Tagged exoglycosidase enzymes and immobilized glycan sequencing approach

Abstract

The invention discloses pure, soluble, and functional exoglycosidase enzymes that can be produced in high yield and cost-effective way using bacterial expression systems. Peptide tag, preferably HIS-tag is used for potential immobilization to ensure the best accessibility to the active sites of the enzymes targeting automation purposes and special workflows. The invention provides rapid enzymatic digestion performance both in aqueous phase and in immobilized form. Immobilized enzymes allow long term storage and ready to use pre-mixing. The inventive immobilization approach opens up the possibility for automation and for meeting special experimental needs where the immobilization of the enzymes is key.