Assay for determination of functional immunity against salmonella

The optimized SBA method addresses the inefficiencies of existing antibody response evaluation by using specific buffers and growth phases, achieving rapid and precise determination of serum bactericidal activity against Salmonella.

GB2700365APending Publication Date: 2026-01-28SERUM INST OF INDIA PTE LTD
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Patent Information

Application Number
GB2025002938
Authority / Receiving Office
GB · GB
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-02-29
Filing Date
2025-02-28
Publication Date
2026-01-28

AI Technical Summary

Technical Problem

Existing methods for evaluating functional antibody response induced by immunogenic compositions are time-consuming, costly, and lack precision and consistency in determining serum bactericidal activity against Salmonella.

Method used

A method involving the preparation of media and buffer, bacterial stock, and serum bactericidal activity assay (SBA) using Luria Bertani agar plates to accurately determine antibody titre, optimized for Salmonella Typhi and Paratyphi, with specific buffer compositions and bacterial growth phases to enhance accuracy and reduce time.

Benefits of technology

The method provides a cost-effective, rapid, and precise evaluation of functional antibodies, reducing assay time from 3 days to 2 days while ensuring accurate determination of serum bactericidal activity.

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Abstract

Method of performing SBA assay comprising: preparing media and buffer; preparing bacterial stock with a mother culture stock, media and buffer; diluting bacterial stock with buffer to obtain a dilute
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