.4µm, preferably ≤2.2µm, and more preferably ≤2.0µm.

14. A method for preparing the polyester fiber fabric according to any one of claims 12-13, including web laying the composite polyester fibers and hot pressing to obtain the polyester fiber fabric.

15. The method according to claim 14, wherein the temperature for hot pressing is 180-220°C.

16. The method according to claim 14 or 15, wherein a slurry containing short fibers of the composite polyester fibers is wet-laid into a web.

17. The method according to claim 16, wherein the content of composite polyester fibers in the slurry is 5-30 wt%, preferably 10-20 wt%, and / or the slurry further comprises a dispersant, preferably the dispersant is at least one selected from polyethylene glycols, fatty alcohols and polyacrylamides, and / or preferably, the content of the dispersant in the slurry is 0.05-2 wt%.

18. The method according to claim 17, wherein the dispersant is a combination of a polyethylene glycol, a fatty alcohol and a polyacrylamide, wherein the weight ratio of the polyethylene glycol, fatty alcohol and polyacrylamide is 1:0.5-2:0.5-2; more preferably, the polyethylene glycol has a molecular weight of 400-4000 g / mol; more preferably, the fatty alcohol is a fatty alcohol with 3-12 carbon atoms, even more preferably, the fatty alcohol has 1-3 hydroxyl groups; and / or more preferably, the polyacrylamide has a molecular weight of 50000-5000000 g / mol.

19. A fiber fabric carrier for cell culture, characterized in that the fiber fabric carrier is made of polyester fiber fabric according to any one of claims 12 to 13 or is prepared by the method for preparing polyester fiber fabric according to any one of claims 14 to 18.

20. The fiber fabric carrier according to claim 19, wherein the fiber fabric carrier is prepared by pretreating and / or sterilizing the polyester fiber fabric; preferably, the pretreatment includes soaking the polyester fiber fabric in a hydrogen peroxide solution; and / or preferably, the sterilization includes at least one of ethylene oxide sterilization, high-temperature steam sterilization, and irradiation sterilization, more preferably high-temperature steam sterilization.

21. The fiber fabric carrier according to any one of claims 19 to 20, characterized in that it comprises multiple layers, preferably 2 to 7 layers of polyester fiber fabric according to any one of claims 12 to 13; preferably, weld lines are present between the polyester fiber fabrics, preferably the shortest distance between any two weld lines is >0.5 mm, more preferably, the weld lines are straight lines.

22. The fiber fabric carrier according to any one of claims 19 to 21, characterized in that the fiber fabric carrier is circular, elliptical or rectangular; preferably, the diameter of the circle, the major axis of the ellipse or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm.

23. A method for preparing the fiber fabric carrier according to any one of claims 19 to 22, characterized in that the method includes: pretreating and / or sterilizing the polyester fiber fabric; preferably, the pretreating includes soaking the polyester fiber fabric in a hydrogen peroxide solution; and / or preferably, the sterilizing includes at least one of ethylene oxide sterilization, high-temperature steam sterilization, and irradiation sterilization, more preferably high-temperature steam sterilization.

24. Use of the composite polyester fiber according to any one of claims 1 to 6 or the composite polyester fiber prepared by the method according to any one of claims 7 to 11 for preparing fiber fabric carrier for cell culture.

25. Use of the polyester fiber fabric according to any one of claims 12 to 13 or the fiber fabric carrier according to any one of claims 19 to 22 in cell culture, preferably the cells are adherent cells, more preferably Vero cells and / or L929 cells.

26. A method for culturing cells, characterized in that the method includes: sterilizing the polyester fiber fabric according to any one of claims 12 to 13 and then placing it in a culture medium as cell carrier, or placing the fiber fabric carrier according to any one of claims 19 to 22 in a culture medium as cell carrier, and inoculating cells on the cell carrier and culturing in vitro, preferably the cells are adherent cells, more preferably Vero cells and / or L929 cells.

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